Tissue References Search Tool

Rat

Ray, J. and Gage, F.

Neural Stem Cell Isolation, Characterization and Transplantation , Modern Techniques in Neuroscience Research , Johansson, H. and Windhorst, U. , Springer-Verlag New York , 339 , 1999

Cells

Central nervous system stem cells

Enzymes

Papain : 0.01%

Neutral Protease : 0.1%

Medium

DMEM/F-12

Rat

Rat, SD

Morrison, S. , White, P. , Zock, C. and Anderson, D.

Prospective Identification, Isolation by Flow Cytometry, and In Vivo Self-Renewal of Multipotent Mammalian Neural Crest Stem Cells ,
Cell
96 , 737 , 1999

Cells

Sciatic nerves

Enzymes

Trypsin : 0.025%

Collagenase Type 3 : 0.1%

Medium

L-15 medium

(see reference)

File

1099.pdf (350.54 KB)

Rat

Rat (also mice)

Johansson, C. , Momma, S. , Clarke, D. , Risling, M. , Lendahl, U. and Frisen, J

Identification of a Neural Stem Cell in the Adult Mammalian Central Nervous System ,
Cell
96 , 25 , 1999

Cells

Spinal cord

Enzymes

Trypsin : 0.133%

Medium

HBSS and PIPES

Rat

Rat, embryonic

Maric, D. , Maric, I. and Barker, J.

Buoyant Density Gradient Fractionation and Flow Cytometric Analysis of Embryonic Rat Cortical Neurons and Progenitor Cells. ,
Methods
16 , 247 , 1998

Cells

Neurons and progenitor

Enzymes

Papain : 20 u/ml

Medium

EBSS

Rat

Stemple, D. and Anderson, D.

Isolation of a Stem Cell for Neurons and Glia from the Mammalian Neural Crest ,
Cell
71 , 973 , 1992

Cells

Stem, neural crest

Enzymes

Collagenase : 0.075%

Medium

Ringer's solution

Human

Vandenabeele S, Hochrein H, Mavaddat N, Winkel K, Shortman K.

Human thymus contains 2 distinct dendritic cell populations ,
Blood
97 , 1733-41 , 2001

Cells

Dentritic

Enzymes

Collagenase Type 2 : 0.1%

Deoxyribonuclease I : 0.002%

Medium

RPMI 1640

Mouse

Rode, I. and Boehm, T.

Regenerative Capacity of Adult Cortical Thymic Epithelial Cells. ,
Proc Natl Acad Sci U S A
109 , 3463-8 , 2012

Cells

Thymic stromal

Enzymes

Collagenase Type 4 : 0.02%

Neutral Protease : 0.02%

Deoxyribonuclease I : 0.0025%

Medium

RPMI-1640

File

11532.pdf (1.16 MB)

Mouse

Phillips, Joy, Brondstetter, T. , English, C. , Lee, H. , Virts, E. and Thoman, M.

IL-7 Gene Therapy in Aging Restores Early thymopoiesis without Reversing Involution ,
J Immunol
, 4869 , 2004

Cells

Stromal

Enzymes

Collagenase Type 3 : 0.2%

Hyaluronidase : 0.1%

Medium

RPMI 1640

Mouse

Schiavoni, F, Mattei, F, Sestili, P, Borghi, P, Venditti, M, Morse, H, Belardelli, F. and Gabrieli, L

ICSBP is Essential for the Development of Mouse Type I Interferon-producing Cells and for the Generation and Activation of CD8a+ Dendritic Cells ,
J Exp Med
196 , 1415 , 2002

Cells

Dentritic

Enzymes

Collagenase Type 3 : 0.1%

Deoxyribonuclease I : 325 u/ml

Medium

RPMI 1640

Mouse

Mouse, 3-6 week

Smith KM, Olson DC, Hirose R, Hanahan D.

Pancreatic gene expression in rare cells of thymic medulla: evidence for functional contribution to T cell tolerance ,
Int Immunol
9 , 1355-65 , 1997

Cells

Thymic

Enzymes

Collagenase Type 3 : 100-400 u/ml

Medium

HBSS

Mouse

Mouse (BALB/c, C3H, C57BL/6), 1-28 day

Ropke, C. , van Deurs, B. and Petersen, O.

