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Tissue References Search Tool

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Adipose/Fat
Avian
Scherpelz, K. , Lu, J.,Tycko, R. and Meredith, S.
Preparation of Amyloid Fibrils Seeded from Brain and Meninges ,
Methods Mol Biol
 1345 , 299 , 2016
Cells
Amyloid Fibrils
Enzymes
Medium
50mM Tris
Adipose/Fat
Avian
Scherpelz, K. , Lu, J.,Tycko, R. and Meredith, S.
Preparation of Amyloid Fibrils Seeded from Brain and Meninges ,
Methods Mol Biol
 1345 , 299 , 2016
Cells
Amyloid Fibrils
Enzymes
Medium
50mM Tris
Epithelial
Chicken
Chick, 5 day old
Simon-Assmann, P. and Kedinger, M
Embryonic Gut-Dissagregated Culture , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12A:3.1 , 1995
Cells
Intestinal mesenchymal and epithelial
Enzymes
Collagenase : 0.03%
Medium
DMEM
Eye
Chicken
Chick embryo
Reenstra Wende R, Orlow Daniel L, Svoboda Kathy K H.
ECM-stimulated signaling and actin reorganization in embryonic corneal epithelia are Rho dependent ,
Inv Ophthal Visual Sci
 43 , 3181-9 , 2002
Cells
Corneal epithelia
Enzymes
Collagenase : 0.08%
Trypsin : 0.08%
Medium
HBSS
Heart
Chicken
Chicken, embryonic
Blech-Hermoni, Y. and Ladd, A.
Identification of Transcripts Regulated by CUG-BP, Elav-like Family Member 1 (CELF1) in Primary Embryonic Cardiomyocytes by RNA-seq. ,
Genom Data
 6 , 74-76 , 2015
Cells
Cardiomyocytes
Enzymes
Medium
HBSS
Kidney
Avian
Avian, house sparrows
Goldstein D. , Reddy V. and Plaga K.
Second Messenger Production in Avian Medullary Nephron Segments in Response to Peptide Hormones ,
Am J Physiol
 276 , R847 , 1999
Cells
Tubule segments
Enzymes
Medium
HBSS
Kidney
Avian
Avian, chicken
Reich, C. and Bonar, R.
Separation of Avian Kidney Tubules and Glomeruli for In Vitro Culture ,
Meth Cell Sci
 7 , 97 , 1982
Cells
Tubule and glomeruli
Enzymes
Medium
RPMI 1640
Liver
Avian
Avian, various
Else, P. , Brand, M. , Turner, N. and Hulbert, A
Respiration Rate of Hepatocytes Varies With Body Mass in Birds ,
J Exp Biol
 207 , 2305 , 2004
Cells
Hepatocytes
Enzymes
Medium
see reference
Liver
Avian
Ducklings, 7 day
Lee J. , Culvenor J. , Angus P. , Smallwood R. , Nicoll A. and Locarnini S.
Duck Hepatitis B Virus Replication in Primary Bile Duct Epithelial Cells ,
J Virol
 75(16) , 7651 , 2001
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Hyaluronidase : 0.05%
Medium
DMEM/ F12
Liver
Chicken
Chicken, adult
Fraslin, J. , Touquette, L. , Douaire, M. , Menezo, Y. , Guillemot, J. and Mallard, J.
Isolation and Long Term Maintenance of Differentiated Adult Chicken Hepatocytes in Primary Culture ,
In Vitro Cell Dev Biol
 28 , 615 , 1992
Cells
Hepatocytes
Enzymes
Collagenase : 0.02%
Medium
HEPES, CF
Liver
Chicken
Chicken
(also rat, SD, 150-250 g)
Weigel, P. , Schnaar, R. , Kuhlenschmidt, M. , Schmell, E. , Lee, R. , Lee, Y. and Roseman, S.
Adhesion of Hepatocytes to Immobilized Sugars ,
J Biol Chem
 254 (21) , 10830 , 1979
Cells
Hepatocytes
Enzymes
Collagenase Type 4 : 6000 units
Medium
HEPES
Muscle
Chicken
Chicken, 1-2 day
Dirksen W. , Vladic F. and Fisher S.
A Myosin Phosphatase Targeting Subunit Isoform Transition Defines a Smooth Muscle Developmental Phenotypic Switch ,
Am J Physiol/Cell
 278(3) , C589 , 2000
Cells
Gizzard and aorta smooth muscle
Enzymes
Medium
HBSS
Muscle
Chicken
Chick embryo
Hilfer, S. and Brown, J.
Collagenase. Its Effectiveness as a Dispersing Agent for Embryonic Chick Thyroid and Heart ,
Exp Cell Res
 65 , 246 , 1971
Cells
Thyroid
Muscle
Heart
Enzymes
Collagenase : 0.25%
Medium
Tyrode's saline, potassium free
Neural
Avian
Quail and Chicken, embryonic
Rollo, B. , Zhang, D. , Simkin, J. , Menheniott, T. and Newgreen, D.
Why are Enteric Ganglia so Small? Role of Differential Adhesion of Enteric Neurons and Enteric Neural Crest Cells. ,
F1000Res
 4 , 113 , 2015
Cells
Midgut DRG
Enzymes
Medium
Ham's F12
Neural
Avian
Chick, White Leghorn, embryos
Temburni, M. , Rosenberg, M. , Pathak, N. , McConnell, R. and Jacob, M.
Neuronal Nicotinic Synapse Assembly Requires the Adenomatous Polyposis Coli Tumor Suppressor Protein. ,
J Neurosci
 24 , 6776 , 2004
Cells
Cilary ganglion neurons
Enzymes
Medium
PBS
Neural
Chicken
Chick, White Leghorn, embryos
Watt, P. , Mullen, Y. , Benhamou, P. , Hober, C. , Nomura, Y. , Watanabe, Y. , Passaro, E. , Zinner, M. and Brunicardi, F.
Simplified Semiautomated Method For Isolating Islets From the Human Pancreas ,
Transplant Proc
 26 (2) , 582 , 1994
Cells
Islets
Enzymes
Trypsin : Trypsin: 0.125-0.25%
Collagenase : 0.2%
Deoxyribonuclease I : 200 u/ml
Medium
Eurocollins solution
Neural
Chicken
Chicken, embryos, 9-10 days old
Mudge, A. , Leeman, S. and Fischbach, G.
Enkephalin Inhibits Release of Substance P From Sensory Neurons in Culture and Decreases Action Potential Duration ,
Proc Natl Acad Sci U S A
 76 (1) , 526 , 1979
Cells
Dorsal root ganglia neurons
Enzymes
Collagenase : 0.01%
Medium
Eagle's MEM
Neural
Chicken
Chick, White Leghorn, embryos, 7 day
Wiseman, L. and Hammond, W.
The Reacquisition of Cell Adhesiveness Following Tissue Disaggregation by Eleven Different Agents ,
J Exp Zool
 197 , 429 , 1976
Cells
Neural retina
Enzymes
Collagenase : 0.25%
Elastase : 0.2%
Hyaluronidase : 1.0%
Papain : 1.0%
Protease : 0.1%
Trypsin : 0.05%
Medium
HBSS
Reproductive
Chicken

