Tissue References Search Tool

Human

Fukushima, K. and Fiocchi, C.

Paradoxical Decrease of Mitochondrial DNA Deletions in Epithelial Cells of Active Ulcerative Colitis Patients. ,
Am J Physiol Gastrointest Liver Physiol
286 , G804-13 , 2004

Cells

Colonic epithelial

Enzymes

Neutral Protease : 0.3%

Deoxyribonuclease I : 0.05%

Medium

RPMI 1640

Epithelial

Human

Widdicombe, J.H. , Coleman, D.L. , Finkbeiner, W.E. and Tuet, I.K.

Electrical Properties of Monolayers Cultured From Cells of Human Tracheal Mucosa ,
J Appl Physiol
58 , 1729 , 1985

Cells

Epithelial

Enzymes

Deoxyribonuclease I : 0.01%

Medium

HEPES with 5.9mM Glucose,5mM DTT

Epithelial

Mouse

Mouse, 8-12 week

Brann, D. , Tsukahara, T. , Weinreb, C. , Lipovsek, M. , Van den Berge, K. , Gong, B. , Chance, R. , Macaulay, I. , Chou, H. , Fletcher, R. , Das, D. , Street, K. , de Bezieux, H. , Choi, Y. , Risso, D. and Dudoit, S.

Non-Neuronal Expression of SARS-CoV-2 Entry Genes in the Olfactory System Suggests Mechanisms Underlying COVID-19-Associated Anosmia. ,
Sci. Adv.
6 , 2020

Cells

Nasal epithelium

Enzymes

Papain : 20 u/ml

Deoxyribonuclease I : 0.005%

Medium

EBSS

Epithelial

Mouse

Sun, F. , Xiao, G. and Qu, Z.

Isolation of Murine Alveolar Type II Epithelial Cells. ,
Bio-protocol
7 , 2017

Cells

Alveolar epithelial

Enzymes

Neutral Protease : 0.1%

Deoxyribonuclease I : 0.01%

Medium

DMEM

Epithelial

Mouse

Mouse, 6-8 week

Wirtz S. , Becker C. , Blumberg R. , Galle P. and Neurath M.

Treatment of T cell-dependent experimental colitis in SCID mice by local administration of an adenovirus expressing IL-18 antisense mRNA ,
J Immunol
168(1) , 411 , 2002

Cells

Lamina propria mononuclear cells

Enzymes

Collagenase Type 2 : 0.015%

Deoxyribonuclease I : 0.01%

Medium

RPMI

Epithelial

Mouse

Mouse, 11 week

Mueller, S. , Clark, J. , Myers, P. and Korach, K.

Mammary Gland Development in Adult Mice Requires Epithelial and Stromal Estrogen Receptor Alpha. ,
Endocrinology
143 , 2357 , 2002

Cells

Epithelial

Enzymes

Collagenase Type 3 : 25 u/ml

Hyaluronidase : 0.1%

Protease XIV : 0.05%

Deoxyribonuclease I : 0.04%

Medium

DMEM/F12

Epithelial

Rat

Rat, embryonic

Shannon JM, Gebb SA. and Nielsen LD

Induction of alveolar type II cell differentiation in embryonic tracheal epithelium in mesenchyme-free culture ,
Development
126 , 1675 , 1999

Cells

Tracheal epithelial

Enzymes

Collagenase Type 4 : 0.05%

Medium

DMEM/F12

Epithelial

Rat

Rat, SD, 200 g

Yassin, R. , Clearfield, H. , Katz, S. and Murthy, S.

Gastrin Induction of mRNA Expression in Rat Colonic Epithelium In Vitro ,
Peptides
12 , 63 , 1991

Cells

Colon

Enzymes

Deoxyribonuclease I : 10 µg/ml

Medium

(see reference)

Miscellaneous

Human

Patino WillmarD, Kang Ju-Gyeong, Matoba Satoaki, Mian OmarY, Gochuico BernadetteR, Hwang PaulM.

Atherosclerotic plaque macrophage transcriptional regulators are expressed in blood and modulated by tristetraprolin ,
Circ Res
98 , 1282-9 , 2006

Cells

Carotid artery plaque macrophage

Enzymes

Collagenase Type 4 : 450 u/ml

Deoxyribonuclease I : 500 u/ml

Soybean Trypsin Inhibitor : 0.1%

Medium

HBSS

Miscellaneous

Mouse

Hameed, M. , Rai, P. , Makris, M. and Weger-Lucarelli, J.

