For our international customers, please be advised that orders cannot be placed through our website by customers in countries with International Distributor representation.

Quick Order

Tissue References Search Tool

Search
Lung
Mouse
Mouse
Kim, H. , Lee, H. , Chang, Y. , Pichavant, M. , Shore, S. , Fitzgerald, K. , Ikawura, Y. , Israel, E. , Bolger, K. , Faul, J. , DeKruyff, R. and Umetsu, D.
IL-17 Producing Innate Lymphoid Cells and the NLRP3 Inflammasone Facilitate Obescity-associated Airway Hyperractivity ,
Nat Med
 20 , 54 , 2014
Cells
Pulmonary
Enzymes
Medium
see reference
Lung
Mouse
Mouse
Misharin, A. , Morales-Nebreda, L. , Mutlu, G. , Budinger, G. and Perlman, H.
Flow Cytometric Analysis of Macrophages and Dendritic Cell Subsets in the Mouse Lung. ,
Am J Respir Cell Mol Biol
 49 , 503-10 , 2013
Cells
Lung
Enzymes
Medium
HBSS
Lung
Mouse
Mouse
Vaughan, A. , Halbert, C. , Wootton, S. and Miller, A.
Lung Cancer in Mice Induced by the Jaagsiekte Sheep Retrovirus Envelope Protein is not Maintained by Rare Cancer Stem Cells, but Tumorigenicity does Correlate with Wnt Pathway Activation. ,
Mol Cancer Res
 10 , 86 , 2012
Cells
Lung tumor
Enzymes
Neutral Protease : 50 u/ml
Collagenase : 400 u/ml
Medium
DMEM
Lung
Mouse
Mouse
Hardy, C. , LeMasurier, J. , Belz, G. , Scalzo-Inguanti, K. , Yao, J. , Xiang, S. , Kanellakis, P. , Bobik, A. , Strickland, D. , Rolland, J. , O'Hehir, R. and Plebanski, M.
Inert 50-nm Polystyrene Nanoparticles That Modify Pulmonary Dendritic Cell Function and Inhibit Allergic Airway Inflammation. ,
J Immunol
 188 , 1431 , 2012
Cells
Lung
Enzymes
Medium
RPMI 1640
Lung
Mouse
Mouse, 8-12 week
Driscoll, B. , Kikuchi, A. , Lau, A. , Lee, J. , Reddy, R. , Jesudason, E. , Kim, C. and Warburton, D.
Isolation and Characterization of Distal Lung Progenitor Cells. ,
Methods Mol Biol
 879 , 109 , 2012
Cells
Lung progenitor
Enzymes
Medium
DMEM
Lung
Mouse
Mouse
Rock, J, Barkauskas, C, Cronc, M, Xue, Y, Harris, J, Liang, J, Noble, P. and Hogan, B.
Multiple Stromal Populations Contribute to Pulmonary Fibrosis Without Evidence for Epithelial to Mesenchymal Transition. ,
Proc Natl Acad Sci U S A
 108 , E1475 , 2011
Cells
Lung
Enzymes
Collagenase Type 1 : 450 u/ml
Elastase : 4 u/ml
Neutral Protease : 5 u/ml
Deoxyribonuclease I : 0.33 u/ml
Medium
DMEM/F12
Lung
Mouse
Mouse
Lancelin, W. and Guerrero-Plata, A.
Isolation of Mouse Lung Dendritic Cells. ,
J Vis Exp
 57 , e3563 , 2011
Cells
Dendritic
Enzymes
Medium
HBSS
Lung
Mouse
Mouse, female, 6-12 week
Breslow, R. , Rao, J. , Xing, W. , Hong, D. , Barrett, N. and Katz, H.
Inhibition of Th2 Adaptive Immune Responses and Pulmonary Inflammation by Leukocyte Ig-Like Receptor B4 on Dendritic Cells. ,
J Immunol
 184 , 1003 , 2010
Cells
Lung mononuclear
Enzymes
Medium
HBSS
Lung
Mouse
Mouse
Ferreira, C. , Antunes, F. , Leonard, V. , Welstead, G. , Richardson, C. and Cattaneo, R.
Measles Virus Infection of Alveolar Macrophages and Dendritic Cells Precedes Spread to Lymphatic Organs in Transgenic Mice Expressing Human Signaling Lymphocytic Activation Molecule (SLAM, CD150). ,
J Virol
 84 , 3033 , 2010
Cells
Lung
Enzymes
Medium
RPMI 1640
Lung
Mouse
Mouse, 6-7 week
Jungblut, M. , Oeltze, K. , Zehnter, I. , Hasselmann, D. and Bosio, A.
Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue Using the GentleMACS Dissociator. ,
J Vis Exp
 29 , 2009
Cells
Leukocytes and endothelial
Enzymes
Collagenase : 0.2%
Deoxyribonuclease I : 40-80 u/ml
Medium
PBS
Lung
Mouse
Mouse
Zhao, J. , Zhao, J. , Van Rooijen, N. and Perlman, S.
Evasion by Stealth: Inefficient Immune Activation Underlies Poor T Cell Response and Severe Disease in SARS-CoV-Infected Mice. ,
PLoS Pathog
