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Tissue References Search Tool

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Bone
Bovine
Bovine
Buschmann, M. , Gluzband, Y. , Grodzinsky, A. and Hunziker, E.
Mechanical Compression Modulates Matrix Biosynthesis in Chondrocyte/Agarose Culture ,
J Cell Sci
 108 , 1497 , 1995
Cells
Chondrocytes
Enzymes
Medium
DMEM
Bone
Human

Human

Klauzen, P. , Semenova, D. , Kostina, D. , Uspenskiy, V. and Malashicheva, A.
Purinergic Signaling in Pathologic Osteogenic Differentiation of Aortic Valve Interstitial Cells from Patients with Aortic Valve Calcification. , Biomedicines Vol. 11 , 2023
Cells
Valve interstitial and endothelial
HUVEC
Osteoblast
Enzymes
Medium
ECM
Bone
Human
Human
Agarwal, S. , Loder, S. , Sorkin, M. , Li, S. , Shrestha, S. , Zhao, B. , Mishina, Y. , James, A. and Levi, B.
Analysis of Bone-Cartilage-Stromal Progenitor Populations in Trauma Induced and Genetic Models of Heterotopic Ossification. ,
Stem Cells
 34 , 1692-701 , 2016
Cells
Bone-cartilage-stromal
Enzymes
Medium
HBSS
Bone
Human
Human
Kode, A.,Manavalan1, J.,Mosialou1, I. , Bhagat G. and Rathinam, C, Luo, N et al.
Leukemogenesis Induced by an Activating β-catenin Mutation in Osteoblasts ,
Nature
 506 , 240 , 2014
Cells
Osteoblasts
Enzymes
Medium
MEM
Bone
Human
Human
Taylor, S. , Shah, M. and Orriss, I.
Generation of Rodent and Human Osteoblasts. ,
Bonekey Rep
 3 , 585 , 2014
Cells
Osteoblasts
Enzymes
Trypsin : 0.25%-1.0%
Medium
DMEM
Bone
Human
Human, 60+ year
Chen X. , Qian H. , Neff L. , Satomura K. and Horowitz M.
Thy-1 antigen Expression by Cells in the Osteoblast Lineage ,
J Bone Miner Res
 14 , 362 , 1999
Cells
Bone Cells, Osteoblasts
Enzymes
Collagenase Type 2 : 200-250 u/ml
Medium
DMEM
Bone
Human
Human
Fedarko, N.S. , Termine, J.D. , Young, M.F. and Robey, P.G.
Temporal Regulation of Hyaluronan and Proteoglycan Metabolism by Human Bone Cells in Vitro ,
J Biol Chem
 265 , 12200 , 1990
Cells
Osteoblasts
Enzymes
Collagenase Type 4 : 250 u/ml
Medium
DMEM
Bone
Mouse
Mouse
Mohrin, D. , Koukos, G. , Alabdulaaly, L. , Paw, J. , Hake, K. , Kolumam, G. , Bouxsein, M. , Baron, R. , Kutskova, Y. , Freund, A. , Liu, J. , Zavala-Solorio, J. , Bhargava, S. , Maxwell, Trumble, J. , Brito, A. , Hu, D. and Brooks, D.
Inhibition of Longevity Regulator PAPP-A Modulates Tissue Homeostasis via Restraint of Mesenchymal Stromal Cells. , Aging Cell Vol. 20 , e13313 , 2021
Cells
Bone and bone marrow stromal
Enzymes
Medium
PBS
Bone
Mouse
Mouse
Baryawno, N. , Przybylski, D. , Kowalczyk, M. , Kfoury, Y. , Severe, N. , Gustafsson, K. , Kokkaliaris, K. , Mercier, F. , Tabaka, M. , Hofree, M. , Dionne, D. , Papazian, A. , Lee, D. , Ashenberg, O. and Subramanian, A.
