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Tissue References Search Tool

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Heart
Bovine
Bovine
(also rat)
Trifaro, J. , Tang, R. and Novas, M.
Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons ,
In Vitro Cell Dev Biol
 26 , 335 , 1990
Cells
Heart
Adrenal chromaffin
Paraneurons
Enzymes
Trypsin : 0.06%
Medium
25mM HEPES buffered Locke's solution, CMF
Heart
Chicken
Chicken, embryonic
Blech-Hermoni, Y. and Ladd, A.
Identification of Transcripts Regulated by CUG-BP, Elav-like Family Member 1 (CELF1) in Primary Embryonic Cardiomyocytes by RNA-seq. ,
Genom Data
 6 , 74-76 , 2015
Cells
Cardiomyocytes
Enzymes
Medium
HBSS
Heart
Chicken
Chicken embryos, 9-11 day incubated
Eschenhagen, T. , Fink, C. , Remmers, U. , Scholz, H. , Wattchow, J. , Weil, J. and et al.
Three-Dimensional Reconstitution of Embryonic Cardiomyocytes in a Collagen Matrix: A New Heart Muscle Model System ,
FASEB J
 11 , 683 , 1997
Cells
Cardiomyocytes
Enzymes
Trypsin : 0.25%
Medium
PBS
Heart
Chicken
Chick, embryos, 10-11 day
Wang, S. , Greaser, M.L. , Schultz, E. , Bulinski, J.C. , Lin, J.J. and Lessard, J.L.
Studies on Cardiac Myofibrillogenesis With Antibodies to Titin, Actin, Tropomyosin, and Myosin ,
J Cell Biol
 107 , 1075 , 1988
Cells
Cardiomyocytes
Enzymes
Trypsin : 0.17%
Medium
HBSS, CMF
Heart
Chicken
Chick, 10 day
Kim, D. , Okada, A. and Smith, T.W.
Control of Cytosolic Calcium Activity During Low Sodium Exposure in Cultured Chick Heart Cells ,
Circ Res
 61 , 29 , 1987
Cells
Ventricular
Enzymes
Trypsin : 0.025%
Medium
HBSS, CMF
Heart
Chicken
Chick, embryo, 11 day
Jacob, R. , Lieberman, M. , Murphy, E. and Piwnica-Worms, D.
Effect of Sodium-Potassium Pump Inhibition and Low Sodium on Membrane Potential in Cultured Embryonic Chick Heart Cells ,
J Physiol
 387 , 549 , 1987
Cells
Cardiomyocytes
Enzymes
Trypsin : 0.05%
Medium
HBSS, CMF
Heart
Chicken
Chick, embryonic, 11-12-day-old
Murphy, E. , Aiton, J. , Russell, C. and Lieberman, M.
Calcium Elevation in Cultured Heart Cells: Its Role in Cell Injury ,
Am J Physiol Cell Physiol
 245 (14) , C316 , 1983
Cells
Myocytes
Enzymes
Trypsin : 0.025%
Medium
CMF solution
Heart
Chicken
Hen, White Leghorn
Dehann, R.
Regulation of Spontaneous Activity and Growth of Embryonic Chick Heart Cells in Tissue Culture ,
Dev Biol
 16 , 216 , 1967
Cells
Heart
Enzymes
Trypsin : 0.025%
Medium
Medium 199
Heart
Chicken
Chick embryos, 5 day
Steinberg, M.
"ECM": Its Nature, Origin, And Function in Cell Aggregation ,
Exp Cell Res
 30 , 257 , 1963
Cells
Heart
Liver
Enzymes
Trypsin : 3.0%
Medium
Tyrode's solution, CMF
Heart
Fish
Zebrafish
Fowler, E. , Wang, N. , Hezzell, M. , Chanoit, G. , Hancox, J. and Cannell, M.
Arrhythmogenic Late Ca2+ Sparks in Failing Heart Cells and Their Control by Action Potential Configuration ,
Proc Natl Acad Sci U S A
 117 , 2687-2692 , 2020
Cells
Cardiac Myocytes
Enzymes
Trypsin : 0.08%
Protease XIV : 0.005%
Collagenase Type 1 : 0.1-0.2%
Medium
HEPES
Heart
Frog
Frog (Rana esculenta)
Arrio-Dupont, M. and de Nay, D.
