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Adipose/Fat
Avian
Scherpelz, K. , Lu, J.,Tycko, R. and Meredith, S.
Preparation of Amyloid Fibrils Seeded from Brain and Meninges ,
Methods Mol Biol
 1345 , 299 , 2016
Cells
Amyloid Fibrils
Enzymes
Medium
50mM Tris
Adipose/Fat
Avian
Scherpelz, K. , Lu, J.,Tycko, R. and Meredith, S.
Preparation of Amyloid Fibrils Seeded from Brain and Meninges ,
Methods Mol Biol
 1345 , 299 , 2016
Cells
Amyloid Fibrils
Enzymes
Medium
50mM Tris
Adipose/Fat
Bear
Bear, Ursus arctos
Gehring, J. , Rigano, K. , Evans, H. , Nelson, O. , Robbins, C. and Jansen, H.
A Protocol for the Isolation and Cultivation of Brown Bear (Ursus arctos) Adipocytes. ,
Cytotechnology
 68 , 2177-91 , 2016
Cells
Adipocytes
Enzymes
Medium
HBSS
Adipose/Fat
Bovine
Bovine
Yang, Y. and Baldwin, R.
Preparation and Metabolism of Isolated Cells from Bovine Adipose Tissue ,
J Dairy Sci
 56 , 350 , 1973
Cells
Adipocytes
Enzymes
Collagenase Type 1 : 40 u/ml
Medium
Krebs-Ringer bicarbonate
Adipose/Fat
Canine
23-33 kg
Williams, S. , Kosnik, P. , Kleinert, L. , Vossman, E. , Lye, K. and Shine, M.
Adipose Stromal Vascular Fraction Cells Isolated Using an Automated Point of Care System Improve the Patency of Expanded Polytetrafluoroethylene Vascular Grafts. ,
Tissue Eng Part A
 19 , 1295-302 , 2013
Cells
Stromal vascular
Enzymes
Collagenase : see reference
Medium
Media 199E
File
Adipose/Fat
Canine
Canine
Basu, J. , Genheimer, C. , Sangha, N. , Quinlan, S. , Guthrie, K. , Kelley, R. , Ilagan, R. , Jain, D. , Bertram, T. and Ludlow, J.
Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney. ,
Lipids Health Dis
 10 , 171 , 2011
Cells
Renal adipose derived cells
Enzymes
Medium
DMEM
Adipose/Fat
Canine
Canine, 20-25 kg
Fischer, L. , McIlhenny, S. , Tulenko, T. , Golesorkhi, N. , Zhang, P. , Larson, R. , Lombardi, J. , Shapiro, I. and DiMuzio, P.
Endothelial Differentiation of Adipose-Derived Stem Cells: Effects of Endothelial Cell Growth Supplement and Shear Force. ,
J Surg Res
 152 , 157 , 2009
Cells
Adipose stem cells
Enzymes
Collagenase : see reference
Medium
Media-199
File
Adipose/Fat
Canine
Canine
DiGirolamo, M. , Mendlinger, S. and Fertig, J.W.
A Simple Method to Determine Fat Cell Size and Number in Four Mammalian Species ,
Am J Physiol
 221 , 850 , 1971
Cells
White fat
Enzymes
Collagenase : 0.05%
Medium
Kreb's Ringer bicarbonate buffer
Adipose/Fat
Equine
Equine
Vidal, M. , Robinson, S. , Lopez, M. , Paulsen, D. , Borkhsenious, O. , Johnson, J. , Moore, R. and Gimble, J.
Comparison of Chondrogenic Potential in Equine Mesenchymal Stromal Cells Derived from Adipose Tissue and Bone Marrow. ,
Vet Surg
 37 , 713 , 2008
Cells
Adipose derived stem cells
Enzymes
Medium
PBS
Adipose/Fat
Equine
Equine, 1-5 year
Vidal, M. , Kilroy, G. , Lopez, M. , Johnson, J. , Moore, R. and Gimble, J.
Characterization of Equine Adipose Tissue-Derived Stromal Cells: Adipogenic and Osteogenic Capacity and Comparison with Bone Marrow-Derived Mesenchymal Stromal Cells. ,
Vet Surg
 36 , 613 , 2007
Cells
Adipose derived stem cells
Enzymes
Medium
PBS
Adipose/Fat
Fish
Fish, Atlantic salmon
Todorcevic, M. , Vegusdal, A. , Gjoen, T. , Sundvold, H. , Torstensen, B. , Kjaer, M. and Ruyter, B.