Short-term Cultivation of Murine Thymic Epithelial Cells in a Serum-Free Medium ,
In Vitro Cell Dev Biol
26 , 671 , 1990

Cells

Epithelial, thymus

Enzymes

Neutral Protease : 1.5 µg/ml

Medium

DMEM

Mouse

Mouse, C3H, 16 wk old, female

Ehmann, U. , Shiurba, R. and Peterson, W.

Long-Term Proliferation of Mouse Thymic Epithelial Cells in Culture ,
In Vitro Cell Dev Biol
22 (12) , 738 , 1986

Cells

Epithelial

Enzymes

Collagenase Type 3 : 0.1%

Medium

DMEM

Mouse

Mouse, Swiss, 6 wk

Jones, K. and Pierre, R.

Analysis of Cellular Heterogeneity in Mouse Thymus Cultures ,
In Vitro
17 , 431 , 1981

Cells

Thymus

Enzymes

Collagenase Type 3 : 150 u/ml

Medium

DMEM

Rat

Rat, postnatal

Bonfanti, P. , Claudinot, S. , Amici, A. , Farley, A. , Blackburn, C. and Barrandon, Y.

Microenvironmental Reprogramming of Thymic Epithelial Cells to Skin Multipotent Stem Cells. ,
Nature
466 , 978 , 2010

Cells

Thymic

Enzymes

Trypsin : 0.05%

Medium

HBSS

Rat

Masuda, A. and Matsuyama, M

Epithelial Cell Lines From Rat Thymoma and Rat Thymus , Cell & Tissue Culture: Laboratory Procedures Vol. 2 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 21C:4.1 , 1995

Cells

Thymic epithelial

Enzymes

Collagenase Type 3 : 0.1%

Medium

DMEM

Rat

Rat (ACI/NMs X BUF/Mna) F1, male, 28 months
Rat, (ACI/MNs) male, 8 weeks
(also bovine, adult)

Masuda, A. , Ohtsuka, K. and Matsuyama, M.

Establishment of Functional Epithelial Cell Lines from a Rat Thyoma and a Rat Thymus ,
In Vitro Cell Dev Biol
26 , 713 , 1990

Cells

Epthelial

Enzymes

Collagenase Type 3 : 0.1%

Medium

Eagle's MEM Serum-free

Bovine

Bovine, adult

Nygren, P. , Gylfe, E. , Larsson, R. , Johansson, H. , Juhlin, C. , Klareskoq, L. , Akerstrom, G. and Rastad, J.

Modulation of the Ca2+-Sensing Function of Parathyroid Cells In Vitro and in Hyperparathyroidism ,
Biochim Biophys Acta
968 , 253 , 1988

Cells

Parathyroid

Enzymes

Deoxyribonuclease I : 0.005%,

Medium

HEPES Ham's F10

Bovine

Wallace, J. and Scarpa, A.

Regulation of Parathyroid Hormone Secretion in Vitro by Divalent Cations ,
J Biol Chem
257 , 10613 , 1982

Cells

Parathyroid glands

Enzymes

Deoxyribonuclease I : 0.0075%

Medium

HEPES buffer

Bovine

Brown, E. , Hurwitz, S. and Aurbach, G.

Preparation of Viable Isolated Bovine Parathyroid Cells ,
Endocrinology
99 , 1582 , 1976

Cells

Parathyroid

Enzymes

Deoxyribonuclease I : 0.004%

Medium

Eagle's #2 medium without bicarbonate

Bovine

Bovine
(also porcine)

Tong, W.

The Isolation and Culture of Thyroid Cells ,
Meth Enzymol
32 , 745 , 1974

Cells

Thyroid

Enzymes

Trypsin : 0.004%

Medium

EBSS

Chicken

Chick embryo

Hilfer, S. and Brown, J.

Collagenase. Its Effectiveness as a Dispersing Agent for Embryonic Chick Thyroid and Heart ,
Exp Cell Res
65 , 246 , 1971

Cells

Thyroid

Muscle

Heart

Enzymes

Collagenase : 0.25%

Medium

Tyrode's saline, potassium free

Chicken

Chicken, Rhode Island Red, embryo

Spooner, B.

