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Pituitary
Bovine
Calf, male, less then 6 months old
Hassan, H. and Merkel, R.
Perifusion Model System to Culture Bovine Hypothalamic Slices In Series with Dispersed Anterior Pituitary Cells ,
In Vitro Cell Dev Biol
 30A , 435 , 1994
Cells
Pituitary
Enzymes
Collagenase : 0.3%
Medium
DMEM
Pituitary
Bovine
Bovine
Mason, W. and Ingram, C.
Techniques for Studying the Role of Electrical Activity in Control of Secretion by Normal Anterior Pituitary Cells , Vol. 124 , 207 , 1986
Cells
Pituitary
Enzymes
Collagenase : 0.1%
Medium
EBSS, CMF
Pituitary
Bovine
Bovine
Ferrara, N. , Goldsmith, P. , Fujii, D. and Weiner, R.
Culture and Characterization of Follicular Cells of the Bovine Anterior Pituitary and Pars Tuberalis , Vol. 124 , 245 , 1986
Cells
Follicular, anterior pituitary and pars tuberalis
Enzymes
Deoxyribonuclease I : 200 µg/ml
Medium
HBSS, CMF
Pituitary
Bovine
Calf, male, 1-6 week old
Ridgway, E. , Klibanski, A. , Marorana, M. , Milbury, P. , Kieffer, J. and Chin, W.
The Effect of Somatostatin on the Release of Thyrotropin and its Subunits from Bovine Anterior Pitituary Cells in Vitro ,
Endocrinology
 112 (6) , 1937 , 1983
Cells
Pitituary
Enzymes
Hyaluronidase : 0.1%
Medium
DMEM
Pituitary
Mouse
Mouse
Perez Millain, M. , Brinkmeier, M. , Mortensen, A. and Camper, S.
PROP1 Triggers Epithelial-Mesenchymal Transition-Like Process in Pituitary Stem Cells. ,
Elife
 5 , 2016
Cells
Pituitary
Enzymes
Medium
HBSS
Pituitary
Mouse
Mouse
Pyczek, J. , Buslei, R. , Schult, D. , Holsken, A. , Buchfelder, M. , Hess, I. , Hahn, H. and Uhmann, A.
Hedgehog Signaling Activation Induces Stem Cell Proliferation and Hormone Release in the Adult Pituitary Gland. ,
Sci Rep
 6 , 24928 , 2016
Cells
Pituitary
Enzymes
Medium
DMEM
Pituitary
Mouse
Mouse, male
Steveson Tami C, Ciccotosto Giuseppe D, Ma Xin-Ming, Mueller Gregory P, Mains Richard E, Eipper Betty A.
Menkes protein contributes to the function of peptidylglycine alpha-amidating monooxygenase ,
Endocrinology
 144 , 188-200 , 2003
Cells
Pituitary
Enzymes
Collagenase : 0.4%
Hyaluronidase : 0.1%
Trypsin : 0.3%
Medium
DMEM/Han's F12
Pituitary
Ovine
Ovine, adult
Xu Ruwei, Wang Qinling, Yan Ming, Hernandez Maria, Gong Changhong, Boon WahChin, Murata Yoko, Ueta Yoichi, Chen Chen.
Orexin-A augments voltage-gated Ca2+ currents and synergistically increases growth hormone (GH) secretion with GH-releasing hormone in primary cultured ovine somatotropes ,
Endocrinology
 143 , 4609-19 , 2002
Cells
Somatotropes
Enzymes
Medium
Medium 199
Pituitary
Rat
Rat, male, 12-15 week
Akieda-Asai, S. , Zaima, N. , Ikegami, K. , Kahyo, T, Yao, I. , Hatanaka, T. , Iemura, S. , Sugiyama, R. , Yokozeki, T. , Eishi, Y. , Koike, M. , Ikeda, K. , Chiba, T. , Yamaza, H. , Shimokawa, I. , Song, S. , Matsuno, A. , Mizutani, A. , Sawabe, M. Chao, N. and Tanaka, M.
SIRT1 Regulates Thyroid-Stimulating Hormone Release by Enhancing PIP5K gamma Activity through Deacetylation of Specific Lysine Residues in Mammals. ,
PLoS ONE
 5 , e11755 , 2010
Cells
Pituitary
Enzymes
Medium
DMEM
Pituitary
Rat
Rat, Wistar, male, 2 month old
Zhou, X. , De Schepper, J. , De Craemer, D. , Delhase, M. , Gys, G. , Smitz, J. and Hooghe-Peters, E.
