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Tissue References Search Tool

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Skin
Human
Human, 6-12 year
Chen, F. , Zhang, W. , Bi, D. , Liu, W. , Wei, X. , Chen, F. , Zhu, L. , Cui, L. and Cao, Y.
Clonal Analysis of Nestin(-) Vimentin(+) Multipotent Fibroblasts Isolated from Human Dermis. ,
J Cell Sci
 120 , 2875 , 2007
Cells
Dermal fibroblasts
Enzymes
Medium
DMEM
Skin
Human
Human
Yu Hong, Fang Dong, Kumar Suresh M, Li Ling, Nguyen Thiennga K, Acs Geza, Herlyn Meenhard, Xu Xiaowei.
Isolation of a novel population of multipotent adult stem cells from human hair follicles ,
Am J Pathol
 168 , 1879-88 , 2006
Cells
Hair follicular epithelial
Enzymes
Trypsin : 0.25%
Medium
DMEM/F-12
Skin
Human
Human
Clark, R. , Chong, B. , Mirchandani, N. , Yamanaka, K. , Murphy, G. , Dowgiert, R. and Kupper, T.
A Novel Method for the Isolation of Skin Resident T cells from Normal and Diseased Human Skin. ,
J Invest Dermatol
 126 , 1059 , 2006
Cells
fibroblasts
Enzymes
Collagenase : 0.5%
Trypsin : 0.25%
Medium
DMEM/F12
Skin
Human
Human, adult
Wang, H. , Van Blitterswijk, C. , Bertrand-De Haas, M. , Schuurman, A. and Lamme, E.
Improved Enzymatic Isolation of Fibroblasts for the Creation of Autologous Skin Substitutes. ,
In Vitro Cell Dev Biol Anim
 40 , 268 , 2004
Cells
Dermal fibroblasts
Enzymes
Collagenase : 0.5-1.0%
Medium
DMEM
Skin
Human
Human, neonatal
Li, A. , Pouliot, N. , Redvers, R. and Kaur, P.
Extensive tissue-regenerative capacity of neonatal human keratinocytes stem cells and their progeny ,
J Clin Invest
 113 , 390-400 , 2004
Cells
Keratinocytes
Enzymes
Medium
DMEM
Skin
Human
Human
Babina Magda, Guhl Sven, Starke Andrae, Kirchhof Loreen, Zuberbier Torsten, Henz Beate M.
Comparative cytokine profile of human skin mast cells from two compartments--strong resemblance with monocytes at baseline but induction of IL-5 by IL-4 priming ,
J Leukoc Biol
 75 , 244-52 , 2004
Cells
Mast cells
Enzymes
Medium
RPMI
Skin
Human
Human
Tuan Tai-Lan, Wu Huayang, Huang Eunice Y, Chong Sheree S N, Laug Walter, Messadi Diana, Kelly Paul, Le Anh.
Increased plasminogen activator inhibitor-1 in keloid fibroblasts may account for their elevated collagen accumulation in fibrin gel cultures ,
Am J Pathol
 162 , 1579-89 , 2003
Cells
Fibroblasts
Enzymes
Trypsin : 0.25%
Medium
DMEM
Skin
Human
Human
Supp Dorothy M, Wilson-Landy Kaila, Boyce Steven T.
Human dermal microvascular endothelial cells form vascular analogs in cultured skin substitutes after grafting to athymic mice ,
FASEB J
 16 , 797-804 , 2002
Cells
Keratinocytes, fibroblasts, endothelial
Enzymes
Neutral Protease : see reference
Collagenase : see reference
Medium
see reference
Skin
Human
Human, adult
Baudoux, B. , Castanares-Zapatero, D. , Leclercq-Smekens, M. , Berna, N. and Poumay, Y.
The Tetraspanin CD9 Associates with the Integrin a6B4 in Cultured Human Epidermal Keratinocytes and is Involved in Cell Motility ,
Eur J Cell Biol
 79 , 41 , 2000
Cells
Keratinocytes
Enzymes
Medium
PBS
Skin
Human
Human
Hybbinette S, Bostrom M, Lindberg K.
Enzymatic dissociation of keratinocytes from human skin biopsies for in vitro cell propagation ,
Exp Dermatol
 8 , 30-8 , 1999
Cells
Keratinocytes
Enzymes
Trypsin : 0.17%
Thermolysin : 0.05%
Medium
see reference
Skin
Human
Human
Grutzkau A, Kruger-Krasagakes S, Baumeister H, Schwarz C, Kogel H, Welker P, Lippert U, Henz BM, Moller A.
Synthesis, storage, and release of vascular endothelial growth factor/vascular permeability factor (VEGF/VPF) by human mast cells: implications for the biological significance of VEGF206 ,
Mol Biol Cell
 9 , 875-84 , 1998
Cells
Human skin mast cells
Enzymes
Collagenase Type 1 : see reference
Medium
PBS
Skin
Human
Human
Reece J C, Handley A J, Anstee E J, Morrison W A, Crowe S M, Cameron P U.