A Cellular Taxonomy of the Bone Marrow Stroma in Homeostasis and Leukemia. ,
Cell
 177 , 1915-1932.e16 , 2019
Cells
Bone and bone marrow stroma
Enzymes
Medium
Media 199
Bone
Mouse
Mouse
Wang, W. , Majihail, G. , Lui, C. and Zhou, L.
Osteoblast Sorting and Intracellular Staining of CXCL12. ,
Bio Protoc
 8 , 2018
Cells
Osteoblast
Enzymes
Medium
HBSS
Bone
Mouse
Mouse
Agarwal, S. , Loder, S. , Sorkin, M. , Li, S. , Shrestha, S. , Zhao, B. , Mishina, Y. , James, A. and Levi, B.
Analysis of Bone-Cartilage-Stromal Progenitor Populations in Trauma Induced and Genetic Models of Heterotopic Ossification. ,
Stem Cells
 34 , 1692-701 , 2016
Cells
Bone-cartilage-stromal
Enzymes
Medium
HBSS
Bone
Mouse
Mouse
Igarashi, H. , Akahoshi, N. , Ohto-Nakanishi, T. , Yasuda, D. and Ishii, S.
The Lysophosphatidic Acid Receptor LPA4 Regulates Hematopoiesis-Supporting Activity of Bone Marrow Stromal Cells. ,
Sci Rep
 5 , 11410 , 2015
Cells
Bone stromal
Enzymes
Medium
DMEM
Bone
Mouse
Mouse
Kode, A.,Manavalan1, J.,Mosialou1, I. , Bhagat G. and Rathinam, C, Luo, N et al.
Leukemogenesis Induced by an Activating β-catenin Mutation in Osteoblasts ,
Nature
 506 , 240 , 2014
Cells
Osteoblasts
Enzymes
Medium
MEM
Bone
Mouse
Mouse
Lee, D. , Choung, H. , Kim, H. , Gronostajski, R. , Yang, Y. , Ryoo, H. , Lee, Z. , Kim, H. , Cho, E. and Park, J.
NFI-C Regulates Osteoblast Differentiation Via Control of Osterix Expression. ,
Stem Cells
 32 , 2467-79 , 2014
Cells
Osteoblasts
Enzymes
Medium
MEM
Bone
Mouse
Mouse, 2-5 day
Taylor, S. , Shah, M. and Orriss, I.
Generation of Rodent and Human Osteoblasts. ,
Bonekey Rep
 3 , 585 , 2014
Cells
Osteoblasts
Enzymes
Trypsin : 0.25%-1.0%
Medium
DMEM
Bone
Mouse
Mouse, 4-22 month
Stern, A. , Stern, M. , Van Dyke, M. , Jahn, K. , Prideaux, M. and Bonewald, L.
Isolation and Culture of Primary Osteocytes from the Long Bones of Skeletally Mature and Aged Mice. ,
Biotechniques
 52 , 361 , 2012
Cells
Osteocytes
Enzymes
Collagenase Type 1 : 300 u/ml
Medium
MEM
Bone
Mouse
Mouse
Nakamura, Y. , Arai, F. , Iwasaki, H. , Hosokawa, K. , Kobayashi, I. , Gomei, Y. , Matsumoto, Y. , Yoshihara, H. and Suda, T.
Isolation and Characterization of Endosteal Niche Cell Populations that Regulate Hematopoietic Stem Cells. ,
Blood
 116 , 1422 , 2010
Cells
Endosteal cells
Enzymes
Medium
DMEM
Bone
Mouse
Mouse
Morikawa, S. , Mabuchi, Y. , Kubota, Y. , Nagai, Y. , Niibe, K. , Hiratsu, E. , Suzuki, S. , Miyauchi-Hara, C. , Nagoshi, N. , Sunabori, T. , Shimmura, S. , Miyawaki, A. , Nakagawa, T. , Suda, T. , Okano, H. and Matsuzaki, Y.