Pituitary Growth Hormone Release and Gene Expression in Cafeteria-Diet-Induced Obese Rats ,
J Endocrinol
 159 , 165 , 1998
Cells
Pituitary
Enzymes
Trypsin : 0.1%
Medium
DMEM
Pituitary
Rat
Rat, SD, female, 200 - 250 g
D'Emden, M. and Wark, J.
Culture Requirements for Optimal Expression of 1,25-Dihydroxyvitamin D3-Enhanced Thyrotropin Secretion ,
In Vitro Cell Dev Biol
 27 , 197 , 1991
Cells
Anterior pituitary gland
Enzymes
Trypsin : 0.1%
Medium
EBSS, CMF
Pituitary
Rat
Rat
Wilfinger, W. , Larsen, W. , Downs, T. and Wilbur, D.
An In Vitro Model for Studies of Intercellular Communication in Cultured Rat Anterior Pituitary Cells ,
Tissue Cell
 16 (4) , 483 , 1984
Cells
Pituitary
Enzymes
Trypsin : 0.3 %
Medium
HEPES
Pituitary
Rat
Rat, SD, male, 250-450 g
Portanova, R. , Smith, D. and Sayers, G.
A Trypsin for the Preparation of Isolated Rat Anterior Pituitary ,
Proc Soc Exp Biol Med
 133 , 573 , 1970
Cells
Anterior pituitary
Enzymes
Trypsin : 0.25%
Medium
Krebs
Prostate
Human
Human
Taylor, R. , Toivanen, R. , Frydenberg, M. , Pedersen, J. , Harewood, L. , Collins, A. , Maitland, N. and Risbridger, G.
Human Epithelial Basal Cells are Cells of Origin of Prostate Cancer, Independent of CD133 Status. ,
Stem Cells
 30 , 1087 , 2012
Cells
Prostatic cancer fibroblasts
Enzymes
Collagenase : 225 u/ml
Hyaluronidase : 125 u/ml
Medium
RPMI 1640
Prostate
Human
Human, 52-56 yr
Le Hanh, Arnold Julia T, McFann Kimberly K, Blackman Marc R.
DHT and testosterone, but not DHEA or E2, differentially modulate IGF-I, IGFBP-2, and IGFBP-3 in human prostatic stromal cells ,
Am J Physiol/Endo
 290 , E952-60 , 2006
Cells
Prostatic stromal cells
Enzymes
Medium
DMEM/F-12
Prostate
Human
Human
Nakashiro Koh-Ichi, Hara Shingo, Shinohara Yuji, Oyasu Miho, Kawamata Hitoshi, Shintani Satoru, Hamakawa Hiroyuki, Oyasu Ryoichi.
Phenotypic switch from paracrine to autocrine role of hepatocyte growth factor in an androgen-independent human prostatic carcinoma cell line, CWR22R ,
Am J Pathol
 165 , 533-40 , 2004
Cells
Prostate stromal cells
Enzymes
Medium
RPMI 1640
Prostate
Human
Human, fetal
Levine AC, Liu XH, Greenberg PD, Eliashvili M, Schiff JD, Aaronson SA, Holland JF, Kirschenbaum A.
Androgens induce the expression of vascular endothelial growth factor in human fetal prostatic fibroblasts ,
Endocrinology
 139 , 4672-8 , 1998
Cells
Prostatic fibroblasts
Enzymes
Medium
DMEM/F12
Prostate
Mouse
Mouse, 6-8 week
Burger, P. , Gupta, R. , Xiong, X. , Ontiveros, C. , Salm, S. , Moscatelli, D. and Wilson, E.
High Aldehyde Dehydrogenase Activity: A Novel Functional Marker of Murine Prostate Stem/Progenitor Cells. ,
Stem Cells
 27 , 2220-8 , 2009
Cells
Prostate epithelial/stem
Enzymes
Trypsin : 0.05%
Medium
HBSS
Prostate
Mouse
Mouse, 2 week
Imamov Otabek, Morani Andrea, Shim Gil-Jin, Omoto Yoko, Thulin-Andersson Christina, Warner Margaret, Gustafsson Jan-Ake.