High Yield Preparation of Calcium-Tolerant Myocytes From Frog Ventricles ,
Biol Cell
 54 , 164 , 1985
Cells
Myocytes
Enzymes
Trypsin : 0.04%
Medium
CF Ringer
Heart
Human
Human
Sharma, S. , Mishra, R. , Simpson, D. , Wehman, B. , Colletti, E. , Deshmukh, S. , Datla, S. , Balachandran, K. , Guo, Y. , Chen, L. , Siddiqui, O. , Kaushal, S. and Kaushal, S.
Cardiosphere-Derived Cells From Pediatric End-Stage Heart Failure Patients Have Enhanced Functional Activity Due to the Heat Shock Response Regulating the Secretome. ,
Stem Cells
 33 , 1213-29 , 2015
Cells
Cardiospheres
Enzymes
Medium
Iscove modified DMEM
Heart
Human
Human
Jensen, B. , Swigart, P. , Laden, M. , DeMarco, T. , Hoopes, C. and Simpson, P.
The Alpha-1D Is the Predominant Alpha-1-Adrenergic Receptor Subtype in Human Epicardial Coronary Arteries. ,
J Am Coll Cardiol
 54 , 1137 , 2009
Cells
Coronary artery smooth muscle
Enzymes
Elastase : 0.05%
Medium
DMEM
Heart
Human
Human
Mathew, S. , Tustison, K. , Sugatani, T. , Chaudhary, L. , Rifas, L. and Hruska, K.
The Mechanism of Phosphorus as a Cardiovascular Risk Factor in CKD. ,
J Am Soc Nephrol
 19 , 1092 , 2008
Cells
Smooth muscle aortic
Enzymes
Medium
DMEM
Heart
Human
Human
Shirai, T. , Rao, V. , Weisel, R. , Ikonomidis, J. , Li, R. , Tumiati, L. , Merante, F. and Mickle, D.
Preconditioning Human Cardiomyocytes and Endothelial Cells. ,
J Thorac Cardiovasc Surg
 115 , 210-9 , 1998
Cells
Cardiomyocytes and endothelial
Enzymes
Trypsin : 0.2%
Collagenase : 0.1%
Medium
PBS
Heart
Human
Human, adult
Bugaisky, L.B.
Biology of Isolated Adult Cardiac Myocytes , Isolation and Culture of Human Adult Cardiac Myocytes , (Clark,Decker,Borg,eds) , Elsevier Science Publishing Co., Inc. , 1988
Cells
Myocytes
Enzymes
Trypsin : 0.25%
Medium
Joklik's MEM
Heart
Invertebrate
Octopus, E. cirhosa, 260-352 g
Altimiras J. , Hove-Madsen L. and Gesser H.
Ca(2+) Uptake in the Sarcoplasmic Reticulum from the Systemic Heart of Octopod Cephalopods ,
J Exp Biol
 202 , 2531 , 1999
Cells
Systemic heart cardiomyocytes
Enzymes
Collagenase : 0.025%
Trypsin : 0.02%
Medium
see reference
Heart
Mouse

Mouse, neonatal

Angelini, A. , Pi, X. and Xie, L.
Evaluation of Long-Chain Fatty Acid Respiration in Neonatal Mouse Cardiomyocytes using SeaHorse Instrument. ,
Star Protocols
 3 , 101392 , 2022
Cells
Cardiomyocytes
Enzymes
Trypsin : 0.05%
CLSPA : 150 u/ml
Medium
L-15
Heart
Mouse
Mouse, 2-6 month
Christel, C. , Cardona, N. , Mesirca, P. , Herrmann, S. , Hofmann, F. , Striessnig, J. , Ludwig, A. , Mangoni, M. and Lee, A.
Distinct Localization and Modulation of Cav1.2 and Cav1.3 L-type Ca2+ Channels in Mouse Sinoatrial Node. ,
J Physiol
 590 , 6327 , 2012
Cells
Sinoatrial node
Enzymes
Collagenase Type 2 : 229 u/ml
Elastase : 1.9 u/ml
Protease Type XIV : 0.9 u/ml
Medium
Tyrode's solution
Heart
Mouse
Mouse
Butcher, M. , Herre, M. , Ley, K. and Galkina, E.
