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Tissue References Search Tool

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Stem
Human
Human
de Witte, S. , Lambert, E. , Merino, A. , Strini, T. , Douben, H. , O'Flynn, L. , Elliman, S. , de Klein, A. , Newsome, P. , Baan, C. and Hoogduijn, M.
Aging of Bone Marrow- and Umbilical Cord-Derived Mesenchymal Stromal Cells During Expansion. ,
Cytotherapy
 19 , 798-807 , 2017
Cells
Umbilical cord mesenchymal stromal
Enzymes
Medium
MEM
Stem
Human
Human
Rigamonti, A. , Repetti, G. , Sun, C. , Price, F. , Reny, D. , Rapino, F. , Weisinger, K. , Benkler, C. , Peterson, Q. , Davidow, L. , Hansson, E. and Rubin, L.
Large-Scale Production of Mature Neurons from Human Pluripotent Stem Cells in a Three-Dimensional Suspension Culture System. ,
Stem Cell Reports
 6 , 993-1008 , 2016
Cells
Motor neurons
Enzymes
Papain : 20 u/ml
Medium
EBSS
Stem
Human
Human
Dobbin, Z. and Landen, C.
Isolation and Characterization of Potential Cancer Stem Cells from Solid Human Tumors--Potential Applications. ,
Curr Protoc Pharmacol
 63 , Unit 14.28. , 2013
Cells
Cancer stem
Enzymes
Trypsin : 0.25%
Collagenase : 0.05%
Hyaluronidase : 0.005%
Medium
RPMI
Stem
Human
Human, fetal
Vatakis, D. , Bristol, G. , Kim, S. , Levin, B. , Liu, W. , Radu, C. , Kitchen, S. and Zack, J.
Using the BLT Humanized Mouse as a Stem Cell Based Gene Therapy Tumor Model. ,
J Vis Exp
 70 , e4181 , 2012
Cells
Liver hematopoietic
Enzymes
Medium
RPMI
Stem
Human
Hussein, D. , Punjaruk, W. , Storer, L. , Shaw, L. , Othman, R. , Ottoman, R. , Peet, A. , Miller, S. , Bandopadhyay, G. , Heath, R. , Kumari, R. , Bowman, K. , Braker, P. , Rahman, R. , Jones, G. , Watson, S. and Lowe, J.
Pediatric Brain Tumor Cancer Stem Cells: Cell Cycle Dynamics, DNA Repair, and Etoposide Extrusion. ,
Neuro Oncol
 13 , 70-83 , 2011
Cells
Brain tumor
Enzymes
Medium
DMEM/F12
Stem
Human
Human
Farias, V. , Linares-Fernandez, J. , Penalver, J. , Paya Colmenero, J. , Ferron, G. , Duran, E. , Fernandez, R. , Olivares, E. , O'Valle, F. , Puertas, A. , Oliver, F. and Ruiz de Almodovar, J.
Human Umbilical Cord Stromal Stem Cell Express CD10 and Exert Contractile Properties. ,
Placenta
 32 , 86 , 2011
Cells
Umbilical cord stromal stem
Enzymes
Medium
DMEM
Stem
Human
Human
Zeddou, M. , Briquet, A. , Relic, B. , Josse, C. , Malaise, M. , Gothot, A. , Lechanteur, C. and Beguin, Y.
The Umbilical Cord Matrix is a Better Source of Mesenchymal Stem Cells (MSC) Than the Umbilical Cord Blood. ,
Cell Biol Int
 34 , 693-701 , 2010
Cells
Umbilical cord mesenchymal
Enzymes
Hyaluronidase : 0.05%
Collagenase : 0.08%
Medium
DMEM
Stem
Human
Human
Sauvageot, C. , Weatherbee, J. , Kesari, S. , Winters, S. , Barnes, J. , Dellagatta, J. , Ramakrishna, N. , Stiles, C. , Kung, A. , Kieran, M. and Wen, P.
Efficacy of the HSP90 Inhibitor 17-AAG in Human Glioma Cell Lines and Tumorigenic Glioma Stem Cells. , Neuro Oncol Vol. 11 , 109 , 2009
Cells
Tumor
Enzymes
Medium
see reference
Stem
Human
Human
Varnat, F. , Duquet, A. , Malerba, M. , Zbinden, M. , Mas, C. , Gervaz, P. and Ruiz i Altaba, A.
Human Colon Cancer Epithelial Cells Harbour Active HEDGEHOG-GLI Signalling that is Essential for Tumour Growth, Recurrence, Metastasis and Stem Cell Survival and Expansion. ,
EMBO Mol Med
 1 , 338-51 , 2009
Cells
Colon cancer
Enzymes
Collagenase Type 1 : 300 u/ml
Hyaluronidase : 100 u/ml
Medium
DMEM/F12
Stem
Human
Human
Welter, J. and Solchaga, L.and Penick, K.