 5 , e1000636 , 2009
Cells
Lung
Enzymes
Medium
HBSS
Lung
Mouse
Mouse, embryo, day 18
Trotter, A. , Kipp, M. , Schrader, R. and Beyer, C.
Combined Application of 17Beta-Estradiol and Progesterone Enhance Vascular Endothelial Growth Factor and Surfactant Protein Expression in Cultured Embryonic Lung Cells of Mice. ,
Int J Pediatr
 2009 , 170491 , 2009
Cells
Alveolar and fibroblast
Enzymes
Trypsin : 2.5%
Collagenase Type 1 : 1250 u/ml
Medium
MEM
Lung
Mouse
Mouse
Finotto, S. , Eigenbrod, T. , Karwot, R. , Boross, I. , Doganci, A. , Ito, H. , Nishimoto, N. , Yoshizaki, K. , Kishimoto, T. , Rose-John, S. , Galle, P. and Neurath, M.
Local Blockade of IL-6R Signaling Induces Lung CD4+ T Cell Apoptosis in a Murine Model of Asthma Via Regulatory T Cells. ,
Int Immunol
 19 , 685 , 2007
Cells
Lung
Enzymes
Medium
Dulbecco's PBS
Lung
Mouse
Mouse, 8-12 week
Woolard MD, Hodge LM, Jones HP, Schoeb TR. and Simecka JW
The upper and lower respiratory tracts differ in their requirement of IFN-gamma and IL-4 in controlling respiratory mycoplasma infection and disease ,
J Immunol
 172 , 6875 , 2004
Cells
Mononuclear cells
Enzymes
Medium
RPMI 1640 medium
Lung
Mouse
Mouse
Paine R 3rd, Wilcoxen SE, Morris SB, Sartori C, Baleeiro CE, Matthay MA. and Christensen PJ
Transgenic overexpression of granulocyte macrophage-colony stimulating factor in the lung prevents hyperoxic lung injury ,
Am J Pathol
 163 , 2397 , 2003
Cells
Alveolar epithelial cells
Enzymes
Medium
DMEM
Lung
Mouse
Mouse
Huaux F, Liu T, McGarry B, Ullenbruch M. and Phan SH.
Dual roles of IL-4 in lung injury and fibrosis ,
J Immunol
 170 , 2083 , 2003
Cells
Pulmonary T lymphocytes
Enzymes
Collagenase Type 3 : 10 mg/lung
Deoxyribonuclease I : 250 ug/lung
Medium
RPMI medium
Lung
Mouse
Mouse, 6-8 week
Vermaelen KY, Carro-Muino I, Lambrecht BN. and Pauwels RA.
Specific migratory dendritic cells rapidly transport antigen from the airways to the thoracic lymph nodes ,
J Exp Med
 193 , 51 , 2001
Cells
Lung and lymph node cells
Enzymes
Medium
RPMI 1640
Lung
Mouse
Mouse
Freedman SD, Katz MH, Parker EM, Laposata M, Urman MY. and Alvarez JG.
A membrane lipid imbalance plays a role in the phenotypic expression of cystic fibrosis in cftr(-/-) mice ,
Proc Natl Acad Sci U S A
 96 , 13995 , 1999
Cells
Lung
Enzymes
Collagenase : 100 u/ml
Deoxyribonuclease I : 200 u/ml
Medium
Krebs-Henseleit Buffer
Lung
Mouse
Mouse, female
Hamilton-Easton A. and Eichelberger M
Virus-specific antigen presentation by different subsets of cells from lung and mediastinal lymph node tissues of influenza virus-infected mice ,
J Virol
 69 , 6359 , 1995
Cells
Antigen presenting cells
Enzymes
Collagenase : 150 u/ml
Medium
RPMI 1640
Lymph nodes
Mouse
Mouse
Terhorst, D. , Chelbi, R. , Wohn, C. , Malosse, C. , Tamoutounour, S. , Jorquera, A. , Bajenoff, M. , Dalod, M. , Malissen, B. and Henri, S.
Dynamics and Transcriptomics of Skin Dendritic Cells and Macrophages in an Imiquimod-Induced, Biphasic Mouse Model of Psoriasis. ,
J Immunol
 195 , 4953-61 , 2015
Cells
Dendritic
Enzymes
Medium
RPMI 1640
Lymph nodes
Mouse
Mouse
Dubrot, J. , Duraes, F. , Potin, L. , Capotosti, F. , Brighouse, D. , Suter, T. , LeibundGut-Landmann, S. , Garbi, N. , Reith, W. , Swartz, M. and Hugues, S.
Lymph Node Stromal Cells Acquire Peptide-MHCII Complexes from Dendritic Cells and Induce Antigen-Specific CD4+T Cell Tolerance ,
J Exp Med
 211 , 1153 , 2014
Cells
Stromal
Enzymes
Medium
RPMI
Lymph nodes
Mouse
Mouse
Kapasi ZF, Qin D, Kerr WG, Kosco-Vilbois MH, Shultz LD, Tew JG, Szakal AK.
Follicular dendritic cell (FDC) precursors in primary lymphoid tissues ,
J Immunol
 160 , 1078-84 , 1998
Cells
Follicular dentritic
Enzymes
Medium
HBSS
Mammary
Mouse