Chicken, male, 1 day

Huang, H. , Wang, M. , Hou, L. , Lin, X. , Pan, S. , Zheng, P. and Zhao, Q.
A Potential Mechanism Associated with Lead-induced Spermatogonia and Leydig Cell Toxicity and Mitigative Effect of Selenium in Chicken. , Ecotoxicol Environ Saf Vol. 209 , 111671 , 2021
Enzymes
Collagenase : 0.1%
Hyaluronidase : 0.1%
Trypsin : 0.1%
Medium
DMEM
Reproductive
Chicken
Chicken, Gallus Domesticus,20-30 week
Du Meihong, Han Haitang, Jiang Bin, Zhao Chen, Qian Changsong, Shen Haiyan, Xu Yan, Li Zandong.
An efficient isolation method for domestic hen (Gallus domesticus) ovarian primary follicles ,
J Reprod Dev
 52 , 569-76 , 2006
Cells
Primary follicles
Enzymes
Trypsin : 0.15%
Medium
Dulbecco's phosphate buffered saline
Thyroid/Parathyroid
Chicken
Chick embryo
Hilfer, S. and Brown, J.
Collagenase. Its Effectiveness as a Dispersing Agent for Embryonic Chick Thyroid and Heart ,
Exp Cell Res
 65 , 246 , 1971
Cells
Thyroid
Muscle
Heart
Enzymes
Collagenase : 0.25%
Medium
Tyrode's saline, potassium free
Thyroid/Parathyroid
Chicken
Chicken, Rhode Island Red, embryo
Spooner, B.
The Expression of Differentiation by Chick Embryo Throid in Cell Culture. I. Functional and Fine Structural Stability in Mass and Clonal Culture ,
J Cell Physiol
 75 , 33 , 1970
Cells
Thyroid follicular
Enzymes
Collagenase : 0.2%
Medium
Tyrode's solution, CMF

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.