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Adrenal
Bovine
Bovine
Cena, V. , Garcia, A. , Montiel, C. and Sanchez-Garcia, P.
Uptake of [3H]-nicotine and [3H]-noradrenaline by Cultured Chromaffin Cells ,
Br J Pharmacol
 81 , 119 , 1984
Cells
Chromaffin
Enzymes
Medium
CF Kreb's
Adrenal
Bovine
Bovine
Waymire, J. , Bennett, W. , Boehme, R. , Hankins, L. , Gilmer-Waymire, K. and Haycock, J.
Bovine Adrenal Chromaffin Cells: High-Yield Purification and Viability in Suspension Culture ,
J Neurosci Methods
 7 , 329 , 1983
Cells
Chromaffin
Enzymes
Medium
HBSS, modified
Adrenal
Bovine
Bovine
Knight, D. and Baker, P.
Stimulus-Secretion Coupling in Isolated Bovine Adrenal Medullary Cells ,
Q J Exp Physiol
 68 , 123 , 1983
Cells
Medulla
Enzymes
Hyaluronidase : 0.2%
Medium
Saline w/BSA 0.5%
Adrenal
Bovine
Bovine
Greenberg, A. and Zinder, O.
alpha- and beta-Receptor Control of Catecholamine Secretion from Isolated Adrenal Medulla Cells ,
Cell Tissue Res
 226 , 655 , 1982
Cells
Medulla
Enzymes
Collagenase : 0.2%
Medium
Krebs-Ringer bicarbonate buffer, CMF
Adrenal
Bovine
Bovine
Wilson, S.P. and Viveros, O.H.
Primary Culture of Adrenal Medullary Chromaffin Cells in a Chemically Defined Medium ,
Exp Cell Res
 133 , 159 , 1981
Cells
Chromaffin
Enzymes
Deoxyribonuclease I : 15 µg/ml
Medium
Medium A
(see reference)
Adrenal
Bovine
Bovine
Baker, P. and Knight., D
Calcium Control of Exocytosis and Endocytosis in Bovine Adrenal Medullary Cells ,
Phil Trans R Soc Lond
 296 , 83 , 1981
Cells
Medulla
Enzymes
Protease : 0.2%
Medium
Saline
Adrenal
Bovine
Bovine
Trifaro, J.M. and Lee, R.W.
Morphological Characteristics and Stimulus-Secretion Coupling in Bovine Adrenal Chromaffin Cell Cultures ,
Neuroscience
 5 , 1533 , 1980
Cells
Chromaffin
Enzymes
Collagenase : 0.05%
Medium
Locke's solution, CMF
Adrenal
Bovine
Bovine, adult
(also rat, Hanover-Wistar, young; guinea-pig, newborn)
Unsicker, K. , Rieffert, B. and Ziegler, W.
Effects of Cell Culture Conditions, Nerve Growth Factor, Dexamethasone, and Cyclic AMP on Adrenal Chromaffin Cells In Vitro ,
Adv Biochem Psychopharmacol
 255 , 51 , 1980
Cells
Chromaffin
Enzymes
Collagenase : 0.5%
Collagenase : 0.5%
Medium
HBSS
Adrenal
Bovine
Bovine
Kumakura, K. , Karoum, F. , Guidotti, A. and Costa, E.
Modulation of Nicotinic Receptors by Opiate Receptor Agonists in Cultured Adrenal Chromaffin Cells ,
Nature
 283 , 489 , 1980
Cells
Chromaffin
Enzymes
Collagenase : 0.25%
Medium
F-12 medium
Adrenal
Bovine
Bovine
Kilpatrick, D. , Ledbetter, F. , Carson, K. , Kirshner, A. , Slepetis, R. and Kirshner, N.
Stability of Bovine Adrenal Medulla Cells in Culture ,
J Neurochem
 35 (3) , 679 , 1980
Cells
Medulla
Enzymes
Collagenase : 0.05%
Medium
Locke's solution, CF
Adrenal
Bovine
Bovine, adult
Unsicker, K. and Griesser, G.
Establishment, Characterization and Fibre Outgrowth of Isolated Bovine Adrenal Medullary Cells in Long-Term Cultures ,
Neuroscience
 5 , 1445 , 1980
Cells
Medulla
Enzymes
Medium
HBSS
Adrenal
Bovine
Bovine
Aunis, D. , Guerold, B. , Bader, M-F. and Cieselski-Treska, J.
Immunocytochemical and Biochemical Demonstration of Contractile Proteins in Chromaffin Cells ,
Neuroscience
 5 , 2261 , 1980
Cells
Chromaffin
Enzymes
Collagenase : 0.05%
Medium
DMEM
Adrenal
Bovine
Bovine
Hersey, R. and DiStefano, V.
Control of Phenylethanolamine N-Methyltransferase by Glucocorticoids in Cultured Bovine Adrenal Medullary Cells ,
J Pharmacol Exp Ther
 209 (1) , 147 , 1979
Cells
Medullary
Enzymes
Medium
HEPES
Adrenal
Bovine
Bovine
Folkman, J. , Haudenschild, C. C. and Zetter, B. R.