HIV-1 selection by epidermal dendritic cells during transmission across human skin ,
J Exp Med
 187 , 1623-31 , 1998
Cells
Epidermal
Enzymes
Medium
PBS
Skin
Human
Human
Judd, D. , Battista, P. and Behm, D.
Culture of Human Keratinocytes in Defined Serum-Free Medium ,
Focus
 19 (1) , 2 , 1997
Cells
Keratinocytes
Enzymes
Trypsin : 0.05%
Medium
Dulbecco's PBS
Skin
Human
Human
Harley, C. and Sherwood, S.
, Methods in Molecular Biology, Basic Cell Culture Protocols, 2nd ed. Vol. 75 , Pollard, J., and Walker, J. , Humana Press , 23 , 1997
Cells
Epidermis plus dermis
Abdomen or inner forearm
Enzymes
Trypsin : 0.125%
Medium
DMEM, M199
Skin
Human
Human
Regnier, M.
Culture of Human Karatinocytes , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons Ltd , 11B:4.1 , 1995
Cells
Keratinocytes
Enzymes
Trypsin : 0.25%
Medium
DMEM
Skin
Human
Human, adult, female, breast skin
Hirel, B. , Chesne, C. , Pailgheret, J. and Guillouzo, A.
Expression of Differentiation Markers in Human Adult Keratinocytes Cultured in Submerged Conditions ,
In Vitro Cell Dev Biol
 30A , 372 , 1994
Cells
Keratinocytes
Enzymes
Trypsin : 0.25%
Medium
PBS
Skin
Human
Human, neonatal foreskin
Hansbrough, J. , Cooper, M. , Cohen, R. , Spielvogel, R. , Greenleaf, G. , Bartel, R. and Naughton, G.
Evaluation of a Biodegradable Matrix Containing Cultured Human Fibroblasts as a Dermal Replacement Beneath Meshed Skin Grafts on Athymic Mice ,
Surgery
 111 (1) , 438 , 1992
Cells
Fibroblasts
Enzymes
Trypsin : 0.25%
Medium
DMEM
Skin
Human
Human, 18-50 years
Bell, C. and Quinton, P.
Effects of Media Buffer Systems on Growth and Electrophysiologic Characteristics of Cultured Sweat Duct Cells ,
In Vitro Cell Dev Biol
 27 , 47 , 1991
Cells
Sweat duct
Enzymes
Collagenase : 0.03%
Medium
MEM
Skin
Human
Human
Limat, A. , Hunziker, T. , Boillat, C. , Noser, F. and Wiesmann, U.
Postmitotic Human Dermal Fibroblasts Preserve Intact Feeder Properties for Epithelial Cell Growth After Long-Term Cryopreservation ,
In Vitro Cell Dev Biol
 26 , 709 , 1990
Cells
Fibroblasts
Enzymes
Trypsin : 0.25%
Medium
CMF solution
Skin
Human
Human
Krouse, M. , Hagiqara, G. , Chen, J. , Lewiston, N. and Wine, J.
Ion Channels in Normal Human and Cystic Fibrosis Sweat Gland Cells ,
Am J Physiol
 257 , C129 , 1989
Cells
Sweat gland
Enzymes
Medium
(see reference)
Skin
Human
Human, ages 16-30
Pedersen, P.
Human Sweat Duct Cells in Primary Culture. Basic Bioelectric Properties of Cultures Derived From Normals and Patients with Cystic Fibrosis ,
In Vitro Cell Dev Biol
 25 (4) , 342 , 1989
Cells
Human sweat duct
Enzymes
Medium
RPMI 1640
(see reference)
Skin
Human
Human
Peacocke, M. , Yaar, M. , Mansur, C. , Chao, M. and Gilchrest, B.
Induction of Nerve Growth Factor Receptors on Cultured Human Melanocytes ,
Proc Natl Acad Sci U S A
 85 , 5282 , 1988
Cells
Melanocytes, skin/ foreskin
Enzymes
Trypsin : 0.25%
Medium
PBS
Skin
Human
Human
Lee, C. , Carpenter, F. , Coaker, T. and Kealey, T.
The Primary Culture of Epithelia From the Secretory Coil and Collecting Duct of Normal Human and Cystic Fibrotic Eccrine Sweat Glands ,
J Cell Sci
 83 , 103 , 1986
Cells
Human sweat glands
Enzymes
Collagenase : 0.2%
Medium
HBSS
Skin
Human
Human
Dover, R. and Potten, C.
Cell Cycle Kinetics of Cultured Human Epidermal Keratinocytes ,
J Invest Dermatol
 80 , 423 , 1983
Cells
Keratinocytes
Enzymes
Trypsin : 0.25%
Medium
DMEM
Skin
Human
Human, 18-30 years, male, female
McCoy, B. , Galdun, J. and Cohen, I.
