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Tissue References Search Tool

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Epithelial
Human
Human
Fukushima, K. and Fiocchi, C.
Paradoxical Decrease of Mitochondrial DNA Deletions in Epithelial Cells of Active Ulcerative Colitis Patients. ,
Am J Physiol Gastrointest Liver Physiol
 286 , G804-13 , 2004
Cells
Colonic epithelial
Enzymes
Medium
RPMI 1640
Epithelial
Human
Human
Widdicombe, J.H. , Coleman, D.L. , Finkbeiner, W.E. and Tuet, I.K.
Electrical Properties of Monolayers Cultured From Cells of Human Tracheal Mucosa ,
J Appl Physiol
 58 , 1729 , 1985
Cells
Epithelial
Enzymes
Medium
HEPES with 5.9mM Glucose,5mM DTT
Epithelial
Mouse
Mouse, 8-12 week
Brann, D. , Tsukahara, T. , Weinreb, C. , Lipovsek, M. , Van den Berge, K. , Gong, B. , Chance, R. , Macaulay, I. , Chou, H. , Fletcher, R. , Das, D. , Street, K. , de Bezieux, H. , Choi, Y. , Risso, D. and Dudoit, S.
Non-Neuronal Expression of SARS-CoV-2 Entry Genes in the Olfactory System Suggests Mechanisms Underlying COVID-19-Associated Anosmia. ,
Sci. Adv.
 6 , 2020
Cells
Nasal epithelium
Enzymes
Papain : 20 u/ml
Medium
EBSS
Epithelial
Mouse
Mouse
Sun, F. , Xiao, G. and Qu, Z.
Isolation of Murine Alveolar Type II Epithelial Cells. ,
Bio-protocol
 7 , 2017
Cells
Alveolar epithelial
Enzymes
Medium
DMEM
Epithelial
Mouse
Mouse, 6-8 week
Wirtz S. , Becker C. , Blumberg R. , Galle P. and Neurath M.
Treatment of T cell-dependent experimental colitis in SCID mice by local administration of an adenovirus expressing IL-18 antisense mRNA ,
J Immunol
 168(1) , 411 , 2002
Cells
Lamina propria mononuclear cells
Enzymes
Medium
RPMI
Epithelial
Mouse
Mouse, 11 week
Mueller, S. , Clark, J. , Myers, P. and Korach, K.
Mammary Gland Development in Adult Mice Requires Epithelial and Stromal Estrogen Receptor Alpha. ,
Endocrinology
 143 , 2357 , 2002
Cells
Epithelial
Enzymes
Collagenase Type 3 : 25 u/ml
Hyaluronidase : 0.1%
Protease XIV : 0.05%
Medium
DMEM/F12
Epithelial
Rat
Rat, embryonic
Shannon JM, Gebb SA. and Nielsen LD
Induction of alveolar type II cell differentiation in embryonic tracheal epithelium in mesenchyme-free culture ,
Development
 126 , 1675 , 1999
Cells
Tracheal epithelial
Enzymes
Medium
DMEM/F12
Epithelial
Rat
Rat, SD, 200 g
Yassin, R. , Clearfield, H. , Katz, S. and Murthy, S.
Gastrin Induction of mRNA Expression in Rat Colonic Epithelium In Vitro ,
Peptides
 12 , 63 , 1991
Cells
Colon
Enzymes
Deoxyribonuclease I : 10 µg/ml
Medium
(see reference)
Eye
Bovine
Bovine
Terlizzi, V. , Kolibabka, M. , Burgess, J. , Hammes, H. and Harmsen, M.
The Pericytic Phenotype of Adipose Tissue-Derived Stromal Cells Is Promoted by NOTCH2. ,
Stem Cells
 36 , 240-251 , 2018
Cells
Retinal endothelial
Enzymes
Pronase : 0.02%
Medium
DMEM
Eye
Chicken
Chick, embryo, 6 day
Jacob Vanessa, Rothermel Andrae, Wolf Peter, Layer Paul G.
Rhodopsin, violet and blue opsin expressions in the chick are highly dependent on tissue and serum conditions ,
Cell Death Differ
 180 , 159-68 , 2005
Cells
Retinal cells
Enzymes
Trypsin : 0.005%
Medium
DMEM/F12
Eye
Monkey
Macaques and baboon
Han ,Y. , Jacoby, R. and Wu, S.
Morphological and Electrophysiological Properties of Dissociated Primate Retinal Cells. ,
Brain Res
 875 , 175 , 2000
Cells
Retinal
Enzymes
Papain : 20-40 u/ml
Deoxyribonuclease I : 400 u/ml
Medium
Ames' solution
Eye
Mouse

