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Tissue References Search Tool

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Pancreas
Rat
Rat
Takaki, R. and Ono J
Culture of Pancreatic Islet Cells , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12B:11.1 , 1995
Cells
Islets
Enzymes
Collagenase : 0.5 - 0.9%
Medium
HBSS
Pancreas
Rat
Rat
Githens, S.
Pancreatic Duct Epithelial Cells , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12B:12.1 , 1995
Cells
Duct
Enzymes
Papain : 25 u/ml
Medium
DMEM /F-12
Pancreas
Rat
Rat, S-Wistar, male, 230 - 270 g
(also mouse, 6 - 8 wk old)
Ohzato, H. , Gotoh, M. , Monden, M. , Dono, K. , Kanai, T. and Mori, T.
Improvement in the Islet Yield From a Cold-Preserved Pancreas by Pancreatic Ductal Collagenase Distention at the Time of Harvesting ,
Transplantation
 51 , 566 , 1991
Cells
Islets
Enzymes
Collagenase : 0.1% - 0.2%
Medium
HBSS
Pancreas
Rat
Rat, SD, both sexes, 6-20 wks old
Githens, S. , Schexnayder, J. , Desai, K. and Patke, C.
Rat Pancreatic Interlobular Duct Epithelium: Isolation and Culture in Collagen Gel ,
In Vitro Cell Dev Biol
 25 (8) , 679 , 1989
Cells
Interlobular ducts
Enzymes
Papain : 25 u/ml
Medium
DMEM/Ham's F-12
Pancreas
Rat
Rat, 200-350 g
Gotoh, M. , Maki, T. , Satomi, S. , Porter, J. , Bonner-Weir, S. , O'Hara, C. and Monaco, A.
Reproducible High Yield of Rat Islets By Stationary In Vitro Digestion Following Pancreatic Ductal or Portal Venous Collagenase Injection ,
Transplantation
 43 (5) , 725 , 1987
Cells
Islets
Enzymes
Medium
HBSS
Pancreas
Rat
Rat, Dark Agouti and Lewis rat donors, adult
Sutton, R. , Peters, M. , McShane, P. , Gray, D. and Morris, P.
An Improved Method for the Isolation of Islets of Langerhans From the Adult Rat Pancreas ,
Transplant Proc
 XVII (6) , 1819 , 1986
Cells
Islets
Enzymes
Medium
Hank's solution
Pancreas
Rat
Rat, Wistar, male
Katada, T. and Ui, M.
Enhanced Insulin Secretion and Cyclic Amp Accumulation in Pancreatic Islets Due to Activation of Native Calcium Ionophores ,
J Biol Chem
 254 (2) , 469 , 1979
Cells
Islets
Enzymes
Medium
Medium 199
Pancreas
Rat
Rat, Wistar, male, 300-350 g
Wolters, G. , Konijenendijk, W. and Bouman, P.
Effects of Fasting on Insulin Secretion, Islet Glucose Metabolism, and the Cyclic Adenosine 3'5'-Monophosphate Content of Rat Pancreatic Islets In Vitro ,
Diabetes
 26 (6) , 530 , 1977
Cells
Islets
Enzymes
Collagenase : 0.2%
Medium
Kreb's Ringer bicarbonate buffer
Pancreas
Rat
Rat, Wistar, male, 200 - 300 g
Shibata, A. , Ludvigsen, C. , Naber, S. , McKaneil M. and Lacy, P.
Standardization of a Digestion-Filtration Method for Isolation of Islets ,
Diabetes
 25 , 667 , 1976
Cells
Islets
Enzymes
Medium
HBSS
Pancreas
Rat
Rat, SD, albino, male, 150-200 g
Mangos, J. , McSherry, N. , Butcher, F. , Irwin, K. and Barber, T.
