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Tissue References Search Tool

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Muscle
Bovine
Bovine
Lee, E. , Choi, J. , Hyun, J. , Cho, K. , Hwang, I. , Lee, H. , Chang, J. and Choi, I.
Steroid Effects on Cell Proliferation, Differentiation and Steroid Receptor Gene Expression in Adult Bovine Satellite Cells. ,
AJAS
 20 , 501 , 2007
Cells
Muscle satellite cells
Enzymes
Collagenase Type 2 : 300 u/ml
Medium
Krebs-Ringer Bicarbonate
Muscle
Bovine
Bovine
Yu, M. , McAndrew, R. , Al-Saghir, R. , Maier, K. , Medhora, M. , Roman, R. and Jacobs, E.
Nitric Oxide Contributes to 20-HETE-Induced Relaxation of Pulmonary Arteries. ,
J Appl Physiol
 93 , 1391 , 2002
Cells
Pulmonary artery endothelial and smooth muscle cells
Enzymes
Collagenase : 0.04-0.05%
Medium
RPMI-1640
Muscle
Bovine
Bovine
Absher, M. , Woodcock-Mitchell, J. , Mitchell, J. , Baldor, L. , Low, R. and Warshaw, D.
Characterization of Vascular Smooth Muscle Cell Phenotype in Long-Term Culture ,
In Vitro Cell Dev Biol
 25 (2) , 183 , 1989
Cells
Smooth muscle
Enzymes
Trypsin : 0.25%
Medium
DMEM
Muscle
Bovine
Bovine
Davies, P. and Kerr, C.
Modification of LDL Metabolism by Growth Factors in Cultured Vascular Cells and Human Skin Fibroblasts ,
Biochim Biophys Acta
 712 , 26 , 1982
Cells
Smooth muscle, fibroblasts
Enzymes
Trypsin : 0.055%
Medium
DMEM
Muscle
Canine
Canine
Parker, M. , Loretz, C. , Tyler, A. , Snider, L. , Storb. R. and Tapscott, S.
Inhibition of CD26/DPP-IV Enhances Donor Muscle Cell Engraftment and Stimulates Sustained Donor Cell Proliferation. ,
Skelet Muscle
 2 , 4 , 2012
Cells
Skeletal muscle
Enzymes
Collagenase Type 4 : 200 u/ml
Neutral Protease : 1 u/ml
Medium
DMEM
Muscle
Chicken
Chicken, 1-2 day
Dirksen W. , Vladic F. and Fisher S.
A Myosin Phosphatase Targeting Subunit Isoform Transition Defines a Smooth Muscle Developmental Phenotypic Switch ,
Am J Physiol/Cell
 278(3) , C589 , 2000
Cells
Gizzard and aorta smooth muscle
Enzymes
Medium
HBSS
Muscle
Chicken
Chick
Chamley-Campbell, J. , Campbell, G. and Ross, R.
The Smooth Muscle in Cell Culture ,
Physiol Res
 59 , 1 , 1979
Cells
Smooth muscle
Enzymes
Trypsin : 0.05% - 0.1%
Medium
HBSS
Muscle
Chicken
Chick, white leghorn, 12 day
Bullaro, J. and Brookman, D.
Comparison of Skeletal Muscle Monolayer Cultures Initiated With Cells Dissociated by the Vortex and Trypsin Methods ,
In Vitro
 12 , 564 , 1976
Cells
Muscle
Enzymes
Trypsin : 0.25%
Medium
Puck's saline A
Muscle
Chicken
Chick, white leghorn, embryos, 11 day
Tepperman, K. , Morris, G. , Essien, F. and Heywood, S.M.
A Mechanical Dissociation Method For Preparation of Muscle Cell Cultures ,
J Cell Physiol
 86 , 561 , 1975
Cells
Muscle
Enzymes
Trypsin : 0.05%
Medium
Saline G
Muscle
Chicken
Chick embryo
Hilfer, S. and Brown, J.
Collagenase. Its Effectiveness as a Dispersing Agent for Embryonic Chick Thyroid and Heart ,
Exp Cell Res
 65 , 246 , 1971
Cells
Thyroid
Muscle
Heart
Enzymes
Collagenase : 0.25%
Medium
Tyrode's saline, potassium free
Muscle
Chicken
Chick, embryonic
Hilfer, S.
Collagenase Treatment of Chick Heart and Thyroid , Tissue Cult Methods & Applications , Kruse, P., and Patterson, M. , 246 , 1971
Cells
Muscle
Enzymes
Trypsin : 0.1%
Medium
CMF HBSS
Muscle
Chicken
Chick embryonic
Rinaldini, L.
An Improved Method for the Isolation and Quantitative Cultivation of Embryonic Cells ,
Exp Cell Res
 16 , 477 , 1959
Cells
Various tissues (heart, liver, skeletal, cardiac)
Enzymes
Trypsin : various grades
Medium
CMF Tyrode's solution
Muscle
Feline
Cat, mongrel, adult, 2-4 kg
Follmer, C.H. , Ten Eick, R.E. and Yeh, J.Z.
