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Tissue References Search Tool

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Organoids
Human

Human

Gamero-Estevez, E. , Hensel, I. , Steinhauer, M. , Müllertz, O. , Savochkina, E. , Sektioglu, I. , Stoll, B. , Derakhshani, S. , Devriese, S. , Kim, K. and Resnik-Docampo, M.
Protocol for Immune Cell Isolation, Organoid Generation, and Co-culture Establishment from Cryopreserved Whole Human Intestine. ,
Bio Protoc
 15 , e5157 , 2025
Cells
Intestinal organoids
Enzymes
Medium
RPMI
Organoids
Human

Human

Geest, A. , Jakobs, C. , Ljubikj, T. , Huffels, C. , Luna, M. , Sá, R. , Adolfs, Y. , Wit, M. , Rutten, D. , Kaal, M. , Zwartkruis, M. , Carcolé, M. , Groen, E. , Hol, E. , Basak, O. , Isaacs, A. , Westeneng, H. , Berg, L. , Veldink, J. , Schlegel, D. and Pasterkamp, R.
Molecular Pathology, Developmental Changes, and Synaptic Dysfunction in (pre-) Symptomatic Human C9ORF72-ALS/FTD Cerebral Organoids. , Acta Neuropathol Commun Vol. 12 , 152 , 2024
Cells
Cerebral organoids
Enzymes
Papain : 20 u/ml
Medium
DMEM/F12
Organoids
Human

Human

Lisowski, P. , Lickfett, S. , Rybak-Wolf, A. , Menacho, C. and Le, S.
Mutant Huntingtin Impairs Neurodevelopment in Human Brain Organoids through CHCHD2-mediated Neurometabolic Failure. ,
Nat Commun
 15 , 7027 , 2024
Cells
Brain organoids
Enzymes
Papain : 20 u/ml
Medium
EBSS
Organoids
Human

Human

Marinucci, M. , Ercan, C. , Taha-Mehlitz, S. , Fourie, L. , Panebianco, F. , Bianco, G. , Gallon, J. , Staubli, S. , Soysal, S. , Zettl, A. , Rauthe, S. , Vosbeck, J. , Droeser, R. , Bolli, M. , Peterli, R. , Flüe, M. , Ng, C. , Kollmar, O. , Coto-Llerena, M. and Piscuoglio, S.
Standardizing Patient-Derived Organoid Generation Workflow to Avoid Microbial Contamination From Colorectal Cancer Tissues. , Front Oncol Vol. 11 , 781833 , 2021
Cells
Colorectal cancer organoids
Enzymes
Medium
DMEM/F-12
Organoids
Human

Human

Schreurs, R. , Baumdick, M. , Drewniak, A. and Bunders, M.
Co-culture of Human Intestinal Organoids and Lamina Propria-derived CD4 T Cells. , STAR Protoc Vol. 2 , 100519 , 2021
Cells
Lamina propria lymphocytes
Enzymes
Medium
DMEM/F-12
Organoids
Human

Human, embryonic

Nikolić, M. , Caritg, O. , Jeng, Q. , Johnson, J. , Sun, D. , Howell, K. , Brady, J. , Laresgoiti, U. , Allen, G. , Butler, R. , Zilbauer, M. , Giangreco, A. and Rawlins, E.
Human Embryonic Lung Epithelial Tips are Multipotent Progenitors that can be Expanded In vitro as Long-term Self-renewing Organoids. ,
Elife
 6 , 2017
Cells
Lung organoids
Enzymes
Medium
DMEM/F12
Organoids
Mouse

Mouse

Fujimura, T. , Enomoto, Y. , Katsura, H. , Ogawa, T. , Baba, S. , Ogata, A. , Yamaoka, A. , Shiroguchi, K. and Morimoto, M.
Identifying a Lung Stem Cell Subpopulation by Combining Single-Cell Morphometrics, Organoid Culture, and Transcriptomics. ,
Stem Cells
 41 , 809-820 , 2023
Cells
Lung
Enzymes
Collagenase Type 1 : 450 u/ml
Elastase : 1 u/ml
Neutral Protease : 5 u/ml
Medium
DMEM/F12
Organoids
Mouse

