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Tissue References Search Tool

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Liver
Rat
Rat, CD strain, albino, male, 140 -180 g
Braun, L. , Mead, J. , Panzica, M. , Mikumo, R. , Bell, G. and Fausto, N.
Transforming Growth Factor B mRNA Increases During Liver Regeneration:A Possible Paracrine Mechanism Of Growth Regulation ,
Proc Natl Acad Sci U S A
 85 , 1539 , 1988
Cells
Hepatocytes and Nonparenchymal
Enzymes
Pronase : 0.1%
Medium
HEPES buffer with calcium
Liver
Rat
Rat, SD, male, 180-200 g
Gupta, C. , Hattori, A. , Betschart, J. , Virji, M. and Shinozuka, H.
Modulation of Epidermal Growth Factor Receptors in Rat Hepatocytes by Two Liver-Promoting Regimens, a Choline-Deficient and a Phenobarbital Diet ,
Cancer Res
 48 , 1162 , 1988
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
MEM
Liver
Rat
Rat, Wistar, female, 17 days
Cotariu, D. , Barr-Nea, L. , Papo, N. and Zaidman, J.
Induction of gamma-Glutamyl Transferase by Dexamethasone in Cultured Rat Hepatocytes ,
Enzyme
 40 , 212 , 1988
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
HBSS
Liver
Rat
Rat, Wistar, male,3 month, 200 g
Kuiper, J. , Zijlstra, F. , Kamps, J. and vanBerkel, T.
Identification of Prostaglandin D2 as the Major Eiconsanoid from Liver Endothelial and Kupffer Cells ,
Biochim Biophys Acta
 959 , 143 , 1988
Cells
Parenchymal
Endothelial
Kupffer
Enzymes
Medium
Krebs Henseleit
Liver
Rat
Rat, Wistar, male, 200 - 300 g
Cai, H. , He, Z. and Ding, Y.
Effects of Monocyte Macrophages Stimulation on Hepatic Lipoprotein Receptors ,
Biochim Biophys Acta
 958 , 334 , 1988
Cells
Parenchymal
Kupffer
Enzymes
Medium
HBSS with CaCl2
Liver
Rat
Rat, 250-350 g
(also guinea-pig & rabbit)
Chang, T. and Chang, C.
Hepatic Uptake of Asialoglycoprotein is Different Among Mammalian Species Due to Different Receptor Distribution ,
Biochim Biophys Acta
 942 , 57 , 1988
Cells
Hepatocytes
Enzymes
Collagenase Type 2 : 166 u/ml
Medium
DMEM
Liver
Rat
Rat, Fisher 344, 150-200 g
Mathis, G. , Walls, S. and Sirica, A.
Biochemical Characteristics of Hyperplastic Rat Bile Ductular Epithelial Cells Cultured "on Top" and "Inside" Different Extracellular Matrix Substitutes ,
Cancer Res
 48 , 6145 , 1988
Cells
Bile ductular epithelial
Enzymes
Collagenase Type 1 : 220 u/ml
Medium
L-15
Liver
Rat
Rat, Wistar, male, 260-310 g
McAbee DD. and Weigel PH
ATP-dependent inactivation and reactivation of constitutively recycling galactosyl receptors in isolated rat hepatocytes ,
Biochemistry
 27 , 2061 , 1988
Cells
Hepatocytes
Enzymes
Medium
HEPES
Liver
Rat
Rat, SD, male, 350-400 g
Rodriguez de Turco, E. and Spitzer, J.
Kinetics of Diacylglycerol Accumulation in Response to Vasopressin Stimulation in Hepatocytes of Continuously Endotoxaemic Rats ,
Biochem J
 253 , 73 , 1988
Cells
Hepatocytes
Enzymes
Medium
HBSS
Liver
Rat
Rat, SD, male, 350 - 450 g
Friedman, S. and Roll, F.
Isolation and Culture of Hepatic Lipocytes,Kupffer Cells, and Sinusoidal Endothelial Cells by Density Gradient Centrifugation with Stractan ,
Anal Biochem
 161 , 207 , 1987
Cells
Lipocytes
Kupffer
Sinusoidal endothelial
Enzymes
Collagenase : 0.015%
Medium
DMEM/Ham's F-12
Liver
Rat
Rat, SD, male
Oka, J. and Weigel, P.
Monensin Inhibits Ligand Dissociation Only Transiently and Partially and distinguishes two galactosyl receptor pathways in isolated rat hepatocytes ,
J Cell Physiol
 133 , 243 , 1987
Cells
Hepatocytes
Enzymes
Collagenase : 100 - 200 µg/g body weight
Medium
Eagle's
Eagle's w/HEPES
HBSS
Liver
Rat
Rat, SD, male, 200-250 g
Kreamer, B. , Staecker, J. , Sawada, N. , Sattler, G. , Hsia, M. and Pitot, H.