Sodium Current Kinetics in Cat Atrial Myocytes ,
J Physiol
 384 , 169 , 1987
Cells
Myocytes
Enzymes
Collagenase : 0.12%
Medium
Kreb's Henseleit, CF
Muscle
Fish
Zebrafish
Alexander, M. , Kawahara, G. , Kho, A. , Howell, M. , Pusack, T. , Myers, J. , Montanaro, F. , Zon, L. , Guyon, J. and Kunkel, L.
Isolation and Transcriptome Analysis of Adult Zebrafish Cells Enriched for Skeletal Muscle Progenitors. ,
Muscle Nerve
 43 , 741-50 , 2011
Cells
Myogenic progenitor
Enzymes
Medium
PBS
Muscle
Frog
Frog, Xenopus laevis, embryos stage 17 & 19
Stollberg, J. and Fraser, S.
Acetylcholine Receptors and Con A-Binding Sites on Xenopus Muscle Cells ,
J Cell Biol
 107 , 1397 , 1988
Cells
Muscle
Enzymes
Collagenase : 0.10%
Medium
Steinberg's solution
Muscle
Frog
Frog
Shepherd, N. and Kavaler, F.
Direct Control of Contraction Force of Single Frog Atrial Cells by Extracellular Ions ,
Am J Physiol
 251 , C653 , 1986
Cells
Myocytes
Enzymes
Trypsin : 0.1%
Medium
CF Ringer
Muscle
Frog
Frog, Xenopus laevis
Anderson, M.J. , Cohen, M.W. and Zorychta, E.
Effects of Innervation on the Distribution of Acetylcholine Receptors on Cultured Muscle Cells ,
J Physiol
 268 , 731 , 1977
Cells
Muscle
Enzymes
Trypsin : 0.5%
Medium
L15 medium
(see reference)
Muscle
Guinea-Pig
Guinea-pig
Shieh CC, Feng J, Buckner SA, Brioni JD, Coghlan MJ, Sullivan JP, Gopalakrishnan M.
Functional implication of spare ATP-sensitive K(+) channels in bladder smooth muscle cells ,
J Pharmacol Exp Ther
 296 , 669-75 , 2001
Cells
Bladder smooth muscle
Enzymes
Collagenase Type 2 : 0.1-0.2%
Medium
Krebs-Ringer bicarbonate
Muscle
Guinea-Pig
Guinea-pig, 200-380 g
Schnitzler, M. , Derst, C. , Daut, J. and Preisig-Muller, R.
ATP-Sensitive Potassium Channels in Capillaries Isolated From Guinea-Pig Heart ,
J Physiol
 525 (2) , 307 , 2000
Cells
Capillaries
Myocytes
Enzymes
Medium
CF solution
Muscle
Guinea-Pig
Guinea-pig
(also rat, rabbit)
Hu, S. and Kim, H.
Activation of K+ Channel in Vascular Smooth Muscle by Cytochrome P-450 Metabolites of Arachidonic Acid ,
FASEB J
 6 , A383 , 1992
Cells
Smooth muscle
Enzymes
Trypsin : 0.1%
Medium
Potassium buffer solution
Muscle
Guinea-Pig
Guinea-pig, 200-300 g
Ohya, Y. and Sperelakis, N.
ATP Regulation of the Slow Calcium Channels in Vascular Smooth Muscle Cells ,
Circ Res
 64 , 145 , 1989
Cells
Smooth muscle
Mesenteric artery
Enzymes
Collagenase : 0.3%
Medium
CF solution
Muscle
Guinea-Pig
Guinea-pig, prepubertal
Ross, R.
The Smooth Muscle Cell . II. Growth of Smooth Muscle in Culture and Formation of Elastic Fibers ,
J Cell Biol
 50 , 172 , 1971
Cells
Smooth muscle
Aortic
Enzymes
Trypsin : 0.05%
Medium
Dulbecco-Vogt modification of Eagle's
Muscle
Hamster
Hamster, male, 60-70 day
Nakamura, T. , Iwata, Y. , Sampaolesi, M. , Hanada, H. , Saito, N. , Artman, M. , Coetzee, W. and Shigekawa, M.
Stretch-Activated Cation Channels in Skeletal Muscle Myotubes From Sarcoglycan-Deficient Hamsters ,
Am J Physiol Cell Physiol
 281 , C690 , 2001
Cells
Satellite
Enzymes
Trypsin : 0.25%
Medium
DMEM
Muscle
Human
Human
Malysz, J. , Rovner, E. , Wake, R. and Petkov, G.
Preparation and Utilization of Freshly Isolated Human Detrusor Smooth Muscle Cells for Characterization of 9-Phenanthrol-Sensitive Cation Currents. ,
J Vis Exp
 , 2020
Cells
Bladder smooth muscle
Enzymes
Collagenase Type 2 : 0.1-0.2%
Papain : 0.1-0.2%
Medium
Digestion solution (see reference)
Muscle
Human
Human
Garcia, S. , Tamaki, S. , Lee, S. , Wong, A. , Jose, A. , Dreux, J. , Kouklis, G. , Sbitany, H. , Seth, R. , Knott, P. , Heaton, C. , Ryan, W. , Kim, E. , Hansen, S. , Hoffman, W. and Pomerantz, J.
