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Tissue References Search Tool

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Heart
Rat
Rat, 1-2 day
Reinecke H, Zhang M, Bartosek T. and Murry CE.
Survival, integration, and differentiation of cardiomyocyte grafts: a study in normal and injured rat hearts ,
Circulation
 100(2) , 193 , 1999
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 95 u/ml
Pancreatin : 0.06%
Medium
DMEM
Heart
Rat
Rat, Wistar, male
Sambandam N, Abrahani MA, St Pierre E, Al-Atar O, Cam MC. and Rodrigues B.
Localization of lipoprotein lipase in the diabetic heart: regulation by acute changes in insulin ,
Arterioscler Thromb Vasc Biol
 19(6) , 1526 , 1999
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 228 u/ml
Medium
Joklik MEM
Heart
Rat
Rat, adult and neonatal
Richards SM, Jaconi ME, Vassort G. and Puceat M.
A spliced variant of AE1 gene encodes a truncated form of Band 3 in heart: the predominant anion exchanger in ventricular myocytes ,
J Cell Sci
 112 , 1519 , 1999
Cells
Cardioomyocytes
Enzymes
Collagenase Type 4 : 0.12%-adult
Collagenase Type 2 : 0.05%-neonatal
Medium
DMEM/medium 199
Heart
Rat
Rat
Zilberman, A. , Dave, V. , Miano, J. , Olson, E. and Periasamy, M.
Evolutionary Conserved Promoter Region Containing CArg-Like Elements is Crucial for Smooth Muscle Myosin Heavy Chain Gene Expression ,
Circ Res
 82 , 566 , 1998
Cells
Thoracic aorta, smooth muscle
Enzymes
Elastase : 0.02%
Medium
(see reference)
Heart
Rat
Rat, SD, 1 day
Wagner DR, Combes A, McTiernan C, Sanders VJ, Lemster B, Feldman AM.
Adenosine inhibits lipopolysaccharide-induced cardiac expression of tumor necrosis factor-alpha ,
Circ Res
 82 , 47-56 , 1998
Cells
Cardiomyocytes
Enzymes
NCIS kit : per instructions
Medium
DMEM/F-12
Heart
Rat
Rat, SD, 300-350 g
Yu L, Netticadan T, Xu YJ, Panagia V, Dhalla NS.
Mechanisms of lysophosphatidylcholine-induced increase in intracellular calcium in rat cardiomyocytes ,
J Pharmacol Exp Ther
 286 , 1-8 , 1998
Cells
Cardiomyocytes
Enzymes
Medium
see reference
Heart
Rat
Rat, SD, female, 200g
Westfall M V, Rust E M, Metzger J M.
Slow skeletal troponin I gene transfer, expression, and myofilament incorporation enhances adult cardiac myocyte contractile function ,
Proc Natl Acad Sci U S A
 94 , 5444-9 , 1997
Cells
Ventricular cardiac myocytes
Enzymes
Medium
Krebs-Henseleit buffer
Heart
Rat
Rat, Wistar, 1 day
Kinugawa, K. , Kohmoto, O. , Yao, A. , Serizawa, T. and Takahashki, T.
Cardiac inducible nitric oxide synthase negatively modulates myocardial function in cultured rat myocytes ,
Am J Physiol
 272 , H35-47 , 1997
Cells
Cardiomyoctes
Enzymes
Collagenase Type 2 : 80 u/ml
Medium
HBSS
Heart
Rat
Rat, neonatal, 5-day
Vanwinkle, W. , Snuggs, M. and Buja, L.
Cardiogel: A Biosynthetic Extracellular Matrix for Cardiomyocyte Culture ,
In Vitro Cell Dev Biol Anim
 32 , 478 , 1996
Cells
Cardiomyocytes
Enzymes
Trypsin : 0.05%
Medium
DMEM
Heart
Rat
Rat, SD, fetal and neonatal
Li RK, Mickle DA, Weisel RD, Zhang J, Mohabeer MK.
In vivo survival and function of transplanted rat cardiomyocytes ,
Circ Res
 78 , 283-8 , 1996
Cells
Cardiomyocytes
Enzymes
Collagenase : 0.1%
Trypsin : 0.2%
Medium
PBS
Heart
Rat
Rat, SD, 250-300 g
Sharma VK, Colecraft HM, Wang DX, Levey AI, Grigorenko EV, Yeh HH. and Sheu SS
Molecular and functional identification of m1 muscarinic acetylcholine receptors in rat ventricular myocytes ,
Circ Res
 79(1) , 86 , 1996
Cells
Cardiomyocytes
Enzymes
Medium
Joklik's
Heart
Rat
SD, SHR or WKY
MacGregor, R. , Klein, R. and Bansal, D.
Secretion of Plasminogen Activator Activity from Neonatal Rat Heart Cells is Regulated by Hormones and Growth Factors ,
Ann N Y Acad Sci
 752 , 331 , 1995
Cells
Cardiomyocytes
Enzymes
NCIS kit : per instructions
Medium
HBSS
Heart
Rat
Rat, SD, male, 200-250 g
Laughlin, M. , Schaeffer, M. and Sturek, M.