Prospective Identification, Isolation, and Systemic Transplantation of Multipotent Mesenchymal Stem Cells in Murine Bone Marrow. ,
J Exp Med
 206 , 2483-96 , 2009
Cells
Bone marrow
Enzymes
Collagenase : 0.2%
Medium
DMEM
Bone
Mouse
Mouse, male, 6-8 week old
Sakai, E. , Miyamoto, H. , Okamoto, K. , Kato, Y. , Yamamoto, K. and Sakai, H.
Characterization of Phagosomal Subpopulations Along Endocytic Routes in Osteoclasts and Macrophages ,
J. Biochem.
 130 , 823 , 2001
Cells
Osteoclasts
Enzymes
Medium
DMEM
Bone
Mouse
Mouse, 3-5 day, 6-8 week
Chen X. , Qian H. , Neff L. , Satomura K. and Horowitz M.
Thy-1 antigen Expression by Cells in the Osteoblast Lineage ,
J Bone Miner Res
 14 , 362 , 1999
Cells
Bone Cells, Osteoblasts
Enzymes
Collagenase Type 2 : 200-250 u/ml
Medium
DMEM
Bone
Mouse
Mouse, Swiss-Webster
Chen,T. and Feldman,D.
Regulation of 1,25-Dihydroxyvitamin D3 Receptors in Cultured Mouse Bone Cells ,
J Biol Chem
 256 , 5561 , 1981
Cells
Neonatal bone
Enzymes
Medium
Tris-buffered saline
Bone
Rat
Rat, 2 day
Jeon, J. , Lee, M. and Yang, H.
Differentiated Osteoblasts Derived Decellularized Extracellular Matrix to Promote Osteogenic Differentiation. ,
Biomater Res
 22 , 4 , 2018
Cells
Osteoblasts
Enzymes
Trypsin : 0.25%
Medium
DMEM
Bone
Rat
Rat, Wistar, 250g
Jacobs, F. , Gijsen, H. , Van de Vyver, M. and Ferris, W.
Vanadate Impedes Adipogenesis in Mesenchymal Stem Cells Derived from Different Depots within Bone. ,
Front Endocrinol
 7 , 108 , 2016
Cells
Proximal femur stem
Enzymes
Medium
HBSS
Bone
Rat
Rat, 2-3 day
Taylor, S. , Shah, M. and Orriss, I.
Generation of Rodent and Human Osteoblasts. ,
Bonekey Rep
 3 , 585 , 2014
Cells
Osteoblasts
Enzymes
Trypsin : 0.25%-1.0%
Medium
DMEM
Bone
Rat
Rat, fetal, 21 days of gestation
Owen, T. , Aronow, M. , Shalhoub, V. , Barone, L. , Wilming, L. , Tassinari, M. , Kennedy, M. , Pockwinse, S. , Lian, J. and Stein, G.
Progressive Development of the Rat Oseoblast Phenotype In Vitro: Reciprocal Relationships in Expression of Genes Associated with Osteoblast Proliferation and Differentiation During Formation of the Bone Extracellular Matrix ,
J Cell Physiol
 143 , 420 , 1990
Cells
Calvaria
Enzymes
Collagenase : 0.2%
Medium
MEM
Bone
Rat
Rat, newborn
Ernst, M. and Froesch, E.
Osteoblastlike Cells in a Serum-Free Methylcellulose Medium Form Colonies: Effects of Insulin and Insulinlike Growth Factor I ,
Calcif Tissue Int
 40 , 27 , 1987
Cells
Osteoblastlike cells
Enzymes
Medium
MEM
Bone
Rat
Rat, fetus, 17-21 day
Peck, W. , Birge, S. and Fedak, S.
Bone Cells: Biochemical and Biological Studies After Enzymatic Isolation ,
Science
 146 , 1476 , 1964
Cells
Calvaria
Enzymes
Collagenase : 0.01%-0.6%
Medium
Tris-buffered saline
Muscle
Bovine
Bovine
Lee, E. , Choi, J. , Hyun, J. , Cho, K. , Hwang, I. , Lee, H. , Chang, J. and Choi, I.
