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Tissue References Search Tool

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Mammary
Rat
Rat, female, 50 day
Maffini M. , Soto A. , Calabro J. , Ucci A. and Sonnenschein C.
The Stroma as a Crucial Target in Rat Mammary Gland Carcinogenesis ,
J Cell Sci
 117 , 1495 , 2004
Cells
Mammary epithelial
Enzymes
Medium
DMEM/F12
Mammary
Rat
Rat, female
Djuric Z. , Lewis S. , Mayhugh M. , Naegeli L. , Tang N. and Hart R.
Effect of Varying Caloric Restriction Levels on Female Rat Growth and 5-hydroxymethyl-2'-deoxyuridine in DNA ,
Toxicol Sci
 66 , 125 , 2002
Cells
Mammary gland epithelial
Enzymes
Medium
see reference
Mammary
Rat
Rat, SD
Brake P. , Zhang L. and Jefcoate C.
Aryl Hydrocarbon Receptor Regulation of Cytochrome P4501B1 in Rat Mammary Fibroblasts: Evidence for Transcriptional Repression by Glucocorticoids ,
Mol Pharmacol
 54 , 825 , 1998
Cells
Mammary fibroblasts
Enzymes
Medium
DMEM/F-12
Mammary
Rat
Rat, SD, 50 day
Varela L. , Darcy K. and Ip M.
The Epidermal Growth Factor Receptor is not Required for Tumor Necrosis Factor-alpha Action in Normal Mammary Epithelial Cells ,
Endocrinology
 138 , 3891 , 1997
Cells
Mammary epithelial
Enzymes
Medium
DMEM/F12
Mammary
Rat
Rat
(also human)
Soriano, J. , Pepper, M. , Nakamura, T. , Orci, L. and Montesano, R.
Hepatocyte Growth Factor Stimulates Extensive Development of Branching Duct-like Structures by Cloned Mammary Gland Epithelial Cells ,
J Cell Sci
 108 , 413-430 , 1995
Cells
Epithelial
Enzymes
Medium
DMEM
Mammary
Rat
Rat, SD, female, 60-90 day
Laduca, J. and Sinha, D.
In Vitro Carcinogenesis of Mammary Epithelial Cells by N-Nitroso-N-Methylurea Using a Collagen Gel Matrix Culture ,
In Vitro Cell Dev Biol
 29A , 789 , 1993
Cells
Epithelial
Enzymes
Medium
EBSS
Mammary
Rat
Rat, Lewis, female, 90 days
Lin, T. , Hom, Y.K. , Richards, J. and Nandi, S.
Effects of Antioxidants and Reduced Oxygen Tension on Rat Mammary Epithelial Cells in Culture ,
In Vitro Cell Dev Biol
 27A , 191 , 1991
Cells
Epithelial
Mammary
Enzymes
Collagenase : 0.05%
Medium
Medium 199
Mammary
Rat
Rat, LEW, virgin, female, 45-50 day
Ethler, S.
Primary Culture and Serial Passage of Normal and Carcinogen-Treated Rat Mammary Epithelial Cells In Vitro ,
J Natl Cancer Inst
 74 (6) , 1307 , 1985
Cells
Epithelial
Enzymes
Collagenase : 0.1%
Medium
Medium 199
Mammary
Rat
Rat, SD, female
Cohen, L.
Isolation and Characterization of a Serially Cultivated, Neoplastic, Epithelial Cell Line from the N-nitrosomethylurea Induced Rat Mammary Adenocarcinoma ,
In Vitro
 18 , 565 , 1982
Cells
Epithelial, cancer and tumor
Enzymes
Collagenase : 0.1%
Medium
Eagles's MEM
Mammary
Rat
Rat, inbred LEW, female, 50-60 day old
Richards, J. and Nandi, S.
Primary Culture of Rat Mammary Epithelial Cells. I. Effect of Plating Density, Horomones, and Serum on DNA Synthesis ,
J Natl Cancer Inst
 61 (3) , 765 , 1978
Cells
Epithelial
Enzymes
Medium
Medium 199
Mammary
Rat
Rat, Wistar, 13-18 day postpartum
Katz, J. , Wals, P. and Van de Velde, R
Lipogenesis by Acini from Mammary Gland of Lactating Rats ,
J Biol Chem
 249 , 7348 , 1974
Cells
Acini
Enzymes
Collagenase : 0.05%
Medium
HBSS
Mammary
Rat
Rat, SD, 15-20 day postpartum
Martin, R. and Baldwin, R.
Effects of Insulin on Isolated Rat Mammary Cell Metabolism: Glucose Utilization and Metabolite Patterns ,
Endocrinology
 89 , 1263 , 1971
Cells
Mammary
Enzymes
Collagenase : 0.2%
Medium
Kreb's Ringer bicarbonate buffer
Mammary
Rat
Rat, virgin
Moon, R. , Janns, D. and Young, S.
