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Tissue References Search Tool

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Neural
Mouse
Mouse
Panchision David M, Chen Hui-Ling, Pistollato Francesca, Papini Daniela, Ni Hsiao-Tzu, Hawley Teresa S.
Optimized flow cytometric analysis of central nervous system tissue reveals novel functional relationships among cells expressing CD133, CD15, and CD24 ,
Stem Cells
 25 , 1560-70 , 2007
Cells
Neural, various
Enzymes
Papain : 12 u/ml
Collagenase/Dispase : see reference
Trypsin : see reference
Medium
see reference
Neural
Rat
Rat, embryonic 14.5 day
Mirsky, R. and Jessen, K.
Isolation of Schwann Cell Precursors from Rodents. ,
Methods Mol Biol
 1739 , 3-15 , 2018
Cells
Schwann cell precursors
Enzymes
Medium
DMEM/ Ham's F-12
Neural
Rat
Rat, Wistar, E15
Moriya-Ito K, Osada T, Ishimatsu Y, Muramoto K, Kobayashi T, Ichikawa M.
Maturation of vomeronasal receptor neurons in vitro by coculture with accessory olfactory bulb neurons ,
Chem Senses
 30 , 111 , 2005
Cells
Vomeronasal receptor neurons
Enzymes
Papain : 0.5 u/ml
Medium
DMEM/F12
Pancreas
Guinea-Pig
Guinea-pig, Hartley, albino, male, 350-400 g
Gardner, J. , Conlon, T. , Klaeveman, H. , Adams, T. and Ondetti, M.
Action of Cholecystokinin and Cholinergic Agents on Calcium Transport in Isolated Pancreatic Acinar Cells ,
J Clin Invest
 56 , 366 , 1975
Cells
Acinar
Enzymes
Medium
Kreb's Ringer
Pancreas
Human
Human
Cane, M. , Sutton, R. and Criddle, D.
Isolation of Human Pancreatic Acinar Cells From Rat and Human Pancreas ,
Pancreapedia
 20 , 1 , 2011
Cells
Acinar
Enzymes
CLSPA : 200 u/ml
Medium
See Reference
Pancreas
Mouse
Mouse, adult, male
Ji, B. , Gaiser, S. , Chen, X. , Ernst, S. and Logsdon, C.
Intracellular Trypsin Induces Pancreatic Acinar Cell Death but not NF-KappaB Activation. ,
J Biol Chem
 284 , 17488 , 2009
Cells
Ancinar
Enzymes
CLSPA : see reference
Medium
DMEM
Pancreas
Mouse
Mouse, 6-10 month
Wang, Y. , Soyombo, A. , Shcheynikov, N. , Zeng, W. , Dorwart, M. , Marino, C. , Thomas, P. and Muallem, S.
Slc26a6 Regulates CFTR Activity In Vivo to Determine Pancreatic Duct HCO3- Secretion: Relevance to Cystic Fibrosis. ,
EMBO J
 25 , 5049 , 2006
Cells
Pancreatic ductal
Enzymes
CLSPA : 50 u/ml
Hyaluronidase : 400 u/ml
Medium
DMEM
Pancreas
Mouse
Mouse, male, 18-24 g
Burnham DB, Williams JA.
Effects of carbachol, cholecystokinin, and insulin on protein phosphorylation in isolated pancreatic acini ,
J Biol Chem
 257 , 10523-8 , 1982
Cells
Acinar
Enzymes
CLSPA : 70-90 u/ml
Medium
Krebs-Henseleit
Pancreas
Rat
Rat, SD, adult
Li, J. , Zhou, R. , Bie, B. , Huang, N. , Guo, Y. , Chen, H. , Shi, M. , Yang, J. , Zhang, J. and Li, Z.
Emodin and Baicalein Inhibit Sodium Taurocholate-Induced Vacuole Formation in Pancreatic Acinar Cells. ,
World J Gastroenterol
 24 , 35-45 , 2018
Cells
Acinar
Enzymes
Medium
Culture media
Pancreas
Rat
Rat, SD, male
Ji B, Kopin AS, Logsdon CD.
Species differences between rat and mouse CCKA receptors determine the divergent acinar cell response to the cholecystokinin analog JMV-180 ,
J Biol Chem
 275 , 19115-20 , 2000
Cells
Ancinar
Enzymes
CLSPA : see reference
Medium
DMEM
Pancreas
Rat
Rat
Blinman TA, Gukovsky I, Mouria M, Zaninovic V, Livingston E, Pandol SJ, Gukovskaya AS.
Activation of pancreatic acinar cells on isolation from tissue: cytokine upregulation via p38 MAP kinase ,
Am J Physiol Cell Physiol
 279 , C1993-2003 , 2000
Cells
Pancreatic acini
Enzymes
CLSPA : 30 u/ml
Collagenase Type 4 : 30 u/ml
Medium
M199
Pancreas
Rat
Rat, SD, male, 150 - 200 g
Menozzi, D. , Sato, S. , Jensen, R. and Gardner, J.
Cyclic GMP Does Not Inhibit Protein Kinase C-Mediated Enzyme Secretion in Rat Pancreatic Acini ,
J Biol Chem
 264 , 995 , 1989
Cells
Acinar
Enzymes
Medium
HEPES
Pituitary
Mouse
Mouse
Pyczek, J. , Buslei, R. , Schult, D. , Holsken, A. , Buchfelder, M. , Hess, I. , Hahn, H. and Uhmann, A.
Hedgehog Signaling Activation Induces Stem Cell Proliferation and Hormone Release in the Adult Pituitary Gland. ,
Sci Rep
 6 , 24928 , 2016
Cells
Pituitary
Enzymes
Medium
DMEM
Skin
Mouse
Mouse
King, I. , Kroenke, M. and Segal, B.
GM-CSF-Dependent, CD103+ Dermal Dendritic Cells Play a Critical Role in Th Effector Cell Differentiation After Subcutaneous Immunization. ,
J Exp Med
 207 , 953 , 2010
Cells
Epidermal and dermal
Enzymes
Medium
PBS
Skin
Mouse
Mouse, 3-7 month
Bradshaw AD, Francki A, Motamed K, Howe C, Sage EH.
Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation ,
Mol Biol Cell
 10 , 1569-79 , 1999
Cells
Fibroblasts, mesangial, smooth muscle
Enzymes
Trypsin : 0.25%
Collagenase : see reference
Medium
DMEM
Spleen
Mouse

Mouse

Comeau, K. , Caillon, A. , Paradis, P. and Schiffrin, E.
Determination of Interleukin-17A and Interferon-γ Production in γδ, CD4, and CD8 T Cells Isolated from Murine Lymphoid Organs, Perivascular Adipose Tissue, Kidney, and Lung. ,
Bio Protoc
 13 , e4679 , 2023
Cells
T cells
Enzymes
Medium
RPMI

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.