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Brain
Insect
Drosophila
Gu, H. and O'Dowd, D.
Whole Cell Recordings from Brain of Adult Drosophila. ,
J Vis Exp
 6 , 248 , 2007
Cells
Neurons
Enzymes
Papain : 20 u/ml
Medium
Saline
File
10651.pdf (266.1 KB)
Brain
Insect
Drosophila
Sicaeros, B. Campusano, J. and O'Dowd, D.
Primary Neuronal Cultures from the Brains of Late Stage Drosophila Pupae. ,
J Vis Exp
 4 , 200 , 2007
Cells
Neurons
Enzymes
Papain : 4 u/ml
Medium
DMEM
File
10989.pdf (77.81 KB)
Brain
Insect
Drosophilia
Gu, H. and O'Dowd, D.
Cholinergic Synaptic Transmission in Adult Drosophila Kenyon Cells in Situ. ,
J Neurosci
 26 , 265-72 , 2006
Cells
Kenyon
Enzymes
Papain : 20 u/ml
Medium
Recording saline
File
11594.pdf (411.4 KB)
Brain
Monkey
Monkey, rhesus
Marcondes MC, Burudi EM, Huitron-Resendiz S, Sanchez-Alavez M, Watry D, Zandonatti M, Henriksen SJ, Fox HS.
Highly activated CD8(+) T cells in the brain correlate with early central nervous system dysfunction in simian immunodeficiency virus infection ,
J Immunol
 167 , 5429-38 , 2001
Cells
Brain cells
Enzymes
Medium
HBSS
Brain
Mouse

Mouse, adult

Joseph, D. , Deimling, M. , Hasegawa, Y. , Cristancho, A. , Risbud, R. , McCoy, A. and Marsh, E.
Protocol for Isolating Young Adult Parvalbumin Interneurons from the Mouse Brain for Extraction of High-Quality RNA. , STAR Protoc Vol. 2 , 100714 , 2021
Cells
Interneurons
Enzymes
Medium
EBSS
Brain
Mouse
Adult and neonatal
Calvo, Belen, Rubio, F. , Fernandez, M. and Tranque, P.
Dissociation of Neonatal and Adult Mice Brain for Simultaneous Analysis of Microglia, Astrocytes and Infiltrating Lymphocytes by Flow Cytometry. , IBRO Rep Vol. 8 , 36-47 , 2020
Cells
Microglia, astrocytes, lymphocytes
Enzymes
Collagenase Type 2 : 120 u/ml
Papain : 20 u/ml
Neutral Protease : 1.2 u/ml
Medium
EBSS
File
11791.pdf (4.11 MB)
Brain
Mouse
Mouse, adult
Swartzlander, D. , Propson, N. , Roy, E. , Saito, T. , Saido, T. , Wang, B. and Zheng, H.
Concurrent Cell Type-Specific Isolation and Profiling of Mouse Brains in Inflammation and Alzheimer's Disease. ,
JCI Insight
 3 , 2018
Cells
Astrocytes, microglia, endothelia, oligodendrocytes
Enzymes
Papain : 20 u/ml
Medium
HBSS
File
11716.pdf (2.31 MB)
Brain
Mouse
Mouse
Kramer, D. , Risso, D. , Kosillo,. , Ngai, J. and Bateup, H.
Combinatorial Expression of Grp and Neurod6 Defines Dopamine Neuron Populations with Distinct Projection Patterns and Disease Vulnerability , eNeuro Vol. 5 , 1 , 2018
Cells
Neurons
Enzymes
Papain : 10 u/ml
Medium
Artificial CSF
File
11812.pdf (3.24 MB)
Brain
Mouse
Mouse, fetal and mature
Zhang, Y. , Sloan, S. , Clarke, L. , Caneda, C. , Plaza, C. , Blumenthal, P. , Vogel, H. , Steinberg, G. , Edwards, M. , Li, G. , Duncan, J. , Cheshier, S. , Shuer, L. , Chang, E. , Grant, G. , Gephart, M. and Barres, B.