Long-term Culture of Capillary Endothlial Cells ,
Proc Natl Acad Sci U S A
 76 , 5217 , 1979
Cells
Foreskin
Enzymes
Collagenase : 0.5%
Medium
Dulbecco's MEM w/10% calf serum
Adrenal
Bovine
Bovine
Fenwick, E. , Fajdiga, P. , Howe, N. and Livett, B.
Functional and Morphological Characterization of Isolated Bovine Adrenal Medullary Cell ,
J Cell Biol
 76 , 12 , 1978
Cells
Medulla
Enzymes
Medium
Kreb's, CF
Adrenal
Bovine
Bovine, male
Schneider, A. , Herz, R. and Rosenheck, K.
Stimulus-Secretion Coupling in Chromaffin Isolated From Adrenal Medulla ,
Proc Natl Acad Sci U S A
 74 , 5036 , 1977
Cells
Chromaffin
Enzymes
Medium
Kreb's Ringer bicarbonate buffer, CF
Adrenal
Bovine
Bovine
Brooks, J.C.
The Isolated Bovine Adrenomedullary Chromaffin Cell: A Model of Neuronal Excitation-Secretion ,
Endocrinology
 101 , 1369 , 1977
Cells
Chromaffin
Enzymes
Hyaluronidase : 0.2%
Medium
HEPES, CF
Adrenal
Guinea-Pig
Guinea-pig, 500-700 g
Role, L.W. , Leeman, S.E. and Perlman, R.L.
Somatostain and Substance P Inhibit Catecholamine Secretion from Isolated Cells of Guinea-pig Adrenal Medulla ,
Neurochem Int
 6 , 1813 , 1981
Cells
Chromaffin
Medulla
Enzymes
Medium
BSS
(see reference)
Adrenal
Guinea-Pig
Guinea-pig
(also rat, Hanover-Wistar, young; newborn; cattle)
Unsicker, K. , Rieffert, B. and Ziegler, W.
Effects of Cell Culture Conditions, Nerve Growth Factor, Dexamethasone, and Cyclic AMP on Adrenal Chromaffin Cells In Vitro ,
Adv Biochem Psychopharmacol
 255 , 51 , 1980
Cells
Chromaffin
Enzymes
Collagenase : 0.5%
Collagenase : 0.5%
Medium
HBSS
Adrenal
Guinea-Pig
Guinea-pig
Hochman, J. and Perlman, R.L.
Catecholamine Secretion by Isolated Adrenal Cells ,
Biochim Biophys Acta
 421 , 168 , 1976
Cells
Adrenal
Chromaffin
Enzymes
Collagenase : 0.05%-0.20%
Medium
Kreb's-Ringer bicarb glucose buffer, CF
Adrenal
Hamster
Hamster (Mesocricetus auratus) 100-150 g
Liang, B.T. and Perlman, R.L.
Catecholamine Secretion by Hamster Adrenal Cells ,
J Neurochem
 32 , 927 , 1979
Cells
Adrenal
Chromaffin
Enzymes
Hyaluronidase : 0.20%
Medium
Kreb's Ringer bicarbonate buffer
Adrenal
Human
Human
Santana, M. , Chung, K. , Vukicevic, V. , Rosmaninho-Salgado, J. , Kanczkowski, W. , Cortez, V. , Hackmann, K. , Bastos, C. , Mota, A. , Schrock, E. , Bornstein, S. , Cavadas, C. and Ehrhart-Bornstein, M.
Isolation, Characterization, and Differentiation of Progenitor Cells from Human Adult Adrenal Medulla. ,
Stem Cells Transl Med
 1 , 783 , 2012
Cells
Adrenal medulla progenitor
Enzymes
Collagenase : 0.2%
Medium
DMEM/F-12
Adrenal
Human
Human
Jeon, Y. , Baek, W. , Chung, S. , Shin, N. , Kim, H. and Lee, S.
Cultured Human Chromaffin Cells Grafted in Spinal Subarachnoid Space Relieves Allodynia in a Pain Rat Model. ,
Korean J Anesthesiol
 60 , 357 , 2011
Cells
Chromaffin cells
Enzymes
Collagenase : 0.2%
Medium
Locke's solution
Adrenal
Human
Human
Caroccia, B. , Fassina, A. , Seccia, T. , Recarti, C. , Petrelli, L. , Belloni, A. , Pelizzo, M. and Rossi, G.
Isolation of Human Adrenocortical Aldosterone-Producing Cells by a Novel Immunomagnetic Beads Method. ,
Endocrinology
 151 , 1375 , 2010
Cells
Adrenocortical
Enzymes
Medium
Krebs Ringer
Adrenal
Human
Human, adult
Tischler, A. , DeLellis, R. , Bailes, B. , Nunnemacher, G.,Carabba, V. and Wolfe, H.
