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Tissue References Search Tool

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Neural
Mouse
Mouse, adult
Weng, Y. , An, R. , Cassin, J. , Joseph, J. , Mi, R. , Wang, C. , Zhong, C. , Jin, S. , Pfeifer, G. , Bellacosa, A. , Dong, X. , Hoke, A. , He, Z. , Song, H. and Ming, G.
An Intrinsic Epigenetic Barrier for Functional Axon Regeneration ,
Neuron
 94 , 337 , 2018
Cells
Lumbar DRG
Enzymes
Collagenase Type 2 : 200 u/ml
Neutral Protease : 2.5 u/ml
Medium
HBSS
Neural
Mouse
Mouse, embryonic 12.5
Mirsky, R. and Jessen, K.
Isolation of Schwann Cell Precursors from Rodents. ,
Methods Mol Biol
 1739 , 3-15 , 2018
Cells
Schwann cell precursors
Enzymes
Medium
DMEM/ Ham's F-12
Neural
Mouse
Mouse, P2-P12
Fangmann, L. , Teller, S. , Stupakov, P. , Friess, H. , Ceyhan, G. and Demir, I.
3D Cancer Migration Assay with Schwann Cells. ,
Methods Mol Biol
 1739 , 317-325 , 2018
Cells
Dorsal root ganglia neurons
Enzymes
Medium
MEM
Neural
Mouse
Mouse
Xu, W. , Sachewsky, N. , Azimi, A. , Hung, M. , Gappasov, A. and Morshead, C.
Myelin Basic Protein Regulates Primitive and Definitive Neural Stem Cell Proliferation from the Adult Spinal Cord. ,
Stem Cells
 35 , 485-496 , 2017
Cells
Neurospheres
Enzymes
Trypsin : 0.13%
Hyaluronidase : 0.08%
Medium
Neurobasal A
Neural
Mouse
Mouse, day 2
Clements, M. , Byrne, E. , Camarillo Guerrero, L. , Cattin, A. , Zakka, L. , Ashraf, A. , Burden, J. , Khadayate, S. , Lloyd, A. , Marguerat, S. and Parrinello, S.
The Wound Microenvironment Reprograms Schwann Cells to Invasive Mesenchymal-like Cells to Drive Peripheral Nerve Regeneration. ,
Neuron
 96 , 98-114.e7 , 2017
Cells
Schwann
Enzymes
Medium
DMEM
Neural
Mouse
Mouse, 4 month
Tongtako, W. , Lehmbecker, A. , Wang, Y. , Hahn, K. , Baumgartner, W. and Gerhauser, I.
Canine Dorsal Root Ganglia Satellite Glial Cells Represent an Exceptional Cell Population with Astrocytic and Oligodendrocytic Properties. ,
Sci Rep
 7 , 13915 , 2017
Cells
Satellite glial
Enzymes
Medium
HBSS
Neural
Mouse
Mouse, 2-3 month
Sun, H. , Tsai, W. , Li, B. , Tao, W. , Chen, P. and Rubart, M.
Voltage-Induced Calcium Release in Postganglionic Sympathetic Neurons in Adult Mice. ,
PLoS ONE
 11 , e0148962 , 2016
Cells
Sympathetic neurons
Enzymes
Papain : 10 u/ml
Medium
EBSS
Neural
Mouse
Mouse, neonatal
Stettner, M. , Lohmann, B. , Wolffram, K. , Weinberger, J. , Dehmel, T. , Hartung, H. , Mausberg, A. and Kieseier, B.
Interleukin-17 Impedes Schwann Cell-Mediated Myelination. ,
J Neuroinflammation
 11 , 63 , 2014
Cells
Schwann cells
Enzymes
Collagenase : 0.05-0.1%
Trypsin : 0.125-0.25%
Medium
DMEM
Neural
Mouse
Mouse
Lee, B. , Cho, H. , Jung, J. , Yang, Y. , Yang, D. and Oh, U.
Anoctamin 1 Contributes to Inflammatory and Nerve-Injury Induced Hypersensitivity. ,
Mol Pain
 10 , 5 , 2014
Cells
DRG neurons
Enzymes
Trypsin : 0.25%
Medium
DMEM/F-12
Neural
Mouse
Mouse
Zhang, Y. and Hu, W.
Mouse Enteric Neuronal Cell Culture. ,
Methods Mol Biol
 1078 , 55-63 , 2013
Cells
Enteric neurons
Enzymes
Trypsin : 0.05%
Deoxyribonuclease I : 400 u/ml
Medium
DMEM/F12
Neural
Mouse
Mouse, 4-6 week
Masaki, T. , Qu, J. , Cholewa-Waclaw, J. , Burr, K. , Raaum, R. and Rambukkana, A.
Reprogramming Adult Schwann Cells to Stem Cell-Like Cells by Leprosy Bacilli Promotes Dissemination of Infection. ,
Cell
 152 , 51 , 2013
Cells
Schwann
Enzymes
Trypsin : 0.125%
Medium
DMEM
Neural
Mouse
Mouse, 6-8 week
Babona-Pilipos, R. , Popovic, M. and Morshead, C.