Changes in Fatty Acids Metabolism During Differentiation of Atlantic Salmon Preadipocytes; Effects of n-3 and n-9 Fatty Acids. ,
Biochim Biophys Acta
 1781 , 326 , 2008
Cells
Preadipocytes
Enzymes
Medium
HBSS
Adipose/Fat
Gerbil
Gerbil of unknown age
(also rat, hamster, rabbit, lamb, guinea-pig)
Nedergaard, J. and Lindberg, O.
The Brown Fat Cell ,
Int Rev Cytol
 74 , 187 , 1982
Cells
Brown fat
Enzymes
Medium
Bicarbonate buffer
Adipose/Fat
Guinea-Pig
Guinea-pig, adult
(also rat, hamster, gerbil, rabbit, lamb)
Nedergaard, J. and Lindberg, O.
The Brown Fat Cell ,
Int Rev Cytol
 74 , 187 , 1982
Cells
Brown fat
Enzymes
Medium
Bicarbonate buffer
Adipose/Fat
Hamster
Hamster, adult
(also rat, gerbil, rabbit, lamb, guinea-pig)
Nedergaard, J. and Lindberg, O.
The Brown Fat Cell ,
Int Rev Cytol
 74 , 187 , 1982
Cells
Brown fat
Enzymes
Medium
Bicarbonate buffer
Adipose/Fat
Hamster
Hamster, 5 week-12 month
DiGirolamo, M. , Mendlinger, S. and Fertig, J.W.
A Simple Method to Determine Fat Cell Size and Number in Four Mammalian Species ,
Am J Physiol
 221 , 850 , 1971
Cells
White fat
Enzymes
Collagenase : 0.05%
Medium
Kreb's Ringer bicarbonate buffer
Adipose/Fat
Human
Human
Alstrup, T. , Eijken, M. , Bohn, A. , Moller, B. and Damsgaard, T.
Isolation of Adipose Tissue-Derived Stem Cells: Enzymatic Digestion in Combination with Mechanical Distortion to Increase Adipose Tissue-Derived Stem Cell Yield from Human Aspirated Fat. ,
Curr Protoc Stem Cell Biol
 48 , e68 , 2019
Cells
Adipose derived stem
Enzymes
Collagenase Type 4 : 150-300 u/ml
Medium
RPMI-1640
File
Adipose/Fat
Human
Human
Tsurumachi, N. , Akita, D. , Kano, K. , Matsumoto, T. , Toriumi, T. , Kazama, T. , Oki, Y. , Saito-Tamura, Y. , Tonogi, M. , Shimizu, N. and Honda, M.
Effect of Collagenase Concentration on The Isolation of Small Adipocytes from Human Buccal Fat Pad. ,
J Oral Sci
 , 2018
Cells
Adipocytes
Enzymes
Collagenase Type 2 : 0.01-0.5%
Medium
DMEM
File
Adipose/Fat
Human
Human
Wahl, S. , Drong, A. , Lehne, B. , Loh, M. , Scott, K. and Kunze, S. et al.
Epigenome-wide Association Study of Body Mass Index, and the Adverse Outcomes of Adiposity ,
Nature
 541 , 81 , 2017
Cells
Adipocytes
Enzymes
Medium
HBSS
File
Adipose/Fat
Human
Human
Munir, H. , Ward, L. , Sheriff, L. , Kemble, S. , Nayar, S. , Barone, F. , Nash, G. and McGettrick, H.
Adipogenic Differentiation of Mesenchymal Stem Cells Alters Their Immunomodulatory Properties in a Tissue-Specific Manner. ,
Stem Cells
 35 , 1636-1646 , 2017
Cells
Mesenchymal stem
Enzymes
Medium
DMEM/F-12
File
Adipose/Fat
Human
Human
Clark, K. , Fierro, F. , Ko, E. , Walker, N. , Arzi, B. , Tepper, C. , Dahlenburg, H. , Cicchetto, A. , Koi, A. , Marsh, L. , Murphy, W. , Fazel, N. and Borjesson, D.