Effects of Density and Cellular Aging On Collagen Synthesis and Growth Kinetics in Keloid and Normal Skin Fibroblasts ,
In Vitro
 18 (1) , 79 , 1982
Cells
Fibroblasts
Enzymes
Trypsin : 0.1%
Medium
HBSS
Skin
Human
Human, adult
Alitalo K, Kuismanen E, Myllyla R, Kiistala U, Asko-Seljavaara S, Vaheri A.
Extracellular matrix proteins of human epidermal keratinocytes and feeder 3T3 cells ,
J Cell Biol
 94 , 497-505 , 1982
Cells
Epidermal keratinocytes
Enzymes
Trypsin : 0.25%
Collagenase : 0.2%
Medium
Eagle's MEM
Skin
Human
Human, fetal
Davies, P. and Kerr, C.
Modification of LDL Metabolism by Growth Factors in Cultured Vascular Cells and Human Skin Fibroblasts ,
Biochim Biophys Acta
 712 , 26 , 1982
Cells
Smooth muscle, fibroblasts
Enzymes
Trypsin : 0.055%
Medium
DMEM
Skin
Human
Human, newborn
Liu, S. , Eaton, M. and Karasek, M.
Growth Characteristics of Human Epidermal Keratinocytes from Newborn Foreskin in Primary and Serial Cultures ,
In Vitro
 15 (10) , 813 , 1979
Cells
Keratinocytes
Enzymes
Trypsin : 0.3%
Medium
DMEM
Skin
Human
Human
Rheinwald, J. and Green, H.
Serial Cultivation of Strains of Human Epidermal Kerotinocytes: The Formation of Keratinizing Colonies from Single Cells ,
Cell
 6 , 331 , 1975
Cells
Keratinocytes
Enzymes
Trypsin : 0.25%
Medium
(see reference)
Skin
Mouse
Mouse
Spidale, N. , Malhotra, N. , Frascoli, M. , Sylvia, K. , Miu, B. , Freeman, C. , Stadinski, B. , Huseby, E. and Kang, J.
Neonatal-Derived IL-17 Producing Dermal gd T Cells are Required to Prevent Spontaneous Atopic Dermatitis ,
Elife
 9 , 5118 , 2020
Cells
Keratinocytes
Enzymes
Medium
DMEM
Skin
Mouse
Mouse
Blanco, S. , Bandiera, R. , Popis, M. , Hussain, S. , Lombard, P. , Aleksic, J. , Sajini, A. , Tanna, H. , Cortes-Garrido, R. , Gkatza, N. , Dietmann, S. and Frye, M.
Stem Cell Function and Stress Response are Controlled by Protein Synthesis. ,
Nature
 534 , 335-40 , 2016
Cells
Keratinocytes
Enzymes
Medium
Low calcium containing media
Skin
Mouse
Mouse
Rinkevich, Y. , Walmsley, G. , Hu, M. , Maan, Z. , Newman, A. , Drukker, M. , Januszyk, M. , Krampitz, G. , Gurtner, G. , Lorenz, H. , Weissman, I. and Longaker, M.
Identification and Isolation of a Dermal Lineage with Intrinsic Fibrogenic Potential. ,
Science
 348 , aaa2151 , 2015
Cells
Dermal fibroblasts
Enzymes
Elastase : 0.012%
Medium
DMEM
Skin
Mouse
Mouse
Cerny, D. , Haniffa, M. , Shin, A. , Bigliardi, P. , Tan, B. , Lee, B. , Poidinger, M. , Tan, E. , Ginhoux, F. and Fink, K.
Selective Susceptibility of Human Skin Antigen Presenting Cells to Productive Dengue Virus Infection. ,
PLoS Pathog
 10 , e1004548 , 2014
Cells
Skin
Enzymes
Medium
RPMI
Skin
Mouse
Mouse
Jensen, U. , Ghazizadeh, S. and Owens, D.
Isolation and Characterization of Cutaneous Epithelial Stem Cells. ,
Methods Mol Biol
 989 , 61-9 , 2013
Cells
Epithelial stem
Enzymes
Trypsin : 0.25%
Medium
HBSS
Skin
Mouse
Mouse
Gu, D. , Fan, Q. and Xie, J.
Cell Population Analyses During Skin Carcinogenesis. ,
J Vis Exp
 , e50311 , 2013
Cells
Epidermal
Enzymes
Medium
DMEM
Skin
Mouse
Mouse
Antsiferova, M. , Martin, C. , Huber, M. , Feyerabend, T. , Forster, A. , Hartmann, K. , Rodewald, H. , Hohl, D. and Werner, S.
Mast Cells are Dispensable for Normal and Activin-Promoted Wound Healing and Skin Carcinogenesis. ,
J Immunol
 191 , 6147-55 , 2013
Cells
Mast
Enzymes
Medium
IMDM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.