Dispersed Rat Parotid Acinar Cells (Morphological, Functional Character) ,
Am J Physiol
 229 (3) , 560 , 1975
Cells
Acinar, parotid
Enzymes
Trypsin : 0.01%
Collagenase : 40-50 u/ml
Hyaluronidase : 0.10%
Medium
HBSS CMF
Pancreas
Rat
Rat, Wistar-Lewis, fetal 18-20 day gestation
(also neonatal 3-12 day old)
Braaten, J. , Jarlfors, U. , Smith, D. and Mintz, D.
Purification of Monolayer Cell Cultures of the Endocrine Pancreas ,
Tissue Cell
 7 (4) , 747 , 1975
Cells
Islets
Enzymes
Medium
EBSS
(see reference)
Pancreas
Rat
Rat, male, albino, 200-300 g
Lacy, P. , Walker, M. and Fink, J.
Perifusion of Isolated Rat Islets in Vitro ,
Diabetes
 21 (10) , 987 , 1972
Cells
Islets
Enzymes
Collagenase : 0.5%
Medium
Hanks solution
Pancreas
Rat
Rat, Lewis, inbred
Ballinger, W. and Lacy, P.
Transplantation of Intact Pancreatic Islets in Rats ,
Surgery
 72 (2) , 175 , 1972
Cells
Islets
Enzymes
Collagenase : 0.5%
Medium
HBSS
Pancreas
Rat
Rat, Wistar, albino, male, 400 - 500 g
Lacy, P. and Kostianovsky, M.
Method for the Isolation of Intact Islets of Langerhans from the Rat Pancreas ,
Diabetes
 16 , 35 , 1967
Cells
Islets
Enzymes
Collagenase Type 4 : 1.0% - 1.2%
Medium
HBSS
Parotid
Mouse
Mouse
Ogawa, M. , Oshima, M. , Imamura, A. , Sekine, Y. , Ishida, K. , Yamashita, K. , Nakajima, K. , Hirayama, M. , Tachikawa, T. and Tsuji, T.
Functional Salivary Gland Regeneration by Transplantation of a Bioengineered Organ Germ. ,
Nat Commun
 4 , 2498 , 2013
Cells
Parotid and sublingual glandular
Enzymes
Neutral Protease : 50 u/ml
Collagenase Type 1 : 100 u/ml
Medium
PBS
Parotid
Rat
Rat, Wistar, male, 130 g
Looms, D. , Dissing, S. , Tritsaris, K. , Pedersen, A. and Nauntofte, B.
Adrenoceptor-Activated Nitric Oxide Synthesis in Salivary Acinar Cells. ,
Adv Dent Res
 14 , 62 , 2000
Cells
Acinar
Enzymes
Collagenase : 75 u/ml
Hyaluronidase : 153 u/ml
Medium
RPMI 1640
Parotid
Rat
Rat, SD, 100-120 g
D'Silva NJ, DiJulio DH, Belton CM, Jacobson KL, Watson EL.
Immunolocalization of Rap1 in the rat parotid gland: detection on secretory granule membranes ,
J Histochem Cytochem
 45 , 965-73 , 1997
Cells
Parotid acinar cells
Enzymes
Collagenase Type 2 : 90 u/ml
Medium
Krebs-Henseleit Bicarbonate
Parotid
Rat
Rat, SD, male
Prasad, K.N. , Edwards-Prasad, J. , Carvaiho, I. , LaRosa, F.G. , Baibinder, E. , Meyers, A. and Quissell, D.
Establishment of Primary Cultures of Rat and Human Parotid Epithelial Cells for Transfection Experiments ,
In Vitro Cell Dev Biol
 28 , 493 , 1992
Cells
Epithelial
Enzymes
Collagenase : 50-75 u/ml
Hyaluronidase : 0.1%
Medium
HBSS CF
Parotid
Rat
Rat, SD, albino, male, 150-200 g
Mangos, J. , McSherry, N. , Butcher, F. , Irwin, K. and Barber, T.
Dispersed Rat Parotid Acinar Cells (Morphological, Functional Character) ,
Am J Physiol
 229 (3) , 560 , 1975
Cells
Acinar, parotid
Enzymes
Trypsin : 0.01%
Collagenase : 40-50 u/ml
Hyaluronidase : 0.10%
Medium
HBSS CMF
Pituitary
Bovine
Calf, male, less then 6 months old
Hassan, H. and Merkel, R.