A Galvanotaxis Assay for Analysis of Neural Precursor Cell Migration Kinetics in an Externally Applied Direct Current Electric Field. ,
J Vis Exp
 68 , e4193 , 2012
Cells
Neural precursors
Enzymes
Trypsin : 0.13%
Hyaluronidase : 0.08%
Medium
see reference
Neural
Mouse
Mouse, P4-5
Ziegler, A. , Schneider, J. , Qin, M. , Tyler, W. , Pintar, J. , Fraidenraich, D. , Wood, T. and Levison, S.
IGF-II Promotes Stemness of Neural Restricted Precursors. ,
Stem Cells
 30 , 1265 , 2012
Cells
Neuroshere
Enzymes
Trypsin : 0.25%
Papain : 100 u
Medium
Pro-N
Neural
Mouse
Mouse
Salisbury, E. , Lazard, Z. , Ubogu, E. , Davis, A. and Olmsted-Davis, E.
Transient Brown Adipocyte-Like Cells Derive from Peripheral Nerve Progenitors in Response to Bone Morphogenetic Protein 2. ,
Stem Cells Transl Med
 1 , 874-85 , 2012
Cells
Nerve progenitors
Enzymes
Medium
HBSS
Neural
Mouse
Mouse, embryonic
Pfaltzgraff, E. , Mundell, N. and Labosky, P.
Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube. ,
J Vis Exp
 , e4134 , 2012
Cells
Neural crest
Enzymes
Medium
DMEM
Neural
Mouse
Mouse, embryonic
Conrad, R. , Jablonka, S. , Sczepan, T. , Sendtner, M. , Wiese, S. and Klausmeyer, A.
Lectin-Based Isolation and Culture of Mouse Embryonic Motoneurons. ,
J Vis Exp
 55 , e3200 , 2011
Cells
Motorneurons
Enzymes
Trypsin : 0.025%
Medium
HBSS
Neural
Mouse
Mouse, 4-8 week
Radtke, C. , Sasaki, M. , Lankford, K. , Gallo, V. and Kocsis, J.
CNPase Expression in Olfactory Ensheathing Cells. ,
J Biomed Biotechnol
 2011 , 608496 , 2011
Cells
Olfactory ensheathing
Enzymes
Collagenase : 0.15%
Papain : 12 u/ml
Medium
DMEM
Neural
Mouse
Mouse
Sayed, B. , Christy, A. , Walker, M. and Brown, M.
Meningeal Mast Cells Affect Early T Cell Central Nervous System Infiltration and Blood-Brain Barrier Integrity Through TNF: a Role for Neutrophil Recruitment? ,
J Immunol
 184 , 6891-900 , 2010
Cells
CNS leukocytes
Enzymes
Collagenase Type 4 : 300 u/ml
Medium
HBSS
Neural
Mouse
Mouse, embryonic
Radad Khaled, Gille Gabriele, Rausch Wolf-Dieter.
Dopaminergic neurons are preferentially sensitive to long-term rotenone toxicity in primary cell culture ,
Toxicol In Vitro
 22 , 68-74 , 2008
Cells
Dopaminergic neurons
Enzymes
Trypsin : 0.1%
Medium
DMEM
Neural
Mouse
Mouse, neonatal
Pedrola, L. , Espert, A. , Valdes-Sanchez, T. , Sanchez-Piris, M. , Sirkowski, E. , Scherer, S. , Farinas, I. and Palau, F.
Cell Expression of GDAP1 in the Nervous System and Pathogenesis of Charcot-Marie-Tooth Type 4A Disease. ,
J Cell Mol Med
 12 , 679 , 2008
Cells
DRG neurons
Enzymes
Collagenase : 0.2%
Trypsin : 0.05%
Medium
Ham's F12
Neural
Mouse
Mouse, 6-8 week
Malin, S. , Davis, B. and Molliver, D.
Production of Dissociated Sensory Neuron Cultures and Considerations for their use in Studying Neuronal Function and Plasticity ,
Nat Protoc
 2 , 152 , 2007
Cells
Sensory neurons, DRG
Enzymes
Papain : 20 u/ml
Medium
HBSS
Neural
Mouse
Mouse
Panchision David M, Chen Hui-Ling, Pistollato Francesca, Papini Daniela, Ni Hsiao-Tzu, Hawley Teresa S.
Optimized flow cytometric analysis of central nervous system tissue reveals novel functional relationships among cells expressing CD133, CD15, and CD24 ,
Stem Cells
 25 , 1560-70 , 2007
Cells
Neural, various
Enzymes
Papain : 12 u/ml
Collagenase/Dispase : see reference
Trypsin : see reference
Medium
see reference
Neural
Mouse
Mouse, adult
Gonzalez John M, Bergmann Cornelia C, Fuss Babette, Hinton David R, Kangas Cindy, Macklin Wendy B, Stohlman Stephen A.
Expression of a dominant negative IFN-gammareceptor on mouse oligodendrocytes ,
Glia
 51 , 22-34 , 2005
Cells
Brain and spinal cord cells
Enzymes
Trypsin : 0.25%
Medium
PBS
Neural
Mouse
Mouse, 1-3 day
Savchenko V, Sung U, Blakely RD.
