For our international customers, please be advised that orders cannot be placed through our website by customers in countries with International Distributor representation.

Quick Order

Tissue References Search Tool

Search
Heart
Chicken
Chicken, embryonic
Blech-Hermoni, Y. and Ladd, A.
Identification of Transcripts Regulated by CUG-BP, Elav-like Family Member 1 (CELF1) in Primary Embryonic Cardiomyocytes by RNA-seq. ,
Genom Data
 6 , 74-76 , 2015
Cells
Cardiomyocytes
Enzymes
Medium
HBSS
Heart
Mouse
Mouse
Hu, D. , Yin, C. , Mohanta, S. , Weber, C. and Habenicht, A.
Prepartaion of Single Cell Suspensions from Mouse Aorta ,
Bio Protoc
 6 , 11 , 2016
Cells
Aortic
Enzymes
Collagenase Type 1 : 400 u/ml
Collagenase : 120 u/ml
Hyaluronidase : 60 u/ml
Medium
DPBS
Heart
Mouse
Mouse, adult
Santos Nascimento, D, Mosqueira, D, Sousa, L, Teixeira, M, Filipe, M, Resende, T, Araujo, A, Valente, M, Almeida, J, Martins, J, Santos, J, Barcia, R, Cruz, P, Cruz, H. and Pinto-d-O, P
Human Umbilical Cord Tissue-Derived Mesenchymal Stromal Cells Attenuate Remodeling after Myocardial Infarction by Proangiogenic, Antiapoptotic, and Endogenous Cell-Activation Mechanisms. ,
Stem Cell Res Ther
 5 , 5 , 2014
Cells
Cardiac Progenitor
Enzymes
Medium
MEM
Heart
Mouse
Mouse
Touchberry, C. , Green, T. , Tchikrizov, V. , Mannix, J. , Mao, T. , Carney, B. , Girgis, M. , Vincent, R. , Wetmore, L. , Dawn, B. , Bonewald, L. , Stubbs, J. and Wacker, M.
FGF23 is a Novel Regulator of Intracellular Calcium and Cardiac Contractility in Addition to Cardiac Hypertrophy. ,
Am J Physiol Endocrinol Metab
 304 , E863 , 2013
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 360 u/ml
Papain : 0.4 u/ml
Medium
L-15
Heart
Mouse
Mouse, adult, 9-13 week
Kabaeva, Z. , Zhao, M. and Michele, D.
Blebbistatin Extends Culture Life of Adult Mouse Cardiac Myocytes and Allows Efficient and Stable Transgene Expression. ,
Am J Physiol Heart Circ Physiol
 294 , H1667-74 , 2008
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 620 u/ml
Protease XIV : 0.104 u/ml
Medium
Myocyte buffer see reference
Heart
Mouse
Mouse, 6-8 week
Qian, Q. , Hunter, L. , Li, M. , Marin-Padilla, M. , Prakash, Y. , Somlo, S. , Harris, P. , Torres, V. and Sieck, G.
Pkd2 Haploinsufficiency Alters Intracellular Calcium Regulation in Vascular Smooth Muscle Cells ,
Hum Mol Genet
 12(15) , 1875 , 2003
Cells
Vascular smooth muscle
Enzymes
Papain : 10 u/ml
Elastase : .005%
Collagenase : 0.05%
Deoxyribonuclease I : 1000 u/ml
PDS kit : with modifications
Medium
EBSS
Heart
Mouse
Mouse, 1-6 day
Watzka SB, Lucien J, Shimada M, Edwards V, Yeger H, Hannigan G. and Coles JG.
Selection of viable cardiomyocytes for cell transplantation using three-dimensional tissue culture ,
Transplantation
 70 , 1310 , 2000
Cells
Cardiomyocytes
Enzymes
Medium
PBS
Heart
Mouse
Mouse, 6-9 or 21-41 day
Valenzuela, D. , Han, X. , Mende, U. , Fankhauser, C. , Mashimo, H. , Huang, P. , Pfeffer, J. , Neer, E. and Fishman, M.
G alpha(o) is Necessary for Muscarinic Regulation of Ca2+ Channels in Mouse Heart ,
Proc Natl Acad Sci U S A
 94(5) , 1727 , 1997
Cells
Ventricular and atrial myocytes