Effect of Exercise Training on Intracellular Free Ca2+ Transients in Ventricular Myocytes of Rats ,
Am J Physiol
 73 , 1141 , 1992
Cells
Myocytes
Ventricles
Enzymes
Collagenase : 0.07%
Medium
Joklik's MEM
Heart
Rat
Rat, SD, adult, female, 180-220 g
Fischer, Y. , Rose, H. and Kammermeier. H.
Highly Insulin-Responsive Isolated Rat Heart Muscle Cells Yielded By a Modified Isolation Method ,
Life Sci
 49 , 1679 , 1991
Cells
Cardiomyocytes
Enzymes
Collagenase : 0.11%
Medium
(see reference)
Heart
Rat
Rat, SD, female, 60-90 days
Welder, A.A. , Grant, R. , Bradlaw, J. and Acosta, D.
A Primary Culture System of Adult Rat Heart Cells for the Study of Toxicologic Agents ,
In Vitro Cell Dev Biol
 27 , 921 , 1991
Cells
Myocardial
Enzymes
Medium
Joklik's MEM, Kreb's-Henseleit buffer, CF
Heart
Rat
Rat
(also bovine)
Trifaro, J. , Tang, R. and Novas, M.
Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons ,
In Vitro Cell Dev Biol
 26 , 335 , 1990
Cells
Heart
Adrenal chromaffin
Paraneurons
Enzymes
Trypsin : 0.06%
Medium
25mM HEPES buffered Locke's solution, CMF
Heart
Rat
Rat, neonatal, 1-4 days
Marino, T.A. , Walter, R.,Cobb, E. , Palasiuk, M. , Parsons, T. and Mercer, W.E.
Effects of Norepinephrine on Neonatal Rat Cardiocyte Growth and Differentiation ,
In Vitro Cell Dev Biol
 26 , 229 , 1990
Cells
Cardiomyocytes
Enzymes
Medium
Krebs-Henseleit Buffer, CF
Heart
Rat
Rat, adult, female
Nag, A.C. , Lee, M. and Kosiur, J.R.
Adult Cardiac Muscle Cells in Long-Term Serum-Free Culture:Myofibrillar Organization and Expression of Myosin Heavy Chain Isoforms ,
In Vitro Cell Dev Biol
 26 , 464 , 1990
Cells
Myocytes
Enzymes
Medium
Joklik's MEM
Heart
Rat
Rats, AW, late-gestation pregnant females
Engelmann, G. , McTiernan, C. , Gerrity, R. and Samarel, A.
Serum-free Primary Cultures of Neonatal Rat Cardiomyocytes: Cellular and Molecular Applications ,
Technique
 2 (6) , 279 , 1990
Cells
Ventricular cardiomyocytes
Enzymes
Collagenase Type 2 : 0.05 - 0.08%
Medium
See reference
Heart
Rat
Rat, SD, adult, female, 175 - 225 g
Bugaisky, L.B. and Zak, R.
Differentiation of Adult Rat Cardiac Myocytes in Cell Culture ,
Circ Res
 64 , 493 , 1989
Cells
Myocytes
Enzymes
Collagenase : 0.1%
Medium
Joklik's medium
Heart
Rat
Rat, SD, 2-4 day old
Toraason, M. , Luken, M. , Breitenstein, M. , Krueger, J. and Biagini, R.
Comparative Toxicity of Allylamine and Acrolein in Cultured Myocytes and Fibroblasts from Neonatal Rat Heart ,
Toxicology
 56 , 107 , 1989
Cells
Cardiomyocytes
Fibroblasts
Enzymes
Trypsin : 0.1%
Medium
HBSS
Heart
Rat
Rat, SD, male
Cornwell, T. and Lincoln, T.
Regulation of Intracellular Ca2+ Levels in Cultured Vascular Smooth Muscle Cells ,
J Biol Chem
 264 (2) , 1146 , 1989
Cells
Aortic smooth muscle
Enzymes
Trypsin : 0.05%
Medium
DMEM
Heart
Rat
Rat,SD, albino, 10-14 weeks
Berg, I. , Guse, A.H. and Gercken, G.
Carbamoylcholine-Induced Accumulation of Inositol Mono-,Bis-,Tris-, And Tetrakisphosphates In Isolated Cardiac Myocytes From Adult Rats ,
Biochim Biophys Acta
 1010 , 100 , 1989
Cells
Myocytes
Enzymes
Collagenase Type 1 : 100 u/ml and 150 u/ml
Medium
Krebs Ringer bicarbonate buffer
Heart
Rat
Rat, SD, male, 250 - 350 g
De Young, M. , Giannattasio, B. and Scarpa, A.
Isolation of Calcium-Tolerant Atrial And Ventricular Myocytes From Adult Rat Heart , Methods Enzymology Vol. 173 , 662 , 1989
Cells
Myocytes, ventricular
Enzymes
Collagenase Type 1 : 90 and 100 u/ml
Medium
Joklik's solution with and without calcium
Heart
Rat
Rat, SD, male, 250-350 g
Kim, D. and Smith, T.W.
