Tissue References Search Tool

Mouse

Mouse, female

Hamilton-Easton A. and Eichelberger M

Virus-specific antigen presentation by different subsets of cells from lung and mediastinal lymph node tissues of influenza virus-infected mice ,
J Virol
69 , 6359 , 1995

Cells

Antigen presenting cells

Enzymes

Collagenase : 150 u/ml

Deoxyribonuclease I : 30 u/ml

Medium

RPMI 1640

Porcine

Swine fetuses (70 day), adult Amer Dutch 250 day
(also human fetuses, 80 day)

Hinz, R. and Syverton, J.

Mammalian Cell Cultures for Study of Influenza Virus. I. Preparation of Monolayer Cultures with Collagenase ,
Proc Soc Exp Biol Med
101 , 19 , 1959

Cells

Lung

Enzymes

Collagenase : 0.01%

Medium

HBSS

Rabbit

Rabbit, New Zealand, white, male, 1.7 Kg

Gould, M. , Clements, J. , Jones, A. and Felts, J.

Dispersal of Rabbit Lung into Individual Viable Cells: A New Model for the Study of Lung Metabolism ,
Science
178 , 1209 , 1972

Cells

Lung

Enzymes

Pronase : 0.2%

Medium

Kreb's serum substitute solution, CMF

Rat

Jones, R. , Capen, D. , Cohen, K. , Munn, L. , Jain, R. and Duda, D.

A Protocol for Phenotypic Detection and Characterization of Vascular Cells of Different Origins in a Lung Neovascularization Model in Rodents. ,
Nat Protoc
3 , 388-97 , 2008

Cells

Lung

Enzymes

Collagenase Type 2 : 0.2%

Medium

PBS

Rat

Rat, SD, 125-150 g

Liebler, J. , Borok, Z. , Li, X. , Zhou, B. , Sandoval, A. and Kim, K.and Crandall, E.

Alveolar Epithelial Type I Cells Express Beta 2-Adrenergic Receptors and G-protein Receptor Kinase 2. ,
J Histochem Cytochem
52 , 759 , 2004

Cells

Alveolar type I & II

Enzymes

Elastase : 2.5-8 u/ml

Collagenase Type 1 : 1.0%

Medium

DMEM/F12

Rat

Rat, SD, 300-400 g

King J, Hamil T, Creighton J, Wu S, Bhat P, McDonald F. and Stevens T

Structural and functional characteristics of lung macro- and microvascular endothelial cell phenotypes ,
Microvasc Res
67 , 139 , 2004

Cells

Pulmonary endothelial cells

Enzymes

Collagenase Type 2 : 1000 u/ml

Medium

DMEM/F-12

Rat

Rat, Lewis, male, 200-250g

Steinmuller C, Franke-Ullmann G, Lohmann-Matthes ML, Emmendorffer A.

Local activation of nonspecific defense against a respiratory model infection by application of interferon-gamma: comparison between rat alveolar and interstitial lung macrophages ,
Am J Respir Cell Mol Biol
22 , 481-90 , 2000

Cells

Interstitial lung macrophages

Enzymes

Collagenase Type 1 : 100 u/ml

Deoxyribonuclease I : 50 u/ml

Medium

RPMI-1640

Rat

Rat, male, 200-250g

Bakhramov, A, Evans, M. and Kozlowski, R

Differential effects of hypoxia on the intracellular Ca2+ concentration of myocytes isolated from different regions of the rat pulmonary arterial tree ,
Exp Physiol
83 , 337-47 , 1998

Cells

Pulmonary arterial myocytes

Enzymes

Collagenase : 0.15%

Papain : 0.15%

Elastase : 0.05%

Medium

PBS

Rat

Rat, adult

Douglas, W. and Kaighn, M.