High-Yield Purification, Preservation, and Serial Transplantation of Human Satellite Cells. ,
Stem Cell Reports
 10 , 1160-1174 , 2018
Cells
Muscle satellite
Enzymes
Collagenase : 0.1%
Trypsin : 0.25%
Medium
DMEM
Muscle
Human
Human
Charville, G. , Cheung, T. , Yoo, B. , Santos, P. , Lee, G. , Shrager, J. and Rando, T.
Ex Vivo Expansion and In Vivo Self-Renewal of Human Muscle Stem Cells. ,
Stem Cell Reports
 5 , 621 , 2015
Cells
Skeletal muscle stem
Enzymes
Collagenase Type 2 : 750 u/ml
Neutral Protease : 2 u/ml
Medium
Ham's F10
Muscle
Human
Human
Lu, S. , Sun, X. , Hong, T. , Song, K. , Yang, S. and Wang, C.
Isolation and Culture of Smooth Muscle Cells from Human Acute Type A Aortic Dissection. ,
J Cardiothorac Surg
 8 , 83 , 2013
Cells
Smooth muscle
Enzymes
Medium
DMEM
Muscle
Human
Human, male
Stadler, G. , Chen, J. , Wagner, K. , Robin, J. , Shay, J. , Emerson, C. and Wright, W.
Establishment of Clonal Myogenic Cell Lines from Severely Affected Dystrophic Muscles - CDK4 Maintains the Myogenic Population. ,
Skelet Muscle
 1 , 12 , 2011
Cells
Myogenic
Enzymes
Medium
HBSS
Muscle
Human
Human
Nesti, L. , Jackson, W. , Shanti, R. , Koehler, S. , Aragon, A. , Bailey, J. , Sracic, M. , Freedman, B. , Giuliani, J. and Tuan, R.
Differentiation Potential of Multipotent Progenitor Cells Derived from War-Traumatized Muscle Tissue. ,
J Bone Joint Surg Am
 90 , 2390 , 2008
Cells
Muscle derived multiprogenitor cells
Enzymes
Medium
DMEM
Muscle
Human
Human
Moss, S. , Bates, M. , Parrino, P. and Woods, TC.
Isolation of Endothelial Cells and Vascular Smooth Muscle Cells from Internal Mammary Artery Tissue. ,
Ochsner J
 7 , 133 , 2007
Cells
Endothelial and vascular smooth muscle
Enzymes
Medium
HBSS
Muscle
Human
Human
Kimuli, M. , Eardley, I. and Southgate, J.
In Vitro Assessment of Decellularized Porcine Dermis as a Matrix for Urinary Tract Reconstruction. ,
BJU Int
 94 , 859 , 2004
Cells
Urinary tract smooth muscle
Enzymes
Collagenase Type 4 : 100 u/ml
Medium
DMEM
Muscle
Human
Human, fetal
(also bovine)
Davies, P. and Kerr, C.
Modification of LDL Metabolism by Growth Factors in Cultured Vascular Cells and Human Skin Fibroblasts ,
Biochim Biophys Acta
 712 , 26 , 1982
Cells
Smooth muscle, fibroblasts
Enzymes
Trypsin : 0.055%
Medium
DMEM
Muscle
Human
Human
Eskin, S. , Sybers, H. , Lester, J. , Navarro, L. , Gotto, A. and DeBakey, M.
Human Smooth Muscle Cells Cultured From Atherosclerotic Plaques and Uninvolved Vessel Wall ,
In Vitro
 17 (8) , 713 , 1981
Cells
Smooth muscle
Enzymes
Trypsin : 0.25%
Medium
DMEM
Muscle
Human
Human
(also rat, guinea-pig, chick, monkey)
Chamley-Campbell, J. , Campbell, G. and Ross, R.
The Smooth Muscle in Cell Culture ,
Physiol Res
 59 , 1 , 1979
Cells
Smooth muscle
Enzymes
Trypsin : 0.05% - 0.1%
Medium
HBSS
Muscle
Lizard
Lizard (Anolis carolinensis)
Cox, P. and Simpson, Jr., S.
A Microphotometric STudy of Myogenic Lizard Cells Grown in Vitro ,
Dev Biol
 23 , 433 , 1970
Cells
Myoblasts, tail
Enzymes
Collagenase : 0.2%
Medium
GM III
(see reference)
Muscle
Monkey
Monkey (Macaca nemestrina)
Chait, A. , Ross, R. , Albers, J. and Bierman, E.
Platelet-Derived Growth Factor Stimulates Activity of LDL Receptors ,
Proc Natl Acad Sci U S A
 77 , 4084 , 1980
Cells
Smooth muscle
Enzymes
Trypsin : 0.05%
Medium
Dulbecco-Vogt

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.