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Tissue References Search Tool

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Neural
Rat
Rat, Fisher, 8-9 week
Lie DC, Dziewczapolski G, Willhoite AR, Kaspar BK, Shults CW, Gage FH.
The adult substantia nigra contains progenitor cells with neurogenic potential ,
J Neurosci
 22 , 6639 , 2002
Cells
Adult progenitor
Enzymes
Papain : 2.5 u/ml
Deoxyribonuclease I : 250 u/ml
Neutral Protease : 1 u/ml
Medium
DMEM/F-12
Neural
Rat
Rat
Liu, Q. , Kawai, H. and Berg, D.
B-Amyloid Peptide Blocks the Response of a7-Containing Nicotinic Receptors on Hippocampal Neurons ,
PNAS
 98 (8) , 4734 , 2001
Cells
Neurons, hippocampal
Enzymes
Papain : 15 - 20 u/ml
Medium
Eagle's MEM
(see reference)
Neural
Rat
Rat, embryonic, day 18
O'Connor, S. , Andreadis, J. , Shaffer, K. , Ma, W. , Pancrazio, J. and Stenger, D.
Immobilization of Neural Cells in Three-Dimensional Matrices for Biosensor Applications ,
Biosensors & Bioelectronics
 14 , 871 , 2000
Cells
Cortical
Medium
Neurobasal medium and DMEM
Neural
Rat
Rat, Wister, postnatal, P1-3 days
Neuhoff, H. , Roeper, J. and Schweizer, M.
Activity-Dependent Formation of Perforated Synapses in Cultured Hippocampal Neurons ,
Eur J Neurosci
 11 , 4241 , 1999
Cells
Neurons, hippocampal
Enzymes
Papain : 20 u/ml
Medium
EBSS
Neural
Rat
Rat, (Long Evans), 2-5 day old
Wilding, T. and Huettner, J.
Activation and Desensitization of Hippocampal Kainate Receptors ,
J Neurosci
 17 (8) , 2713 , 1997
Cells
Neurons, hippocampal
Enzymes
Papain : 20 u/ml
Medium
EBSS
Neural
Rat
Rat, pups, 24-48 h old
Hall, R. and Soderling, T.
Differential Surface Expression and Phosphorylation of the N-Methyl-D-Aspartate Receptor NR1 and NR2 in Cultured Hippocampal Neurons ,
J Biol Chem
 272 (7) , 4135 , 1997
Cells
Neurons, hippocampal
Enzymes
Papain : 20 u/ml
Medium
Harvest buffer
Neural
Rat
Rat, SD, Fisher
Brewer, G.J.
Isolation and culture of adult rat hippocampal neurons of any age ,
J Neurosci Methods
 71 , 143 , 1997
Cells
Hippocampal neurons
Enzymes
Papain : 0.2%
Medium
HibernateA/B27
Neural
Rat
Rat, 1-4 day old
Twitchell, W. , Brown, S. and Mackie, K.
Cannabinoids Inhibit N- and P/Q-Type Calcium Channels in Cultured Rat Hippocampal Neurons ,
J Neurophysiol
 78 , 43 , 1997
Cells
Neurons, hippocampal
Enzymes
Papain : 20 u/ml
Medium
MEM
Neural
Rat
Rat, E18
Liu QY, Schaffner AE, Li YX, Dunlap V, Barker JL.
Upregulation of GABAA current by astrocytes in cultured embryonic rat hippocampal neurons ,
J Neurosci
 16 , 2912-23 , 1996
Cells
Hippocampal neurons
Enzymes
Papain : 20 u/ml
Medium
MEM
Neural
Rat
Rat, 1-6 day
Robertson, S. , Rae, M. , Rowan, E. and Kennedy, C.
Characterization of a P2X-Purinoceptor in Cultured Neurones of the Rat Dorsal Root Ganglia. ,
Br J Pharmacol
 118 , 951 , 1996
Cells
DRG neurons
Enzymes
Papain : 20 u/ml
Collagenase Type 1 : 150 u/ml
Medium
HBSS
Neural
Rat
Rat, postnatal
Hatanaka,H. , Tsukui, H. and Nihonmatsu, I.
Septal Cholinergic Neurons From Postnatal Rat Can Survive In The Dissociate Culture Conditions In The Presence Of Nerve Growth Factor ,
Neurosci Lett
 79 , 85 , 1987
Cells
Septal neurons
Enzymes
Papain : 0.05%
Medium
PBS, CMF
Neural
Rat
Rat, Long Evans, 1-15 days
Huettner, J. and Baughman, R.
Primary Culture of Identified Neurons From the Visual Cortex of Postnatal Rats ,
J Neurosci
 6 , 3044 , 1986
Cells
Neurons, visual cortex
Enzymes
Papain : 20 u/ml
Medium
BSS
(see reference)
Neural
Rat
Rat, postnatal
Leifer, D. , Lipton, S. , Barnstable, C. and Masland, R.
Monoclonal Antibody to Thy-1 Enhances Regeneration of Processes by Rat Retinal Ganglion Cells in Culture ,
Science
 224 , 303 , 1984
Cells
Ganglion, retina
Enzymes
Papain : 12.5 u/ml
Medium
HBSS w/5 mM HEPES
Neural
Salamander
Salamander, 18-25 cm
Townes-Anderson, E. , MacLeish, P. and Raviola, E.
Rod Cells Dissociated from Mature Salamander Retina: Ultrastruture and Uptake of Horseradish Peroxidase ,
J Cell Biol
 100 , 175 , 1985
Cells
Retina
Enzymes
Papain : 14 u/ml
Medium
Saline
Neural
Salamander
Salamander (A.tigrinum)
Bader, C. , MacLeish, P. and Schwartz, E.
Responses to Light of Solitary Rod Photoreceptors Isolated From Tiger Salamander Retina ,
Proc Natl Acad Sci U S A
 75 , 3507 , 1978
Cells
Photoreceptors, retina
Enzymes
Papain : 0.05%
Medium
(see reference)
Neural
Turtle
Turtle (Pseudemys scripta elegans)
Lam, D.
Biosynthesis of Acetylcholine in Turtle Photoreceptors ,
Proc Natl Acad Sci U S A
 69 , 1987 , 1972
Cells
Retinal
Enzymes
Papain : 0.1% (13.5 u/mg)
Medium
Kreb's Ringer
Organoids
Human