Nerve Growth Factor-Induced Neurite Outgrowth from Normal Human Chromaffin Cells ,
Lab Invest
 43 , 399 , 1980
Cells
Chromaffin
Enzymes
Trypsin : 0.25%
Medium
Eagle's MEM
Adrenal
Human
Human, adult and child
Folkman, J. , Haudenschild, C. C. and Zetter, B. R.
Long-term Culture of Capillary Endothlial Cells ,
Proc Natl Acad Sci U S A
 76 , 5217 , 1979
Cells
Foreskin
Enzymes
Collagenase : 0.5%
Medium
Dulbecco's MEM w/10% calf serum
Adrenal
Mouse
Mouse
Dominguez, N. , Rodri­guez, M. , Machado, J. and Borges, R.
Preparation and Culture of Adrenal Chromaffin Cells. ,
Methods Mol Biol
 846 , 223 , 2012
Cells
Chromaffin
Enzymes
Collagenase Type 1 : 0.1-0.2%
Deoxyribonuclease I : 0.003-0.015%
Hyaluronidase : 0.015%
Medium
DMEM/F12
Adrenal
Mouse
Mouse, 8-10 week
Kolski-Andreaco, A. , Cai, H. , Currle, D. , Chandy, K. and Chow, R.
Mouse Adrenal Chromaffin Cell Isolation. ,
J Vis Exp
 2 , 129 , 2007
Cells
Chromaffin
Enzymes
Papain : 40 u/ml
Medium
DMEM
File
11029.pdf (59.94 KB)
Adrenal
Mouse
Mouse, embryonic
Tian Jin-Hua, Wu Zheng-Xing, Unzicker Michael, Lu Li, Cai Qian, Li Cuiling, Schirra Claudia, Matti Ulf, Stevens David, Deng Chuxia, Rettig Jens, Sheng Zu-Hang.
The role of Snapin in neurosecretion: snapin knock-out mice exhibit impaired calcium-dependent exocytosis of large dense-core vesicles in chromaffin cells ,
J Neurosci
 25 , 10546-55 , 2005
Cells
Chromafin cells
Enzymes
Papain : 20-25 u/ml
Medium
DMEM
Adrenal
Ovine
Ovine, adult and fetal
Valego, N. and Rose, J.
A Specific CRH Antagonist Attenuates ACTH-Stimulated Cortisol Secretion in Ovine Adrenocortical Cells. ,
Reprod Sci
 17 , 477 , 2010
Cells
Adrenocortical
Enzymes
Medium
DMEM/Ham's F12
Adrenal
Ovine
Ovine, fetal
Valego, N. , Su, Y. , Carey, L. , Young, S. , Tatter, S. , Wang, J. and Rose, J.
Hypothalamic-Pituitary Disconnection in Fetal Sheep Blocks the Peripartum Increases in Adrenal Responsiveness and Adrenal ACTH Receptor Expression. ,
Am J Physiol Regul Integr Comp Physiol
 289 , R410 , 2005
Cells
Adrenocortical
Enzymes
Medium
DMEM/Ham's F12
Adrenal
Ovine
Ovine, adult
Keating, D. , Rychkov, G. , Adams, M. , Holgert, H. , McMillen, I.C. and Roberts, M.
Opioid Receptor Stimulation Suppresses the Adrenal Medulla Hypoxic Response in Sheep by Actions on Ca(2+) and K(+) Channels. ,
J Physiol
 555 , 489 , 2004
Cells
Chromaffin cells
Enzymes
Medium
Locke's solution
Adrenal
Ovine
Ovine, 3 year
Canny B J, O'Farrell K A, Clarke I J, Tilbrook A J.
The influence of sex and gonadectomy on the hypothalamo-pituitary-adrenal axis of the sheep ,
J Endocrinol
 162 , 215-25 , 1999
Cells
Anterior pituitary
Enzymes
Trypsin : 2.5%
Medium
DMEM
Adrenal
Rat
Rat
Dominguez, N. , Rodri­guez, M. , Machado, J. and Borges, R.
Preparation and Culture of Adrenal Chromaffin Cells. ,
Methods Mol Biol
 846 , 223 , 2012
Cells
Chromaffin
Enzymes
Collagenase Type 1 : 0.1-0.2%
Deoxyribonuclease I : 0.003-0.015%
Hyaluronidase : 0.015%
Medium
DMEM/F12
Adrenal
Rat
Rat
Gilabert, J, Montalvo, G. and Artalejo A.
Rat Chromaffin Cells Primary Cultures: Standardization and Quality Assessment for Single-Cell Assays ,
Nat Protoc
 , 294 , 2006
Cells
Chromaffin
Enzymes
Medium
HBSS
Adrenal
Rat
Rat, SD
Gilabert, J.
Necessary Conditions to Maintain Rat Adrenal Chromaffin Cells in Primary Culture , Cell Biology of the Chromaffin Cell , Borges, R. and Gandia, L. , Instituto Teofilo Hernando , 2004
Cells
Chromaffin cells
Enzymes
Medium
HBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.