Clonal Isolation of Differentiated Rat Lung Cells ,
In Vitro
10 , 230 , 1974

Cells

Lung

Enzymes

Collagenase : 0.1%

Medium

Moscona saline, CMF

Mouse

Terhorst, D. , Chelbi, R. , Wohn, C. , Malosse, C. , Tamoutounour, S. , Jorquera, A. , Bajenoff, M. , Dalod, M. , Malissen, B. and Henri, S.

Dynamics and Transcriptomics of Skin Dendritic Cells and Macrophages in an Imiquimod-Induced, Biphasic Mouse Model of Psoriasis. ,
J Immunol
195 , 4953-61 , 2015

Cells

Dendritic

Enzymes

Collagenase Type 4 : 0.1%

Deoxyribonuclease I : 0.1%

Medium

RPMI 1640

Mouse

Dubrot, J. , Duraes, F. , Potin, L. , Capotosti, F. , Brighouse, D. , Suter, T. , LeibundGut-Landmann, S. , Garbi, N. , Reith, W. , Swartz, M. and Hugues, S.

Lymph Node Stromal Cells Acquire Peptide-MHCII Complexes from Dendritic Cells and Induce Antigen-Specific CD4+T Cell Tolerance ,
J Exp Med
211 , 1153 , 2014

Cells

Stromal

Enzymes

Collagenase Type 4 : 0.1%

Deoxyribonuclease I : 0.004%

Medium

RPMI

Mouse

Rayamajhi, M. , Redente, E. , Condon, T. , Gonzalez-Juarrero, M. , Riches, D. and Lenz, L.

Non-Surgical Intratracheal Instillation of Mice with Analysis of Lungs and Lung Draining Lymph Nodes by Flow Cytometry. ,
J Vis Exp
51 , 2702 , 2011

Cells

Lung draining

Enzymes

Collagenase Type 4 : 0.1-0.125%

Medium

HBSS

Mouse

Kapasi ZF, Qin D, Kerr WG, Kosco-Vilbois MH, Shultz LD, Tew JG, Szakal AK.

Follicular dendritic cell (FDC) precursors in primary lymphoid tissues ,
J Immunol
160 , 1078-84 , 1998

Cells

Follicular dentritic

Enzymes

Collagenase Type 4 : 0.25%

Deoxyribonuclease I : 0.5%

Medium

HBSS

Bovine

Bovine, lactating Holstein

Miranda, S. , Wang, Y. , Purdie, N. , Osborne, V. , Coomber, B. and Cant, J.

Selenomethionine Stimulates Expression of Glutathione Peroxidase 1 and 3 and Growth of Bovine Mammary Epithelial Cells in Primary Culture. ,
J Dairy Sci
92 , 2670 , 2009

Cells

Mammary epithelial

Enzymes

Collagenase Type 3 : 400 u/ml

Hyaluronidase : 100 u/ml

Deoxyribonuclease I : 2 u/ml

Medium

HBSS

Bovine

Bovine, heifer, 200kg

Weber M. , Purup S. , Vestergaard M. , Ellis S. , Scndergard-Andersen J. , Akers R. and Sejrsen K.

Contribution of Insulin-like Growth Factor (IGF)-I and IGF-binding Protein-3 to Mitogenic Activity in Bovine Mammary Extracts and Serum ,
J Endocrinol
161 , 365 , 1999

Cells

Mammary epithelial

Enzymes

Collagenase Type 2 : 1%

Hyaluronidase : 1%

Deoxyribonuclease I : 0.03%

Medium

M-199

Bovine

Bovine, young, lactating, female

Anderson, C. and Larson, B.

Comparative Maintenance of Function in Dispersed Cell and Organ Cultures ,
Exp Cell Res
61 , 24 , 1970

Cells

Mammary

Enzymes

Collagenase : 0.30 %

Medium

HBSS or EBSS

Bovine

Bovine, dairy, purebred,
(also rat, Holtzmann, albino, white)

Schingoethe, D. , Hageman, E. and Larson, B.

