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Tissue References Search Tool

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Liver
Chicken
Chicken, Leghorn, white, 10-15 day
Tarlow, D. , Watkins, P. , Reed, R. , Miller, R. , Zwergel, E. and Lane, M.
Lipogenesis and the Synthesis and Secretion of Very Low Density Lipoprotein by Avian Liver Cells in Nonproliferating Monolayer Culture ,
J Cell Biol
 73 , 332 , 1974
Cells
Hepatocytes
Enzymes
Deoxyribonuclease I : 0.00125%
Medium
PBS
Liver
Human

Human

Kucykowicz, S. , Amin, O. , Burton, A. , Swadling, L. , Schmidt, N. , Zakeri, N. , Davies, J. , Aidoo-Micah, G. , Stegmann, K. , Easom, N. , Jeffery-Smith, A. , Maini, M. and Pallett, L.
Isolation of Human Intrahepatic Leukocytes for Phenotypic and Functional Characterization by Flow Cytometry. ,
Star Protocols
 3 , 101356 , 2022
Cells
Intrahepatic leukocytes
Enzymes
Medium
RPMI-1640
Liver
Human
Human, fetal
Vatakis, D. , Bristol, G. , Kim, S. , Levin, B. , Liu, W. , Radu, C. , Kitchen, S. and Zack, J.
Using the BLT Humanized Mouse as a Stem Cell Based Gene Therapy Tumor Model. ,
J Vis Exp
 70 , e4181 , 2012
Cells
Liver hematopoietic
Enzymes
Medium
RPMI
Liver
Mouse
Mouse
Perepelyuk, M. , Terajima, M. , Wang, A. , Georges, P. , Janmey, P. , Yamauchi, M. and Wells, R.
Hepatic Stellate Cells and Portal Fibroblasts are the Major Cellular Sources of Collagens and Lysyl Oxidases in Normal Liver and Early After Injury. ,
Am J Physiol Gastrointest Liver Physiol
 304 , G605 , 2013
Cells
Hepatocytes and sinusoidal endothelial
Enzymes
Medium
HBSS
Liver
Mouse
Mouse
Rountree, C. , Ding, W. , Dang, H. , Vankirk, C. and Crooks, G.
Isolation of CD133+ Liver Stem Cells for Clonal Expansion. ,
J Vis Exp
 56 , e3183 , 2011
Cells
CD133+ stem
Enzymes
Pronase : 0.05%
Medium
DMEM/F12
Liver
Mouse
Mouse
Li Wen-Lin, Su Juan, Yao Yu-Cheng, Tao Xin-Rong, Yan Yong-Bi, Yu Hong-Yu, Wang Xin-Min, Li Jian-Xiu, Yang Yong-Ji, Lau Joseph T Y, Hu Yi-Ping.
Isolation and characterization of bipotent liver progenitor cells from adult mouse ,
Stem Cells
 24 , 322-32 , 2006
Cells
Liver epithelial progenitor cells
Enzymes
Medium
DMEM
Liver
Porcine
Porcine, mini-pig, 13 kg
Meng, F. , Chen, Z. , Han, M. , Hu, X. , He, X. , Liu, Y. , He, W. , Huang, W. , Guo, H. and Zhou, P.
Porcine Hepatocyte Isolation and Reversible Immortalization Mediated by Retroviral Transfer and Site-Specific Recombination. ,
World J Gastroenterol
 16 , 1660 , 2010
Cells
Hepatocytes
Enzymes
Deoxyribonuclease I : see reference
Medium
Williams E
Liver
Rat
Rat, SD, 500-700g
Perepelyuk, M. , Terajima, M. , Wang, A. , Georges, P. , Janmey, P. , Yamauchi, M. and Wells, R.
Hepatic Stellate Cells and Portal Fibroblasts are the Major Cellular Sources of Collagens and Lysyl Oxidases in Normal Liver and Early After Injury. ,
Am J Physiol Gastrointest Liver Physiol
 304 , G605 , 2013
Cells
Hepatocytes and sinusoidal endothelial
Enzymes
Medium
HBSS
Liver
Rat
Rat, adult
Jensen C. , Jauho E. , Santoni-Rugiu E. , Holmskov U. , Teisner B. , Tygstrup N. and Bisgaard H.
Transit-amplifying Ductular (oval) Cells and Their Hepatocytic Progeny are Characterized by a Novel and Distinctive Expression of Delta-like Protein/preadipocyte Factor 1/fetal Antigen 1 ,
Am J Pathol
 164(4) , 1347 , 2004
Cells
Hepatocytes
Enzymes
Pronase : 1%
Medium
Williams E
Miscellaneous
Human
Human
Patino WillmarD, Kang Ju-Gyeong, Matoba Satoaki, Mian OmarY, Gochuico BernadetteR, Hwang PaulM.
Atherosclerotic plaque macrophage transcriptional regulators are expressed in blood and modulated by tristetraprolin ,
Circ Res
 98 , 1282-9 , 2006
Cells
Carotid artery plaque macrophage
Enzymes
Medium
HBSS
Miscellaneous
Mouse