Human and Feline Adipose-Derived Mesenchymal Stem Cells Have Comparable Phenotype, Immunomodulatory Functions, and Transcriptome. ,
Stem Cell Res Ther
 8 , 69 , 2017
Cells
Adipose derived mesenchymal stem
Enzymes
Medium
PBS
File
Adipose/Fat
Human
Human
Duscher, D. , Maan, Z. , Luan, A. , Aitzetmuller, M. , Brett, E. , Atashroo, D. , Whittam, A. , Hu, M. , Walmsley, G. , Houschyar, K. , Schilling, A. , Machens, H. , Gurtner, G. , Longaker, M. and Wan, D.
Ultrasound-Assisted Liposuction Provides a Source for Functional Adipose-Derived Stromal Cells. ,
Cytotherapy
 19 , 1491-1500 , 2017
Cells
Stromal
Enzymes
Medium
DMEM
File
Adipose/Fat
Human
Human
Lin, Y. , Marin-Argany, M. , Dick, C. , Redhage, K. , Blancas-Mejia, L. , Bulur, P. , Butler, G. , Deeds, M. , Madden, B. , Williams, A. , Wall, J. , Dietz, A. and Ramirez-Alvarado, M.
Mesenchymal Stromal Cells Protect Human Cardiomyocytes from Amyloid Fibril Damage ,
Cytotherapy
 19 , 1426-1437 , 2017
Cells
Mesenchymal stromal
Enzymes
Medium
PBS
File
Adipose/Fat
Human
Human
Hu, J. , Cao, Y. , Xie, Y. , Wang, H. , Fan, Z. , Wang, J. , Zhang, C. , Wang, J. and Wu, C. aand Wang, S.
Periodontal Regeneration in Swine After Cell Injection and Cell Sheet Transplantation of Human Dental Pulp Stem Cells Following Good Manufacturing Practice. ,
Stem Cell Res Ther
 7 , 130 , 2016
Cells
Dental pulp stem
Enzymes
Medium
HBSS
File
Adipose/Fat
Human
Human
Szoke, K. , Reinisch, A. , Ostrup, E. , Reinholt, F. and Brinchmann, J.
Autologous Cell Sources in Therapeutic Vasculogenesis: In Vitro and In Vivo Comparison of Endothelial Colony-Forming Cells from Peripheral Blood and Endothelial Cells Isolated from Adipose Tissue. ,
Cytotherapy
 18 , 242-52 , 2016
Cells
Adipose derived endothelial
Enzymes
Medium
DMEM
File
Adipose/Fat
Human
Human
Cheng, N. , Hsieh, T. , Lai, H. and Young, T.
High Glucose-Induced Reactive Oxygen Species Generation Promotes Stemness in Human Adipose-Derived Stem Cells ,
Cytotherapy
 18 , 371-83 , 2016
Cells
Adipose stem
Enzymes
Medium
DMEM/F12
File
Adipose/Fat
Human
Human
Choudhery,M, Badowski, M, Muise, A. and Harris, D
Effect of Mild Heat Stress on the Proliferative and Differentiative Ability of Human Mesenchymal Stromal Cells. ,
Cytotherapy
 17 , 359-68 , 2015
Cells
Mesenchymal stromal
Enzymes
Medium
PBS
File
Adipose/Fat
Human
Human
Satish, L. , Krill-Burger, J. , Gallo, P. , Etages, S. , Liu, F. , Philips, B. , Ravuri, S. , Marra, K. , LaFramboise, W. , Kathju, S. and Rubin, J.
Expression Analysis of Human Adipose-Derived Stem Cells During In Vitro Differentiation to an Adipocyte Lineage. ,
BMC Med Genomics
 8 , 41 , 2015
Cells
Adipose derived stem cells
Enzymes
Medium
HBSS
File
Adipose/Fat
Human
Human, female
Kinoshita, K. , Kuno, S. , Ishimine, H. , Aoi, N. , Mineda, K. , Kato, H. , Doi, K. , Kanayama, K. , Feng, J. , Mashiko, T. , Kurisaki, A. and Yoshimura, K.
