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Tissue References Search Tool

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Brain
Bovine
Bovine
Wolburg H, Neuhaus J, Kniesel U, Krauss B, Schmid EM, Ocalan M, Farrell C, Risau W.
Modulation of tight junction structure in blood-brain barrier endothelial cells. Effects of tissue culture, second messengers and cocultured astrocytes ,
J Cell Sci
 107 , 1347 , 1994
Cells
Brain endothelial cells
Enzymes
Medium
DMEM
Brain
Bovine
Bovine, adult
Machi, T. , Kassell, N.F. and Scheld, W.M.
Isolation and Characterization of Endothelial Cells From Bovine Cerebral Arteries ,
In Vitro Cell Dev Biol
 26 , 291 , 1990
Cells
Cerebral artery
Endothelial
Enzymes
Collagenase : 0.2%
Medium
HBSS
Brain
Bovine
Bovine
Estrada, C. , Bready, J. , Berliner, J. and Cancilla, P.
Choline Uptake by Cerebral Capillary Endothelial Cells in Culture ,
J Neurochem
 54 , 1467 , 1990
Cells
Capillary endothelial
Enzymes
Collagenase : 0.1%
Medium
DMEM
Brain
Bovine
Bovine
Goetz, I.. , Warren, J. , Estrada, C. , Roberts, E. and Krause, D.
Long-Term Cultivation of Arterial and Capillary Endothelium From Adult Bovine Brain ,
In Vitro Cell Dev Biol
 21 , 172 , 1985
Cells
Endothelial
Brain arteries
Enzymes
Medium
Dulbecco's PBS
Brain
Human
Human
Mizee, M. , Miedema, S. , van der Poel, M. , Adelia , S. , van Strien, M. , Melief, J. , Smolders, J. , Hendrickx, D. , Heutinck, K. , Hamann, J. and Huitinga, I.
Isolation of Primary Microglia from the Human Post-Mortem Brain: Effects of Ante- and Post-Mortem Variables. ,
Acta Neuropathol
 5 , 16 , 2017
Cells
Microglia
Enzymes
Collagenase Type 1 : 300 u/ml
Trypsin : 0.125%
Medium
DMEM
Brain
Human
Human
Scherpelz, K. , Lu, J.,Tycko, R. and Meredith, S.
Preparation of Amyloid Fibrils Seeded from Brain and Meninges ,
Methods Mol Biol
 1345 , 299 , 2016
Cells
Amyloid Fibrils
Enzymes
Medium
50mM Tris
Brain
Human
Human, 25-81 yr
Volovitz, I, Shapira, N. , Ezer, H. , Gafni, A. , Lustgarten, M. , Alter, T. , Ben-Horin, I. , Barzilai, O. , Shahar, T. , Kanner, A. , Fried, I. , Veshchev, I. , Grossman, R. and Ram, Z,
A Non-Aggressive, Highly Efficient, Enzymatic Method for Dissociation of Human Brain-Tumors and Brain-Tissues to Viable Single-Cells. ,
BMC Neurosci
 17 , 30 , 2016
Cells
Brain tumor
Enzymes
Neutral Protease : 0.11 u/ml
Hyaluronidase : 1,000 u/ml
Medium
HBSS
Brain
Human
Hussein, D. , Punjaruk, W. , Storer, L. , Shaw, L. , Othman, R. , Ottoman, R. , Peet, A. , Miller, S. , Bandopadhyay, G. , Heath, R. , Kumari, R. , Bowman, K. , Braker, P. , Rahman, R. , Jones, G. , Watson, S. and Lowe, J.
Pediatric Brain Tumor Cancer Stem Cells: Cell Cycle Dynamics, DNA Repair, and Etoposide Extrusion. ,
Neuro Oncol
 13 , 70-83 , 2011
Cells
Brain tumor
Enzymes
Medium
DMEM/F12
Brain
Human
Human
Sauvageot, C. , Weatherbee, J. , Kesari, S. , Winters, S. , Barnes, J. , Dellagatta, J. , Ramakrishna, N. , Stiles, C. , Kung, A. , Kieran, M. and Wen, P.
Efficacy of the HSP90 Inhibitor 17-AAG in Human Glioma Cell Lines and Tumorigenic Glioma Stem Cells. , Neuro Oncol Vol. 11 , 109 , 2009
Cells
Tumor
Enzymes
Medium
see reference
Brain
Human
Human
Gerhart, D. Z. , Broderius, M. A. and Drewes, L. R.
Cutlured Human and Canine Endothelial Cells from Brain Microvessels ,
Brain Res Bull
 21 , 785 , 1988
Cells
Microvessels
Enzymes
Medium
DMEM
Brain
Human
Human, 15-54 years
Vinters, H.V. , Reave, S. , Costello, P. , Girvin, J.P. and Moore, S.A.