Mouse, female

Sun, H. , Xu, X. and Deng, C.
Preparation of Single Epithelial Cells Suspension from Mouse Mammary Glands. ,
Bio Protoc
 10 , e3530 , 2020
Cells
Mammary epithelial
Enzymes
Medium
DMEM/F-12
Mammary
Mouse
Mouse, 10 week
Cheng, N. and Lambert, D.
Mammary Transplantation of Stromal Cells and Carcinoma Cells in C57BL/6J Mice. ,
J Vis Exp
 54 , e2716 , 2011
Cells
Fibroblasts, carcinoma
Enzymes
Trypsin : 0.2%
Hyaluronidase : 1,000 u/ml
Medium
DMEM
Mammary
Mouse
Mouse, 12 week
Taddei, I. , Deugnier, M. , Faraldo, M. , Petit, V. , Bouvard, D. , Medina, D. , Fassler, R. , Thiery, J. and Glukhova, M.
Beta1 Integrin Deletion from the Basal Compartment of the Mammary Epithelium Affects Stem Cells ,
Nat Cell Biol
 10 , 716-22 , 2008
Cells
Mammary epithelial
Enzymes
Collagenase : 0.3%
Hyaluronidase : 100 u/ml
Trypsin : 0.25%
Medium
see reference
Mammary
Mouse
Mouse, 11 week
Mueller, S. , Clark, J. , Myers, P. and Korach, K.
Mammary Gland Development in Adult Mice Requires Epithelial and Stromal Estrogen Receptor Alpha. ,
Endocrinology
 143 , 2357 , 2002
Cells
Epithelial
Enzymes
Collagenase Type 3 : 25 u/ml
Hyaluronidase : 0.1%
Protease XIV : 0.05%
Medium
DMEM/F12
Mammary
Mouse
Mouse
Boulanger CA, Smith GH.
Reducing mammary cancer risk through premature stem cell senescence ,
Oncogene
 20 , 2264-72 , 2001
Cells
Mammary epithelial stem
Enzymes
Hyaluronidase : 0.1%
Pronase : 1.25%
Medium
DMEM
Mammary
Mouse
Mouse (C3H/HeN), female
Taketani, Y. and Oka, T.
EGF Stimulates Cell Proliferation and Inhibits Functional Differentiation of Mouse Mammalian Dipithelial Cells in Culture ,
Endocrinology
 113 , 871 , 1983
Cells
Epithelial
Enzymes
Medium
Medium 199
Mammary
Mouse
Mouse, female
Jones, W. and Hallowes, R.
Isolation of the Epithelial Subcomponents of the Mouse Mammary Gland for Tissue-Level Culture Studies ,
J Tiss Cul Meth
 8 (1) , 17 , 1983
Cells
Epithelial
Enzymes
Medium
DMEM
Miscellaneous
Mouse