Use of Low-Speed, Iso-Density Percoll Centrifugation Method to Increase the Viability of Isolated Rat Hepatocyte Preparations ,
In Vitro Cell Dev Biol
 22 (4) , 201 , 1986
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
HBSS
Liver
Rat
Rat, Fischer, male, 150-250 g
Francavilla, A. , Ove, P. , Polimeno, L. , Sciascia, C. , Coetzee, M. and Starzi, T.
Epidermal Growth Factor and Proliferation in Rat Hepatocytes in Primary Culture Isolation at Different Times after Partial Hepatectomy ,
Cancer Res
 46 , 1318 , 1986
Cells
Hepatocytes
Enzymes
Collagenase Type 1 : 125 - 250 u/ml
Medium
MEM
(see reference)
Liver
Rat
Rat, SD, female, 100-180 g
Schwarz, K. , Lanier, S. , Carter, E. , Homcy, C. and Graham, R.
Rapid Reciprocal Changes in Adrenergic Receptors in Intact Isolated Hepatocytes During Primary Cell Culture ,
Mol Pharmacol
 27 , 200 , 1985
Cells
Hepatocytes
Enzymes
Collagenase : 0.5%
Medium
Krebs Ringer bicarbonate buffer
Liver
Rat
Rat, Lewis, female, 200-250 g
Holstege A, Leser HG, Pausch J, Gerok W.
Uridine catabolism in Kupffer cells, endothelial cells, and hepatocytes ,
Eur J Biochem
 149 , 169-73 , 1985
Cells
Hepatocytes, Kupffer, endothelial
Enzymes
Collagenase : 0.05%
Medium
Gey's BSS
Liver
Rat
Rat, Wistar, adult, male, 150-200 g
Okumura, T. and Saito, K.
Degradation of Prostaglandin E2 in a Primary Culture of Adult Rat Hepatocytes ,
J. Biochem.
 96 , 429 , 1984
Cells
Hepatocytes
Enzymes
Trypsin : 0.005%
Medium
Williams E
Liver
Rat
Rat, SD, male, 270 - 320 g
Brass, E. , Garrity, M. and Robertson, R.
Inhibition of Glucagon-Stimulated Hepatic Glycogenolysis by E-Series Prostaglandins ,
F.E.B.S. Lett.
 169 , 293 , 1984
Cells
Hepatocytes
Enzymes
Collagenase : 0.04%
Medium
Bicarbonate buffer with calcium added
Liver
Rat
Rat, Wistar, male, 3 month old
Nagelkenke, J. , Barto, K. and VanBerkel, T.
In vivo and in vitro uptake and degradation of acetylated low density lipoprotein by rat liver endothelial, Kupffer, and parenchymal cells ,
J Biol Chem
 258 , 12221 , 1983
Cells
Endothelial
Kupffer
Parenchymal
Enzymes
Collagenase : 0.05%
Pronase : 0.25%
Medium
HBSS
Liver
Rat
Rat, Fischer, Lewis and SD, male 10-18 months
Herring, A. , Raychaudhuri, R. , Kelley, S. and Iybe, P.
Repeated Establishment of Diploid Epithelial Cell Cultures from Normal and Partially Hepatectomized Rats ,
In Vitro
 19 , 576 , 1983
Cells
Epithelial
Enzymes
Trypsin : 0.05%
Medium
HBSS CMF
Liver
Rat
Rat, SD, male/female, 250-300 g
Studer, R. and Borle, A.
Differences between Male and Female Rats in the Regulation of Hepatic Glycogenolysis. The Relative Role of Calcium and cAMP in Phosphorylase Activation by Catecholamines ,
J Biol Chem
 257 , 7987 , 1982
Cells
Hepatocytes
Enzymes
Collagenase : 100 u/ml
Medium
Krebs Henseleit bicarbonate buffer
Liver
Rat
Rat, Wistar, 12 day
Malan-Shibley, L. and Iype, P.
Influence of Cultures on Cell Morphology/Tyrosine Aminotransferase Levels ,
Exp Cell Res
 131 , 363 , 1981
Cells
Epithelial
Enzymes
Trypsin : 0.05%
Medium
HBSS, CMF
Liver
Rat
Rat, SD
Reese, J. and Byard, J.
Isolation And Culture of Adult Hepatocytes from Liver Biopsies ,
In Vitro
 17 , 935 , 1981
Cells
Hepatocytes
Enzymes
Collagenase : 90 u/ml
Medium
CF EGTA perfusate
Liver
Rat
Rat, Zucker, obese
Goldstein, A. , Palmer, J. and Johnson, P.
Primary Cultures of Fetal Hepatocytes from the Genetically Obese Zucker Rat: Protein Synthesis ,
In Vitro
 17 , 651 , 1981
Cells
Hepatocytes
Enzymes
Medium
Dulbecco-Vogt arginine free Eagle's
Liver
Rat
Rat, Wistar, male, 180-210 g
De Gerlache, J. , Lans, M. , Taper, H. and Roberfroid, M.