Isolation and Culture of Cells Derived From Human Cerebral Microvessels ,
Cell Tissue Res
 249 , 657 , 1987
Cells
Microvessels
Enzymes
Collagenase : 0.1%
Medium
Serum-free modified Lewis medium
Brain
Monkey
Monkey, rhesus
Marcondes MC, Burudi EM, Huitron-Resendiz S, Sanchez-Alavez M, Watry D, Zandonatti M, Henriksen SJ, Fox HS.
Highly activated CD8(+) T cells in the brain correlate with early central nervous system dysfunction in simian immunodeficiency virus infection ,
J Immunol
 167 , 5429-38 , 2001
Cells
Brain cells
Enzymes
Medium
HBSS
Brain
Mouse
Adult and neonatal
Calvo, Belen, Rubio, F. , Fernandez, M. and Tranque, P.
Dissociation of Neonatal and Adult Mice Brain for Simultaneous Analysis of Microglia, Astrocytes and Infiltrating Lymphocytes by Flow Cytometry. , IBRO Rep Vol. 8 , 36-47 , 2020
Cells
Microglia, astrocytes, lymphocytes
Enzymes
Collagenase Type 2 : 120 u/ml
Papain : 20 u/ml
Neutral Protease : 1.2 u/ml
Medium
EBSS
Brain
Mouse
Mouse, adult
Swartzlander, D. , Propson, N. , Roy, E. , Saito, T. , Saido, T. , Wang, B. and Zheng, H.
Concurrent Cell Type-Specific Isolation and Profiling of Mouse Brains in Inflammation and Alzheimer's Disease. ,
JCI Insight
 3 , 2018
Cells
Astrocytes, microglia, endothelia, oligodendrocytes
Enzymes
Papain : 20 u/ml
Medium
HBSS
Brain
Mouse
Mouse
Baruch, K. , Rosenzweig, N. , Kertser, A. , Deczkowska, A. , Sharif, A. , Spinrad, A. , Tsitsou-Kampeli, A. , Sarel, A. , Cahalon, L. and Schwartz, M.
Breaking Immune Tolerance by Targeting Foxp3(+) Regulatory T Cells Mitigates Alzheimer's Disease Pathology. ,
Nat Commun
 6 , 7967 , 2015
Cells
Chloroid plexus
Enzymes
Collagenase Type 4 : 400 u/ml
Medium
PBS
Brain
Mouse
Mouse
Shah. G. , Price, T. , Banks, W. , Morofuji, Y. , Kovac, A. , Ercal, N. , Sorenson, C. , Shin, E. and Sheibani, N.
Pharmacological Inhibition of Mitochondrial Carbonic Anhydrases Protects Mouse Cerebral Pericytes from High Glucose-Induced Oxidative Stress and Apoptosis. ,
J Pharmacol Exp Ther
 344 , 637-45 , 2013
Cells
Cerebral pericytes
Enzymes
Medium
DMEM
Brain
Mouse
Mouse
Ryg-Cornejo, V. , Ioannidis, L. and Hansen, D.
Isolation and Analysis of Brain-Sequestered Leukocytes from Plasmodium Berghei ANKA-Infected Mice. ,
J Vis Exp
 71 , e50112 , 2013
Cells
Brain-sequestered leukocytes
Enzymes
Medium
RPMI
Brain
Mouse
Mouse, 6-12 week
Chung, W. , Farley, J. , Swenson, A. , Barnard, J. , Hamilton, G. , Chiposi, R. and Drummond, H.
Extracellular Acidosis Activates ASIC-like Channels in Freshly Isolated Cerebral Artery Smooth Muscle Cells. ,
Am J Physiol Cell Physiol
 298 , C1198 , 2010
Cells
Vascular smooth muscle cells
Enzymes
Papain : 0.05%
Elastase : 0.05%
Medium
PBS
Brain
Mouse
Mouse, SD, 8-12 week
O'Donnell SL, Frederick TJ, Krady JK, Vannucci SJ, Wood TL.
IGF-I and microglia/macrophage proliferation in the ischemic mouse brain ,
Glia
 39 , 85 , 2002
Cells
Microglia
Enzymes
Medium
RPMI-1640
Brain
Porcine
Mini pigs, Yucatan (Susscrofa Yucatan), 4-6 months
Robinson, D.H. , Kang,Y. , Deschner, S.H. and Nielsen, T.B.
Morphologic Plasticity and Periodicity : Porcine Cerebral Microvascular Cells in Culture ,
In Vitro Cell Dev Biol
 26 , 169 , 1990
Cells
Microvascular
Enzymes
Collagenase : 0.1%
Medium
HBSS
Brain
Rat
Rat, neonatal
Peptan, I. , Hong, L. and Evans, C.
Multiple Differentiation Potentials of Neonatal Dura Mater-Derived Cells. ,
Neurosurgery
 60 , 346 , 2007
Cells
Dura mater
Enzymes
Medium
DMEM
Brain
Rat
Rat, Wistar, male
Floris S, Van den Born J, van der Pol SM, Dijkstra CD, De Vries HE.
