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Tissue References Search Tool

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Liver
Rat
Rat, CD strain, albino, male, 140 -180 g
Braun, L. , Mead, J. , Panzica, M. , Mikumo, R. , Bell, G. and Fausto, N.
Transforming Growth Factor B mRNA Increases During Liver Regeneration:A Possible Paracrine Mechanism Of Growth Regulation ,
Proc Natl Acad Sci U S A
 85 , 1539 , 1988
Cells
Hepatocytes and Nonparenchymal
Enzymes
Pronase : 0.1%
Medium
HEPES buffer with calcium
Liver
Rat
Rat, Wistar, male, 3 month old
Nagelkenke, J. , Barto, K. and VanBerkel, T.
In vivo and in vitro uptake and degradation of acetylated low density lipoprotein by rat liver endothelial, Kupffer, and parenchymal cells ,
J Biol Chem
 258 , 12221 , 1983
Cells
Endothelial
Kupffer
Parenchymal
Enzymes
Collagenase : 0.05%
Pronase : 0.25%
Medium
HBSS
Lung
Guinea-Pig
Guinea-pig, male, 250-300 g
Sikpi, M. , Nair, C. , Johns, A. and Das, S.
Metabolic and Ultrastructural Characterizationof Guinea-Pig Alveolar Type II Cells Isolated by Centrifugal Elutriation ,
Biochim Biophys Acta
 877 , 20 , 1986
Cells
Alveolar type II
Enzymes
Elastase : 40 u/ml
Medium
PBS
(see reference)
Lung
Human
Human
Bahmed, K. , Boukhenouna, S. , Karim, L. , Andrews, T. , Lin, J. , Powers, R. , Wilson, M. , Lin, C. , Messier, E. , Reisdorph, N. , Powell, R. , Tang, H. , Mason, R. , Criner, G. and Kosmider, B.
The Effect of Cysteine Oxidation on DJ-1 Cytoprotective Function in Human Alveolar Type II Cells. ,
Cell Death Dis
 10 , 638 , 2019
Cells
Aveolar
Enzymes
Elastase : 12.9 u/ml
Medium
PBS
Lung
Human
Human
Quatromoni, J. , Singhal, S. , Bhojnagarwala, P. , Hancock, W. , Albelda, S. and Eruslanov, E.
An Optimized Dissagregation Method for Human Lung Tumors That Perserves the Phenotype and Function of the Immune Cells ,
J Leukoc Biol
 97 , 201 , 2015
Cells
Lung tumor
Enzymes
Collagenase Type 1 : 45-60 u/ml
Collagenase Type 2 : 15-20 u/ml
Collagenase Type 4 : 45-60 u/ml
Elastase : 0.002%
Medium
DMEM/F12
Lung
Human
Human
Barkauskas, C. , Cronce, M. , Rackley, C. , Bowie, E. , Keene, D. , Stripp, B. , Randell, S. , Noble, P. and Hogan, B.
Type 2 Alveolar Cells are Stem Cells in Adult Lung ,
J Clin Invest
 123 , 3025 , 2013
Cells
Alveolar epithelial
Enzymes
Neutral Protease : 2 u/ml
Trypsin : 0.25%
Elastase : 10 u/ml
Medium
Bronchial Epithelial Growth Medium
Lung
Mouse

Mouse

Comeau, K. , Caillon, A. , Paradis, P. and Schiffrin, E.
Determination of Interleukin-17A and Interferon-γ Production in γδ, CD4, and CD8 T Cells Isolated from Murine Lymphoid Organs, Perivascular Adipose Tissue, Kidney, and Lung. ,
Bio Protoc
 13 , e4679 , 2023
Cells
T cells
Enzymes
Medium
RPMI
Lung
Mouse
Mouse
Mohapatra, A. , Van Dyken, S. , Schneider, C. , Nussbaum, J. , Liang, H. and Locksley, R.
Group 2 Innate Lymphoid Cells Utilize the IRF4-IL-9 Module to Coordinate Epithelial Cell Maintenance of Lung Homeostasis. ,
Mucosal Immunol
 , 2015
Cells
Alveolar
Enzymes
Collagenase Type 1 : 300 u/ml
Elastase : 4 u/ml
Neutral Protease : 5 u/ml
Deoxyribonuclease I : 200 u/ml
Medium
HBSS
Lung
Mouse
Mouse, embryonic
Desai, T. , Brownfield, D. and Krasnow, M.
