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Tissue References Search Tool

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Brain
Human
Human
Scherpelz, K. , Lu, J.,Tycko, R. and Meredith, S.
Preparation of Amyloid Fibrils Seeded from Brain and Meninges ,
Methods Mol Biol
 1345 , 299 , 2016
Cells
Amyloid Fibrils
Enzymes
Medium
50mM Tris
Brain
Human
Human, 25-81 yr
Volovitz, I, Shapira, N. , Ezer, H. , Gafni, A. , Lustgarten, M. , Alter, T. , Ben-Horin, I. , Barzilai, O. , Shahar, T. , Kanner, A. , Fried, I. , Veshchev, I. , Grossman, R. and Ram, Z,
A Non-Aggressive, Highly Efficient, Enzymatic Method for Dissociation of Human Brain-Tumors and Brain-Tissues to Viable Single-Cells. ,
BMC Neurosci
 17 , 30 , 2016
Cells
Brain tumor
Enzymes
Neutral Protease : 0.11 u/ml
Hyaluronidase : 1,000 u/ml
Medium
HBSS
Brain
Human
Hussein, D. , Punjaruk, W. , Storer, L. , Shaw, L. , Othman, R. , Ottoman, R. , Peet, A. , Miller, S. , Bandopadhyay, G. , Heath, R. , Kumari, R. , Bowman, K. , Braker, P. , Rahman, R. , Jones, G. , Watson, S. and Lowe, J.
Pediatric Brain Tumor Cancer Stem Cells: Cell Cycle Dynamics, DNA Repair, and Etoposide Extrusion. ,
Neuro Oncol
 13 , 70-83 , 2011
Cells
Brain tumor
Enzymes
Medium
DMEM/F12
Brain
Human
Human
Sauvageot, C. , Weatherbee, J. , Kesari, S. , Winters, S. , Barnes, J. , Dellagatta, J. , Ramakrishna, N. , Stiles, C. , Kung, A. , Kieran, M. and Wen, P.
Efficacy of the HSP90 Inhibitor 17-AAG in Human Glioma Cell Lines and Tumorigenic Glioma Stem Cells. , Neuro Oncol Vol. 11 , 109 , 2009
Cells
Tumor
Enzymes
Medium
see reference
Brain
Human
Human
Klegeris Andis, McGeer PatrickL.
Chymotrypsin-like proteases contribute to human monocytic THP-1 cell as well as human microglial neurotoxicity ,
Glia
 51 , 56-64 , 2005
Cells
Microglia
Enzymes
Trypsin : 0.25%
Medium
DMEM/F12
Brain
Human
Human
Roher, A.E. , Palmer, K.C. , Chau, V. and Ball, M.J.
Isolation and Chemical Characterization of Alzheimer's Disease Paired Helical Filament Cytoskeletons:Differentiation from Amyloid Plaque Core Protein ,
J Cell Biol
 107 , 2703 , 1988
Cells
Neuronal
Enzymes
Deoxyribonuclease I : 10 µg/ml
Medium
Tris-HCl, 50 mM, CaCl2, 2 mM
Brain
Monkey
Monkey, rhesus
Marcondes MC, Burudi EM, Huitron-Resendiz S, Sanchez-Alavez M, Watry D, Zandonatti M, Henriksen SJ, Fox HS.
Highly activated CD8(+) T cells in the brain correlate with early central nervous system dysfunction in simian immunodeficiency virus infection ,
J Immunol
 167 , 5429-38 , 2001
Cells
Brain cells
Enzymes
Medium
HBSS
Brain
Mouse
Adult and neonatal
Calvo, Belen, Rubio, F. , Fernandez, M. and Tranque, P.