Cell surface trafficking of the antidepressant-sensitive norepinephrine transporter revealed with an ectodomain antibody ,
Mol Cell Neurosci
 24 , 1131 , 2003
Cells
Neurons, ganglia
Enzymes
Papain : 20 u/ml
Deoxyribonuclease I : 100 u/ml
Collagenase : 0.3%
Trypsin : 0.05%
Medium
HBSS
Neural
Mouse
Mouse, postnatal-day-1-old (P1)
Deshmukh, M. , Kuida, K. and Johnson Jr., E.
Caspase Inhibition Extends the Commitment to Neuronal Death Beyond Cytochrome c Release to the Point of Mitochondrial Depolarization ,
J Cell Biol
 150 (1) , 131 , 2000
Cells
Neurons
Enzymes
Trypsin : 0.25%
Medium
NGF-containing medium
Neural
Mouse
Mouse embryos
Lee, K. , Davies, A. and Jaenisch, R.
P75-Eficient Embryonic Dorsal Root Sensory and Neonatal Sympathetic Neurons Display a Decreased Sensitivity to NGF ,
Development
 120 , 1027 , 1994
Cells
Neurons, DRG and SCG
Enzymes
Trypsin : 0.25%
Medium
L-15 medium
Neural
Mouse
mouse, 65 days
Richards L. , Kilpatrick T. and Bartlett P.
De Novo Generation of Neuronal Cells from the Adult Mouse Brain ,
Proc Natl Acad Sci U S A
 89 , 8591 , 1992
Cells
Neurons, neuronal precursors
Enzymes
Trypsin : 0.1%
Medium
DMEM
Neural
Mouse
Mouse, CD-1, neonate
Quinn, S. and De Boni, U.
Enhanced Neuronal Regeneration by Retinoic Acid of Murine Dorsal Root Ganglia and of Fetal Murine and human Spinal Cord in vitro ,
In Vitro Cell Dev Biol
 27 , 55 , 1991
Cells
Neurons, dorsal root ganglion
Enzymes
Trypsin : 0.25%
Medium
HBSS
Neural
Mouse
Mouse, fetal
Kitani, H. , Shiurba, R. , Sakakura, T. and Tomooka, Y.
Isolation and Characterization Of Mouse Neural Precursor Cells in Primary Culture ,
In Vitro Cell Dev Biol
 27 , 615 , 1991
Cells
Precursor
Enzymes
Trypsin : 0.5%
Medium
PBS
Neural
Mouse
Mouse, neonatal
(also chick)
Varon, S. , Skaper, S. , Barbin, G. , Selak, I. and Manthorpe, M.
Low Molecular Weight Agents Support Survival of Cultured Neurons From the Central Nervous System ,
J Neurosci
 4 (3) , 654 , 1984
Cells
PNS test neurons
Enzymes
Trypsin : 0.08%
Medium
Eagle's Basal Medium (see reference)
Neural
Mouse
Mouse (BALB/c), adult
Eagleson, K. and Bennett, M.
Survival of Purified Motor Neurons In Vitro: Effects of Skeletal Muscle-Conditioned Medium ,
Neurosci Lett
 38 , 187 , 1983
Cells
Neurons, spinal cord
Enzymes
Medium
Hank's BSS, CMF
Neural
Ovine
Lamb
(also calf)
Poduslo, S. , Miller, K. and McKhann, G.
Metabolic Properties of Maintained Oligodendroglia Purified from Brain ,
J Biol Chem
 253 , 1592 , 1978
Cells
Oligodendroglia
Neural
Enzymes
Trypsin : 0.1%
Medium
(see reference)
Neural
Porcine
Porcine, adult, 60-100 kg
Si ML, Lee TJ.
Presynaptic alpha7-nicotinic acetylcholine receptors mediate nicotine-induced nitric oxidergic neurogenic vasodilation in porcine basilar arteries ,
J Pharmacol Exp Ther
 298 , 122 , 2001
Cells
Superior cervical ganglia
Enzymes
Papain : 2 u/ml
Collagenase : 0.12%
Medium
HBSS
Neural
Quail
Quail
Sieber-Blum, M. and Cohen, A.
Clonal Analysis of Quail Neural Crest Cells: They are Pluripotent and Differentiate in Vitro in the Absence of Noncrest Cells ,
Dev Biol
 80 , 96 , 1980
Cells
Neural crest
Enzymes
Trypsin : 0.05%
Medium
MEM, HBSS
Neural
Rat
Rat, 2-3 week
Lin, Y. and Chen, J.
Dorsal Root Ganglia Isolation and Primary Culture to Study Neurotransmitter Release. ,
J Vis Exp
 , 2018
Cells
Dorsal root ganglia
Enzymes
Medium
HBSS
Neural
Rat
Rat, SD, 3 month
Andersen, N. and Monje, P.
Isolation, Culture, and Cryopreservation of Adult Rodent Schwann Cells Derived from Immediately Dissociated Teased Fibers. ,
Methods Mol Biol
 1739 , 49-66 , 2018
Cells
Schwann
Enzymes
Medium
DMEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.