Optimized Protocol for Mouse Footpad Immune Cell Isolation for Single-cell RNA Sequencing and Flow Cytometry. ,
Star Protocols
4 , 102409 , 2023

Cells

Leucocytes

Enzymes

Collagenase Type 1 : 0.25%

Deoxyribonuclease I : 0.002%

Medium

RPMI-1640

Miscellaneous

Mouse

Mouse, embryonic

He, M. , Wu, B. , Ye, W. , Le, D. , Sinclair, A. , Padovano, V. , Chen, Y. , Li, K. , Sit, R. , Tan, M. , Caplan, M. , Neff, N. , Jan, Y. , Darmanis, S. and Jan, L.

Chloride Channels Regulate Differentiation and Barrier Functions of the Mammalian Airway. ,
Elife
9 , 53085 , 2020

Cells

Trachea

Enzymes

Elastase : 0.1%

Neutral Protease : 0.25%

Deoxyribonuclease I : 0.01%

Trypsin : 0.125%

Medium

DMEM/F12

Miscellaneous

Mouse

Mouse, 8-12 week

Atta, H. , Gheinani, A. , Wacker, A. , Heshmati, Y. , Bigger-Allen, A. , Lambrinos, G. , Gao, Y. , Bielenberg, D. and Adam, R.

A Single Cell Dissociation Approach for Molecular Analysis of Urinary Bladder in the Mouse Following Spinal Cord Injury. ,
J Vis Exp
, 2020

Cells

Urinary bladder

Enzymes

Collagenase Type 1 : 50 u/ml

Collagenase Type 3 : 40 u/ml

Papain : 45 u/ml

Deoxyribonuclease I : 4 u/ml

Collagenase Type 6 : 20 u/ml

Medium

Tyrode solution

Miscellaneous

Mouse

Mouse, 10 week

Futami, I. , Ishijima, M. , Kaneko, H. , Tsuji, K. , Ichikawa-Tomikawa, N. , Sadatsuki, R. , Muneta, T. , Arikawa-Hirasawa, E. , Sekiya, I. and Kaneko, K.

Isolation and Characterization of Multipotential Mesenchymal Cells from the Mouse Synovium. ,
PLoS ONE
7 , e45517 , 2012

Cells

Synovial mesenchymal

Enzymes

Collagenase : 0.1%

Deoxyribonuclease I : 0.005%

Medium

DMEM

Miscellaneous

Mouse

Stock, A. , Booth, S. and Cerundolo, V.

Prostaglandin E2 Suppresses the Differentiation of Retinoic Acid-Producing Dendritic Cells in Mice and Humans. ,
J Exp Med
208 , 761 , 2011

Cells

Dentritic

Enzymes

Collagenase Type 2 : 0.1%

Deoxyribonuclease I : 0.001%

Medium

DME

Miscellaneous

Mouse

Shi, C. , Jia, T. , Mendez-Ferrer, S. , Hohl, T. , Serbina, N. , Lipuma, L. , Leiner, I. , Li, M. , Frenette, P. and Pamer, E.

Bone Marrow Mesenchymal Stem and Progenitor Cells Induce Monocyte Emigration in Response to Circulating Toll-Like Receptor Ligands. ,
Immunity
34 , 590 , 2011

Cells

Spleen, bone marrow endothelial

Enzymes

Collagenase Type 4 : 0.3-1.0%

Deoxyribonuclease I : 20 u/ml

Medium

PBS

Miscellaneous

Mouse

Mouse, 6-10 week

Ji, H. , Rintelen, F. , Waltzinger, C. , Meier, D, Bilancio, A. , Pearce, W. , Hirsch, E. , Wymann, M. , Ruckle, T. , Camps, M. , Vanhaesebroeck, B. , Okkenhaug, K. and Rommel, C.

Inactivation of PI3Kgamma and PI3Kdelta Distorts T-cell Development and Causes Multiple Organ Inflammation. ,
Blood
110 , 2940 , 2007

Cells

Salivary gland and stomach

Enzymes

Collagenase Type 4 : 0.8%

Deoxyribonuclease I : 1.0%

Medium

RPMI-1640

Miscellaneous

Mouse

Mouse, 25-30 g

Minamoto Kanji, Pinsky DavidJ.