Purification and Characterization of Progenitor and Mature Human Astrocytes Reveals Transcriptional and Functional Differences with Mouse. ,
Neuron
 89 , 37-53 , 2016
Cells
Astrocytes and neurons
Enzymes
Papain : 7.5-20 u/ml
Medium
RPMI
File
11490.pdf (4.03 MB)
Brain
Mouse
Mouse
Baruch, K. , Rosenzweig, N. , Kertser, A. , Deczkowska, A. , Sharif, A. , Spinrad, A. , Tsitsou-Kampeli, A. , Sarel, A. , Cahalon, L. and Schwartz, M.
Breaking Immune Tolerance by Targeting Foxp3(+) Regulatory T Cells Mitigates Alzheimer's Disease Pathology. ,
Nat Commun
 6 , 7967 , 2015
Cells
Chloroid plexus
Enzymes
Collagenase Type 4 : 400 u/ml
Medium
PBS
File
11428.pdf (2.03 MB)
Brain
Mouse
Mouse, 3 week
Luo,Y.,Coskun, V. , Liang, A.,Yu, J. , Cheng, L.,Ge, W. , Shi, Z. , Zhang, K. , Li, C. , Lin, H. , Luo, D. , Wang, J. , Lin, C. , Dai, Z. , Zhu, H. , Zhang, J. , Liu, J. , Liu, H.,deVellis, J. , Horvath, S. , Sun, Y. and Li, S.
Different Patterns of Molecular Forms of Somatostatin are Released by the Rat Median Eminence and Hypothalamus. ,
Cell
 161 , 1175 , 2015
Cells
Neural
Enzymes
Papain : 20 u/ml
Medium
PBS
File
11529.pdf (4.05 MB)
Brain
Mouse
Mouse
Ryg-Cornejo, V. , Ioannidis, L. and Hansen, D.
Isolation and Analysis of Brain-Sequestered Leukocytes from Plasmodium Berghei ANKA-Infected Mice. ,
J Vis Exp
 71 , e50112 , 2013
Cells
Brain-sequestered leukocytes
Enzymes
Medium
RPMI
File
10908.pdf (602.58 KB)
Brain
Mouse
Mouse
Shah. G. , Price, T. , Banks, W. , Morofuji, Y. , Kovac, A. , Ercal, N. , Sorenson, C. , Shin, E. and Sheibani, N.
Pharmacological Inhibition of Mitochondrial Carbonic Anhydrases Protects Mouse Cerebral Pericytes from High Glucose-Induced Oxidative Stress and Apoptosis. ,
J Pharmacol Exp Ther
 344 , 637-45 , 2013
Cells
Cerebral pericytes
Enzymes
Medium
DMEM
File
11080.pdf (786.33 KB)
Brain
Mouse
Mouse, P13-15
Haseleu, J. , Anlauf, E. , Blaess, S. , Endl, E. and Derouiche, A.
Studying Subcellular Detail in Fixed Astrocytes: Dissociation of Morphologically Intact Glial Cells (DIMIGs). ,
Front Cell Neurosci
 7 , 54 , 2013
Cells
Glial
Enzymes
Papain : 20 u/ml
Medium
EBSS
File
11388.pdf (2.72 MB)
Brain
Mouse
Mouse, P0-P15
Foo, L.
Purification of Rat and Mouse Astrocytes by Immunopanning. ,
Cold Spring Harb. Protoc.
 2013 , 421-32 , 2013
Cells
Astrocytes
Enzymes
Papain : 5 u/ml
Medium
DPBS
File
11541.pdf (600.89 KB)
Brain
Mouse
Mouse, adult
Saxena, A. , Wagatsuma, A. , Noro, Y. , Kuji, T. , Asaka-Oba, A. , Watahiki, A. , Gurnot, C. , Fagiolini, M. , Hensch, T. and Carninci, P.
Trehalose-Enhanced Isolation of Neuronal Sub-Types from Adult Mouse Brain. ,
Biotechniques
 52 , 381 , 2012
Cells
Neurons
Enzymes
PDS kit : with modifications
Medium
DMEM/F12
File
10801.pdf (2.89 MB)
Brain
Mouse
Mouse, 1-2 day
O'Meara, R. , Ryan, S. , Colognato, H. and Kothary, R.