Estrogen receptor beta regulates epithelial cellular differentiation in the mouse ventral prostate ,
Proc Natl Acad Sci U S A
 101 , 9375-80 , 2004
Cells
Prostatic epithelial
Enzymes
Collagenase Type 3 : 170 u/ml
Medium
DMEM
Prostate
Mouse
Mouse, male
Dubey P, Wu H, Reiter RE, Witte ON.
Alternative pathways to prostate carcinoma activate prostate stem cell antigen expression ,
Cancer Res
 61 , 3256-61 , 2001
Cells
Prostatic stem
Enzymes
Collagenase Type 1 : 170 u/ml
Medium
DMEM
Skin
Canine
Canine
Zheng, Y. , Nace, A. , Chen, W. , Watkins, K. , Sergott, L. , Homan, Y. , Vandeberg, J. , Breen, M. and Stenn, K.
Mature Hair Follicles Generated from Dissociated Cells: A Universal Mechanism of Folliculoneogenesis. ,
Dev Dyn
 239 , 2619-26 , 2010
Cells
Epidermal
Enzymes
Collagenase : 0.35%
Medium
DMEM
Skin
Frog
Frog
Nishikawa, A. , Shimizu-Nishikawa, K. and Miler, L.
Isolation, characterization, and in vitro culture of larval and adult epidermal cells of the frog Xenopus laevis ,
In Vitro Cell Dev Biol
 26 , 1128 , 1990
Cells
Epidermal
Enzymes
Trypsin : 0.18%
Medium
Barth's solution, CMF
Skin
Goat
Goat
Deng, Y. , Hu, J. and Athanasiou, K.
Isolation and Chondroinduction of a Dermis-Isolated, Aggrecan-Sensitive Subpopulation with High Chondrogenic Potential. ,
Arthritis Rheum
 56 , 168 , 2007
Cells
Dermis isolated aggrecan sensitive
Enzymes
Medium
DMEM
Skin
Human

Human

Burja, B. , Paul, D. , Tastanova, A. , Edalat, S. , Gerber, R. , Houtman, M. , Elhai, M. , Bürki, K. , Staeger, R. , Restivo, G. , Lang, R. , Sodin-Semrl, S. , Lakota, K. , Tomšič, M. , Levesque, M. , Distler, O. , Rotar, �. , Robinson, M. and Frank-Bertoncelj, M.
An Optimized Tissue Dissociation Protocol for Single-Cell RNA Sequencing Analysis of Fresh and Cultured Human Skin Biopsies. , Front Cell Dev Biol Vol. 10 , 872688 , 2022
Cells
Skin
Enzymes
Neutral Protease : 2.4 u/ml
Collagenase Type 4 : 1,000 u/ml
Medium
RPMI
Skin
Human
Human
Tan, J. , Jaafar, F. and Makpol, S.
Proteomic Profiling of Senescent Human Diploid Fibroblasts Treated with gamma-tocotrienol. ,
BMC Complement Altern Med
 18 , 314 , 2018
Cells
Diploid fibroblasts
Enzymes
Medium
DMEM
Skin
Human
Human
Botting, Ra. , Bertram, K. , Baharlou, H. , Sandgren, K. , Fletcher, J. , Rhodes, J. , Rana, H. , Plasto, T. , Wang, X. , Lim, J. , Barnouti, L. , Kohout, M. , Papadopoulos, T. , Merten, S. , Olbourne, N. and Cunningham, A.
Phenotypic and Functional Consequences of Different Isolation Protocols on Skin Mononuclear Phagocytes. ,
J Leukoc Biol
 101 , 1393-1403 , 2017
Cells
Mononuclear phagocytes
Enzymes
Neutral Protease : 1 u/ml
Collagenase : 200 u/ml
Trypsin : 0.5%
Medium
RPMI-1640
Skin
Human
Human
Chen, Z. , Wang, X. , Jin, T. , Wang, Y. , Hong, C. , Tan, L. , Dai, T. , Wu, L. , Zhuang, Z. and Shi, C.
Increase in the Radioresistance of Normal Skin Fibroblasts but not Tumor Cells by Mechanical Injury. ,
Cell Death Dis
 8 , e2573 , 2017
Cells
Fibroblasts
Enzymes
Medium
DMEM
Skin
Human
Human
Douvaras, P. , Wang, J. , Zimmer, M. , Hanchuk, S. , O'Bara, M. , Sadiq, S. , Sim, F. , Goldman, J. and Fossati, V.