Dissociation of Neonatal and Adult Mice Brain for Simultaneous Analysis of Microglia, Astrocytes and Infiltrating Lymphocytes by Flow Cytometry. , IBRO Rep Vol. 8 , 36-47 , 2020
Cells
Microglia, astrocytes, lymphocytes
Enzymes
Collagenase Type 2 : 120 u/ml
Papain : 20 u/ml
Neutral Protease : 1.2 u/ml
Medium
EBSS
Brain
Mouse
Mouse
Kramer, D. , Risso, D. , Kosillo,. , Ngai, J. and Bateup, H.
Combinatorial Expression of Grp and Neurod6 Defines Dopamine Neuron Populations with Distinct Projection Patterns and Disease Vulnerability , eNeuro Vol. 5 , 1 , 2018
Cells
Neurons
Enzymes
Papain : 10 u/ml
Medium
Artificial CSF
Brain
Mouse
Mouse, P13-15
Haseleu, J. , Anlauf, E. , Blaess, S. , Endl, E. and Derouiche, A.
Studying Subcellular Detail in Fixed Astrocytes: Dissociation of Morphologically Intact Glial Cells (DIMIGs). ,
Front Cell Neurosci
 7 , 54 , 2013
Cells
Glial
Enzymes
Papain : 20 u/ml
Medium
EBSS
Brain
Mouse
Mouse, P0-P15
Foo, L.
Purification of Rat and Mouse Astrocytes by Immunopanning. ,
Cold Spring Harb. Protoc.
 2013 , 421-32 , 2013
Cells
Astrocytes
Enzymes
Papain : 5 u/ml
Medium
DPBS
Brain
Mouse
Mouse
Shah. G. , Price, T. , Banks, W. , Morofuji, Y. , Kovac, A. , Ercal, N. , Sorenson, C. , Shin, E. and Sheibani, N.
Pharmacological Inhibition of Mitochondrial Carbonic Anhydrases Protects Mouse Cerebral Pericytes from High Glucose-Induced Oxidative Stress and Apoptosis. ,
J Pharmacol Exp Ther
 344 , 637-45 , 2013
Cells
Cerebral pericytes
Enzymes
Medium
DMEM
Brain
Mouse
Mouse
Ryg-Cornejo, V. , Ioannidis, L. and Hansen, D.
Isolation and Analysis of Brain-Sequestered Leukocytes from Plasmodium Berghei ANKA-Infected Mice. ,
J Vis Exp
 71 , e50112 , 2013
Cells
Brain-sequestered leukocytes
Enzymes
Medium
RPMI
Brain
Mouse
Mouse, 1-2 day
O'Meara, R. , Ryan, S. , Colognato, H. and Kothary, R.
Derivation of Enriched Oligodendrocyte Cultures and Oligodendrocyte/Neuron Myelinating Co-Cultures from Post-Natal Murine Tissues. ,
J Vis Exp
 54 , 3324 , 2011
Cells
Oligodendrocytes, dorsal root ganglia
Enzymes
Papain : 0.15%
Medium
DMEM
Brain
Mouse
Mouse, postnatal
Sher, F. , Rossler, R. , Brouwer, N. , Balasubramaniyan, V. , Boddeke, E. and Copray, S.
Differentiation of Neural Stem Cells Into Oligodendrocytes: Involvement of the Polycomb Group Protein Ezh2. ,
Stem Cells
 26 , 2875 , 2008
Cells
Astrocytes
Enzymes
Trypsin : 0.25%
Deoxyribonuclease I : 1,000 u/ml
Medium
HBSS
Brain
Mouse
Mouse
Oliver, T. , Read, T. , Kessler, J. , Mehmeti, A. , Wells, J. , Huynh, T. , Lin, S. and Wechsler-Reya, R.
Loss of Patched and Disruption of Granule Cell Development in a Pre-Neoplastic Stage of Medulloblastoma. ,
Development
 132 , 2425 , 2005
Cells
Granule cell precursors, pre-neoplastic and tumor cells
Enzymes
Papain : 10 u/ml
Deoxyribonuclease I : 250 u/ml
Medium
Neurobasal/B27
Brain
Mouse
Mouse, 3 day
Nishioku T, Hashimoto K, Yamashita K, Liou SY, Kagamiishi Y, Maegawa H, Katsube N, Peters C, von Figura K, Saftig P, Katunuma N, Yamamoto K, Nakanishi H.