Separate Isolation of Cells from Nodules and Surrounding Parenchyma of the Same Precancerous Rat Liver: Biochemical and Cytochemical Characterization ,
Toxicology
 18 , 225 , 1980
Cells
Hepatocytes
Enzymes
Hyaluronidase : 0.02%
Medium
Kreb's buffer
Liver
Rat
Rat, Wistar, female, fetus
Gugen-Guillouzo, C. , Tichonicky, L. , Szajnert, M. and Kruh, J.
Changes in Some Chromatin and Cytoplastic Enzymes of Perinatal Rat Hepatocytes ,
In Vitro
 16 , 1 , 1980
Cells
Hepatocytes
Enzymes
Collagenase : 0.025%
Medium
HEPES buffer
Liver
Rat
Rat, SD, 7-10 day
Acosta, D. , Anuforo, D. and Smith, R
Preparation of Primary Monolayer Cultures of Postnatal Rat Liver Cells ,
J Tiss Cul Meth
 6 , 35 , 1980
Cells
Liver
Enzymes
Collagenase : 0.05%
Medium
HBSS modified (see reference)
Liver
Rat
Rat, SD, male, 250-300 g
Yamada, S. , Otto, P. , Kennedy, D. and Whayne, T.
The Effects of Dexamethasone on Metabolic Activity of Hepatocytes in Primary Monolayer Culture ,
In Vitro
 16 , 559 , 1980
Cells
Parenchymal
Enzymes
Medium
Kreb's Henseleit bicarbonate buffer
Liver
Rat
Rat, Wistar, male, 200-250 g
Poli, G. , Gravela, E. , Albano, E. and Dianzani, M.
Studies on Fatty Liver with Isolated Hepatocytes: II., The Action of Carbon Tetrachloride on Lipid Peroxidation, Protein and Triglyceride Synthesis and Secretion ,
Exp Mol Pathol
 30 , 116 , 1979
Cells
Hepatocytes
Enzymes
Hyaluronidase : 460 u/ml
Medium
Saline
Liver
Rat
Rat, SD, female, 100-150 g
Davis, R. , Engelhorn, S. , Pangburn, S. , Weinstein, D. and Steinberg, D.
Very Low Density Lipoprotein Synthesis and Secretion by Cultured Rat Hepatocytes ,
J Biol Chem
 254 (6) , 2010 , 1979
Cells
Hepatocytes
Enzymes
Medium
DMEM
(see reference)
Liver
Rat
Rat
(also chicken)
Weigel, P. , Schnaar, R. , Kuhlenschmidt, M. , Schmell, E. , Lee, R. , Lee, Y. and Roseman, S.
Adhesion of Hepatocytes to Immobilized Sugars ,
J Biol Chem
 254 (21) , 10830 , 1979
Cells
Hepatocytes
Enzymes
Medium
HEPES
Liver
Rat
Rat , Wistar, male, 250-300 g
Gravela, E. , Poli, G. , Albano, E. and Dianzani, M.
Studies of Fatty Liver with Isolated Hepatocytes ,
Exp Mol Pathol
 27 , 339 , 1977
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
Hank's solution, CF
Liver
Rat
Rat, SD, male, 200 g
Rubin, K. , Kjellen, L. and Oslashbrink, B.
Intercellular Adhesion between Juvenile Liver Cells.. A Method to Measure the Formation of Stable Lateral Contacts Between Cells Attached to a Collagen Gel ,
Exp Cell Res
 109 , 413 , 1977
Cells
Hepatocytes
Enzymes
Collagenase Type 1 : 100 u/ml
Medium
Buffers 1 & 2
(see reference)
Liver
Rat
Rat (WAG/RIJ), female, 24, 30, and 37 months
VanBezodijen, C. , Grell, T. and Knook, D.
Effect of Age on Protein Synthesis by Isolated Liver Parenchymal Cells ,
Mech Ageing Dev
 6 , 293 , 1977
Cells
Parenchymal
Enzymes
Collagenase Type 1 : 0.05% - 0.06%
Medium
HEPES buffer
Liver
Rat
Rat, Fisher, adult, male
Williams, G. , Bermudez, E. and Scaramuzzino, D.
Rat Hepatocytes Primary Cell Cultures III. Improved Dissociation and Attachment Techniques and the Enchancement of Survival by Culture Medium ,
In Vitro
 13 (12) , 809 , 1977
Cells
Hepatocytes
Enzymes
Collagenase Type 1 : 100 u/ml
Medium
HBSS
(see reference)
Liver
Rat
Rat, Fischer, male, adult, 170-265 g
Laishes, B. and Williams, G.
Conditions Affecting Primary Cell Cultures of Functional Adult Rat Hepatocytes. I.. The Effect of Insulin ,
In Vitro
 12 , 521 , 1976
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%-0.10%
Medium
Williams E
Liver
Rat
Rat, SD, male, 200-250 g
Witters, L. , Alberico, L. and Acruch, J.
Insulin Regulation of Glycogen Synthase in the Isolated Rat Hepatocyte ,
Biochem Biophys Res Commun
 69 (4) , 997 , 1976
Cells
Hepatocytes
Enzymes
Medium
MEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.