Mouse, embryonic

Nikolić, M. , Caritg, O. , Jeng, Q. , Johnson, J. , Sun, D. , Howell, K. , Brady, J. , Laresgoiti, U. , Allen, G. , Butler, R. , Zilbauer, M. , Giangreco, A. and Rawlins, E.
Human Embryonic Lung Epithelial Tips are Multipotent Progenitors that can be Expanded In vitro as Long-term Self-renewing Organoids. ,
Elife
 6 , 2017
Cells
Lung organoids
Enzymes
Medium
DMEM/F12
Organoids
Mouse

Mouse

O'Rourke, K. , Ackerman, S. , Dow, L. and Lowe, S.
Isolation, Culture, and Maintenance of Mouse Intestinal Stem Cells. ,
Bio Protoc
 6 , 2016
Cells
Intestinal organoids
Enzymes
Medium
Basal Medium
Reproductive
Chicken

Chicken, male, 1 day

Huang, H. , Wang, M. , Hou, L. , Lin, X. , Pan, S. , Zheng, P. and Zhao, Q.
A Potential Mechanism Associated with Lead-induced Spermatogonia and Leydig Cell Toxicity and Mitigative Effect of Selenium in Chicken. , Ecotoxicol Environ Saf Vol. 209 , 111671 , 2021
Enzymes
Collagenase : 0.1%
Hyaluronidase : 0.1%
Trypsin : 0.1%
Medium
DMEM
Reproductive
Human
Human
Evans, J. and Salamonsen, L.
Decidualized Human Endometrial Stromal Cells are Sensors of Hormone Withdrawal in the Menstrual Inflammatory Cascade. ,
Biol Reprod
 90 , 14 , 2014
Cells
Endometrial stromal
Enzymes
Medium
DMEM/F-12
Reproductive
Human
Human
Patel, J. , Seppanen, E. , Chong, M. , Yeo, J. , Teo, E. , Chan, J. , Fisk, N. and Khosrotehrani, K.
Prospective Surface Marker-Based Isolation and Expansion of Fetal Endothelial Colony-Forming Cells from Human Term Placenta. ,
Stem Cells Transl Med
 2 , 839-47 , 2013
Cells
Endothelial colony forming
Enzymes
Medium
HBSS
Reproductive
Human
Human
Shalini, V. , Pratheep, S. , Muhammad, A. , Sharmili, V. , Elizabeth, G. and Rajesh, R.
Generation and Characterisation of Human Mesenchymal Stem Cells Derived From Umbilical Cord and Placenta ,
Regenerative Research
 1 , 48 , 2012
Cells
Mesenchymal stem
Enzymes
Medium
DMEM/F12
Reproductive
Human
Human, female
Lockwood, C. , Arcuri, F. , Toti, P. , Felice, C. , Krikun, G. , Guller, S. , Buchwalder, L. and Schatz, F.
Tumor Necrosis Factor-Alpha and Interleukin-1 Beta Regulate Interleukin-8 Expression in Third Trimester Decidual Cells: Implications for the Genesis of Chorioamnionitis. ,
Am J Pathol
 169 , 1294-302 , 2006
Cells
Decidual
Enzymes
Collagenase : 0.25%
Deoxyribonuclease I : 6.25 u/ml
Medium
DMEM/F12
Reproductive
Human
Human
Spessotto, P. , Bulla, R. , Danussi, C. , Radillo, O. , Cervi, M. , Monami, G. , Bossi, F. , Tedesco, F. , Doliana, R. and Colombatti, A.
EMILIN1 Represents a Major Stromal Element Determining Human Trophoblast Invasion of the Uterine Wall. ,
J Cell Sci
 119 , 4574 , 2006
Cells
Extravillanous trophoblasts and decidual stromal
Enzymes
Trypsin : 0.1-0.25%
Deoxyribonuclease I : 0.02-0.05%
Medium
HBSS RPMI
Reproductive
Human
Human
Wang Xin, Athayde Neil, Trudinger Brian.
Microvascular endothelial cell activation is present in the umbilical placental microcirculation in fetal placental vascular disease ,