Alveolar Progenitor and Stem Cells in Lung Development, Renewal and Cancer. ,
Nature
 507 , 190-4 , 2014
Cells
Avelolar progenitor
Enzymes
Elastase : 3 u/ml
Deoxyribonuclease I : 0.33 u/ml
Medium
DMEM/F12
Lung
Mouse
Mouse
Rock, J, Barkauskas, C, Cronc, M, Xue, Y, Harris, J, Liang, J, Noble, P. and Hogan, B.
Multiple Stromal Populations Contribute to Pulmonary Fibrosis Without Evidence for Epithelial to Mesenchymal Transition. ,
Proc Natl Acad Sci U S A
 108 , E1475 , 2011
Cells
Lung
Enzymes
Collagenase Type 1 : 450 u/ml
Elastase : 4 u/ml
Neutral Protease : 5 u/ml
Deoxyribonuclease I : 0.33 u/ml
Medium
DMEM/F12
Lung
Rabbit
Rabbit, New Zealand, white, male, 1.7 Kg
Gould, M. , Clements, J. , Jones, A. and Felts, J.
Dispersal of Rabbit Lung into Individual Viable Cells: A New Model for the Study of Lung Metabolism ,
Science
 178 , 1209 , 1972
Cells
Lung
Enzymes
Pronase : 0.2%
Medium
Kreb's serum substitute solution, CMF
Lung
Rat
Rat, SD, 250-300g
Chen J. , Chen Z. , Narasaraju T. , Jin N. and Liu L.
Isolation of Highly Pure Alveolar Epithelial Type I and Type II Cells from Rat Lungs ,
Lab Invest
 84 , 727 , 2004
Cells
Alveolar epithelial
Enzymes
Elastase : 3-4.5 u/ml
Medium
RPMI 1640
Lung
Rat
Rat, SD, 125-150 g
Liebler, J. , Borok, Z. , Li, X. , Zhou, B. , Sandoval, A. and Kim, K.and Crandall, E.
Alveolar Epithelial Type I Cells Express Beta 2-Adrenergic Receptors and G-protein Receptor Kinase 2. ,
J Histochem Cytochem
 52 , 759 , 2004
Cells
Alveolar type I & II
Enzymes
Elastase : 2.5-8 u/ml
Medium
DMEM/F12
Lung
Rat
Rat, SD, 200-225 g
Sunil VR, Connor AJ, Guo Y, Laskin JD. and Laskin DL
Activation of type II alveolar epithelial cells during acute endotoxemia ,
Am J Physiol Lung Cell Mol Physiol
 282 , L872 , 2002
Cells
Type II alveolar epithelial cells
Enzymes
Elastase : 4.2 u/ml
Medium
DMEM
Lung
Rat
Rat, SD, male, adult
Kemp, P. , Kim, K. , Borok. and Crandall, E.
Re-evaluating the Na+ Conductance of Adult Rat Alveolar Type II Pneumocytes: Evidence for the Involvement of cGMP-Activated Cation Channels ,
J Physiol
 536 (3) , 693 , 2001
Cells
Alveolar
Enzymes
Elastase : 2.0 - 2.5 u/ml
Medium
DMEM/Ham's F-12
(see reference)
Lung
Rat
Rat, male
Planus, E. , Galiacy, S. , Matthay, M. , Laurent, V. , Gavrilovic, J. , Murphy, G. , Clerici, C. , Isabey, D. , Lafuma, C. and d'Ortho, M.
Role of Collagenase in Mediating in Vitro Alveolar Epithelial Wound Repair ,
J Cell Sci
 112 , 243 , 1999
Cells
Alveolar epithelial
Enzymes
Elastase : 40 u/ml
Medium
DMEM
Lung
Rat
Rat, male, 200-250g
Bakhramov, A, Evans, M. and Kozlowski, R
Differential effects of hypoxia on the intracellular Ca2+ concentration of myocytes isolated from different regions of the rat pulmonary arterial tree ,
Exp Physiol
 83 , 337-47 , 1998
Cells
Pulmonary arterial myocytes
Enzymes
Collagenase : 0.15%
Papain : 0.15%
Elastase : 0.05%
Medium
PBS
Lung
Rat
Rat, SD, male, 125-175 g
Kim, K. , Suh, D. , Lubman, R. , Danto, S. , Borok, Z. and Crandall, E.