Mouse

Hameed, M. , Rai, P. , Makris, M. and Weger-Lucarelli, J.
Optimized Protocol for Mouse Footpad Immune Cell Isolation for Single-cell RNA Sequencing and Flow Cytometry. ,
Star Protocols
 4 , 102409 , 2023
Cells
Leucocytes
Enzymes
Medium
RPMI-1640
Miscellaneous
Mouse
Mouse, embryonic
He, M. , Wu, B. , Ye, W. , Le, D. , Sinclair, A. , Padovano, V. , Chen, Y. , Li, K. , Sit, R. , Tan, M. , Caplan, M. , Neff, N. , Jan, Y. , Darmanis, S. and Jan, L.
Chloride Channels Regulate Differentiation and Barrier Functions of the Mammalian Airway. ,
Elife
 9 , 53085 , 2020
Cells
Trachea
Enzymes
Elastase : 0.1%
Trypsin : 0.125%
Medium
DMEM/F12
Miscellaneous
Mouse
Mouse, 8-12 week
Atta, H. , Gheinani, A. , Wacker, A. , Heshmati, Y. , Bigger-Allen, A. , Lambrinos, G. , Gao, Y. , Bielenberg, D. and Adam, R.
A Single Cell Dissociation Approach for Molecular Analysis of Urinary Bladder in the Mouse Following Spinal Cord Injury. ,
J Vis Exp
 , 2020
Cells
Urinary bladder
Enzymes
Collagenase Type 1 : 50 u/ml
Collagenase Type 3 : 40 u/ml
Papain : 45 u/ml
Collagenase Type 6 : 20 u/ml
Medium
Tyrode solution
Miscellaneous
Mouse
Mouse, 10 week
Futami, I. , Ishijima, M. , Kaneko, H. , Tsuji, K. , Ichikawa-Tomikawa, N. , Sadatsuki, R. , Muneta, T. , Arikawa-Hirasawa, E. , Sekiya, I. and Kaneko, K.
Isolation and Characterization of Multipotential Mesenchymal Cells from the Mouse Synovium. ,
PLoS ONE
 7 , e45517 , 2012
Cells
Synovial mesenchymal
Enzymes
Medium
DMEM
Miscellaneous
Mouse
Mouse
Stock, A. , Booth, S. and Cerundolo, V.
Prostaglandin E2 Suppresses the Differentiation of Retinoic Acid-Producing Dendritic Cells in Mice and Humans. ,
J Exp Med
 208 , 761 , 2011
Cells
Dentritic
Enzymes
Medium
DME
Miscellaneous
Mouse
Mouse
Shi, C. , Jia, T. , Mendez-Ferrer, S. , Hohl, T. , Serbina, N. , Lipuma, L. , Leiner, I. , Li, M. , Frenette, P. and Pamer, E.
Bone Marrow Mesenchymal Stem and Progenitor Cells Induce Monocyte Emigration in Response to Circulating Toll-Like Receptor Ligands. ,
Immunity
 34 , 590 , 2011
Cells
Spleen, bone marrow endothelial
Enzymes
Medium
PBS
Miscellaneous
Mouse
Mouse, 6-10 week
Ji, H. , Rintelen, F. , Waltzinger, C. , Meier, D, Bilancio, A. , Pearce, W. , Hirsch, E. , Wymann, M. , Ruckle, T. , Camps, M. , Vanhaesebroeck, B. , Okkenhaug, K. and Rommel, C.
Inactivation of PI3Kgamma and PI3Kdelta Distorts T-cell Development and Causes Multiple Organ Inflammation. ,
Blood
 110 , 2940 , 2007
Cells
Salivary gland and stomach
Enzymes
Medium
RPMI-1640

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.