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Tissue References Search Tool

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Endothelial
Human
Human
Grant, D.S. , Lelkes, P.I. , Fukuda, K. and Kleinman, H.K.
Intracellular Mechanisms Involved in Basement Membrane Induced Blood Vessel Differentiation In Vitro ,
In Vitro Cell Dev Biol
 27 , 327 , 1991
Cells
Umbilical vein
HUVEC
Enzymes
Collagenase : 0.1%
Medium
Cord Buffer
(See Reference)
Endothelial
Human
Human
Whitehead, R. and Eeden, P.
A Method For the Prolonged Culture of Colonic Epithelial Cells ,
J Tiss Cul Meth
 13 , 103 , 1991
Cells
Crypt cells
Enzymes
Collagenase : 125 u/ml
Medium
RMPI 1640
Endothelial
Human
Human
Fischer, E. , Stingl, A. and Kirkpatrick, C.
Migration Assay for Endothelial Cells in Multiwells Application to Studies on the Effect of Opioids ,
J Immunol Methods
 128 , 235 , 1990
Cells
Endothelial
Enzymes
Medium
Medium 199
Endothelial
Human
Human
Muller WA, Ratti CM, McDonnell SL, Cohn ZA.
A human endothelial cell-restricted, externally disposed plasmalemmal protein enriched in intercellular junctions ,
J Exp Med
 170 , 399-414 , 1989
Cells
Human umbilical vein endothelial cells
Enzymes
Collagenase Type 2 : 75 u/ml
Medium
M199
Endothelial
Human
Human
Hoshi, H. , Kan, M. , Chen, J. and McKeehan, W.
Comparative Endocrinology-Paracrinology-Autocrinology of Human Adult Large Vessel Endothelial and Smooth Muscle Cells ,
In Vitro Cell Dev Biol
 24 (4) , 309 , 1988
Cells
Umbilical cord
Smooth muscle
Enzymes
Collagenase : 0.1%
Medium
HEPES
Endothelial
Human
Human
Gerhart, D. Z. , Broderius, M. A. and Drewes, L. R.
Cutlured Human and Canine Endothelial Cells from Brain Microvessels ,
Brain Res Bull
 21 , 785 , 1988
Cells
Microvessels
Enzymes
Medium
DMEM
Endothelial
Human
Human
Hoshi, H. and McKeehan, W.
Isolation, Growth Requirements, Cloning, Prostacylclin Production and Life-Span of Human Adult Endothelial Cells in Low Serum Culture Medium ,
In Vitro Cell Dev Biol
 22 (1) , 51 , 1986
Cells
Endothelial
Enzymes
Collagenase : 0.1%
Medium
HEPES
Endothelial
Human
Human
Sharefkin, J.B. , Fairchild, K.D. , Albus, R.A. , Cruess, D.F. and Rich, N.M.
The Cytoxic Effect of Surgical Glove Powder Particles on Adult Human Vascular Endothelial Cell Cultures: Implications for Clinical Uses of Tissue Culture Techniques ,
J Surg Res
 41 , 463 , 1986
Cells
Endothelial
Saphenous vein
Enzymes
Medium
PBS, CMF
Endothelial
Human
Human
Glassberg, M. , Bern, M. , Coughlin, S. , Haudenschild, C. , Hoyer, L. and Antoniades, H.
Cultured Endothelial Cells Derived from the Human Iliac Arteries ,
In Vitro
 18 , 859 , 1982
Cells
Iliac arteries
Enzymes
Collagenase : 0.25%
Medium
PBS w/Ca++, Mg++, & BSA
(see reference)
Endothelial
Human
Human, adult
Ashida, E. , Johnson, A. and Lipsky, P.
Human endothelial cell-lymphocyte interaction. Endothelial cells function as accessory cells necessary for mitogen-induced human T lymphocyte activation in vitro ,
J Clin Invest
 67 , 1490 , 1981
Cells
Peripheral blood mononuclear
Monocytes
T cells
Endothelial
Enzymes
Collagenase : 0.25%
Medium
RPMI 1640
Endothelial
Human
Human (adult and child)
Folkman, J. , Haudenschild, C. C. and Zetter, B. R.
Long-term Culture of Capillary Endothlial Cells ,
Proc Natl Acad Sci U S A
 76 , 5217 , 1979
Cells
Foreskin
Enzymes
Collagenase : 0.5%
Medium
Dulbecco's MEM w/10% calf serum
Endothelial
Human
Human
Gimbrone Jr.
Culture of Vascular Endothelium ,
Prog Hemost Thromb
 3 , 1 , 1976
Cells
Umbilical vein
Enzymes
Collagenase Type 1 : 125 u/ml
Medium
Dulbecco's PBS
Endothelial
Human
Human
Gimbrone, M.A. , Cotran, R.S. and Folkman, J.