Enzymes
Collagenase Type 2 : 95 u/ml
Hyaluronidase : 172.5 u/ml
Trypsin : 0.002%
Medium
DMEM/Tyrodes
Heart
Rat
Rat, SD, 175-200g
Tardif Annie, Julien Nathalie, Chiasson Jean-Louis, Coderre Lise.
Stimulation of glucose uptake by chronic vanadate pretreatment in cardiomyocytes requires PI 3-kinase and p38 MAPK activation ,
Am J Physiol/Endo
 284 , E1055-64 , 2003
Cells
Cardiomyocytes
Enzymes
Medium
Krebs-Ringer
Skin
Human
Human
Botting, Ra. , Bertram, K. , Baharlou, H. , Sandgren, K. , Fletcher, J. , Rhodes, J. , Rana, H. , Plasto, T. , Wang, X. , Lim, J. , Barnouti, L. , Kohout, M. , Papadopoulos, T. , Merten, S. , Olbourne, N. and Cunningham, A.
Phenotypic and Functional Consequences of Different Isolation Protocols on Skin Mononuclear Phagocytes. ,
J Leukoc Biol
 101 , 1393-1403 , 2017
Cells
Mononuclear phagocytes
Enzymes
Neutral Protease : 1 u/ml
Collagenase : 200 u/ml
Trypsin : 0.5%
Medium
RPMI-1640
Skin
Human
Human
Cheuk, S. , Wiken, M. , Blomqvist, L. , Nylen, S. , Talme, T. and Stahle, M.and Eidsmo, L.
Epidermal Th22 and Tc17 Cells Form a Localized Disease Memory in Clinically Healed Psoriasis. ,
J Immunol
 192 , 3111 , 2014
Cells
Skin
Enzymes
Medium
RPMI 1640
Skin
Human
Human
Cerny, D. , Haniffa, M. , Shin, A. , Bigliardi, P. , Tan, B. , Lee, B. , Poidinger, M. , Tan, E. , Ginhoux, F. and Fink, K.
Selective Susceptibility of Human Skin Antigen Presenting Cells to Productive Dengue Virus Infection. ,
PLoS Pathog
 10 , e1004548 , 2014
Cells
Skin
Enzymes
Medium
RPMI
Skin
Human
Human, adult
Alitalo K, Kuismanen E, Myllyla R, Kiistala U, Asko-Seljavaara S, Vaheri A.
Extracellular matrix proteins of human epidermal keratinocytes and feeder 3T3 cells ,
J Cell Biol
 94 , 497-505 , 1982
Cells
Epidermal keratinocytes
Enzymes
Trypsin : 0.25%
Collagenase : 0.2%
Medium
Eagle's MEM
Skin
Mouse
Mouse
Spidale, N. , Malhotra, N. , Frascoli, M. , Sylvia, K. , Miu, B. , Freeman, C. , Stadinski, B. , Huseby, E. and Kang, J.
Neonatal-Derived IL-17 Producing Dermal gd T Cells are Required to Prevent Spontaneous Atopic Dermatitis ,
Elife
 9 , 5118 , 2020
Cells
Keratinocytes
Enzymes
Medium
DMEM
Skin
Mouse
Mouse
Cerny, D. , Haniffa, M. , Shin, A. , Bigliardi, P. , Tan, B. , Lee, B. , Poidinger, M. , Tan, E. , Ginhoux, F. and Fink, K.
Selective Susceptibility of Human Skin Antigen Presenting Cells to Productive Dengue Virus Infection. ,
PLoS Pathog
 10 , e1004548 , 2014
Cells
Skin
Enzymes
Medium
RPMI
Skin
Mouse
Mouse
Eidsmo, L. , Allan, R. , Caminschi, I. , Van Rooijen, N. , Heath, W. and Carbone, F.
Differential Migration of Epidermal and Dermal Dendritic Cells During Skin Infection. ,
J Immunol
 182 , 3165 , 2009
Cells
Dermal
Enzymes
Medium
HBSS
Skin
Mouse
Mouse, neonatal
Crigler Lauren, Kazhanie Amita, Yoon Tae-Jin, Zakhari Julia, Anders Joanna, Taylor Barbara, Virador VictoriaM.
Isolation of a mesenchymal cell population from murine dermis that contains progenitors of multiple cell lineages ,
FASEB J
 21 , 2050-63 , 2007
Cells
Dermal
Enzymes
Collagenase : 0.35%
Deoxyribonuclease I : see reference
Medium
PBS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.