Perifusion Model System to Culture Bovine Hypothalamic Slices In Series with Dispersed Anterior Pituitary Cells ,
In Vitro Cell Dev Biol
 30A , 435 , 1994
Cells
Pituitary
Enzymes
Collagenase : 0.3%
Medium
DMEM
Pituitary
Bovine
Bovine
Mason, W. and Ingram, C.
Techniques for Studying the Role of Electrical Activity in Control of Secretion by Normal Anterior Pituitary Cells , Vol. 124 , 207 , 1986
Cells
Pituitary
Enzymes
Collagenase : 0.1%
Medium
EBSS, CMF
Pituitary
Mouse
Mouse
Perez Millain, M. , Brinkmeier, M. , Mortensen, A. and Camper, S.
PROP1 Triggers Epithelial-Mesenchymal Transition-Like Process in Pituitary Stem Cells. ,
Elife
 5 , 2016
Cells
Pituitary
Enzymes
Medium
HBSS
Pituitary
Mouse
Mouse
Pyczek, J. , Buslei, R. , Schult, D. , Holsken, A. , Buchfelder, M. , Hess, I. , Hahn, H. and Uhmann, A.
Hedgehog Signaling Activation Induces Stem Cell Proliferation and Hormone Release in the Adult Pituitary Gland. ,
Sci Rep
 6 , 24928 , 2016
Cells
Pituitary
Enzymes
Medium
DMEM
Pituitary
Mouse
Mouse, male
Steveson Tami C, Ciccotosto Giuseppe D, Ma Xin-Ming, Mueller Gregory P, Mains Richard E, Eipper Betty A.
Menkes protein contributes to the function of peptidylglycine alpha-amidating monooxygenase ,
Endocrinology
 144 , 188-200 , 2003
Cells
Pituitary
Enzymes
Collagenase : 0.4%
Hyaluronidase : 0.1%
Trypsin : 0.3%
Medium
DMEM/Han's F12
Pituitary
Ovine
Ovine, adult
Xu Ruwei, Wang Qinling, Yan Ming, Hernandez Maria, Gong Changhong, Boon WahChin, Murata Yoko, Ueta Yoichi, Chen Chen.
Orexin-A augments voltage-gated Ca2+ currents and synergistically increases growth hormone (GH) secretion with GH-releasing hormone in primary cultured ovine somatotropes ,
Endocrinology
 143 , 4609-19 , 2002
Cells
Somatotropes
Enzymes
Medium
Medium 199
Pituitary
Rat
Rat, male, 12-15 week
Akieda-Asai, S. , Zaima, N. , Ikegami, K. , Kahyo, T, Yao, I. , Hatanaka, T. , Iemura, S. , Sugiyama, R. , Yokozeki, T. , Eishi, Y. , Koike, M. , Ikeda, K. , Chiba, T. , Yamaza, H. , Shimokawa, I. , Song, S. , Matsuno, A. , Mizutani, A. , Sawabe, M. Chao, N. and Tanaka, M.
SIRT1 Regulates Thyroid-Stimulating Hormone Release by Enhancing PIP5K gamma Activity through Deacetylation of Specific Lysine Residues in Mammals. ,
PLoS ONE
 5 , e11755 , 2010
Cells
Pituitary
Enzymes
Medium
DMEM
Prostate
Human
Human
Taylor, R. , Toivanen, R. , Frydenberg, M. , Pedersen, J. , Harewood, L. , Collins, A. , Maitland, N. and Risbridger, G.
Human Epithelial Basal Cells are Cells of Origin of Prostate Cancer, Independent of CD133 Status. ,
Stem Cells
 30 , 1087 , 2012
Cells
Prostatic cancer fibroblasts
Enzymes
Collagenase : 225 u/ml
Hyaluronidase : 125 u/ml
Medium
RPMI 1640
Prostate
Human
Human, 52-56 yr
Le Hanh, Arnold Julia T, McFann Kimberly K, Blackman Marc R.