Am J Obstet Gynecol
 190 , 596-601 , 2004
Cells
Endothelial placental
Enzymes
Medium
DMEM
Reproductive
Human
Human, female, 34-51 yr
Chan Rachel W S, Schwab Kjiana E, Gargett Caroline E.
Clonogenicity of human endometrial epithelial and stromal cells ,
Biol Reprod
 70 , 1738-50 , 2004
Cells
Endometrial epithelial and stromal cells
Enzymes
Medium
DMEM/F-12
Reproductive
Human
Human
Sun Kang, Myatt Leslie.
Enhancement of glucocorticoid-induced 11beta-hydroxysteroid dehydrogenase type 1 expression by proinflammatory cytokines in cultured human amnion fibroblasts ,
Endocrinology
 144 , 5568-77 , 2003
Cells
Amnion epithelial and fibroblast
Enzymes
Trypsin : 0.125%
Collagenase : 0.1%
Medium
PBS
Reproductive
Human
Human, female
Witz Craig A, Allsup Karen T, Montoya-Rodriguez Iris A, Vaughn Shelby L, Centonze Victoria E, Schenken Robert S.
Culture of menstrual endometrium with peritoneal explants and mesothelial monolayers confirms attachment to intact mesothelial cells ,
Hum Reprod
 17 , 2832-8 , 2002
Cells
Mesothelial
Enzymes
Medium
Eagle's MEM
Reproductive
Human
Human, female, 20-40 year
Gargett CE, Bucak K, Rogers PA.
Isolation, characterization and long-term culture of human myometrial microvascular endothelial cells ,
Hum Reprod
 15 , 293-301 , 2000
Cells
Microvascular endothelial cells
Enzymes
Medium
PBS
Reproductive
Human
Human
Friden BE, Runesson E, Hahlin M, Brannstrom M.
Evidence for nitric oxide acting as a luteolytic factor in the human corpus luteum ,
Mol Hum Reprod
 6 , 397-403 , 2000
Cells
Corpus luteum cells
Enzymes
Medium
PBS
Reproductive
Human
Human
Runesson E, Ivarsson K, Janson P, Brannstrom M.
Gonadotropin- and cytokine-regulated expression of the chemokine interleukin 8 in the human preovulatory follicle of the menstrual cycle ,
J Clin Endocrinol Metab
 85 , 4387-95 , 2000
Cells
Theca cells
Enzymes
Medium
PBS
Reproductive
Human
Human, female
Zhang J, Lathbury LJ, Salamonsen LA.
Expression of the chemokine eotaxin and its receptor, CCR3, in human endometrium ,
Biol Reprod
 62 , 404-11 , 2000
Cells
Endometrial epithelial cells
Enzymes
Medium
DMEM/F-12
Reproductive
Human
Human, females, 25-45 yr
Friden BE, Hagstrom H, Lindblom B, Sjoblom P, Wallin A, Brannstrom M, Hahlin M.
Cell characteristics and function of two enriched fraction of human luteal cells prolonged culture ,
Mol Hum Reprod
 5 , 714-9 , 1999
Cells
Luteal cells
Enzymes
Medium
PBS
Reproductive
Human
Human
Bradbury. , Sunn, K. , Crossley, M. , Bai, M. , Brown, E. , Delbridge, D. and Conigrave, A.
Expression of the Parathyroid Ca 2+ - Sensing Receptor in Cytotrophoblasts From Human Term Placenta ,
J Endocrinol
 156 , 425-430 , 1998
Cells
Placental
Enzymes
Medium
PSS
Reproductive
Human
Human
Zhang J, Nie G, Jian W, Woolley DE, Salamonsen LA.
Mast cell regulation of human endometrial matrix metalloproteinases: A mechanism underlying menstruation ,
Biol Reprod
 59 , 693-703 , 1998
Cells
Stromal endometrial
Enzymes
Medium