Efficient Generation of Myelinating Oligodendrocytes from Primary Progressive Multiple Sclerosis Patients by Induced Pluripotent Stem Cells. ,
Stem Cell Reports
 3 , 250 , 2014
Cells
Skin fibroblasts
Enzymes
Collagenase Type 1 : 1000 u/ml
Medium
DMEM
Skin
Human
Human
Cheuk, S. , Wiken, M. , Blomqvist, L. , Nylen, S. , Talme, T. and Stahle, M.and Eidsmo, L.
Epidermal Th22 and Tc17 Cells Form a Localized Disease Memory in Clinically Healed Psoriasis. ,
J Immunol
 192 , 3111 , 2014
Cells
Skin
Enzymes
Medium
RPMI 1640
Skin
Human
Human
Cerny, D. , Haniffa, M. , Shin, A. , Bigliardi, P. , Tan, B. , Lee, B. , Poidinger, M. , Tan, E. , Ginhoux, F. and Fink, K.
Selective Susceptibility of Human Skin Antigen Presenting Cells to Productive Dengue Virus Infection. ,
PLoS Pathog
 10 , e1004548 , 2014
Cells
Skin
Enzymes
Medium
RPMI
Skin
Human
Human, adult
Nagel, S. , Rohr, F. , Weber, C. , Kier, J. , Siemers, F. , Kruse, C. , Danner, S. , Brandenburger, M. and Matthiessen, A.
Multipotent Nestin-Positive Stem Cells Reside in the Stroma of Human Eccrine and Apocrine Sweat Glands and can be Propagated Robustly In Vitro. ,
PLoS ONE
 8 , e78365 , 2013
Cells
Epidermal stem cells
Enzymes
Neutral Protease : 2 u/ml
Trypsin : 2.5%
Medium
DMEM
Skin
Human
Human, female, 18-66 yr
Huschtscha, L. , Napier, C. , Noble, J. , Bower, K. , Au, A. , Campbell, H. , Braithwaite, A. and Reddel, R.
Enhanced Isolation of Fibroblasts From Human Skin Explants. ,
Biotechniques
 53 , 239 , 2012
Cells
Fibroblasts
Enzymes
Trypsin : 0.2%
Medium
DMEM
Skin
Human
Human
Carriel, V. , Garzon, I. , Jimenez, J. , Oliveira, A. , Arias-Santiago, S. , Campos, A. , Sanchez-Quevedo, M. and Alaminos, M.
Epithelial and Stromal Developmental Patterns in a Novel Substitute of the Human Skin Generated with Fibrin-Agarose Biomaterials. ,
Cells Tissues Organs
 196 , 1-12 , 2012
Cells
Fibroblasts and keratinocytes
Enzymes
Medium
DMEM
Skin
Human
Human
Karumbayaram, S. , Lee, P. , Azghadi, S. , Cooper, A. , Patterson, M. , Kohn, D. , Pyle, A. , Clark, A. , Byrne, J. , Zack, J. , Plath, K. and Lowry, W.
From Skin Biopsy to Neurons Through a Pluripotent Intermediate Under Good Manufacturing Practice Protocols ,
Stem Cells Transl Med
 1 , 36 , 2012
Cells
Fibroblasts
Enzymes
Medium
HBSS
Skin
Human
Human
Zheng, Y. , Nace, A. , Chen, W. , Watkins, K. , Sergott, L. , Homan, Y. , Vandeberg, J. , Breen, M. and Stenn, K.
Mature Hair Follicles Generated from Dissociated Cells: A Universal Mechanism of Folliculoneogenesis. ,
Dev Dyn
 239 , 2619-26 , 2010
Cells
Epidermal
Enzymes
Collagenase : 0.35%
Medium
DMEM
Skin
Human
Human
Zhang, Q. , Yamaza, T. , Kelly, AP, Shi, S. , Wang, S. , Brown, J. , Wang, L. , French, S. , Shi, S. and Le, A.
Tumor-like Stem Cells Derived from Human Keloid are Governed by the Inflammatory Niche Driven by IL-17/IL-6 Axis. ,
PLoS ONE
 4 , e7798 , 2009
Cells
Skin and keloid progenitor
Enzymes
Medium
PBS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.