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Tissue References Search Tool

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Adipose/Fat
Human
Human
Cervelli, V. , Scioli, M. , Gentile, P. , Doldo, E. , Bonanno, E. , Spagnoli, L. and Orlandi, A.
Platelet-Rich Plasma Greatly Potentiates Insulin-Induced Adipogenic Differentiation of Human Adipose-Derived Stem Cells Through a Serine/Threonine Kinase Akt-dependent Mechanism and Promotes Clinical Fat Graft Maintenance. ,
Stem Cells Transl Med
 1 , 206-20 , 2012
Cells
Adipose-derived stem
Enzymes
Medium
PBS
Adipose/Fat
Human
Human
Gentile, P. , Orlandi, A. , Scioli, M. , Di Pasquali, C. , Bocchini, I. and Cervelli, V.
Concise Review: Adipose-Derived Stromal Vascular Fraction Cells and Platelet-Rich Plasma: Basic and Clinical Implications for Tissue Engineering Therapies in Regenerative Surgery. ,
Stem Cells Transl Med
 1 , 230-6 , 2012
Cells
Adipose derived stromal vascular
Enzymes
Medium
PBS
Adipose/Fat
Human
Human, adult
Hagman, D. , Kuzma, J. , Larson, I. , Foster-Schubert, K. , Kuan, L. , Cignarella, A. , Geamanu, E. , Makar, K. , Gottlieb, J. and Kratz, M.
Characterizing and Quantifying Leukocyte Populations in Human Adipose Tissue: Impact of Enzymatic Tissue Processing. ,
J Immunol Methods
 386 , 50 , 2012
Cells
Stromovascular
Enzymes
Medium
PBS with BSA
File
11050.pdf (955.53 KB)
Adipose/Fat
Human
Human
Wu, I. , Nahas, Z. , Kimmerling, K. , Rosson, G. and Elisseeff, J.
An Injectable Adipose Matrix for Soft-Tissue Reconstruction. ,
Plast Reconstr Surg
 129 , 1247 , 2012
Cells
Adipose derived stem
Enzymes
Medium
DMEM/F-12
File
11085.pdf (2.66 MB)
Adipose/Fat
Human
Human
Gentile, P. , Orlandi, A. , Scioli, M. , Di Pasquali, C. , Bocchini, I. , Curcio, C. , Floris, M. , Fiaschetti, V. , Floris, R. and Cervell, V.
A Comparative Translational Study: The Combined Use of Enhanced Stromal Vascular Fraction and Platelet-Rich Plasma Improves Fat Grafting Maintenance in Breast Reconstruction. ,
Stem Cells Transl Med
 1 , 341-51 , 2012
Cells
Stromal vascular, adipocytes
Enzymes
Medium
DMEM
Adipose/Fat
Human
Human
Naaijkens, B. , Niessen, H. , Prins, H. , Krijnen, P. , Kokhuis, T. , de Jong, N,, van Hinsbergh, V. , Kamp, O. , Helder, M. , Musters, R. , van Dijk, A. and Juffermans, L.
Human Platelet Lysate as a Fetal Bovine Serum Substitute Improves Human Adipose-Derived Stromal Cell Culture for Future Cardiac Repair Applications. ,
Cell Tissue Res
 348 , 119 , 2012
Cells
Adipose derived stromal
Enzymes
Medium
PBS
File
11076.pdf (650.05 KB)
Adipose/Fat
Human
Human
James, A. , Zara, J. , Corselli, M. , Askarinam, A. , Zhou, A. , Hourfar, A. , Nguyen, A. , Megerdichian, S. , Asatrian, G. , Pang, S. , Stoker, D. , Zhang, X. , Wu, B. , Ting, K. , Peault, B. and Soo, C.
An Abundant Perivascular Source of Stem Cells for Bone Tissue Engineering. ,
Stem Cells Transl Med
 1 , 673 , 2012
Cells
Perivascular stem
Enzymes
Medium
DMEM
Adipose/Fat
Human
Human, male 40-60 years
Blasi, A. , Martino, C. , Balducci, L. , Saldarelli, M. , Soleti, A. , Navone, S. , Canzi, L. , Cristini, S. , Invernici, G. , Parati, E. and Alessandri, G.
Dermal Fibroblasts Display Similar Phenotypic and Differentiation Capacity to Fat-Derived Mesenchymal Stem Cells, but Differ in Anti-Inflammatory and Angiogenic Potential ,
Vasc Cell
 3 , 5 , 2011
Cells
Adipose derived stem cells
Enzymes
Collagenase : 0.25%
Medium
PBS
Adipose/Fat
Human
Human
Basu, J. , Genheimer, C. , Sangha, N. , Quinlan, S. , Guthrie, K. , Kelley, R. , Ilagan, R. , Jain, D. , Bertram, T. and Ludlow, J.
Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney. ,
Lipids Health Dis
 10 , 171 , 2011
Cells
Renal adipose derived cells
Enzymes
Medium
DMEM
Adipose/Fat
Human
Human
Yang, X. , He, X. , He, J. , Zhang, L. , Su, X. , Dong, Z. , Xu, Y. , Li, Y. and Li, YL.
High Efficient Isolation and Systematic Identification of Human Adipose-Derived Mesenchymal Stem Cells. ,
J Biomed Sci
 18 , 59 , 2011
Cells
Adipose derived mesenchymal
Enzymes
Medium
L-DMEM
File
10930.pdf (7.71 MB)
Adipose/Fat
Human
Human
Yu, G. Floyd, ZE. , Wu, X. , Halvorsen, Y. and Gimble, J.
Isolation of Human Adipose-Derived Stem Cells from Lipoaspirates. ,
Methods Mol Biol
 702 , 17-27 , 2011
Cells
Adipose derived stem
Enzymes
Medium
DMEM/Ham's F-12
Adipose/Fat
Human
Human
Basu, S. , Haghiac, M. , Surace, P. , Challier, J. , Guerre-Millo, M. , Singh, K. , Waters, T. , Minium, J. , Presley, L. , Catalano, P. and Hauguel-de Mouzon, S.
Pregravid Obesity Associates with Increased Maternal Endotoxemia and Metabolic Inflammation. ,
Obesity
 19 , 476 , 2011
Cells
Adipocytes
Enzymes
Medium
RPMI
Adipose/Fat
Human
Human
Peters, R. , Wolf, M. , van den Broek, M. , Nuvolone, M. , Dannenmann, S. , Stieger, B. , Rapold, R. , Konrad, D. , Rubin, A. , Bertino, J. , Aguzzi, A. , Heikenwalder, M. and Knuth, A.
Efficient Generation of Multipotent Mesenchymal Stem Cells from Umbilical Cord Blood in Stroma-Free Liquid Culture. ,
PLoS ONE
 5 , e15689 , 2010
Cells
Adipocytes
Enzymes
Medium
KRB
Adipose/Fat
Human
Human
Tan, H. , DeFail, A. , Rubin, J. , Chu, C. and Marra, K.
Novel Multiarm PEG-Based Hydrogels for Tissue Engineering. ,
J Biomed Mater Res
 92 , 979 , 2010
Cells
Adipose tissue-derived stem
Enzymes
Medium
DMEM/F12
File
10925.pdf (704.73 KB)
Adipose/Fat
Human
Human
Zimmerlin, L. , Donnenberg, V. , Pfeifer, M. , Meyer, EM, Peault, B. , Rubin, JP. and Donnenberg, A.
Stromal Vascular Progenitors in Adult Human Adipose Tissue. ,
Cytometry
 77 , 22-30 , 2010
Cells
Stromal vascular
Enzymes
Medium
HBSS
File
11408.pdf (880.91 KB)
Adipose/Fat
Human
Human
Yu, G. , Wu, X. , Dietrich, M. , Polk, P. , Scott, LK, Ptitsyn, A. and Gimble, J.
Yield and Characterization of Subcutaneous Human Adipose-Derived Stem Cells by Flow Cytometric and Adipogenic mRNA Analyzes. ,
Cytotherapy
 12 , 538 , 2010
Cells
Adipose stromal
Enzymes
Medium
DMEM/Hams F-12
File
11463.pdf (7.32 MB)
Adipose/Fat
Human
Human
Suga, H. , Eto, H. , Shigeura, T. , Inoue, K. , Aoi, N. , Kato, H. , Nishimura, S. , Manabe, I. , Gonda, K. and Yoshimura, K.
IFATS Collection: Fibroblast Growth Factor-2-Induced Hepatocyte Growth Factor Secretion by Adipose-Derived Stromal Cells Inhibits Postinjury Fibrogenesis Through a c-Jun N-Terminal Kinase-Dependent Mechanism. ,
Stem Cells
 27 , 238 , 2009
Cells
Adipocytes
Enzymes
Collagenase : 0.075%
Medium
DMEM
File
10872.pdf (1.35 MB)
Adipose/Fat
Human
Human
Cai, L. , Johnstone, B. , Cook, T. , Tan, J. , Fishbein, M. , Chen, P. and March, K.