Preparation of Fat Cell-"Free" Rat Mammary Gland ,
J Histochem Cytochem
 17 (3) , 182 , 1969
Cells
Mammary
Enzymes
Collagenase : 0.35%
Medium
Medium 199
Miscellaneous
Rat
Rat, Lewis, M
Jain, S. , Tran, T. and Amiji, M.
Macrophage Repolarization with Targeted Alginate Nanoparticles Containing IL-10 Plasmid DNA for the Treatment of Experimental Arthritis. ,
Biomaterials
 61 , 162-77 , 2015
Cells
Synovial macrophages
Enzymes
Collagenase Type 1 : 250 u/ml
Medium
DMEM
Miscellaneous
Rat
Rat, SD, 6-8 month
Zhou, B. , Zhou, Y. and Tang, K.
The Effects of Substance P on Pluripotent Tendon Cells: an In Vitro and In Vivo Study. ,
J Musculoskelet Neuronal Interact
 14 , 349-58 , 2014
Cells
Pluripotent tendon
Enzymes
Medium
DMEM
Miscellaneous
Rat
Rat, male, 250-350g
Silver, N. , Proctor, GB, Arno, M. and Carpenter GH
Activation of mTOR Coincides with Autophagy During Ligation-induced Atrophy in the Rat Submandibular Gland. ,
Cell Death Dis
 1 , e14 , 2010
Cells
Submandibular acinar
Enzymes
Collagenase : 0.05%
Medium
PBS
Miscellaneous
Rat
Rat, SD, 8-10 week
Lin, A. , Hokugo, A. , Choi, J. and Nishimura, I.
Small Cytoskeleton-Associated Molecule, Fibroblast Growth Factor Receptor 1 Oncogene Partner 2/Wound Inducible Transcript-3.0 (FGFR1OP2/wit3.0), Facilitates Fibroblast-Driven Wound Closure. ,
Am J Pathol
 176 , 108-21 , 2010
Cells
Oral fibroblasts
Enzymes
Collagenase Type 1 : 200 u/ml
Medium
DMEM
Miscellaneous
Rat
Rat, Wistar, 7-9 week
Kaneko, N. , Rikimaru, T. , Fujimura, T. , Mori, S. , Hidaka, S. and Kaya, H.
Preparation of Rat Gingival Mitochondria with an Improved Isolation Method. ,
Int J Dent
 2010 , 275103 , 2010
Cells
Gingival mitochondria
Enzymes
Collagenase Type 1 : 0.115-0.130 %
Medium
HBSS
Miscellaneous
Rat
Rat, 8-10 week
Moghaddami, M. , Cleland, L. and Mayrhofer, G.
MHC II+ CD45+ Cells from Synovium-Rich Tissues of Normal Rats: Phenotype, Comparison with Macrophage and Dendritic Cell Lineages and Differentiation into Mature Dendritic Cells In Vitro. ,
Int Immunol
 17 , 1103 , 2005
Cells
Synovial cells
Enzymes
Collagenase Type 1 : 250 u/ml
Medium
RPMI 1640
Muscle
Rat
Rat, day 7
Vilmont, V. , Cadot, B. , Ouanoumou, G. and Gomes, E.
A System for Studying Mechanisms of Neuromuscular Junction Development and Maintence ,
Development
 143 , 2464 , 2016
Cells
Skeletal muscle
Enzymes
Collagenase : 0.05%
Medium
DMEM
Muscle
Rat
Rat, SD
Zeng, Y. , Liu, H. , Kang, K. , Wang, Z. , Hui, G. , Zhang, X. , Zhong, J. , Peng, W. , Ramchandran, R. , Raj, J. and Gou, D.
Hypoxia Inducible Factor-1 Mediates Expression of miR-322: Potential Role in Proliferation and Migration of Pulmonary Arterial Smooth Muscle Cells. ,
Sci Rep
 5 , 12098 , 2015
Cells
Pulmonary arterial smooth muscle
Enzymes
Elastase : 0.05%
Medium
Buffer
Muscle
Rat
Rat
Weisleder, N. , Zhou, J. and Ma, J.
Detection of Calcium Sparks in Intact and Permeabilized Skeletal Muscle Fibers. ,
Methods Mol Biol
 798 , 395 , 2012
Cells
Skeletal muscle fiber
Enzymes
Medium
Tyrode
Muscle
Rat
Rat
Weber, S. , Gratopp, A. , Akanbi, S. , Rheinlaender, C. , Sallmon, H. , Barikbin, P. and Koehne, P.
Isolation and Culture of Fibroblasts, Vascular Smooth Muscle, and Endothelial Cells From the Fetal Rat Ductus Arteriosus ,
Pediatr Res
 70 , 236 , 2011
Cells
Vascular smooth muscle
Enzymes
Medium
DMEM
Muscle
Rat
Rat
Pacak, C. and Cowan, D.
