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Tissue References Search Tool

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Stem
Mouse
Mouse
Rountree, C. , Ding, W. , Dang, H. , Vankirk, C. and Crooks, G.
Isolation of CD133+ Liver Stem Cells for Clonal Expansion. ,
J Vis Exp
 56 , e3183 , 2011
Cells
CD133+ stem
Enzymes
Pronase : 0.05%
Medium
DMEM/F12
Stem
Mouse
Mouse, postnatal
Sher, F. , Rossler, R. , Brouwer, N. , Balasubramaniyan, V. , Boddeke, E. and Copray, S.
Differentiation of Neural Stem Cells Into Oligodendrocytes: Involvement of the Polycomb Group Protein Ezh2. ,
Stem Cells
 26 , 2875 , 2008
Cells
Astrocytes
Enzymes
Trypsin : 0.25%
Deoxyribonuclease I : 1,000 u/ml
Medium
HBSS
Stem
Mouse
Mouse, 12 week
Taddei, I. , Deugnier, M. , Faraldo, M. , Petit, V. , Bouvard, D. , Medina, D. , Fassler, R. , Thiery, J. and Glukhova, M.
Beta1 Integrin Deletion from the Basal Compartment of the Mammary Epithelium Affects Stem Cells ,
Nat Cell Biol
 10 , 716-22 , 2008
Cells
Mammary epithelial
Enzymes
Collagenase : 0.3%
Hyaluronidase : 100 u/ml
Trypsin : 0.25%
Medium
see reference
Stem
Mouse
Mouse
Li Wen-Lin, Su Juan, Yao Yu-Cheng, Tao Xin-Rong, Yan Yong-Bi, Yu Hong-Yu, Wang Xin-Min, Li Jian-Xiu, Yang Yong-Ji, Lau Joseph T Y, Hu Yi-Ping.
Isolation and characterization of bipotent liver progenitor cells from adult mouse ,
Stem Cells
 24 , 322-32 , 2006
Cells
Liver epithelial progenitor cells
Enzymes
Medium
DMEM
Stem
Mouse
Mouse, day 7
Lee, A, Kessler, J, Read, T, Kaiser, C, Corbeil, D, Huttner ,W, Johnson, J, Wechsler-Reya, R.
Isolation of Neural Stem Cells from the Postnatal Cerebellum ,
Nat Neurosci
 8 , 723 , 2005
Cells
Cerebellar stem cells
Enzymes
Papain : 10u/ml
Deoxyribonuclease I : 250 u/ml
Medium
Dulbecco's PBS
Stem
Mouse
Mouse
Boulanger CA, Smith GH.
Reducing mammary cancer risk through premature stem cell senescence ,
Oncogene
 20 , 2264-72 , 2001
Cells
Mammary epithelial stem
Enzymes
Hyaluronidase : 0.1%
Pronase : 1.25%
Medium
DMEM
Stem
Mouse
Mouse, 4-8 month
Gritti A, Frolichsthal-Schoeller P, Galli R, Parati E, Cova L, Pagano S, Bjornson C. and Vescovi A.
Epidermal and Fibroblast Growth Factors Behave as Mitogenic Regulators for a Single Multipotent Stem Cell-like Population from the Subventricular Region of the Adult Mouse forebrain ,
J Neurosci
 19(9) , 3287 , 1999
Cells
Neural subventricular zone
Enzymes
Trypsin : 0.13%
Hyaluronidase : 0.067%
Medium
DMEM/F12
Stem
Ovine
Ovine, 3-5 year
Letouzey, V. , Tan, K. , Deane, J. , Ulrich, D. , Gurung, S. , Ong, Y. and Gargett, C.
Isolation and Characterisation of Mesenchymal Stem/Stromal Cells in the Ovine Endometrium. ,
PLoS ONE
 10 , e0127531 , 2015
Cells
Endometrial stromal
Enzymes
Medium
DMEM/F-12
Stem
Porcine
Porcine, 1-4 day
Park, M. , Park, J. , Kim, M. , Lee, K. , Park, H. , Yun, J. , Choi, J. , Lee, E. and Lee, S.