Essential Amino Acids for Milk Protein Synthesis in the In Vitro Secretory Cell and Stimulation by Elevated Levels ,
Biochim Biophys Acta
148 , 469 , 1967

Cells

Secretory

Mammary gland

Enzymes

Collagenase : 0.02 - 0.03%

Medium

HBSS or EBSS

Canine

Canine, adult

Petrouškova, P. , Hudáková, N. , Almášiová, V. , Valenčáková, A. , Horňáková, L. , Huniadi, M. and Čížková, D.

Establishment of Primary Cell Cultures from Canine Mammary Gland Malignant Tumours: A Preliminary Study. , J Vet Res Vol. 69 , 159-168 , 2025

Cells

Mammary gland tumor

Enzymes

Collagenase Type 4 : 0.05%

Medium

EBSS

Goat

Goat, lactating, 1 month

Blanco, A. , Rife, U. and Larson, B.

Lactate Dehydrogenase Isozymes during Dedifferentiation in Cultures of Mammary Secretory Cells ,
Nature
214 , 1331 , 1967

Cells

Mammary gland

Enzymes

Collagenase : 0.02 - 0.03%

Medium

HBSS or EBSS

Human

Labarge, M. , Garbe, J. and Stampfer, M.

Processing of Human Reduction Mammoplasty and Mastectomy Tissues for Cell Culture. ,
J Vis Exp
71 , 2013

Cells

Mammary epithelial

Enzymes

Collagenase : 200 u/ml

Hyaluronidase : 100 u/ml

Medium

DMEM/F-12

Human

Human, normal biopsy

Ronnov-Jessen L. , Villadsen R. , Edwards J. and Petersen O.

Differential Expression of a Chloride Intracellular Channel Gene, CLIC4, in Transforming Growth Factor-beta1-mediated Conversion of Fibroblasts to Myofibroblasts ,
Am J Pathol
161 , 471 , 2002

Cells

Fibroblasts

Enzymes

Collagenase Type 3 : 900 u/ml

Medium

DMEM/F-12

Human

Huss, F. and Kratz, G.

Mammary Epithelial Cell and Adipocyte Co-Culture in a 3-D Matrix: The First Step Towards Tissue-Engineered Human Breast Tissue. ,
Cells Tissues Organs
169 , 361-7 , 2001

Cells

Mammary epithelial cells

Enzymes

Collagenase Type 1 : 0.1%

Medium

DMEM

Human

Ogmundsdottir, H. , Petursdottir, I. , Gudmundsdottir, I. , Amundadottir, L. , Ronnov-Jessen, L. and Petersen, O.

Effects of Lymphocytes and Fibroblasts on the Growth of Human Mammary Carcinoma Cells Studied in Short-Term Primary Cultures ,
In Vitro Cell Dev Biol
29A , 936 , 1993

Cells

Epithelial

Fibroblasts

Enzymes

Collagenase Type 1 : 450 IU/ml

Medium

DMEM/Ham's F-12

Human

Ronnov-Jessen, L. , VanDeurs, B. , Nielsen, M. and Petersen, O.W.

Identification, Paracrine Generation, and Possible Function of Human Breast Carcinoma Myofibroblasts in Culture ,
In Vitro Cell Dev Biol
28 , 273 , 1992

Cells

Myofibroblasts

Enzymes

Collagenase : 900 IU/ml

Medium

DME - F12

Human

Emerman, J. and Wilkinson, D.

Routine Culturing of Normal, Dysplastic and Malignant Human Mammary Epithelial Cells from Small Tissue Samples ,
In Vitro Cell Dev Biol
26 , 1186 , 1990

Cells

Epithelial

Enzymes

Collagenase : 2.0%

Medium

DMEM/Ham's F-12

Mouse

Mouse, female

Sun, H. , Xu, X. and Deng, C.