Involvement of cathepsin E in exogenous antigen processing in primary cultured murine microglia ,
J Biol Chem
 277 , 4816 , 2002
Cells
Microglia
Enzymes
Papain : 90 u/ml
Deoxyribonuclease I : 2000 u/ml
Medium
Eagle's MEM
Brain
Mouse
Mouse, SD, 8-12 week
O'Donnell SL, Frederick TJ, Krady JK, Vannucci SJ, Wood TL.
IGF-I and microglia/macrophage proliferation in the ischemic mouse brain ,
Glia
 39 , 85 , 2002
Cells
Microglia
Enzymes
Medium
RPMI-1640
Brain
Mouse
Mouse
Miyazawa K, Himi T, Garcia V, Yamagishi H, Sato S. and Ishizaki Y.
A role for p27/Kip1 in the control of cerebellar granule cell precursor proliferation ,
J Neurosci
 20 , 5756 , 2000
Cells
Cerebellar granule cell precursors
Enzymes
Papain : 0.435%
Medium
EBSS
Brain
Ovine
Ovine
Reddy, R. , Amodei, R. , Estill, C. , Stormshak, F. , Meaker, M. and Roselli, C.
Effect of Testosterone on Neuronal Morphology and Neuritic Growth of Fetal Lamb Hypothalamus-Preoptic Area and Cerebral Cortex in Primary Culture. ,
PLoS ONE
 10 , e0129521 , 2015
Cells
Neurons
Enzymes
Papain : 0.2%
Medium
Hibernate
Brain
Rat
Rat, P0-P15
Foo, L.
Purification of Rat and Mouse Astrocytes by Immunopanning. ,
Cold Spring Harb. Protoc.
 2013 , 421-32 , 2013
Cells
Astrocytes
Enzymes
Papain : 5 u/ml
Medium
DPBS
Brain
Rat
Rat, SD, E18
Behar, T, Smith, S, Kennedy, R, Mckenzie, J, Maric, I. and Barker, J
GABA(B) receptors mediate motility signals for migrating embryonic cortical cells ,
Cereb Cortex
 11 , 744-53 , 2001
Cells
Cortical cells
Enzymes
Papain : 20 u/ml
Medium
EBSS
Brain
Rat
Rat, SD, 5 day
Mattson, M. and Kater, S.
Development and Selective Neurodegeneration in Cell Cultures From Different Hippocampal Regions ,
Brain Res
 490 , 110 , 1989
Cells
Hippocampal neurons
Enzymes
Trypsin : 0.2%
Deoxyribonuclease I : 10 ug/ml
Medium
HBSS
Brain
Rat
Rat, SD, fetal
Peterfreund, R. and Vale, W.
High Molecular Weight Somatostatin Secretion by Cultured Rat Brain Cell ,
Brain Res
 239 , 463 , 1982
Cells
Cerebral cortex
Hypothalmus
Enzymes
Medium
HEPES
Mammary
Bovine
Bovine, lactating Holstein
Miranda, S. , Wang, Y. , Purdie, N. , Osborne, V. , Coomber, B. and Cant, J.
Selenomethionine Stimulates Expression of Glutathione Peroxidase 1 and 3 and Growth of Bovine Mammary Epithelial Cells in Primary Culture. ,
J Dairy Sci
 92 , 2670 , 2009
Cells
Mammary epithelial
Enzymes
Collagenase Type 3 : 400 u/ml
Hyaluronidase : 100 u/ml
Medium
HBSS
Mammary
Bovine
Bovine, heifer, 200kg
Weber M. , Purup S. , Vestergaard M. , Ellis S. , Scndergard-Andersen J. , Akers R. and Sejrsen K.