Human Vascular Endothelial Cells in Culture: Growth and DNA Synthesis ,
J Cell Biol
 60 , 673 , 1974
Cells
Umbilical cord
Enzymes
Collagenase : 0.1%
Medium
Dulbecco's PBS
Endothelial
Human
Human
Jaffe, E. , Nachman, R. , Becker, C. and Minick, C.
Culture of Human Endothelial Cells Derived from Umbilical Veins. Identification by Morphologic and Immunologic Criteria ,
J Clin Invest
 52 , 2745 , 1973
Cells
Umbilical cord
Enzymes
Collagenase : 0.2%
Medium
Cord buffer
(See Reference)
Endothelial
Mouse
Mouse, 4 week
Esteban, S. , Clemente, C. , Koziol, A. , Gonzalo, P. , Rius, C. , Martinez, F. , Linares, P. , Chaparro, M. , Urzainqui, A. , Andres, V. , Seiki, M. , Gisbert, J. and Arroyo, A.
Endothelial MT1-MMP Targeting Limits Intussusceptive Angiogenesis and Colitis via TSP1/Nitric Oxide Axis. ,
EMBO Mol Med
 12 , e10862 , 2020
Cells
Aortic endothelial
Enzymes
Elastase : 0.25%
Medium
DMEM
Endothelial
Mouse
Mouse, 2 month
Sabbagh, M. and Nathans, J.
A Genome-wide View of the De-differentiation of Central Nervous System Endothelial Cells in Culture ,
Elife
 , 1 , 2020
Cells
Endothelial, CNS
Enzymes
PDS kit : with modifications
Stemxyme 1 : 0.1%
Medium
EC growth medium
Endothelial
Mouse
Mouse
Luo, S. , Truong, A. and Makino, A.
Isolation of Mouse Coronary Endothelial Cells. ,
J Vis Exp
 , 2016
Cells
Coronary endothelial
Enzymes
Medium
Kreb's
Endothelial
Mouse
Mouse
Wang, J. , Sun, C. , Gerdes, N. , Liu, C. , Liao, M. , Liu, J. , Shi, M. , He, A. , Zhou, Y. , Sukhova, G. , Chen, H. , Cheng, X. , Kuzuya, M. , Murohara, T. , Zhang, J. , Cheng, X. , Jiang, M. , Shull, G. and Rogers, S.
Interleukin 18 Function in Atherosclerosis is Mediated by the Interleukin 18 Receptor and the Na-Cl Co-Transporter. ,
Nat Med
 21 , 820-6 , 2015
Cells
Endothelial lung
Enzymes
Medium
DMEM
Endothelial
Mouse
Mouse
Xiao, L. , McCann, J. and Dudley, A.
Isolation and Culture Expansion of Tumor-specific Endothelial Cells. ,
J Vis Exp
 , e53072 , 2015
Cells
Endothelial
Enzymes
Medium
DMEM
Endothelial
Mouse
Mouse
Kochukov, M. , Balasubramanian, A. , Abramowitz, J. , Birnbaumer, L. and Marrelli, S.
Activation of Endothelial Transient Receptor Potential C3 Channel is Required for Small Conductance Calcium-Activated Potassium Channel Activation and Sustained Endothelial Hyperpolarization and Vasodilation of Cerebral Artery. ,
J Am Heart Assoc
 3 , 2014
Cells
Cerebral artery endothelial
Enzymes
Neutral Protease : 4 u/ml
Elastase : 1 u/ml
Collagenase Type 1 : 120 u/ml
Medium
DMEM
Endothelial
Mouse
Mouse
Kazerounian, S. , Gerald, D. , Huang, M. , Chin, R, Udayakumar, D, Zheng, N. , O'Donnell, R. , Perruzzi, C. , Mangiante, L. , Pourat, J. , Phung, T. , Bravo-Nuevo, A. , Shechter, S. , McNamara, S. and Duhadaway, K.
RhoB Differentially Controls Akt Function in Tumor Cells and Stromal Endothelial Cells During Breast Tumorigenesis. ,
Cancer Res
 73 , 50 , 2013
Cells
Tumor endothelial
Enzymes
Medium
HBSS
Endothelial
Mouse
Mouse, 8-14 week
Imoukhuede, P. and Popel, A.
Expression of VEGF Receptors on Endothelial Cells in Mouse Skeletal Muscle. ,
PLoS ONE
 7 , e44791 , 2012
Cells
Endothelial
Enzymes
Medium
HBSS
Endothelial
Mouse
Mouse, 7-10 week
Follenzi, A. , Benten, D. , Novikoff, P. , Faulkner, L. , Raut, S. and Gupta, S.
Transplanted Endothelial Cells Repopulate the Liver Endothelium and Correct the Phenotype of Hemophilia A Mice. ,
J Clin Invest
 118 , 935 , 2008
Cells
Liver endothelial
Enzymes
Collagenase : 0.03%
Medium
DMEM
Endothelial
Mouse
Mouse, 4 week
Kondo, S. , Scheef, E. , Sheibani, N. and Sorenson, C.