Mouse, 2 month

Murenu, E. , Pavlou, M. , Richter, L. , Rapti, K. , Just, S. and Cehajic-Kapetanovic, J.
A Universal Protocol for Isolating Retinal ON Bipolar Cells Across Species via Fluorescence-Activated Cell Sorting. , Mol Ther Methods Clin Dev Vol. 20 , 587-600 , 2021
Enzymes
Papain : 20 u/ml
Medium
EBSS
Eye
Mouse
Mouse
Ortin-Martinez, A. , Tsai, E. , Nickerson, P. , Bergeret, M. , Lu, Y. , Smiley, S. , Comanita, L. and Wallace, V.
A Reinterpretation of Cell Transplantation: GFP Transfer From Donor to Host Photoreceptors. ,
Stem Cells
 35 , 932-939 , 2017
Cells
Retinal
Enzymes
Papain : 20 u/ml
Medium
PBS
Eye
Mouse
Mouse
Wohl, S. , Jorstad, N. , Levine, E. and Reh, T.
Muller Glial microRNAs are Required for the Maintenance of Glial Homeostasis and Retinal Architecture. ,
Nat Commun
 8 , 1603 , 2017
Cells
Retinal
Enzymes
Papain : 20 u/ml
Medium
HBSS
Eye
Mouse
Mouse, C57BL/6
Blandford, S. and Baldridge, W.
The Effect of Glutamate Receptor Agonists on Mouse Retinal Astrocyte [Ca(2+)]i. ,
Biomed Res Int
 2016 , 8178162 , 2016
Cells
Retinal astrocytes
Enzymes
Papain : 16.5 u/ml
Medium
HBSS
Eye
Mouse
Mouse, embryonic and P0
Dvoriantchikova, G. , Perea-Martinez, I. , Pappas, S. , Barry, A. , Danek, D. , Dvoriantchikova, X. , Pelaez, D. and Ivanov, D.
Molecular Characterization of Notch1 Positive Progenitor Cells in the Developing Retina. ,
PLoS ONE
 10 , e0131054 , 2015
Cells
Retinal
Enzymes
Papain : 8 u/ml
Deoxyribonuclease I : 124 u/ml
Medium
DPBS
Eye
Mouse
Mouse
Amadi-Obi, A. , Yu, C. , Liu, X. , Mahdi, R. , Clarke, G. , Nussenblatt, R, Gery, I. , Lee, Y. and Egwuagu, C.
TH17 Cells Contribute to Uveitis and Scleritis and are Expanded by IL-2 and Inhibited by IL-27/STAT1. ,
Nat Med
 13 , 711 , 2007
Cells
Retinal
Enzymes
Medium
RPMI
Eye
Mouse
Mouse, 2-5 month
Maxeiner Stephan, Dedek Karin, Janssen-Bienhold Ulrike, Ammermuller Josef, Brune Hendrik, Kirsch Taryn, Pieper Mario, Degen Joachim, Kruger Olaf, Willecke Klaus, Weiler Reto.
Deletion of connexin45 in mouse retinal neurons disrupts the rod/cone signaling pathway between AII amacrine and ON cone bipolar cells and leads to impaired visual transmission ,
J Neurosci
 25 , 566-76 , 2005
Cells
Mouse retinal and bipolar
Enzymes
Papain : 20 u/ml
Deoxyribonuclease I : 200 u/ml
Medium
HBSS
Eye
Rat

Rat, SD, 1-2 day

Lian, W. , Hu, X. , Zhang, J. , Wu, Y. , Zhao, N. , Ma, H. , He, H. and Lu, Q.
Fucoxanthin Protects Retinal Ganglion Cells and Promotes Parkin-mediated Mitophagy against Glutamate Excitotoxicity. ,
Neuroreport
 34 , 385-394 , 2023
Cells
Retinal ganglion
Enzymes
Papain : 0.5%
Medium
MEM
Eye
Rat
Rat, Wister, 3-30 wk
Cameron, M. , Kekesi, O. , Morley, J. , Tapson, J. , Breen, P. , Van Schaik, A. and Buskila, Y.
Calcium Imaging of AM Dyes Following Prolonged Incubation in Acute Neuronal Tissue. ,
PLoS ONE
 11 , e0155468 , 2016
Cells
Retinal
Enzymes
Papain : 30 u/ml
Medium
EBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.