IFATS Collection: Human Adipose Tissue-Derived Stem Cells Induce Angiogenesis and Nerve Sprouting Following Myocardial Infarction, in Conjunction with Potent Preservation of Cardiac Function. ,
Stem Cells
 27 , 230 , 2009
Cells
Adipose stromal
Enzymes
Medium
DMEM
Adipose/Fat
Human
Human
Tan, H. , Ramirez, C. , Miljkovic, N. , Li, H. , Rubin, J. and Marra, K.
Thermosensitive Injectable Hyaluronic Acid Hydrogel for Adipose Tissue Engineering. ,
Biomaterials
 30 , 6844 , 2009
Cells
Adipose tissue-derived stem
Enzymes
Medium
DMEM/F12
File
10924.pdf (4.55 MB)
Adipose/Fat
Human
Human
Tandon, N. , Goh, B. , Marsano, A. , Chao, P. , Montouri-Sorrentino, C. , Gimble, J. and Vunjak-Novakovic, G.
Alignment and Elongation of Human Adipose-Derived Stem Cells in Response to Direct-Current Electrical Stimulation. ,
Conf Proc IEEE Eng Med Biol Soc
 2009 , 6517-21 , 2009
Cells
Adipose derived stem cells
Enzymes
Medium
DMEM
Adipose/Fat
Human
Human
Pilgaard, L. , Lund, P. , Rasmussen, J. , Fink, T.. and Zachar, V.
Comparative Analysis of Highly Defined Proteases for the Isolation of Adipose Tissue-Derived Stem Cells. ,
Regen Med
 3 , 705-15 , 2008
Cells
Stem
Enzymes
Collagenase : 280 u/ml
Medium
D-PBS
File
11261.pdf (413.9 KB)
Adipose/Fat
Human
Human
Traktuev, D. , Merfeld-Clauss, S. , Li, J. , Kolonin, M. , Arap, W. , Pasqualini, R. , Johnstone, B. and March, K.
A Population of Multipotent CD34-Positive Adipose Stromal Cells Share Pericyte and Mesenchymal Surface Markers, Reside in a Periendothelial Location, and Stabilize Endothelial Networks ,
Circ Res
 102 , 77 , 2008
Cells
Adipose derived stromal cells
Enzymes
Collagenase Type 1 : see reference
Medium
DMEM
Adipose/Fat
Human
Human
Minana, M. , Carbonell-Uberos, F. , Mirabet, V. , Marin, S. and Encabo, A.
IFATS Collection: Identification of Hemangioblasts in the Adult Human Adipose Tissue. ,
Stem Cells
 26 , 2696 , 2008
Cells
Stromal
Enzymes
Medium
PBS
File
10876.pdf (3.45 MB)
Adipose/Fat
Human
Human
Nie, J. , Chang, B. , Traktuev, D. , Sun, J. , March, K. , Chan, L. , Sage, H. , Pasqualini, R. , Arap, W. and Kolonin, M.
IFATS Collection: Combinatorial Peptides Identify alpha-5 beta-1 Integrin as a Receptor for the Matricellular Protein SPARC on Adipose Stromal Cells. ,
Stem Cells
 26 , 2735 , 2008
Cells
Stromal
Enzymes
Medium
DMEM
Adipose/Fat
Human
Human
Schaffler A, Buchler C.
Concise review: adipose tissue-derived stromal cells--basic and clinical implications for novel cell-based therapies ,
Stem Cells
 25 , 818-27 , 2007
Cells
Adipose derived stromal cells
Enzymes
Medium
DMEM
Adipose/Fat
Human
Human
Bujalska Iwona J, Durrani Omar M, Abbott Joseph, Onyimba Claire U, Khosla Pamela, Moosavi Areeb H, Reuser Tristan T Q, Stewart Paul M, Tomlinson Jeremy W, Walker Elizabeth A, Rauz Saaeha.
Characterisation of 11beta-hydroxysteroid dehydrogenase 1 in human orbital adipose tissue: a comparison with subcutaneous and omental fat ,
J Endocrinol
 192 , 279-88 , 2007
Cells
Adipocytes
Enzymes
Medium
HBSS
Adipose/Fat
Human
Human
Kilroy, G. , Foster, S. , Wu, X. , Ruiz, J. , Sherwood, S. , Heifetz, A. , Ludlow, J. , Stricker, D. , Potiny, S. , Green, P. , Halvorsen, Y. , Cheatham, B. , Storms, R. and Gimble, J.