Flow Cytometry Analysis of Immune Cells Within Murine Aortas. ,
J Vis Exp
 53 , e2828 , 2011
Cells
Aortic adventitial leukocytes
Enzymes
Collagenase Type 2 : 300 u/ml
Elastase : 5.6 u/ml
Medium
PBS
Heart
Mouse
Mouse
Verheye, S. , Martinet, W. , Kockx, M. , Knaapen, M. , Salu, K. , Timmermans, J. , Ellis, J. , Kilpatrick, D. and DeMayer, G.
Selective Clearance of Macrophages in Atherosclerotic Plaques by Autophagy ,
J Am Coll Cardiol
 49 , 706 , 2007
Cells
Smooth muscle cells
Enzymes
Collagenase Type 2 : 300 u/ml
Elastase : 5 u/ml
Medium
F10 Ham
Heart
Mouse
Mouse, adult, 6-12 week
Shioya, T.
A Simple Technique for Isolating Healthy Heart Cells from Mouse Models. ,
J Physiol Sci
 57 , 327-35 , 2007
Cells
Cardiomyocytes
Enzymes
Trypsin : 0.006%
Protease XIV : 0.006%
Medium
see reference
Heart
Mouse
Mouse, neonatal
Takahashi N, Wang X, Tanabe S, Uramoto H, Jishage K, Uchida S, Sasaki S, Okada Y.
ClC-3-independent sensitivity of apoptosis to Cl- channel blockers in mouse cardiomyocytes ,
Cell Physiol Biochem
 15 , 263 , 2005
Cells
Cardiomyoctes
Enzymes
Collagenase Type 1 : 150 u/ml
Trypsin : 0.01%
Medium
M199
Heart
Mouse
Mouse
Fukumoto Yoshihiro, Deguchi Jun-o, Libby Peter, Rabkin-Aikawa Elena, Sakata Yasuhiko, Chin Michael T, Hill Christopher C, Lawler Patrick R, Varo Nerea, Schoen Frederick J, Krane Stephen M, Aikawa Masanori.
Genetically determined resistance to collagenase action augments interstitial collagen accumulation in atherosclerotic plaques ,
Circulation
 110 , 1953-9 , 2004
Cells
Smooth muscle cells
Enzymes
Elastase : 0.0125%
Medium
DMEM
Heart
Mouse
Mouse, female, 14-18 g
Zhang Sui, Wang Dachun, Estrov Zeev, Raj Sean, Willerson James T, Yeh Edward T H.
Both cell fusion and transdifferentiation account for the transformation of human peripheral blood CD34-positive cells into cardiomyocytes in vivo ,
Circulation
 110 , 3803-7 , 2004
Cells
Cardiomyocytes
Enzymes
Elastase : 0.03%
Pancreatin : 0.06%
Medium
see reference
Heart
Mouse
Mouse, 6-8 week
Qian, Q. , Hunter, L. , Li, M. , Marin-Padilla, M. , Prakash, Y. , Somlo, S. , Harris, P. , Torres, V. and Sieck, G.
Pkd2 Haploinsufficiency Alters Intracellular Calcium Regulation in Vascular Smooth Muscle Cells ,
Hum Mol Genet
 12(15) , 1875 , 2003
Cells
Vascular smooth muscle
Enzymes
Papain : 10 u/ml
Elastase : .005%
Collagenase : 0.05%
Deoxyribonuclease I : 1000 u/ml
PDS kit : with modifications
Medium
EBSS
Heart
Mouse
Mouse, 3-7 month
Bradshaw AD, Francki A, Motamed K, Howe C, Sage EH.
Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation ,
Mol Biol Cell
 10 , 1569-79 , 1999
Cells
Fibroblasts, mesangial, smooth muscle
Enzymes
Trypsin : 0.25%
Collagenase : see reference
Medium
DMEM
Heart
Mouse
Mouse, 1-3 day
Wang, G. and Kang, Y.
Inhibition of Doxorubicin Toxicity in Cultured Neonatal Mouse Cardiomyocytes with Elevated Metallothionein Levels ,
J Pharmacol Exp Ther
 288(3) , 938 , 1999
Cells
Cardiomyocytes
Enzymes
Trypsin : 0.25%
Medium
FBS-MEM
Heart
Mouse
Mouse, 6-9 or 21-41 day
Valenzuela, D. , Han, X. , Mende, U. , Fankhauser, C. , Mashimo, H. , Huang, P. , Pfeffer, J. , Neer, E. and Fishman, M.