PECAM-1 Isoform-Specific Regulation of Kidney Endothelial Cell Migration and Capillary Morphogenesis. ,
Am J Physiol Cell Physiol
 292 , C2070 , 2007
Cells
Endothelial kidney
Enzymes
Medium
DMEM
Endothelial
Mouse
Mouse, embryonic or yolk sac
Braren Rickmer, Hu Huiqing, Kim YungHae, Beggs HilaryE, Reichardt LouisF, Wang Rong.
Endothelial FAK is essential for vascular network stability, cell survival, and lamellipodial formation ,
J Cell Biol
 172 , 151-62 , 2006
Cells
Endothelial
Enzymes
Medium
PBS
Endothelial
Mouse
Mouse, neonatal
Cha, S. , Talavera, D. , Demir, E. , Nath, A. and Sierra-Honigmann, M.
A Method of Isolation and Culture of Microvascular Endothelial Cells from Mouse Skin. ,
Microvasc Res
 70 , 198 , 2005
Cells
Microvascular endothelial
Enzymes
Medium
DMEM
Endothelial
Mouse
Mouse, 4 week
Su X, Sorenson CM. and Sheibani N.
Isolation and characterization of murine retinal endothelial cells ,
Mol Vis
 9 , 171 , 2003
Cells
Retinal endothelial cells
Enzymes
Medium
DMEM
Endothelial
Mouse
Mouse, male
Izawa D, Tanaka T, Saito K, Ogihara H, Usui T, Kawamoto S, Matsubara K, Okubo K. and Miyasaka M
Expression profile of active genes in mouse lymph node high endothelial cells ,
Int Immunol
 11(12) , 1989 , 1999
Cells
Endothelial cells from lymph node
Enzymes
Medium
PBS
Endothelial
Mouse
Mouse, 2-4 week
Sapatino, B. , Welsh, C. , Smith, C. , Bebo, B. and Linticum, D.
Cloned Mouse Cerebrovascular Endothelial Cells That Maintain Their Differentiation Markers for Factor VIII, Low Density Lipoprotein, and Angiotensin-Converting Enzyme ,
In Vitro Cell Dev Biol
 29A , 923 , 1993
Cells
Cerebrovascular Endothelial
Enzymes
Medium
PBS
Endothelial
Porcine
Porcine, 6 month
Balaoing, L. , Post, A. , Lin, A. , Tseng, H. , Moake, J. and Grande-Allen, K.
Laminin Peptide-Immobilized Hydrogels Modulate Valve Endothelial Cell Hemostatic Regulation. ,
PLoS ONE
 10 , e0130749 , 2015
Cells
Valvular endothelial
Enzymes
Medium
PBS
Endothelial
Porcine
Porcine
Gould, R. and Butcher, J.
Isolation of Valvular Endothelial Cells. ,
J Vis Exp
 46 , e2158 , 2010
Cells
Valvular endothelial
Enzymes
Collagenase Type 2 : 300-600 u/ml
Medium
DMEM
Endothelial
Porcine
Porcine
(also bovine)
Nugent, H. and Edelman, E.
Endothelial Implants Provide Long-Term Control of Vascular Repair in a Porcine Model of Arterial Injury ,
J Surg Res
 99 , 228 , 2001
Cells
Endothelial
Medium
DMEM w/ 10% calf serum
Endothelial
Porcine
Porcine
Kwak HJ, Lee SJ, Lee YH, Ryu CH, Koh KN, Choi HY. and Koh GY.
Angiopoietin-1 inhibits irradiation- and mannitol-induced apoptosis in endothelial cells ,
Circulation
 101(19) , 2317 , 2000
Cells
HUVEC, porcine pulmonary arterial endothelial cells
Enzymes
Medium
DMEM
Endothelial
Porcine
Porcine, 6-7 month
Hill-Kapturczak N, Kapturczak MH, Block ER, Patel JM, Malinski T, Madsen KM. and Tisher CC.
Angiotensin II-stimulated nitric oxide release from porcine pulmonary endothelium is mediated by angiotensin IV ,
J Am Soc Nephrol
 10(3) , 481 , 1999
Cells
Porcine pulmonary endothelial
Enzymes
Medium
RPMI 1640
Endothelial
Porcine
Porcine
Dickinson, E. and Slakey, L.
Plasma-derived Serum as a Selective Agent to Obtain Endothelial Cultures from Swine Aorta ,
In Vitro
 18 , 63 , 1982
Cells
Endothelial
Aortic
Enzymes
Medium
Dulbecco-Vogt MEM w/o serum
Endothelial
Porcine
Porcine, 30-40 kg
Merrilees, M.J. and Scott, L.
Interaction of Aortic Endothelial and Smooth Muscle Cells in Culture ,
Atherosclerosis
 39 , 147 , 1981
Cells
Endothelial
Thoracic aorta
Enzymes
Trypsin : 0.05%
Medium
Medium 199 and 0.01M EDTA

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.