Development of a High-Yield Technique to Isolate Spermatogonial Stem Cells From Porcine Testes. ,
J Assist Reprod Genet
 31 , 983-91 , 2014
Cells
Testicular
Enzymes
Hyaluronidase : 0.1%
Trypsin : 0.25%
Medium
DMEM
Stem
Porcine
Porcine, male, 8 day
Dirami G, Ravindranath N, Pursel V, Dym M.
Effects of stem cell factor and granulocyte macrophage-colony stimulating factor on survival of porcine type A spermatogonia cultured in KSOM ,
Biol Reprod
 61 , 225-30 , 1999
Cells
Seminiferous epithelial cells
Enzymes
Collagenase : 0.15%
Hyaluronidase : 0.15%
Trypsin : 0.05%
Medium
DMEM/F12
Stem
Rat
Rat
Jiang, H. , Zhang, Y. , Tian, K. , Wang, B. and Han, S.
Amelioration of Experimental Autoimmune Encephalomyelitis Through Transplantation of Placental Derived Mesenchymal Stem Cells. ,
Sci Rep
 7 , 41837 , 2017
Cells
Placental mesenchymal
Enzymes
Medium
DMEM
Stem
Rat
Rat, adult
Duarte, S. , Momier, D. , Baque, P. , Casanova, V. , Loubat, A. , Samson, M. , Guigonis, J. , Staccini, P. , Saint-Paul, M. , De Lima, M. , Carle, G. and Pierrefite-Carle, V.
Preventive Cancer Stem Cell-Based Vaccination Reduces Liver Metastasis Development in a Rat Colon Carcinoma Syngeneic Model. ,
Stem Cells
 31 , 423-32 , 2013
Cells
Tumor
Enzymes
Medium
DMEM/F12
Stem
Rat
Rat, male, 200-250 g
Gobbel GT, Choi SJ, Beier S, Niranjan A.
Long-term cultivation of multipotential neural stem cells from adult rat subependyma ,
Brain Res
 980 , 221 , 2003
Cells
Neural stem cells
Enzymes
Papain : 0.09%
Medium
EBSS
Stem
Rat
Rat, SD, embyronic
Bixby, S. , Kruger, G. , Mosher, J. , Joseph, N. and Morrison, S.
Cell-Intrinsic Differences Between Stem Cells from Different Regions of the Peripheral Nervous System Regulate the Generation of Neural Diversity. ,
Neuron
 35 , 643 , 2002
Cells
Sciatic nerve and gut neural crest stem
Enzymes
Trypsin : 0.005%
Medium
HBSS
Stem
Rat
Rat, adult
Palmer, T. , Markakis, E. , Willhoite, A. , Safar, F. and Gage, F.
Fibroblast Growth Factor-2 Activates a Latent Neurogenic Program in Neural Stem Cells from Diverse Regions of the Adult CNS ,
J Neurosci
 19 , 8487 , 1999
Cells
Neural stem cells
Enzymes
Papain : 2.5 u/ml
Deoxyribonuclease I : 250 u/ml
Neutral Protease : 1 u/ml
Medium
DMEM/F-12
Thymus
Human
Human
Vandenabeele S, Hochrein H, Mavaddat N, Winkel K, Shortman K.
Human thymus contains 2 distinct dendritic cell populations ,
Blood
 97 , 1733-41 , 2001
Cells
Dentritic
Enzymes
Medium
RPMI 1640
Thymus
Mouse
Mouse
Rode, I. and Boehm, T.
Regenerative Capacity of Adult Cortical Thymic Epithelial Cells. ,
Proc Natl Acad Sci U S A
 109 , 3463-8 , 2012
Cells
Thymic stromal
Enzymes
Medium
RPMI-1640
Thymus
Mouse
Mouse
Phillips, Joy, Brondstetter, T. , English, C. , Lee, H. , Virts, E. and Thoman, M.