Simplification of Aggregate Culture of Human Mesenchymal Stem Cells as a Chondrogenic Screening Assay ,
Biotechniques
 42 , 732 , 2007
Cells
Bone marrow derived MSC
Enzymes
Trypsin : 0.05%
Papain : 0.0025%
Medium
DMEM
Stem
Human
Human, adult
Kambe, N, Kambe, M, Kochan, J. and Schwartz ,L.
Human Skin-derived Mast Cells Can Proliferate While Retaining Their Characteristic Functional and Protease Phenotypes ,
Blood
 97 , 2045 , 2001
Cells
Human skin mast cells
Enzymes
Medium
HBSS
Stem
Human
Human
Uchida, N. , Buck, D. , He, D. , Reitsma, M. , Masek, M. , Phan, T. , Tsukamoto, A. , Gage, F. and Weissman, I.
Direct Isolation of Human Central Nervous System Stem Cells. ,
Proc Natl Acad Sci U S A
 97 , 14720 , 2000
Cells
Central nervous system stem
Enzymes
Collagenase : 0.1%
Hyaluronidase : 0.1%
Medium
HBSS
Stem
Insect
Drosophila, larval
Berger, C. , Harzer, H. , Burkard, T. , Steinmann, J. , van der Horst, S. , Laurenson, A. , Novatchkova, M. , Reichert, H. and Knoblich, J.
FACS Purification and Transcriptome Analysis of Drosophila Neural Stem Cells Reveals a Role for Klumpfuss in Self-Renewal. ,
Cell Rep
 2 , 407 , 2012
Cells
Neuroblasts
Enzymes
Papain : 0.1%
Medium
Rinaldini solution
Stem
Mouse
Mouse
Liu, X. , Johnson, S. , Liu, S. , Kanojia, D. , Yue, W. , Singh, U. , Wang, Q, Wang Qi, Nie, Q. and Chen H.
Nonlinear Growth Kinetics of Breast Cancer Stem Cells: Implications for Cancer Stem Cell Targeted Therapy. ,
Sci Rep
 3 , 2473 , 2013
Cells
Mammary tumor
Enzymes
Collagenase : 0.15%
Hyaluronidase : 0.020%
Medium
DMEM/F12
Stem
Mouse
Mouse, 6-8 week
Bracko, O. , Singer, T. , Aigner, S. , Knobloch, M. , Winner, B. , Ray, J. , Clemenson, G. , Suh, H. , Couillard-Despres, S. , Aigner, L. , Gage, F. and Jessberger, S.
Gene Expression Profiling of Neural Stem Cells and their Neuronal Progeny Reveals IGF2 as a Regulator of Adult Hippocampal Neurogenesis. ,
J Neurosci
 32 , 3376-87 , 2012
Cells
Neural stem cells
Enzymes
Medium
DMEM/F12
Stem
Mouse
Mouse, P4-5
Ziegler, A. , Schneider, J. , Qin, M. , Tyler, W. , Pintar, J. , Fraidenraich, D. , Wood, T. and Levison, S.
IGF-II Promotes Stemness of Neural Restricted Precursors. ,
Stem Cells
 30 , 1265 , 2012
Cells
Neuroshere
Enzymes
Trypsin : 0.25%
Papain : 100 u
Medium
Pro-N
Stem
Mouse
Mouse, 12 week
Taddei, I. , Deugnier, M. , Faraldo, M. , Petit, V. , Bouvard, D. , Medina, D. , Fassler, R. , Thiery, J. and Glukhova, M.
Beta1 Integrin Deletion from the Basal Compartment of the Mammary Epithelium Affects Stem Cells ,
Nat Cell Biol
 10 , 716-22 , 2008
Cells
Mammary epithelial
Enzymes
Collagenase : 0.3%
Hyaluronidase : 100 u/ml
Trypsin : 0.25%
Medium
see reference
Stem
Mouse
Mouse
Hutton, S. and Pevny, L.
Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous System. ,
Cold Spring Harb. Protoc.
 11 , 5077 , 2008
Cells
Neural progenitor cell
Enzymes
Papain : 10 ul/ml
Medium
PBS
Stem
Mouse
Mouse
Meletis, K. , Wirta, V. , Hede, S. , Nister, M. and Lundeberg, J. abd Frisen, J.
p53 Suppresses the Self-Renewal of Adult Neural Stem Cells. ,
Development
 133 , 363 , 2006
Cells
Neural stem cells
Enzymes
Papain : see reference
Medium
DMEM/F-12
Stem
Mouse
Mouse, day 7
Lee, A, Kessler, J, Read, T, Kaiser, C, Corbeil, D, Huttner ,W, Johnson, J, Wechsler-Reya, R.