Fabrication of Myogenic Engineered Tissue Constructs. ,
J Vis Exp
 27 , e1137 , 2009
Cells
Myogenic
Enzymes
Medium
Ham's F-10
Muscle
Rat
Rat
Larkin, L. , Calve, S. , Kostrominova, T. and Arruda, E.
Structure and Functional Evaluation of Tendon-Skeletal Muscle Constructs Engineered In Vitro. ,
Tiss Eng
 12 , 3149-58 , 2006
Cells
myogenic
Enzymes
Neutral Protease : 8 u/ml
Collagenase Type 4 : 200 u/ml
Medium
DMEM
Muscle
Rat
Rat, Lewis, neonatal
Kim, J. , Hadlock, T. , Cheney, M. , Varvares, M. and Marler, J.
Muscle Tissue Engineering for Partial Glossectomy Defects. ,
Arch Facial Plast Surg
 5 , 403 , 2003
Cells
Myoblasts
Enzymes
Medium
Ham's F-10
Muscle
Rat
Rat, SD, 250 g
Jaggar JH.
Intravascular pressure regulates local and global Ca(2+) signaling in cerebral artery smooth muscle cells ,
Am J Physiol Cell Physiol
 281 , C439-48 , 2001
Cells
Arterial smooth muscle
Enzymes
Papain : 0.03%
Collagenase : 0.1%
Medium
see reference
Muscle
Rat
Rat, SD, 3 month
Stevenson S. , Rollence M. , Marshall-Neff J. and Liau G.
A Genetically Modified Adenoviral Vector Exhibits Enhanced Gene Transfer of Human Smooth Muscle Cells ,
J Vasc Res
 38(5) , 471 , 2001
Cells
Smooth muscle cells
Enzymes
Medium
M-199
Muscle
Rat
Rat, SD, male, 250-350 g
Hrometz, S. , Edelmann, S. , McCune, D. , Olges, J. , Hadley, R. , Perez ,D. and Piascik, M.
Expression of Multiple Alpha1-Adrenoceptors on Vascular Smooth Muscle: Correlation with the Regulation of Contraction ,
J Pharmacol Exp Ther
 290(1) , 452 , 1999
Cells
Vascular smooth muscle
Enzymes
Elastase : 0.0125%
Medium
DMEM
Muscle
Rat
Rat, Wistar, female, 10 weeks
Boulanger-Saunier, C. , Kattenburg, D. and Stoclet, J.
Cyclic AMP-dependent Phosphorylation of a 16kDa Protein in a Plasma Membrane-enriched Fraction of Rat Aortic Myocytes ,
F.E.B.S. Lett.
 193 , 283 , 1985
Cells
Myocytes
Enzymes
Collagenase : 0.1%
Medium
HBSS
Muscle
Rat
Rat, SD, 225-250g
Gunther S, Alexander RW, Atkinson WJ. and Gimbrone MA Jr.
Functional angiotensin II receptors in cultured vascular smooth muscle cells ,
J Cell Biol
 92 , 289 , 1982
Cells
Mesenteric artery smooth muscle cells
Enzymes
Medium
HBSS
Neural
Rat
Rat, 2-3 week
Lin, Y. and Chen, J.
Dorsal Root Ganglia Isolation and Primary Culture to Study Neurotransmitter Release. ,
J Vis Exp
 , 2018
Cells
Dorsal root ganglia
Enzymes
Medium
HBSS
Neural
Rat
Rat, SD, 3 month
Andersen, N. and Monje, P.
Isolation, Culture, and Cryopreservation of Adult Rodent Schwann Cells Derived from Immediately Dissociated Teased Fibers. ,
Methods Mol Biol
 1739 , 49-66 , 2018
Cells
Schwann
Enzymes
Medium
DMEM
Neural
Rat
Rat, SD, day 2
Maurel, P.
Preparation of Neonatal Rat Schwann Cells and Embryonic Dorsal Root Ganglia Neurons for In Vitro Myelination Studies. ,
Methods Mol Biol
 1739 , 17-37 , 2018
Cells
Schwann
Enzymes
Trypsin : 0.25%
Medium
DMEM
Neural
Rat
Rat, embryonic 14.5 day
Mirsky, R. and Jessen, K.
Isolation of Schwann Cell Precursors from Rodents. ,
Methods Mol Biol
 1739 , 3-15 , 2018
Cells
Schwann cell precursors
Enzymes
Medium
DMEM/ Ham's F-12
Neural
Rat
Rat, Wistar, 3 day
Fangmann, L. , Teller, S. , Stupakov, P. , Friess, H. , Ceyhan, G. and Demir, I.
3D Cancer Migration Assay with Schwann Cells. ,
Methods Mol Biol
 1739 , 317-325 , 2018
Cells
Dorsal root ganglia neurons
Enzymes
Medium
MEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.