Preparation of Single Epithelial Cells Suspension from Mouse Mammary Glands. ,
Bio Protoc
10 , e3530 , 2020

Cells

Mammary epithelial

Enzymes

Collagenase Type 3 : 300 u/ml

Hyaluronidase : 100 u/ml

Neutral Protease : 0.5%

Deoxyribonuclease I : 0.001%

Medium

DMEM/F-12

Mouse

Liu, X. , Johnson, S. , Liu, S. , Kanojia, D. , Yue, W. , Singh, U. , Wang, Q, Wang Qi, Nie, Q. and Chen H.

Nonlinear Growth Kinetics of Breast Cancer Stem Cells: Implications for Cancer Stem Cell Targeted Therapy. ,
Sci Rep
3 , 2473 , 2013

Cells

Mammary tumor

Enzymes

Collagenase : 0.15%

Hyaluronidase : 0.020%

Medium

DMEM/F12

Mouse

Mouse, 10 week

Cheng, N. and Lambert, D.

Mammary Transplantation of Stromal Cells and Carcinoma Cells in C57BL/6J Mice. ,
J Vis Exp
54 , e2716 , 2011

Cells

Fibroblasts, carcinoma

Enzymes

Collagenase Type 4 : 0.5%

Trypsin : 0.2%

Deoxyribonuclease I : 0.004

Hyaluronidase : 1,000 u/ml

Medium

DMEM

Mouse

Mouse, 12 week

Taddei, I. , Deugnier, M. , Faraldo, M. , Petit, V. , Bouvard, D. , Medina, D. , Fassler, R. , Thiery, J. and Glukhova, M.

Beta1 Integrin Deletion from the Basal Compartment of the Mammary Epithelium Affects Stem Cells ,
Nat Cell Biol
10 , 716-22 , 2008

Cells

Mammary epithelial

Enzymes

Collagenase : 0.3%

Hyaluronidase : 100 u/ml

Trypsin : 0.25%

Neutral Protease : 0.5%

Deoxyribonuclease I : 0.01%

Medium

see reference

Mouse

Mouse, 11 week

Mueller, S. , Clark, J. , Myers, P. and Korach, K.

Mammary Gland Development in Adult Mice Requires Epithelial and Stromal Estrogen Receptor Alpha. ,
Endocrinology
143 , 2357 , 2002

Cells

Epithelial

Enzymes

Collagenase Type 3 : 25 u/ml

Hyaluronidase : 0.1%

Protease XIV : 0.05%

Deoxyribonuclease I : 0.04%

Medium

DMEM/F12

Mouse

Boulanger CA, Smith GH.

Reducing mammary cancer risk through premature stem cell senescence ,
Oncogene
20 , 2264-72 , 2001

Cells

Mammary epithelial stem

Enzymes

Collagenase Type 3 : 0.1%

Hyaluronidase : 0.1%

Pronase : 1.25%

Deoxyribonuclease I : 0.2%

Medium

DMEM

Mouse

Mouse, BALB/C

Kanazawa, T. and Hosick, H.

Transformed Growth Phenotype of Mouse Mammary Epithelium in Primary Culture Induced by Specific Fetal Mesenchymes ,
J Cell Physiol
153 , 381 , 1992

Cells

Epithelium

Enzymes

Collagenase : 250 u/ml

Medium

HBSS

Mouse

Mouse (BALB/cCrgl), mature

Bandyopadhyay, G. , Imagawa, W. , Wallace, D. and Nandi, S.

Proliferative effects of insulin and epidermal growth factor on mouse mammary epithelial cells in primary culture ,
J Biol Chem
263 , 7567 , 1988

Cells

Epithelial

Enzymes

Pronase : 0.01%

Medium

Hepes buffered medium 199

Mouse

Mouse, BALB/c, female, 6-8 week

Ehmann, U. , Peterson, W. and Misfeldt, D.

To Grow Mouse Mammary Epithelial Cells in Culture ,
J Cell Biol
98 , 1026 , 1984

Cells

Epithelial

Enzymes

Collagenase Type 3 : 0.1%

Medium

DMEM