The Expression of Differentiation by Chick Embryo Throid in Cell Culture. I. Functional and Fine Structural Stability in Mass and Clonal Culture ,
J Cell Physiol
75 , 33 , 1970

Cells

Thyroid follicular

Enzymes

Collagenase : 0.2%

Medium

Tyrode's solution, CMF

Human

Yi, S. , Kim, H. , Koo, B. , Lee, S. , Choi, J. and Kang, Y.

Efficient Dissociation Protocol for Generation of Single Cell Suspension from Human Thyroid Tissue for Single Cell RNA Sequencing. , Endocrinol Metab (Seoul) Vol. 37 , 698-700 , 2022

Cells

Thyroid normal and tumor

Enzymes

Trypsin : 0.125%

Collagenase Type 4 : 300 u/ml

Medium

RPMI-1640

File

Yi.2022.pdf (1.89 MB)

Human

Patel, V. , Logan, A. , Watkinson, J. , Uz-Zaman, S. , Sheppard, M. , Ramsden, J. and Eggo, M.

Isolation and Characterization of Human Thyroid Endothelial Cells. ,
Am J Physiol Endocrinol Metab
284 , E168 , 2003

Cells

Endothelial

Enzymes

Collagenase Type 2 : 0.1%

Medium

DMEM

File

10586.pdf (413.23 KB)

Human

Gianoukakis, A. , Cao, H. , Jennings, T. and Smith, T.

Prostaglandin Endoperoxide H Synthase Expression in Human Thyroid Epithelial Cells. ,
Am J Physiol Cell Physiol
280 , C701 , 2001

Cells

Thyrocytes

Enzymes

Collagenase Type 1 : 130 u/ml

Neutral Protease : 0.5 u/ml

Medium

HBSS

File

10594.pdf (342.02 KB)

Human

Howie, A. , Walker, S. , Akesson, B. , Arthur, J. and Beckett, G.

Thyroidal Extracellular Glutathione Peroxidase: A Potential Regulator of Thyroid-Hormone Synthesis. ,
Biochem J
308 ( Pt 3) , 713 , 1995

Cells

Thyrocytes

Enzymes

Neutral Protease : 0.5%

Trypsin : 0.25%

Collagenase : 0.1%

Medium

EBSS

File

10593.pdf (1.03 MB)

Human

Miller, R. , Hiraoka, T. , Nakamura, N. , Tenou, H. , Kopecky, K. , Jones, M. and Gould, M.

In Vitro Culture of Human Thyroid Cells; Methods and Application to Radiation Biology. ,
J Radiat Res
26 , 269 , 1985

Cells

Thyroid

Enzymes

Collagenase : 300 u/ml

Medium

Ham's F-12/MEM

Mouse

Kitajima, S. , Kohno, S. , Kondoh, A. , Sasaki, N. , Nishimoto, Y. , Li, F. , Mohammed, M. , Muranaka, H. , Nagatani, N. , Suzuki, M. , Kido, Y. and Takahashi, C.

Undifferentiated State Induced by Rb-p53 Double Inactivation in Mouse Thyroid Neuroendocrine Cells and Embryonic Fibroblasts. ,
Stem Cells
33 , 1657-69 , 2015

Cells

Thyroid tumor

Enzymes

Collagenase Type 1 : 300 u/ml

Hyaluronidase : 100 u/ml

Deoxyribonuclease I : 0.01%

Medium

DMEM

File

11653.pdf (5.57 MB)

Mouse

Mouse, 6 week

Martin, A. , Coronel, E. , Sano, G. , Chen, S. , Vassileva, G. , Canasto-Chibuque, C. , Sedgwick, J. , Frenette, P. , Lipp, M. , Furtado, G. and Lira, S.

A Novel Model for Lymphocytic Infiltration of the Thyroid Gland Generated by Transgenic Expression of the CC Chemokine CCL21. ,
J Immunol
173 , 4791 , 2004

Cells

Thyroid

Enzymes

Neutral Protease : 0.0012 u/ml

Collagenase Type 2 : 0.25 u/ml

Medium

RPMI 1640