Cytokine Profile of Human Adipose-Derived Stem Cells: Expression of Angiogenic, Hematopoietic, and Pro-Inflammatory Factors. ,
J Cell Physiol
 212 , 702-9 , 2007
Cells
Adipose derived stem cells
Enzymes
Medium
DMEM/F12
Adipose/Fat
Human
Human
Jeon Eun Su, Song Hae Young, Kim Mi Ra, Moon Hyun Jung, Bae Yong Chan, Jung Jin Sup, Kim Jae Ho.
Sphingosylphosphorylcholine induces proliferation of human adipose tissue-derived mesenchymal stem cells via activation of JNK ,
J Lipid Res
 47 , 653-64 , 2006
Cells
Mesenchymal stem
Enzymes
Medium
HBSS
Adipose/Fat
Human
Human
Koellensperger, E. , Von Heimburg, D. , Markowicz, M. and Pallua, N.
Human Serum from Platelet-Poor Plasma for the Culture of Primary Human Preadipocytes. ,
Stem Cells
 24 , 1218-25 , 2006
Cells
Preadipocytes
Enzymes
Collagenase Type 1 : 196 u/ml
Medium
M199
Adipose/Fat
Human
Human
Boquest Andrew C, Shahdadfar Aboulghassem, Fronsdal Katrine, Sigurjonsson Olafur, Tunheim Siv H, Collas Philippe, Brinchmann Jan E.
Isolation and transcription profiling of purified uncultured human stromal stem cells: alteration of gene expression after in vitro cell culture ,
Mol Biol Cell
 16 , 1131-41 , 2005
Cells
Adipocytes, stromal vascular
Enzymes
Collagenase : 0.2%
Medium
HBSS
Adipose/Fat
Human
Human
Rodriguez Anne-Marie, Pisani Didier, Dechesne Claude A, Turc-Carel Claude, Kurzenne Jean-Yves, Wdziekonski Brigitte, Villageois Albert, Bagnis Claude, Breittmayer Jean-Philippe, Groux Herve, Ailhaud Gerard, Dani Christian.
Transplantation of a multipotent cell population from human adipose tissue induces dystrophin expression in the immunocompetent mdx mouse ,
J Exp Med
 201 , 1397-405 , 2005
Cells
Multipotent adipose derived stem
Enzymes
Collagenase : 0.2%
Medium
DMEM
Adipose/Fat
Human
Human
Devireddy, R. , Thirumala, S. and Gimble, J.
Cellular Response of Adipose Derived Passage-4 Adult Stem Cells to Freezing Stress. ,
J Biomech Eng
 127 , 1081 , 2005
Cells
Adult stem cells
Enzymes
Medium
PBS
Adipose/Fat
Human
Human
Planat-Benard Valerie, Silvestre Jean-Sebastien, Cousin Beatrice, Andre Mireille, Nibbelink Maryse, Tamarat Radia, Clergue Michel, Manneville Carole, Saillan-Barreau Corinne, Duriez Micheline, Tedgui Alain, Levy Bernard, Penicaud Luc, Casteilla Louis.
Plasticity of human adipose lineage cells toward endothelial cells: physiological and therapeutic perspectives ,
Circulation
 109 , 656-63 , 2004
Cells
Stromal vascular, adipocytes
Enzymes
Collagenase : 0.2%
Medium
DMEM/F12
Adipose/Fat
Human
Human
Miranville A, Heeschen C, Sengenes C, Curat CA, Busse R, Bouloumie A.
Improvement of postnatal neovascularization by human adipose tissue-derived stem cells ,
Circulation
 110 , 349-55 , 2004
Cells
Stromal vascular, adipocytes, stem
Enzymes
Collagenase : 300 u/ml
Medium
PBS
Adipose/Fat
Human
Human, adult, female
Quickler, M. , Sinha, B. , Tomlinson, J. , Bujalska, I. , Stewart, P. and Arlt, W.
Androgen generation in adipose tissue in women with simple obesity--a site-specific role for 17beta-hydroxysteroid dehydrogenase type 5 ,
J Endocrinol
 183 , 331 , 2004
Cells
Preadipocytes
Enzymes
Medium
DMEM/F-12
Adipose/Fat
Human
Human, adult
Fain JN, Madan AK, Hiler ML, Cheema P. and Bahouth SW.
Comparison of the release of adipokines by adipose tissue, adipose tissue matrix, and adipocytes from visceral and subcutaneous abdominal adipose tissues of obese humans ,
Endocrinology
 145 , 2273 , 2004
Cells
Adipocytes
Enzymes
Medium
see reference

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.