Therapeutic Potential of Adipose-Derived SSEA-3-Positive Muse Cells for Treating Diabetic Skin Ulcers. ,
Stem Cells Transl Med
 4 , 146 , 2015
Cells
Adipose stromal
Enzymes
Medium
DMEM
File
Adipose/Fat
Human
Human
Esteve, D. , Boulet, N. , Volat, F. , Zakaroff-Girard, A. , Ledoux, S. , Coupaye, M. , Decaunes, P. , Belles, C. , Gaits-Iacovoni, F. , Iacovoni, J. , Remaury, A. , Castel, B. , Ferrara, P. , Heymes, C. and Lafonta, M. Boloumie, J.
Human White and Brite Adipogenesis is Supported by MSCA1 and is impaired by Immune Cells. ,
Stem Cells
 33 , 1277-91 , 2015
Cells
Adipose stroma-vascular
Enzymes
Neutral Protease : 2.4 u/ml
Collagenase : 250 u/ml
Medium
PBS
File
Adipose/Fat
Human
Human
Seaman, S. , Tannan, S. , Cao, Y. , Peirce, S. and Lin, K.
Differential Effects of Processing Time and Duration of Collagenase Digestion on Human and Murine Fat Grafts. ,
Plast Reconstr Surg
 136 , 189e-199e , 2015
Cells
Adipocytes
Enzymes
Medium
PBS
File
Adipose/Fat
Human
Human, adult
Al-Saqi, S. , Saliem, M. , Asikainen, S. , Quezada, H. , Ekblad, A. , Hovatta, O. , Le Blanc, K. , Jonasson, A. and Gotherstrom, C.
Defined Serum-Free Media for In Vitro Expansion of Adipose-Derived Mesenchymal Stem Cells. ,
Cytotherapy
 16 , 915 , 2014
Cells
Adipose derived mesenchymal stem
Enzymes
Medium
see reference
Adipose/Fat
Human
Human, adult
Koellensperger, E. , Bollinger, N. , Dexheimer, V. , Gramley, F. , Germann, G. and Leimer, U.
Choosing the Right Type of Serum for Different Applications of Human Adipose Tissue-Derived Stem Cells: Influence on Proliferation and Differentiation Abilities. ,
Cytotherapy
 16 , 789 , 2014
Cells
Adipose derived stem
Enzymes
Medium
DMEM
Adipose/Fat
Human
Human
Najar, M, Rodrigues, R, Buyl, K, Branson, S, Vanhaecke, T, Lagneaux, L, Rogiers, V. and De Kock, J
Proliferative and Phenotypical Characteristics of Human Adipose Tissue-Derived Stem Cells: Comparison of Ficoll Gradient Centrifugation and Red Blood Cell Lysis Buffer Treatment Purification Methods. ,
Cytotherapy
 16 , 1220-8 , 2014
Cells
Mesenchymal stromal
Enzymes
Medium
PBS
File
Adipose/Fat
Human
Human
Doi, K. , Tanaka, S. , Iida, H. , Eto, H. , Kato, H. , Aoi, N. , Kuno, S. , Hirohi, T. and Yoshimura, K.
Stromal Vascular Fraction Isolated from Lipo-Aspirates Using an Automated Processing System: Bench and Bed Analysis. ,
J Tissue Eng Regen Med
 7 , 864 , 2013
Cells
Stromal vascular fraction
Enzymes
Medium
PBS
File
Adipose/Fat
Human
Human
Carvalho, P. , Gimble, J. , Dias, I. , Gomes, M. and Reis, R.
Xenofree Enzymatic Products for the Isolation of Human Adipose-Derived Stromal/Stem Cells. ,
Tiss Eng
 19 , 473-8 , 2013
Cells
Adipose stromal stem
Enzymes
Medium
DMEM/Hams F-12
File
Adipose/Fat
Human
Human
Cervelli, V. , Scioli, M. , Gentile, P. , Doldo, E. , Bonanno, E. , Spagnoli, L. and Orlandi, A.
Platelet-Rich Plasma Greatly Potentiates Insulin-Induced Adipogenic Differentiation of Human Adipose-Derived Stem Cells Through a Serine/Threonine Kinase Akt-dependent Mechanism and Promotes Clinical Fat Graft Maintenance. ,
Stem Cells Transl Med
 1 , 206-20 , 2012
Cells
Adipose-derived stem
Enzymes
Medium
PBS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.