Derivation of Enriched Oligodendrocyte Cultures and Oligodendrocyte/Neuron Myelinating Co-Cultures from Post-Natal Murine Tissues. ,
J Vis Exp
 54 , 3324 , 2011
Cells
Oligodendrocytes, dorsal root ganglia
Enzymes
Papain : 0.15%
Medium
DMEM
File
10650.pdf (1.02 MB)
Brain
Mouse
Mouse, 6-12 week
Chung, W. , Farley, J. , Swenson, A. , Barnard, J. , Hamilton, G. , Chiposi, R. and Drummond, H.
Extracellular Acidosis Activates ASIC-like Channels in Freshly Isolated Cerebral Artery Smooth Muscle Cells. ,
Am J Physiol Cell Physiol
 298 , C1198 , 2010
Cells
Vascular smooth muscle cells
Enzymes
Papain : 0.05%
Elastase : 0.05%
Medium
PBS
Brain
Mouse
Mouse, 4-6 day
Lee, H. , Greene, L. , Mason, C. and Manzini, M.
Isolation and Culture of Post-Natal Mouse Cerebellar Granule Neuron Progenitor Cells and Neurons. ,
J Vis Exp
 23 , 990 , 2009
Cells
Neurons
Enzymes
PDS kit : with modifications
Medium
HBSS
File
10652.pdf (245 KB)
Brain
Mouse
Mouse, postnatal
Sher, F. , Rossler, R. , Brouwer, N. , Balasubramaniyan, V. , Boddeke, E. and Copray, S.
Differentiation of Neural Stem Cells Into Oligodendrocytes: Involvement of the Polycomb Group Protein Ezh2. ,
Stem Cells
 26 , 2875 , 2008
Cells
Astrocytes
Enzymes
Trypsin : 0.25%
Deoxyribonuclease I : 1,000 u/ml
Medium
HBSS
Brain
Mouse
Mouse, neonatal
Fasano, C. , Thibault, D. and Trudeau, L.
Culture of Postnatal Mesencephalic Dopamine Neurons on an Astrocyte Monolayer , Current Protocols in Neuroscience Vol. 44 , 3.21.1 , 2008
Cells
Neurons
Enzymes
Papain : 20 u/ml
Medium
Neurobasal
Brain
Mouse
Mouse, embryonic and postnatal
Hilgenberg, L. and Smith, M.
Preparation of Dissociated Mouse Cortical Neuron Cultures. ,
J Vis Exp
 10 , 562 , 2007
Cells
Cortical neurons
Enzymes
Papain : 4-10 u/ml
Medium
Neurobasal
File
10660.pdf (290.43 KB)
Brain
Mouse
Mouse, 1 day
Seaberg, R. , Smukler, S. and Van der Kooy, D.
Intrinsic Differences Distinguish Transiently Neurogenic Progenitors from Neural Stem Cells in the Early Postnatal Brain. ,
Dev Biol
 278 , 71 , 2005
Cells
Neural progenitor
Enzymes
PDS kit : per instructions
Medium
see reference
Brain
Mouse
Mouse
Oliver, T. , Read, T. , Kessler, J. , Mehmeti, A. , Wells, J. , Huynh, T. , Lin, S. and Wechsler-Reya, R.
Loss of Patched and Disruption of Granule Cell Development in a Pre-Neoplastic Stage of Medulloblastoma. ,
Development
 132 , 2425 , 2005
Cells
Granule cell precursors, pre-neoplastic and tumor cells
Enzymes
Papain : 10 u/ml
Deoxyribonuclease I : 250 u/ml
Medium
Neurobasal/B27
Brain
Mouse
Mouse
Klein C, Butt SJ, Machold RP, Johnson JE. and Fishell G.
Cerebellum- and forebrain-derived stem cells possess intrinsic regional character ,
Development
 132 , 4497 , 2005
Cells
Neurosperes
Enzymes
PDS kit : per instructions
Medium
DMEM/F12
Brain
Mouse
Mouse
Hernandez, F. , Perez, M. , Lucas, J. , Mata, A. , Bhat, R. and Avila, J.
Glycogen Synthase Kinase-3 plays a Crucial Role in Tau Exon 10 Splicing and Intranuclear Distribution of SC35. Implications for Alzheimer's Disease. ,
J Biol Chem
 279 , 3801 , 2004
Cells
Cortical neurons
Enzymes
PDS kit : per instructions
Medium
Neurobasal
Brain
Mouse
Mouse, 2-5 day
Smeyne Michelle, Smeyne RichardJ.