Studies on the Mechanisms of Active Ion Fluxes Across Alveolar Epithelial Cell Monolayers ,
J Tiss Cul Meth
 14 , 187 , 1992
Cells
Alveolar epithelial
Enzymes
Elastase : 2 u/ml
Medium
EBSS
Lung
Rat
Rat, Fischer 344, male
Mangum, J. , Everitt, J. , Bonner, J. , Moore, L. and Brody, A.
Co-Culture of Primary Pulmonary Cells to Model Alveolar Injury and Translocation of Proteins ,
In Vitro Cell Dev Biol
 26 , 1135 , 1990
Cells
Alveolar type II pneumocytes
Enzymes
Elastase : 40 u/ml
Medium
HEPES buffer
(see reference)
Lung
Rat
Rat, SD, male, 250-400 g
Ma, J. , LaCagnin, L. , Bowman, L. and Miles, P.
Carbon Tetrachloride Inhibits Synthesis of Pulmonary Surfactant Disaturated Phosphatidylcholines and ATP Production in Alveolar Type II Cells ,
Biochim Biophys Acta
 1003 , 136 , 1989
Cells
Alveolar type II
Enzymes
Elastase : 40 u/ml
Medium
Phosphate-buffered medium
(see reference)
Lung
Rat
Rat, SD, male, 180-200 g
Dobbs, L. , Gonzalez R. and Williams, M.
An Improved Method for Isolating Type ll Cells in High Yield and Purity ,
Am Rev Respir Dis
 134 , 141 , 1986
Cells
Alveolar type II
Enzymes
Elastase : 4.3 u/ml
Medium
HEPES
(see reference)
Lung
Rat
Rat, SD, male, 150-400 g
Goodman, B. , Fleischer, R. and Crandall, E.
Evidence for Active Na+ Transport by Cultured Monolayers of Pulmonary Alveolar Epithelial Cells ,
Am J Physiol
 245 , C78 , 1983
Cells
Alveolar type II
Enzymes
Elastase : 4 u/ml
Medium
Auto-Pow Eagle's modified MEM
Mammary
Mouse
Mouse
Boulanger CA, Smith GH.
Reducing mammary cancer risk through premature stem cell senescence ,
Oncogene
 20 , 2264-72 , 2001
Cells
Mammary epithelial stem
Enzymes
Hyaluronidase : 0.1%
Pronase : 1.25%
Medium
DMEM
Mammary
Mouse
Mouse (BALB/cCrgl), mature
Bandyopadhyay, G. , Imagawa, W. , Wallace, D. and Nandi, S.
Proliferative effects of insulin and epidermal growth factor on mouse mammary epithelial cells in primary culture ,
J Biol Chem
 263 , 7567 , 1988
Cells
Epithelial
Enzymes
Pronase : 0.01%
Medium
Hepes buffered medium 199
Miscellaneous
Canine

Canine, Rat, Rabbit, Ovine, Bovine, Human

Basatvat, S. , Bach, F. , Barcellona, M. , Binch, A. , Buckley, C. , Bueno, B. , Chahine, N. , Chee, A. and Maitre, C.
Harmonization and Standardization of Nucleus Pulposus Cell Extraction and Culture Methods. ,
Spine
 6 , e1238 , 2023
Cells
nucleus pulposus
Enzymes
Pronase : 0.075%
Medium
DMEM
Miscellaneous
Human
Human
Sun, Y. , Lv, M. , Zhou, L. , Tam, V. , Lv, F. , Chan, D. , Wang, H. , Zheng, Z. , Cheung, K. and Leung, V.
Enrichment of Committed Human Nucleus Pulposus Cells Expressing Chondroitin Sulfate Proteoglycans under Alginate Encapsulation. ,
Osteoarthritis Cartilage
 23 , 1194-203 , 2015
Cells
Nucleus pulposus
Enzymes
Pronase : 0.25%
Collagenase Type 2 : 600 u/ml
Medium
DMEM
Miscellaneous
Human
Human
Ozdener, H. , Spielman, A. and Rawson, N.