Heparan sulfate proteoglycans modulate monocyte migration across cerebral endothelium ,
J Neuropathol Exp Neurol
 62 , 780 , 2003
Cells
Cerebral endothelial
Enzymes
Medium
MEM
Brain
Rat
Rat, wistar, 250-300 g
Brzezinska AK, Gebremedhin D, Chilian WM, Kalyanaraman B, Elliott SJ.
Peroxynitrite reversibly inhibits Ca(2+)-activated K(+) channels in rat cerebral artery smooth muscle cells ,
Am J Physiol Heart Circ Physiol
 278 , H1883 , 2000
Cells
Cerebral artery smooth muscle cells
Enzymes
Papain : 1.5 mg/ml
Collagenase Type 4 : 1.5 mg/ml
Medium
Physiological Salt Solution
Brain
Rat
Rat, SD, adult
Doron, D. , Jacobowitz, D. , Heldman, E. , Feurerstein, G. , Pollard, H. and Hallenbeck, J.
Extracellular Matrix Permits the Expression of Von Willebrand's Factor, Uptake of Di-I-Acetylated Low Density Lipoprotein and Secretion of Prostacyclin in Cultures of Endothelial Cells from Rat Brain Microvessels ,
In Vitro Cell Dev Biol
 27A , 689 , 1991
Cells
Endothelial
Enzymes
Medium
Medium 199
Brain
Rat
Rat, SD, female
Matsuda, M.
Serum Proteins Enhance Aggregate Formation of Dissociated Fetal Rat Brain Cells in an Aggregating Culture ,
In Vitro Cell Dev Biol
 24 (10) , 1031 , 1988
Cells
Fetal rat brain
Enzymes
Medium
DMEM
Brain
Rat
Rat, Wistar-Kyoto, male, 100 - 200 g
Diglio, C.A. , Grammas, P. , Filiberto Giacomelli, M.S. and Wiener, J.
Primary Culture of Rat Cerebral Microvascular Endothelial Cells ,
Lab Invest
 46 , 554 , 1982
Cells
Endothelial
Cerebral
Enzymes
Medium
HBSS
Brain
Rat
Rat, Wistar, male, 300-500 g
Williams, S. , Gillis, J. , Matthews, M. , Wagner, R. and Bitensky, M.
Isolation and Characterization of Brain Endothelial Cells: Morphology and Enzyme Activity ,
J Neurochem
 35 (2) , 374 , 1980
Cells
Microvessels
Endothelial
Enzymes
Medium
Ringers-HEPES buffer
Parotid
Mouse
Mouse
Ogawa, M. , Oshima, M. , Imamura, A. , Sekine, Y. , Ishida, K. , Yamashita, K. , Nakajima, K. , Hirayama, M. , Tachikawa, T. and Tsuji, T.
Functional Salivary Gland Regeneration by Transplantation of a Bioengineered Organ Germ. ,
Nat Commun
 4 , 2498 , 2013
Cells
Parotid and sublingual glandular
Enzymes
Neutral Protease : 50 u/ml
Collagenase Type 1 : 100 u/ml
Medium
PBS
Parotid
Rat
Rat, Wistar, male, 130 g
Looms, D. , Dissing, S. , Tritsaris, K. , Pedersen, A. and Nauntofte, B.
Adrenoceptor-Activated Nitric Oxide Synthesis in Salivary Acinar Cells. ,
Adv Dent Res
 14 , 62 , 2000
Cells
Acinar
Enzymes
Collagenase : 75 u/ml
Hyaluronidase : 153 u/ml
Medium
RPMI 1640
Parotid
Rat
Rat, SD, 100-120 g
D'Silva NJ, DiJulio DH, Belton CM, Jacobson KL, Watson EL.
Immunolocalization of Rap1 in the rat parotid gland: detection on secretory granule membranes ,
J Histochem Cytochem
 45 , 965-73 , 1997
Cells
Parotid acinar cells
Enzymes
Collagenase Type 2 : 90 u/ml
Medium
Krebs-Henseleit Bicarbonate
Parotid
Rat
Rat, SD, male
Prasad, K.N. , Edwards-Prasad, J. , Carvaiho, I. , LaRosa, F.G. , Baibinder, E. , Meyers, A. and Quissell, D.
Establishment of Primary Cultures of Rat and Human Parotid Epithelial Cells for Transfection Experiments ,
In Vitro Cell Dev Biol
 28 , 493 , 1992
Cells
Epithelial
Enzymes
Collagenase : 50-75 u/ml
Hyaluronidase : 0.1%
Medium
HBSS CF
Parotid
Rat
Rat, SD, albino, male, 150-200 g
Mangos, J. , McSherry, N. , Butcher, F. , Irwin, K. and Barber, T.
Dispersed Rat Parotid Acinar Cells (Morphological, Functional Character) ,
Am J Physiol
 229 (3) , 560 , 1975
Cells
Acinar, parotid
Enzymes
Trypsin : 0.01%
Collagenase : 40-50 u/ml
Hyaluronidase : 0.10%
Medium
HBSS CMF

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.