G alpha(o) is Necessary for Muscarinic Regulation of Ca2+ Channels in Mouse Heart ,
Proc Natl Acad Sci U S A
 94(5) , 1727 , 1997
Cells
Ventricular and atrial myocytes
Enzymes
Collagenase Type 2 : 95 u/ml
Hyaluronidase : 172.5 u/ml
Trypsin : 0.002%
Medium
DMEM/Tyrodes
Heart
Porcine
Porcine
Christ, M. , Gunther, A. , Heck, M. , Schmidt, B. , Falkenstein, E. and Wehling, M.
Aldosterone, Not Estradiol, Is the Physiological Agonist for Rapid Increases in cAMP in Vascular Smooth Muscle Cells ,
Circulation
 99 , 1485 , 1999
Cells
Coronary vascular smooth muscle (PCVSMCs)
Enzymes
Trypsin : 0.037%
Medium
HEPES
Heart
Porcine
Miniature swine, adult, female, 25-40 kg, familiarized with treadmill exercise
Bowles, D. , Hu, Q. , Laughlin, M. and Sturek, M.
Exercise Training Increases L-type Calcium Current Density in Coronary Smooth Muscle ,
Am J Physiol
 275 (44) , H2159 , 1998
Cells
Coronary smooth muscle
Enzymes
Trypsin : 0.1%
Medium
Krebs bicarbonate solution
Heart
Rabbit
Rabbit, New Zealand, 3-3.5 kg
Fowler, E. , Wang, N. , Hezzell, M. , Chanoit, G. , Hancox, J. and Cannell, M.
Arrhythmogenic Late Ca2+ Sparks in Failing Heart Cells and Their Control by Action Potential Configuration ,
Proc Natl Acad Sci U S A
 117 , 2687-2692 , 2020
Cells
Cardiac Myocytes
Enzymes
Trypsin : 0.08%
Protease XIV : 0.005%
Collagenase Type 1 : 0.1-0.2%
Medium
HEPES
Heart
Rabbit
Rabbit
Verheye, S. , Martinet, W. , Kockx, M. , Knaapen, M. , Salu, K. , Timmermans, J. , Ellis, J. , Kilpatrick, D. and DeMayer, G.
Selective Clearance of Macrophages in Atherosclerotic Plaques by Autophagy ,
J Am Coll Cardiol
 49 , 706 , 2007
Cells
Smooth muscle cells
Enzymes
Collagenase Type 2 : 300 u/ml
Elastase : 5 u/ml
Medium
F10 Ham
Heart
Rat
Rat, adult
Zhang, Y. , Wang, Y. , Yanni, J. , Qureshi, M. , Logantha, S. , Kassab, S. , Boyett, M. , Gardiner, N. , Sun, H. , Howarth, F. and Dobrzynski, H.
Electrical Conduction System Remodeling in Streptozotocin-Induced Diabetes Mellitus Rat Heart. ,
Front Physiol
 10 , 826 , 2019
Cells
Sinoatrial nodal
Enzymes
Collagenase Type 4 : 220 u/ml
Elastase : 1.4 u/ml
Protease : 0.45 u/ml
Medium
Tyrode solution
Heart
Rat
Rat, SD, 1 day
Clark, A. and Naya, F.
MicroRNAs in the Myocyte Enhancer Factor 2 (MEF2)-regulated Gtl2-Dio3 Noncoding RNA Locus Promote Cardiomyocyte Proliferation by Targeting the Transcriptional Coactivator Cited2. ,
J Biol Chem
 290 , 23162-72 , 2015
Cells
Cardiomyocytes
Enzymes
Trypsin : 0.025%
Medium
HBSS
Heart
Rat
Rat, neonatal
Zhang, B. , Montgomery, M. , Davenport-Huyer, L. , Korolj, A. and Radisic, M.
Platform Technology for Scalable Assembly of Instantaneously Functional Mosaic Tissues. ,
Sci. Adv.
 1 , e1500423 , 2015
Cells
Cardiomyocytes
Enzymes
Trypsin : 0.06%
Collagenase Type 2 : 220 u/ml
Medium
HBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.