Method for culturing postnatal substantia nigra as an in vitro model of experimental Parkinson's disease ,
Brain Res Brain Res Protoc
 9 , 105-11 , 2002
Cells
Postnal substantia nigra
Enzymes
PDS kit : per instructions
Medium
see reference
Brain
Mouse
Mouse, embryonic
Estivill-Torrus, G. , Pearson, H. , Van Heyningen, V. , Price, D. and Rashbass, P.
Pax6 is Required to Regulate the Cell Cycle and the Rate of Progression from Symmetrical to Asymmetrical Division in Mammalian Cortical Progenitors. ,
Development
 129 , 455 , 2002
Cells
Cortical progenitors
Enzymes
PDS kit : per instructions
Medium
Serum free medium
Brain
Mouse
Mouse, 3 day
Nishioku T, Hashimoto K, Yamashita K, Liou SY, Kagamiishi Y, Maegawa H, Katsube N, Peters C, von Figura K, Saftig P, Katunuma N, Yamamoto K, Nakanishi H.
Involvement of cathepsin E in exogenous antigen processing in primary cultured murine microglia ,
J Biol Chem
 277 , 4816 , 2002
Cells
Microglia
Enzymes
Papain : 90 u/ml
Deoxyribonuclease I : 2000 u/ml
Medium
Eagle's MEM
Brain
Mouse
Mouse, SD, 8-12 week
O'Donnell SL, Frederick TJ, Krady JK, Vannucci SJ, Wood TL.
IGF-I and microglia/macrophage proliferation in the ischemic mouse brain ,
Glia
 39 , 85 , 2002
Cells
Microglia
Enzymes
Medium
RPMI-1640
Brain
Mouse
Mouse, 1 day
Martin-Aparicio, E. , Yamamoto, A. , Hernandez, F. , Hen, R. , Avila, J. and Lucas, J.
Proteasomal-Dependent Aggregate Reversal and Absence of Cell Death in a Conditional Mouse Model of Huntington's Disease. ,
J Neurosci
 21 , 8772 , 2001
Cells
Neurons and glia
Enzymes
PDS kit : per instructions
Medium
Neurobasal
Brain
Mouse
Mouse
Miyazawa K, Himi T, Garcia V, Yamagishi H, Sato S. and Ishizaki Y.
A role for p27/Kip1 in the control of cerebellar granule cell precursor proliferation ,
J Neurosci
 20 , 5756 , 2000
Cells
Cerebellar granule cell precursors
Enzymes
Papain : 0.435%
Medium
EBSS
Brain
Mouse
Mouse
Lim, D. and Alvarez-Buylla, A.
Interaction Between Astrocytes and Adult Subventricular Zone Precursors Stimulates Neurogenesis ,
Proc Natl Acad Sci U S A
 96 , 7526 , 1999
Cells
Astrocytes
Enzymes
Trypsin : 0.25%
Medium
DMEM
Brain
Mouse
Mouse, newborn
Jun K, Choi G, Yang SG, Choi KY, Kim H, Chan GC, Storm DR, Albert C, Mayr GW, Lee CJ, Shin HS.
Enhanced hippocampal CA1 LTP but normal spatial learning in inositol 1,4,5-trisphosphate 3-kinase(A)-deficient mice ,
Learn Mem
 5 , 317-30 , 1998
Cells
Hippocampal cells
Enzymes
Papain : 10 u/ml
Medium
DMKM
Brain
Mouse
Mouse, SWR or CF1, 1-3 months
Spielman, A. , Mody, I. , Brand, J. , Whitney, G. , MacDonald, J. and Salter, M.
A Method for Isolating and Patch-Clamping Single Mammalian Taste Receptor Cells ,
Brain Res
 503 , 326 , 1989
Cells
Papillae, taste receptor
Enzymes
Pronase E : 0.15%
Medium
Carbonate-Phosphate buffer
(see reference)
Brain
Mouse
Mouse, 0-30 day
Shrier, B. , Wilson, S. and Nirenberg, M.
Cultured Cell Systems and Methods for Neurobiology , Vol. 32 , 765 , 1974
Cells
Neural
Enzymes
Medium
BSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.