Temporal Variation in Contractile State And [Ca++] in Isolated Adult Rat And Guinea Pig Cardiac Myocytes , Biology of Isolated Adult Cardiac Myocytes , (Clark, Decker, Borg, eds) , Elsevier Science Publishing Co. Inc. , 370 , 1988
Cells
Myocytes, heart
Enzymes
Hyaluronidase : 0.03%
Medium
Bicarbonate-buffered medium
Heart
Rat
Rat, adult, 150-200 g
McMahon, K.K.
A Study of Adult Rat Atrial Myocyte Attachment to Extricellular Matrix Components and Long Term Culture , Biology of Isolated Adult Cardiac Myocytes , (Clark, Decker, Borg, Eds) , Elsevier Science Publishing Co. Inc. , 318 , 1988
Cells
Myocytes, atria
Enzymes
Collagenase Type 1 : 100 u/ml
Medium
Kreb's Ringer bicarbonate - HEPES buffer
Heart
Rat
Rat, SD
Buxton, I.L.O. and Doggwiler, K.O.
Alpha1-Adrenergic Receptor Signal Transduction in the Adult Rat Cardiac Myocyte , Biology of Isolated Adult Cardiac Myocytes , (Clark, Decker, Borg, eds) , 298 , 1988
Cells
Myocytes
Enzymes
Medium
Kreb's Ringer w/ calcium
Heart
Rat
Rat, Wistar, 2-4 day neonates
Grynberg, A. , Degois, M. , Guenot, L. and Athias, P.
Primary Rat Cardiac Cell Culture: Diet of the Mother Rats as a Determinant Parameter of Cardiomyoblast Production from Neonates ,
Biol Cell
 57 , 89 , 1986
Cells
Myoblast, cardiac
Enzymes
Trypsin : 0.1%
Medium
Standardized Medium
(see reference)
Heart
Rat
Rat, SD, timid-pregnant
Freerksen, D. , Schroedl, N. and Hartzell, C.
Control of Enzyme Activity Levels by Serum and Hydrocortisone in Neonatal Rat Heart Cells Cultured in Serum-Free Medium ,
J Cell Physiol
 120 , 126 , 1984
Cells
Myocytes
Enzymes
Trypsin : 2.4 u/ml
Medium
DMEM
Heart
Rat
Rat, SD, female, 12 weeks
Piper, H.M. , Probst, I. , Schwartz, P. , Hutter, F.J. and Spieckermann, P.G.
Culturing of Calcium Stable Adult Cardiac Myocytes ,
J Mol Cell Cardiol
 14 , 397 , 1982
Cells
Myocytes
Enzymes
Medium
Krebs Ringer bicarbonate buffer
Heart
Rat
Rat, male/female, 200-250 g
Nag, A. and Cheng, M.
Adult Mammalian Cardiac Muscle Cells in Culture ,
Tissue Cell
 13 , 515 , 1981
Cells
Muscle
Enzymes
Hyaluronidase : 0.1%
Medium
Krebs Ringer phosphate buffer, CMF
Heart
Rat
Rat, SD, adult, male, 200-250 g
Frangakis, C. , Bahl, J. , McDaniel, H. and Bressler, R.
Tolerance to Physiological Calcium by Isolated Mycocytes from the Adult Rat Heart; An Improved Cellular Preparation ,
Life Sci
 27 , 815 , 1980
Cells
Myocytes
Enzymes
Hyaluronidase : 0.10%
Medium
Joklik MEM, CF
Heart
Rat
Rat, SD, female, 12 wks, 250 g
Powell, T. , Terrar, D.A. and Twist, V.W.
Electrical Properties of Individual Cells Isolated From Adult Rat Ventricular Myocardium ,
J Physiol
 302 , 131 , 1980
Cells
Myocytes
Ventricular myocardium
Enzymes
Medium
Kreb's Ringer bicarbonate buffer
Heart
Rat
Rat, female, adult, 200 - 300 g
Claycomb, W.C. and Palazzo, M.C.
Culture of The Terminally Differentiated Adult Cardiac Muscle Cell: A Light and Scanning Electron Microscope Study ,
Dev Biol
 80 , 466 , 1980
Cells
Myocytes
Enzymes
Medium
Joklik's MEM
Heart
Rat
Rat, 10 wk
Bishop, S. , Oparil, S. , Reynolds, R. and Drummond, J.
Regional Myocyte Size in Normotensive and Spontaneously Hypertensive Rats ,
Hypertension
 1 (4) , 378 , 1979
Cells
Myocytes
Enzymes
Medium
Saline
Heart
Rat
Rat, male, 200-300 g
Farmer, B. , Harris, R. , Jolly, W. , Hathaway, D. , Katzberg, A. , Watanabe, A. , Whitlow, A. and Besch, H.
Isolation and Characterization of Adult Rat Heart Cells ,
Arch Biochem Biophys
 179 , 545 , 1977
Cells
Myocytes
Enzymes
Hyaluronidase : 82 u/ml
Medium
Kreb's Henseleit buffer

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.