Isolation and Culture of Human Fungiform Taste Papillae Cells ,
J Vis Exp
 63 , e3730 , 2012
Cells
Fungiform taste papillae
Enzymes
Medium
see reference
Miscellaneous
Mouse
Mouse, embryonic
He, M. , Wu, B. , Ye, W. , Le, D. , Sinclair, A. , Padovano, V. , Chen, Y. , Li, K. , Sit, R. , Tan, M. , Caplan, M. , Neff, N. , Jan, Y. , Darmanis, S. and Jan, L.
Chloride Channels Regulate Differentiation and Barrier Functions of the Mammalian Airway. ,
Elife
 9 , 53085 , 2020
Cells
Trachea
Enzymes
Elastase : 0.1%
Trypsin : 0.125%
Medium
DMEM/F12
Miscellaneous
Porcine
Porcine, <10 week
Potier, E. and Ito. K.
Can Notochordal Cells Promote Bone Marrow Stromal Cell Potential for Nucleus Pulposus Enrichment? A Simplified in Vitro System. ,
Tissue Eng Part A
 20 , 3241-51 , 2014
Cells
Nucleus pulposus
Enzymes
Collagenase : 0.05%
Pronase : 0.3%
Medium
DMEM
Miscellaneous
Porcine
Porcine, various ages
Cho, H. , Park, S. , Lee, S. , Kang, M. , Hasty, K. and Kim, S.
Snapshot of Degenerative Aging of Porcine Intervertebral Disc: A Model to Unravel the Molecular Mechanisms. ,
Exp Mol Med
 43 , 334-40 , 2011
Cells
Nucleous pulposus and AF
Enzymes
Pronase : 0.05%
Collagenase : 0.2%
Medium
Modified F-12K
Muscle
Canine
Dog
Subramanian, M. , Madden, J. and Harder, D.
A Method for the Isolation of Cells from Arteries of Various Sizes ,
J Tiss Cul Meth
 13 , 13 , 1991
Cells
Smooth muscle
Enzymes
Elastase : 50 u/ml
Medium
PSS
Muscle
Canine
Dog, beagle, adult
Wilde, D. and Lee, K.
Outward Potassium Currents in Freshly Isolated Smooth Muscle Cell of Dog Coronary Arteries ,
Circ Res
 65 , 1718 , 1989
Cells
Smooth muscle
Vascular
Enzymes
Elastase : 34 u/ml
Medium
Tyrode's solution w/ calcium
Muscle
Canine
Dog
Dobrin, P. and Canfield, T.
Elastase, Collagenase, and the Biaxial Elastic Properties of Dog Carotid Artery ,
Am J Physiol Heart Circ Physiol
 247 (16) , H124 , 1984
Cells
Artery
Carotid
Enzymes
Elastase : 80 u/ml
Medium
PSS
Muscle
Feline
Cat, adult mongrel, either sex, 2.5-4.0 kg
Madden, J. , Vadula, M. and Kurup, V.
Effects of Hypoxia and Other Vasoactive Agents on Pulmonary and Cerebral Artery Smooth Muscle Cells ,
Am J Physiol
 263 , L384 , 1992
Cells
Cerebral arteries
Enzymes
Elastase : 50 u/ml
Medium
Puck's solution
Muscle
Monkey
Rhesus monkey, 1 year
(also human, rabbits)
Chamley, J. , Campbell, G. , McConnell, J. and Groschel-Stewart, U.
Comparison of Vascular Smooth Muscle Cells from Adult Human, Monkey and Rabbit in Primary Culture and in Subculture ,
Cell Tissue Res
 177 , 503 , 1977
Cells
Smooth muscle, saphenous vein
Enzymes
Elastase : 0.05%
Medium
BSS
Muscle
Mouse
Mouse
Eckhardt, J. , Bachmann, C. , Sekulic-Jablanovic, M. , Enzmann, V. , Park, K. , Ma, J. , Takeshima, H. , Zorzato, F. and Treves, S.
Extraocular Muscle Function is Impaired in ryr3−/− Mice ,
J Gen Physiol
 151 , 929-943 , 2019
Cells
Extraocular muscles
Enzymes
Elastase : 0.0125-0.1%
Collagenase Type 2 : 0.03-0.2%
Medium
Ringer

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.