IL-7 Gene Therapy in Aging Restores Early thymopoiesis without Reversing Involution ,
J Immunol
 , 4869 , 2004
Cells
Stromal
Enzymes
Medium
RPMI 1640
Thymus
Mouse
Mouse
Schiavoni, F, Mattei, F, Sestili, P, Borghi, P, Venditti, M, Morse, H, Belardelli, F. and Gabrieli, L
ICSBP is Essential for the Development of Mouse Type I Interferon-producing Cells and for the Generation and Activation of CD8a+ Dendritic Cells ,
J Exp Med
 196 , 1415 , 2002
Cells
Dentritic
Enzymes
Medium
RPMI 1640
Thyroid/Parathyroid
Bovine
Bovine, adult
Nygren, P. , Gylfe, E. , Larsson, R. , Johansson, H. , Juhlin, C. , Klareskoq, L. , Akerstrom, G. and Rastad, J.
Modulation of the Ca2+-Sensing Function of Parathyroid Cells In Vitro and in Hyperparathyroidism ,
Biochim Biophys Acta
 968 , 253 , 1988
Cells
Parathyroid
Enzymes
Medium
HEPES Ham's F10
Thyroid/Parathyroid
Bovine
Bovine
Wallace, J. and Scarpa, A.
Regulation of Parathyroid Hormone Secretion in Vitro by Divalent Cations ,
J Biol Chem
 257 , 10613 , 1982
Cells
Parathyroid glands
Enzymes
Medium
HEPES buffer
Thyroid/Parathyroid
Bovine
Bovine
Brown, E. , Hurwitz, S. and Aurbach, G.
Preparation of Viable Isolated Bovine Parathyroid Cells ,
Endocrinology
 99 , 1582 , 1976
Cells
Parathyroid
Enzymes
Medium
Eagle's #2 medium without bicarbonate
Thyroid/Parathyroid
Mouse
Mouse
Kitajima, S. , Kohno, S. , Kondoh, A. , Sasaki, N. , Nishimoto, Y. , Li, F. , Mohammed, M. , Muranaka, H. , Nagatani, N. , Suzuki, M. , Kido, Y. and Takahashi, C.
Undifferentiated State Induced by Rb-p53 Double Inactivation in Mouse Thyroid Neuroendocrine Cells and Embryonic Fibroblasts. ,
Stem Cells
 33 , 1657-69 , 2015
Cells
Thyroid tumor
Enzymes
Collagenase Type 1 : 300 u/ml
Hyaluronidase : 100 u/ml
Medium
DMEM
Tonsil
Human
Human, female, 25-45 year
Grammer Amrie C, Slota Rebecca, Fischer Randy, Gur Hanan, Girschick Hermann, Yarboro Cheryl, Illei Gabor G, Lipsky PeterE.
Abnormal germinal center reactions in systemic lupus erythematosus demonstrated by blockade of CD154-CD40 interactions ,
J Clin Invest
 112 , 1506-20 , 2003
Cells
Tonsillar mononuclear cells
Enzymes
Medium
RPMI
Tonsil
Human
Human
Grammer AC, McFarland RD, Heaney J, Darnell BF, Lipsky PE.
Expression, regulation, and function of B cell-expressed CD154 in germinal centers ,
J Immunol
 163 , 4150-9 , 1999
Cells
Tonsillar mononuclear cells
Enzymes
Medium
RPMI
Tumor
Hamster
Hamster, 90-100 g
Gonzalez, A. , Oberley, T. , Schultz, J. , Ostrom, J. and Li, J.
In Vitro Charactrization of Estrogen Induced Syrian Hamster Renal Tumors: Comparison with an Immortalized Cell Line Derived from Diethylstilbestrol-Treated Adult Hamster Kidney ,
In Vitro Cell Dev Biol
 29A , 562 , 1993
Cells
Tumor
Enzymes
Hyaluronidase : 0.1%
Medium
Waymouth's MB
Tumor
Human
Human
Ayuso, J. , Vitek, R. , Swick, A. , Skala, M. , Wisinski, K. , Kimple, R. , Lambert, P. and Beebe, D.