Mouse

Hameed, M. , Rai, P. , Makris, M. and Weger-Lucarelli, J.
Optimized Protocol for Mouse Footpad Immune Cell Isolation for Single-cell RNA Sequencing and Flow Cytometry. ,
Star Protocols
 4 , 102409 , 2023
Cells
Leucocytes
Enzymes
Medium
RPMI-1640
Miscellaneous
Mouse
Mouse, embryonic
He, M. , Wu, B. , Ye, W. , Le, D. , Sinclair, A. , Padovano, V. , Chen, Y. , Li, K. , Sit, R. , Tan, M. , Caplan, M. , Neff, N. , Jan, Y. , Darmanis, S. and Jan, L.
Chloride Channels Regulate Differentiation and Barrier Functions of the Mammalian Airway. ,
Elife
 9 , 53085 , 2020
Cells
Trachea
Enzymes
Elastase : 0.1%
Trypsin : 0.125%
Medium
DMEM/F12
Miscellaneous
Mouse
Mouse, 8-12 week
Atta, H. , Gheinani, A. , Wacker, A. , Heshmati, Y. , Bigger-Allen, A. , Lambrinos, G. , Gao, Y. , Bielenberg, D. and Adam, R.
A Single Cell Dissociation Approach for Molecular Analysis of Urinary Bladder in the Mouse Following Spinal Cord Injury. ,
J Vis Exp
 , 2020
Cells
Urinary bladder
Enzymes
Collagenase Type 1 : 50 u/ml
Collagenase Type 3 : 40 u/ml
Papain : 45 u/ml
Collagenase Type 6 : 20 u/ml
Medium
Tyrode solution
Miscellaneous
Mouse
Mouse, 10 week
Futami, I. , Ishijima, M. , Kaneko, H. , Tsuji, K. , Ichikawa-Tomikawa, N. , Sadatsuki, R. , Muneta, T. , Arikawa-Hirasawa, E. , Sekiya, I. and Kaneko, K.
Isolation and Characterization of Multipotential Mesenchymal Cells from the Mouse Synovium. ,
PLoS ONE
 7 , e45517 , 2012
Cells
Synovial mesenchymal
Enzymes
Medium
DMEM
Miscellaneous
Mouse
Mouse
Stock, A. , Booth, S. and Cerundolo, V.
Prostaglandin E2 Suppresses the Differentiation of Retinoic Acid-Producing Dendritic Cells in Mice and Humans. ,
J Exp Med
 208 , 761 , 2011
Cells
Dentritic
Enzymes
Medium
DME
Miscellaneous
Mouse
Mouse
Shi, C. , Jia, T. , Mendez-Ferrer, S. , Hohl, T. , Serbina, N. , Lipuma, L. , Leiner, I. , Li, M. , Frenette, P. and Pamer, E.
Bone Marrow Mesenchymal Stem and Progenitor Cells Induce Monocyte Emigration in Response to Circulating Toll-Like Receptor Ligands. ,
Immunity
 34 , 590 , 2011
Cells
Spleen, bone marrow endothelial
Enzymes
Medium
PBS
Miscellaneous
Mouse
Mouse, 6-10 week
Ji, H. , Rintelen, F. , Waltzinger, C. , Meier, D, Bilancio, A. , Pearce, W. , Hirsch, E. , Wymann, M. , Ruckle, T. , Camps, M. , Vanhaesebroeck, B. , Okkenhaug, K. and Rommel, C.
Inactivation of PI3Kgamma and PI3Kdelta Distorts T-cell Development and Causes Multiple Organ Inflammation. ,
Blood
 110 , 2940 , 2007
Cells
Salivary gland and stomach
Enzymes
Medium
RPMI-1640
Miscellaneous
Mouse
Mouse, 25-30 g
Minamoto Kanji, Pinsky DavidJ.
Recipient iNOS but not eNOS deficiency reduces luminal narrowing in tracheal allografts ,
J Exp Med
 196 , 1321-33 , 2002
Cells
Tracheal inflammatory cells
Enzymes
Medium
RPMI 1640

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.