Contribution of Insulin-like Growth Factor (IGF)-I and IGF-binding Protein-3 to Mitogenic Activity in Bovine Mammary Extracts and Serum ,
J Endocrinol
 161 , 365 , 1999
Cells
Mammary epithelial
Enzymes
Medium
M-199
Mammary
Bovine
Bovine
Baumrucker, C. , Deemer, K. , Walsh, R. , Riss, T. and Akers, R.
Primary Culture of Bovine Mammary Acini on a Collagen Matrix ,
Tissue Cell
 20 (4) , 541 , 1988
Cells
Epithelial
Enzymes
Medium
HBSS/Medium 199
Mammary
Mouse

Mouse, female

Sun, H. , Xu, X. and Deng, C.
Preparation of Single Epithelial Cells Suspension from Mouse Mammary Glands. ,
Bio Protoc
 10 , e3530 , 2020
Cells
Mammary epithelial
Enzymes
Medium
DMEM/F-12
Mammary
Mouse
Mouse, 10 week
Cheng, N. and Lambert, D.
Mammary Transplantation of Stromal Cells and Carcinoma Cells in C57BL/6J Mice. ,
J Vis Exp
 54 , e2716 , 2011
Cells
Fibroblasts, carcinoma
Enzymes
Trypsin : 0.2%
Hyaluronidase : 1,000 u/ml
Medium
DMEM
Mammary
Mouse
Mouse, 12 week
Taddei, I. , Deugnier, M. , Faraldo, M. , Petit, V. , Bouvard, D. , Medina, D. , Fassler, R. , Thiery, J. and Glukhova, M.
Beta1 Integrin Deletion from the Basal Compartment of the Mammary Epithelium Affects Stem Cells ,
Nat Cell Biol
 10 , 716-22 , 2008
Cells
Mammary epithelial
Enzymes
Collagenase : 0.3%
Hyaluronidase : 100 u/ml
Trypsin : 0.25%
Medium
see reference
Mammary
Mouse
Mouse, 11 week
Mueller, S. , Clark, J. , Myers, P. and Korach, K.
Mammary Gland Development in Adult Mice Requires Epithelial and Stromal Estrogen Receptor Alpha. ,
Endocrinology
 143 , 2357 , 2002
Cells
Epithelial
Enzymes
Collagenase Type 3 : 25 u/ml
Hyaluronidase : 0.1%
Protease XIV : 0.05%
Medium
DMEM/F12
Mammary
Mouse
Mouse
Boulanger CA, Smith GH.
Reducing mammary cancer risk through premature stem cell senescence ,
Oncogene
 20 , 2264-72 , 2001
Cells
Mammary epithelial stem
Enzymes
Hyaluronidase : 0.1%
Pronase : 1.25%
Medium
DMEM
Mammary
Mouse
Mouse (C3H/HeN), female
Taketani, Y. and Oka, T.
EGF Stimulates Cell Proliferation and Inhibits Functional Differentiation of Mouse Mammalian Dipithelial Cells in Culture ,
Endocrinology
 113 , 871 , 1983
Cells
Epithelial
Enzymes
Medium
Medium 199
Mammary
Mouse
Mouse, female
Jones, W. and Hallowes, R.
Isolation of the Epithelial Subcomponents of the Mouse Mammary Gland for Tissue-Level Culture Studies ,
J Tiss Cul Meth
 8 (1) , 17 , 1983
Cells
Epithelial
Enzymes
Medium
DMEM
Mammary
Rat
Rat, SD
Brake P. , Zhang L. and Jefcoate C.
Aryl Hydrocarbon Receptor Regulation of Cytochrome P4501B1 in Rat Mammary Fibroblasts: Evidence for Transcriptional Repression by Glucocorticoids ,
Mol Pharmacol
 54 , 825 , 1998
Cells
Mammary fibroblasts
Enzymes
Medium
DMEM/F-12

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.