Human

Geest, A. , Jakobs, C. , Ljubikj, T. , Huffels, C. , Luna, M. , Sá, R. , Adolfs, Y. , Wit, M. , Rutten, D. , Kaal, M. , Zwartkruis, M. , Carcolé, M. , Groen, E. , Hol, E. , Basak, O. , Isaacs, A. , Westeneng, H. , Berg, L. , Veldink, J. , Schlegel, D. and Pasterkamp, R.
Molecular Pathology, Developmental Changes, and Synaptic Dysfunction in (pre-) Symptomatic Human C9ORF72-ALS/FTD Cerebral Organoids. , Acta Neuropathol Commun Vol. 12 , 152 , 2024
Cells
Cerebral organoids
Enzymes
Papain : 20 u/ml
Medium
DMEM/F12
Organoids
Human

Human

Lisowski, P. , Lickfett, S. , Rybak-Wolf, A. , Menacho, C. and Le, S.
Mutant Huntingtin Impairs Neurodevelopment in Human Brain Organoids through CHCHD2-mediated Neurometabolic Failure. ,
Nat Commun
 15 , 7027 , 2024
Cells
Brain organoids
Enzymes
Papain : 20 u/ml
Medium
EBSS
Pancreas
Human
Human
Cane, M. , Sutton, R. and Criddle, D.
Isolation of Human Pancreatic Acinar Cells From Rat and Human Pancreas ,
Pancreapedia
 20 , 1 , 2011
Cells
Acinar
Enzymes
CLSPA : 200 u/ml
Medium
See Reference
Pancreas
Mouse
Mouse, adult, male
Ji, B. , Gaiser, S. , Chen, X. , Ernst, S. and Logsdon, C.
Intracellular Trypsin Induces Pancreatic Acinar Cell Death but not NF-KappaB Activation. ,
J Biol Chem
 284 , 17488 , 2009
Cells
Ancinar
Enzymes
CLSPA : see reference
Medium
DMEM
Pancreas
Mouse
Mouse, 6-10 month
Wang, Y. , Soyombo, A. , Shcheynikov, N. , Zeng, W. , Dorwart, M. , Marino, C. , Thomas, P. and Muallem, S.
Slc26a6 Regulates CFTR Activity In Vivo to Determine Pancreatic Duct HCO3- Secretion: Relevance to Cystic Fibrosis. ,
EMBO J
 25 , 5049 , 2006
Cells
Pancreatic ductal
Enzymes
CLSPA : 50 u/ml
Hyaluronidase : 400 u/ml
Medium
DMEM
Pancreas
Mouse
Mouse, adult
Voronina, S. , Barrow, S. , Gerasimenko, O. , Petersen, O. and Tepikin, A.
Effects of Secretagogues and Bile Acids on Mitochondrial Membrane Potential of Pancreatic Acinar Cells: Comparison of Different Modes of Evaluating Delta Psi. ,
J Biol Chem
 279 , 27327 , 2004
Cells
Acinar
Enzymes
CLSPA : 200 u/ml
Medium
see reference
Pancreas
Mouse
Mouse, albino, 25 g
Fogarty, K. , Kidd, J. , Tuft, R. and Thorn, P.
A Bimodal Pattern of InsP(3)-Evoked Elementary Ca(2+) Signals in Pancreatic Acinar Cells. ,
Biophys J
 78 , 2298 , 2000
Cells
Acinar
Enzymes
CLSPA : see reference
Medium
see reference
Pancreas
Mouse
Mouse
Toivola, D. , Ku, N. , Ghori, N. , Lowe, A. , Michie, S. and Omary, M.
Effects of Keratin Filament Disruption on Exocrine Pancreas-Stimulated Secretion and Susceptibility to Injury. ,
Exp Cell Res
 255 , 156 , 2000
Cells
Acinar cells and acini
Enzymes
Collagenase Type 1 : see reference
CLSPA : see reference
Medium
see reference
Pancreas
Mouse
Mouse
Githens, S.
Pancreatic Duct Epithelial Cells , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12B:12.1 , 1995
Cells
Duct
Enzymes
Papain : 25 u/ml
Medium
DMEM /F-12
Pancreas
Mouse
Mouse, male, 18-24 g
Burnham DB, Williams JA.
Effects of carbachol, cholecystokinin, and insulin on protein phosphorylation in isolated pancreatic acini ,
J Biol Chem
 257 , 10523-8 , 1982
Cells
Acinar
Enzymes
CLSPA : 70-90 u/ml
Medium
Krebs-Henseleit
Pancreas