Effects of Culture Method on Response to EGFR Therapy in Head and Neck Squamous Cell Carcinoma Cells ,
Sci Rep
 9 , 12480 , 2019
Cells
Fibroblasts
Enzymes
Medium
FM Fibroblast media
Tumor
Human
Human, 25-81 yr
Volovitz, I, Shapira, N. , Ezer, H. , Gafni, A. , Lustgarten, M. , Alter, T. , Ben-Horin, I. , Barzilai, O. , Shahar, T. , Kanner, A. , Fried, I. , Veshchev, I. , Grossman, R. and Ram, Z,
A Non-Aggressive, Highly Efficient, Enzymatic Method for Dissociation of Human Brain-Tumors and Brain-Tissues to Viable Single-Cells. ,
BMC Neurosci
 17 , 30 , 2016
Cells
Brain tumor
Enzymes
Neutral Protease : 0.11 u/ml
Hyaluronidase : 1,000 u/ml
Medium
HBSS
Tumor
Human
Human
Baldan, V. , Griffiths, R. , Hawkins, R. and Gilham, D
Efficient and Reproducible Generation of Tumour-Infiltrating Lymphocytes for Renal Cell Carcinoma ,
Br J Cancer
 112 , 1510 , 2015
Cells
Tumour infiltrating lymphocytes
Enzymes
Medium
RPMI 1640
Tumor
Human
Human
Quatromoni, J. , Singhal, S. , Bhojnagarwala, P. , Hancock, W. , Albelda, S. and Eruslanov, E.
An Optimized Dissagregation Method for Human Lung Tumors That Perserves the Phenotype and Function of the Immune Cells ,
J Leukoc Biol
 97 , 201 , 2015
Cells
Lung tumor
Enzymes
Collagenase Type 1 : 45-60 u/ml
Collagenase Type 2 : 15-20 u/ml
Collagenase Type 4 : 45-60 u/ml
Elastase : 0.002%
Medium
DMEM/F12
Tumor
Human
Human
Dobbin, Z. and Landen, C.
Isolation and Characterization of Potential Cancer Stem Cells from Solid Human Tumors--Potential Applications. ,
Curr Protoc Pharmacol
 63 , Unit 14.28. , 2013
Cells
Cancer stem
Enzymes
Trypsin : 0.25%
Collagenase : 0.05%
Hyaluronidase : 0.005%
Medium
RPMI
Tumor
Human
Human
Chou, J. , Fitzgibbon, M. , Mortales, C. , Towlerton, A. , Upton, M. , Yeung, R. , McIntosh, M. and Warren, E.
Phenotypic and Transcriptional Fidelity of Patient-Derived Colon Cancer Xenografts in Immune-Deficient Mice. ,
PLoS ONE
 8 , e79874 , 2013
Cells
Tumor
Enzymes
Medium
DMEM/F12
Tumor
Human
Human
Welte, Y. , Davies, C. , Schafer, R. and Regenbrecht, C.
Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma. ,
J Vis Exp
 73 , e50200 , 2013
Cells
Melanoma
Enzymes
Medium
PBS
Tumor
Human
Human
Hussein, D. , Punjaruk, W. , Storer, L. , Shaw, L. , Othman, R. , Ottoman, R. , Peet, A. , Miller, S. , Bandopadhyay, G. , Heath, R. , Kumari, R. , Bowman, K. , Braker, P. , Rahman, R. , Jones, G. , Watson, S. and Lowe, J.
Pediatric Brain Tumor Cancer Stem Cells: Cell Cycle Dynamics, DNA Repair, and Etoposide Extrusion. ,
Neuro Oncol
 13 , 70-83 , 2011
Cells
Brain tumor
Enzymes
Medium
DMEM/F12
Tumor
Human
Human
Zhou, J, Belov, L. , Solomon, M. , Chan, C. , Clarke, S. and Christopherson, R.
Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing. ,
J Vis Exp
 55 , e3322 , 2011
Cells
Colorectal cancer
Enzymes
Medium
RPMI 1640
Tumor
Human
Human
Quintana, E. , Shackleton, M. , Foster, H. , Fullen, D. , Sabel, M. , Johnson, T. and Morrison, S.
Phenotypic Heterogeneity Among Tumorigenic Melanoma Cells from Patients that is Reversible and Not Hierarchically Organized. ,
Cancer Cell
 18 , 510 , 2010
Cells
Tumorigenic melanoma
Enzymes
Collagenase Type 4 : 200 u/ml
Trypsin : 0.05%
Deoxyribonuclease I : 50-100 u/ml
Medium
PBS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.