DHT and testosterone, but not DHEA or E2, differentially modulate IGF-I, IGFBP-2, and IGFBP-3 in human prostatic stromal cells ,
Am J Physiol/Endo
 290 , E952-60 , 2006
Cells
Prostatic stromal cells
Enzymes
Medium
DMEM/F-12
Prostate
Human
Human
Nakashiro Koh-Ichi, Hara Shingo, Shinohara Yuji, Oyasu Miho, Kawamata Hitoshi, Shintani Satoru, Hamakawa Hiroyuki, Oyasu Ryoichi.
Phenotypic switch from paracrine to autocrine role of hepatocyte growth factor in an androgen-independent human prostatic carcinoma cell line, CWR22R ,
Am J Pathol
 165 , 533-40 , 2004
Cells
Prostate stromal cells
Enzymes
Medium
RPMI 1640
Prostate
Human
Human, fetal
Levine AC, Liu XH, Greenberg PD, Eliashvili M, Schiff JD, Aaronson SA, Holland JF, Kirschenbaum A.
Androgens induce the expression of vascular endothelial growth factor in human fetal prostatic fibroblasts ,
Endocrinology
 139 , 4672-8 , 1998
Cells
Prostatic fibroblasts
Enzymes
Medium
DMEM/F12
Prostate
Mouse
Mouse, 6-8 week
Burger, P. , Gupta, R. , Xiong, X. , Ontiveros, C. , Salm, S. , Moscatelli, D. and Wilson, E.
High Aldehyde Dehydrogenase Activity: A Novel Functional Marker of Murine Prostate Stem/Progenitor Cells. ,
Stem Cells
 27 , 2220-8 , 2009
Cells
Prostate epithelial/stem
Enzymes
Trypsin : 0.05%
Medium
HBSS
Prostate
Mouse
Mouse, 2 week
Imamov Otabek, Morani Andrea, Shim Gil-Jin, Omoto Yoko, Thulin-Andersson Christina, Warner Margaret, Gustafsson Jan-Ake.
Estrogen receptor beta regulates epithelial cellular differentiation in the mouse ventral prostate ,
Proc Natl Acad Sci U S A
 101 , 9375-80 , 2004
Cells
Prostatic epithelial
Enzymes
Collagenase Type 3 : 170 u/ml
Medium
DMEM
Prostate
Mouse
Mouse, male
Dubey P, Wu H, Reiter RE, Witte ON.
Alternative pathways to prostate carcinoma activate prostate stem cell antigen expression ,
Cancer Res
 61 , 3256-61 , 2001
Cells
Prostatic stem
Enzymes
Collagenase Type 1 : 170 u/ml
Medium
DMEM
Reproductive
Bovine
Bovine
Levy N, Gordin M, Mamluk R, Yanagisawa M, Smith M F, Hampton J H, Meidan R.
Distinct cellular localization and regulation of endothelin-1 and endothelin-converting enzyme-1 expression in the bovine corpus luteum: implications for luteolysis ,
Endocrinology
 142 , 5254-60 , 2001
Cells
Corpus leuteal cells
Enzymes
Collagenase Type 4 : 420 u/ml
Medium
M-199
Reproductive
Bovine
Bovine, female
Tsang PC, Poff JP, Boulton EP, Condon WA.
Four-day-old bovine corpus luteum: progesterone production and identification of matrix metalloproteinase activity in vitro ,
Biol Reprod
 53 , 1160-8 , 1995
Cells
Leuteal
Enzymes
Medium
Ham's F-12
Reproductive
Bovine
Calf, mid to late gestational
Coplen, D. , Howard, P. , Duckett, J. , Snyder, H. and Macarak, E.
Characterization of a Fibroblast Cell From the Urinary Bladder Wall ,
In Vitro Cell Dev Biol
 30A , 604 , 1994
Cells
Fibroblasts
Enzymes
Collagenase : 0.1%
Medium
Medium 199

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.