Rat
Rat
Williams, J.
Isolation of Rodent Pancreatic Acinar Cells and Acini by Collagenase Digestion ,
Pancreapedia
 , 2010
Cells
Acinar
Enzymes
CLSPA : 100 u/ml
Medium
DMEM
Pancreas
Rat
Rat, SD, male
Ji B, Kopin AS, Logsdon CD.
Species differences between rat and mouse CCKA receptors determine the divergent acinar cell response to the cholecystokinin analog JMV-180 ,
J Biol Chem
 275 , 19115-20 , 2000
Cells
Ancinar
Enzymes
CLSPA : see reference
Medium
DMEM
Pancreas
Rat
Rat
Blinman TA, Gukovsky I, Mouria M, Zaninovic V, Livingston E, Pandol SJ, Gukovskaya AS.
Activation of pancreatic acinar cells on isolation from tissue: cytokine upregulation via p38 MAP kinase ,
Am J Physiol Cell Physiol
 279 , C1993-2003 , 2000
Cells
Pancreatic acini
Enzymes
CLSPA : 30 u/ml
Collagenase Type 4 : 30 u/ml
Medium
M199
Pancreas
Rat
Rat
Githens, S.
Pancreatic Duct Epithelial Cells , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12B:12.1 , 1995
Cells
Duct
Enzymes
Papain : 25 u/ml
Medium
DMEM /F-12
Pancreas
Rat
Rat, SD, both sexes, 6-20 wks old
Githens, S. , Schexnayder, J. , Desai, K. and Patke, C.
Rat Pancreatic Interlobular Duct Epithelium: Isolation and Culture in Collagen Gel ,
In Vitro Cell Dev Biol
 25 (8) , 679 , 1989
Cells
Interlobular ducts
Enzymes
Papain : 25 u/ml
Medium
DMEM/Ham's F-12
Stem
Human
Human
Rigamonti, A. , Repetti, G. , Sun, C. , Price, F. , Reny, D. , Rapino, F. , Weisinger, K. , Benkler, C. , Peterson, Q. , Davidow, L. , Hansson, E. and Rubin, L.
Large-Scale Production of Mature Neurons from Human Pluripotent Stem Cells in a Three-Dimensional Suspension Culture System. ,
Stem Cell Reports
 6 , 993-1008 , 2016
Cells
Motor neurons
Enzymes
Papain : 20 u/ml
Medium
EBSS
Stem
Human
Human
Welter, J. and Solchaga, L.and Penick, K.
Simplification of Aggregate Culture of Human Mesenchymal Stem Cells as a Chondrogenic Screening Assay ,
Biotechniques
 42 , 732 , 2007
Cells
Bone marrow derived MSC
Enzymes
Trypsin : 0.05%
Papain : 0.0025%
Medium
DMEM
Stem
Insect
Drosophila, larval
Berger, C. , Harzer, H. , Burkard, T. , Steinmann, J. , van der Horst, S. , Laurenson, A. , Novatchkova, M. , Reichert, H. and Knoblich, J.
FACS Purification and Transcriptome Analysis of Drosophila Neural Stem Cells Reveals a Role for Klumpfuss in Self-Renewal. ,
Cell Rep
 2 , 407 , 2012
Cells
Neuroblasts
Enzymes
Papain : 0.1%
Medium
Rinaldini solution
Stem
Mouse
Mouse, 6-8 week
Bracko, O. , Singer, T. , Aigner, S. , Knobloch, M. , Winner, B. , Ray, J. , Clemenson, G. , Suh, H. , Couillard-Despres, S. , Aigner, L. , Gage, F. and Jessberger, S.
Gene Expression Profiling of Neural Stem Cells and their Neuronal Progeny Reveals IGF2 as a Regulator of Adult Hippocampal Neurogenesis. ,
J Neurosci
 32 , 3376-87 , 2012
Cells
Neural stem cells
Enzymes
Medium
DMEM/F12
Stem
Mouse
Mouse, P4-5
Ziegler, A. , Schneider, J. , Qin, M. , Tyler, W. , Pintar, J. , Fraidenraich, D. , Wood, T. and Levison, S.
IGF-II Promotes Stemness of Neural Restricted Precursors. ,
Stem Cells
 30 , 1265 , 2012
Cells
Neuroshere
Enzymes
Trypsin : 0.25%
Papain : 100 u
Medium
Pro-N

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.