DMEM/Ham's F12
Reproductive
Human
Human
Egan, D. , Grzegorczyk, V. , Tricarico, K. , Rueter, A.H. , Olleman, W. and Marcotte, P.
Human Placental Chorionic Renin: Production, Purification and Characterization ,
Biochim Biophys Acta
 965 , 68 , 1988
Cells
Chorionic, placental
Enzymes
Medium
HBSS
Reproductive
Insect
Drosophila, embryonic
Salmand, P. Iche-Torres, M. and Perrin, L.
Tissue-Specific Cell Sorting from Drosophila Embryos: Application to Gene Expression Analysis. , Fly (Austin) Vol. 5 , 261
Cells
Cardiac differentiating
Enzymes
Collagenase Type 1 : 20 u/ml
Trypsin : 0.25%
Medium
Schneider
Reproductive
Monkey
Rhesus macaque
Xu, J, Lawson, MS, Yeoman, RR, Pau, KY, Barrett, SL, Zelinski, MB. and Stouffer, RL
Secondary Follicle Growth and Oocyte Maturation During Encapsulated Three-Dimensional Culture in Rhesus Monkeys: Effects of Gonadotrophins, Oxygen and Fetuin. ,
Hum Reprod
 26 , 1061-72 , 2011
Cells
Follicle
Enzymes
Medium
HEPES
Reproductive
Mouse
Mouse, adult
Lee, A, Rusch, J. , Lima, A. , Usmani, A. , Huang, N. , Lepamets, M. , Vigh-Conrad, K. , Worthington, R. and et al.
Rare Mutations in the Complement Regulatory Gene CSMD1 are Associated with Male and Female Infertility. ,
Nat Commun
 10 , 4626 , 2019
Cells
Testes
Enzymes
Medium
MEM
Reproductive
Mouse
Mouse, 1-7 day
Kang, H. , Chen, L. , Lichti-Kaiser, K. , Liao, G. , Gerrish, K. , Bortner, C. , Yao, Hu. , Eddy, E. and Jetten, A.
Transcription Factor GLIS3: A New and Critical Regulator of Postnatal Stages of Mouse Spermatogenesis. ,
Stem Cells
 34 , 2772-2783 , 2016
Cells
Testicular
Enzymes
Medium
HBSS
Reproductive
Mouse
Mouse, adult
Martin, L. and Seandel, M.
Serial Enrichment of Spermatogonial Stem and Progenitor Cells (SSCs) in Culture for Derivation of Long-Term Adult Mouse SSC Lines. ,
J Vis Exp
 72 , e50017 , 2013
Cells
Spermatogonial stem
Enzymes
Trypsin : 0.05%
Medium
DMEM
Reproductive
Mouse
Mouse, male
Getun, I. , Torres, B. and Bois, P.
Flow Cytometry Purification of Mouse Meiotic Cells. ,
J Vis Exp
 50 , 2602 , 2011
Cells
Testis, meiotic
Enzymes
Collagenase Type 1 : 120 u/ml
Trypsin : 0.1%
Medium
Gey's BSS
Reproductive
Mouse
Mouse, 6 day
Nalbandian Angele, Dettin Luis, Dym Martin, Ravindranath Neelakanta.
Expression of vascular endothelial growth factor receptors during male germ cell differentiation in the mouse ,
Biol Reprod
 69 , 985-94 , 2003
Cells
Sertoli
Enzymes
Medium
DMEM
Reproductive
Mouse
Mouse, 6-12 day
Eppig JJ, O'Brien MJ.
Development in vitro of mouse oocytes from primordial follicles ,
Biol Reprod
 54 , 197-207 , 1996
Cells
Oocyte-granulosa
Enzymes
Medium
Waymouth
Reproductive
Mouse
Mouse, male, 10-13 wk
Stalvcey, J. and Payne, A.
Luteinizing Hormone Receptors and Testosterone Production in Whole Testes and Purified Leydig Cells from the Mouse: Differences among Inbred Strains ,
Endocrinology
 112 , 1696 , 1983
Cells
Leydig
Testis
Enzymes
Medium
Medium 199 w/ BSA

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.