Steroid Effects on Cell Proliferation, Differentiation and Steroid Receptor Gene Expression in Adult Bovine Satellite Cells. ,
AJAS
 20 , 501 , 2007
Cells
Muscle satellite cells
Enzymes
Collagenase Type 2 : 300 u/ml
Medium
Krebs-Ringer Bicarbonate
Muscle
Bovine
Bovine
Yu, M. , McAndrew, R. , Al-Saghir, R. , Maier, K. , Medhora, M. , Roman, R. and Jacobs, E.
Nitric Oxide Contributes to 20-HETE-Induced Relaxation of Pulmonary Arteries. ,
J Appl Physiol
 93 , 1391 , 2002
Cells
Pulmonary artery endothelial and smooth muscle cells
Enzymes
Collagenase : 0.04-0.05%
Medium
RPMI-1640
Muscle
Canine
Canine
Parker, M. , Loretz, C. , Tyler, A. , Snider, L. , Storb. R. and Tapscott, S.
Inhibition of CD26/DPP-IV Enhances Donor Muscle Cell Engraftment and Stimulates Sustained Donor Cell Proliferation. ,
Skelet Muscle
 2 , 4 , 2012
Cells
Skeletal muscle
Enzymes
Collagenase Type 4 : 200 u/ml
Neutral Protease : 1 u/ml
Medium
DMEM
Muscle
Chicken
Chicken, 1-2 day
Dirksen W. , Vladic F. and Fisher S.
A Myosin Phosphatase Targeting Subunit Isoform Transition Defines a Smooth Muscle Developmental Phenotypic Switch ,
Am J Physiol/Cell
 278(3) , C589 , 2000
Cells
Gizzard and aorta smooth muscle
Enzymes
Medium
HBSS
Muscle
Chicken
Chick embryo
Hilfer, S. and Brown, J.
Collagenase. Its Effectiveness as a Dispersing Agent for Embryonic Chick Thyroid and Heart ,
Exp Cell Res
 65 , 246 , 1971
Cells
Thyroid
Muscle
Heart
Enzymes
Collagenase : 0.25%
Medium
Tyrode's saline, potassium free
Muscle
Feline
Cat, mongrel, adult, 2-4 kg
Follmer, C.H. , Ten Eick, R.E. and Yeh, J.Z.
Sodium Current Kinetics in Cat Atrial Myocytes ,
J Physiol
 384 , 169 , 1987
Cells
Myocytes
Enzymes
Collagenase : 0.12%
Medium
Kreb's Henseleit, CF
Muscle
Fish
Zebrafish
Alexander, M. , Kawahara, G. , Kho, A. , Howell, M. , Pusack, T. , Myers, J. , Montanaro, F. , Zon, L. , Guyon, J. and Kunkel, L.
Isolation and Transcriptome Analysis of Adult Zebrafish Cells Enriched for Skeletal Muscle Progenitors. ,
Muscle Nerve
 43 , 741-50 , 2011
Cells
Myogenic progenitor
Enzymes
Medium
PBS
Muscle
Frog
Frog, Xenopus laevis, embryos stage 17 & 19
Stollberg, J. and Fraser, S.
Acetylcholine Receptors and Con A-Binding Sites on Xenopus Muscle Cells ,
J Cell Biol
 107 , 1397 , 1988
Cells
Muscle
Enzymes
Collagenase : 0.10%
Medium
Steinberg's solution
Muscle
Guinea-Pig
Guinea-pig
Shieh CC, Feng J, Buckner SA, Brioni JD, Coghlan MJ, Sullivan JP, Gopalakrishnan M.
Functional implication of spare ATP-sensitive K(+) channels in bladder smooth muscle cells ,
J Pharmacol Exp Ther
 296 , 669-75 , 2001
Cells
Bladder smooth muscle
Enzymes
Collagenase Type 2 : 0.1-0.2%
Medium
Krebs-Ringer bicarbonate

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.