Isolation of Neural Stem Cells from the Postnatal Cerebellum ,
Nat Neurosci
 8 , 723 , 2005
Cells
Cerebellar stem cells
Enzymes
Papain : 10u/ml
Deoxyribonuclease I : 250 u/ml
Medium
Dulbecco's PBS
Stem
Mouse
Mouse, 15 day or 3 month
Gritti, A, Bonfanti, L, Doetsch, F, Caille, I, Alvarez-Buylla, A, Lim, D, Galli, R, Verdugo J, Herrera, D. and Vescovi A.
Multipotent Neural Stem Cells Reside into the Rostral Extension and Olfactory Bulb of Adult Rodents ,
J Neurosci
 22(2) , 437 , 2002
Cells
Neural stem cells
Enzymes
Papain : 0.1%
Medium
DMEM/F-12
Stem
Mouse
Mouse
Boulanger CA, Smith GH.
Reducing mammary cancer risk through premature stem cell senescence ,
Oncogene
 20 , 2264-72 , 2001
Cells
Mammary epithelial stem
Enzymes
Hyaluronidase : 0.1%
Pronase : 1.25%
Medium
DMEM
Stem
Mouse
Mouse, 4-8 month
Gritti A, Frolichsthal-Schoeller P, Galli R, Parati E, Cova L, Pagano S, Bjornson C. and Vescovi A.
Epidermal and Fibroblast Growth Factors Behave as Mitogenic Regulators for a Single Multipotent Stem Cell-like Population from the Subventricular Region of the Adult Mouse forebrain ,
J Neurosci
 19(9) , 3287 , 1999
Cells
Neural subventricular zone
Enzymes
Trypsin : 0.13%
Hyaluronidase : 0.067%
Medium
DMEM/F12
Stem
Porcine
Porcine, 1-4 day
Park, M. , Park, J. , Kim, M. , Lee, K. , Park, H. , Yun, J. , Choi, J. , Lee, E. and Lee, S.
Development of a High-Yield Technique to Isolate Spermatogonial Stem Cells From Porcine Testes. ,
J Assist Reprod Genet
 31 , 983-91 , 2014
Cells
Testicular
Enzymes
Hyaluronidase : 0.1%
Trypsin : 0.25%
Medium
DMEM
Stem
Porcine
Porcine, male, 8 day
Dirami G, Ravindranath N, Pursel V, Dym M.
Effects of stem cell factor and granulocyte macrophage-colony stimulating factor on survival of porcine type A spermatogonia cultured in KSOM ,
Biol Reprod
 61 , 225-30 , 1999
Cells
Seminiferous epithelial cells
Enzymes
Collagenase : 0.15%
Hyaluronidase : 0.15%
Trypsin : 0.05%
Medium
DMEM/F12
Stem
Rat
Rat, adult
Duarte, S. , Momier, D. , Baque, P. , Casanova, V. , Loubat, A. , Samson, M. , Guigonis, J. , Staccini, P. , Saint-Paul, M. , De Lima, M. , Carle, G. and Pierrefite-Carle, V.
Preventive Cancer Stem Cell-Based Vaccination Reduces Liver Metastasis Development in a Rat Colon Carcinoma Syngeneic Model. ,
Stem Cells
 31 , 423-32 , 2013
Cells
Tumor
Enzymes
Medium
DMEM/F12
Stem
Rat
Rat, male, 200-250 g
Gobbel GT, Choi SJ, Beier S, Niranjan A.
Long-term cultivation of multipotential neural stem cells from adult rat subependyma ,
Brain Res
 980 , 221 , 2003
Cells
Neural stem cells
Enzymes
Papain : 0.09%
Medium
EBSS
Stem
Rat
Rat, adult
Palmer, T. , Markakis, E. , Willhoite, A. , Safar, F. and Gage, F.
Fibroblast Growth Factor-2 Activates a Latent Neurogenic Program in Neural Stem Cells from Diverse Regions of the Adult CNS ,
J Neurosci
 19 , 8487 , 1999
Cells
Neural stem cells
Enzymes
Papain : 2.5 u/ml
Deoxyribonuclease I : 250 u/ml
Neutral Protease : 1 u/ml
Medium
DMEM/F-12
Stem
Rat
Rat
Ray, J. and Gage, F.
Neural Stem Cell Isolation, Characterization and Transplantation , Modern Techniques in Neuroscience Research , Johansson, H. and Windhorst, U. , Springer-Verlag New York , 339 , 1999
Cells
Central nervous system stem cells
Enzymes
Papain : 0.01%
Medium
DMEM/F-12
Stem
Rat
Rat, embryonic
Maric, D. , Maric, I. and Barker, J.
Buoyant Density Gradient Fractionation and Flow Cytometric Analysis of Embryonic Rat Cortical Neurons and Progenitor Cells. ,
Methods
 16 , 247 , 1998
Cells
Neurons and progenitor
Enzymes
Papain : 20 u/ml
Medium
EBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.