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Tissue References Search Tool

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Neural
Mouse
Mouse, 4 month
Tongtako, W. , Lehmbecker, A. , Wang, Y. , Hahn, K. , Baumgartner, W. and Gerhauser, I.
Canine Dorsal Root Ganglia Satellite Glial Cells Represent an Exceptional Cell Population with Astrocytic and Oligodendrocytic Properties. ,
Sci Rep
 7 , 13915 , 2017
Cells
Satellite glial
Enzymes
Medium
HBSS
Neural
Mouse
Mouse, neonatal
Stettner, M. , Lohmann, B. , Wolffram, K. , Weinberger, J. , Dehmel, T. , Hartung, H. , Mausberg, A. and Kieseier, B.
Interleukin-17 Impedes Schwann Cell-Mediated Myelination. ,
J Neuroinflammation
 11 , 63 , 2014
Cells
Schwann cells
Enzymes
Collagenase : 0.05-0.1%
Trypsin : 0.125-0.25%
Medium
DMEM
Neural
Mouse
Mouse
Lee, B. , Cho, H. , Jung, J. , Yang, Y. , Yang, D. and Oh, U.
Anoctamin 1 Contributes to Inflammatory and Nerve-Injury Induced Hypersensitivity. ,
Mol Pain
 10 , 5 , 2014
Cells
DRG neurons
Enzymes
Trypsin : 0.25%
Medium
DMEM/F-12
Neural
Mouse
Mouse
Zhang, Y. and Hu, W.
Mouse Enteric Neuronal Cell Culture. ,
Methods Mol Biol
 1078 , 55-63 , 2013
Cells
Enteric neurons
Enzymes
Trypsin : 0.05%
Deoxyribonuclease I : 400 u/ml
Medium
DMEM/F12
Neural
Mouse
Mouse, 4-6 week
Masaki, T. , Qu, J. , Cholewa-Waclaw, J. , Burr, K. , Raaum, R. and Rambukkana, A.
Reprogramming Adult Schwann Cells to Stem Cell-Like Cells by Leprosy Bacilli Promotes Dissemination of Infection. ,
Cell
 152 , 51 , 2013
Cells
Schwann
Enzymes
Trypsin : 0.125%
Medium
DMEM
Neural
Mouse
Mouse, 6-8 week
Babona-Pilipos, R. , Popovic, M. and Morshead, C.
A Galvanotaxis Assay for Analysis of Neural Precursor Cell Migration Kinetics in an Externally Applied Direct Current Electric Field. ,
J Vis Exp
 68 , e4193 , 2012
Cells
Neural precursors
Enzymes
Trypsin : 0.13%
Hyaluronidase : 0.08%
Medium
see reference
Neural
Mouse
Mouse, P4-5
Ziegler, A. , Schneider, J. , Qin, M. , Tyler, W. , Pintar, J. , Fraidenraich, D. , Wood, T. and Levison, S.
IGF-II Promotes Stemness of Neural Restricted Precursors. ,
Stem Cells
 30 , 1265 , 2012
Cells
Neuroshere
Enzymes
Trypsin : 0.25%
Papain : 100 u
Medium
Pro-N
Neural
Mouse
Mouse, embryonic
Conrad, R. , Jablonka, S. , Sczepan, T. , Sendtner, M. , Wiese, S. and Klausmeyer, A.
Lectin-Based Isolation and Culture of Mouse Embryonic Motoneurons. ,
J Vis Exp
 55 , e3200 , 2011
Cells
Motorneurons
Enzymes
Trypsin : 0.025%
Medium
HBSS
Neural
Mouse
Mouse, embryonic
Radad Khaled, Gille Gabriele, Rausch Wolf-Dieter.
Dopaminergic neurons are preferentially sensitive to long-term rotenone toxicity in primary cell culture ,
Toxicol In Vitro
 22 , 68-74 , 2008
Cells
Dopaminergic neurons
Enzymes
Trypsin : 0.1%
Medium
DMEM
Neural
Mouse
Mouse, neonatal
Pedrola, L. , Espert, A. , Valdes-Sanchez, T. , Sanchez-Piris, M. , Sirkowski, E. , Scherer, S. , Farinas, I. and Palau, F.
Cell Expression of GDAP1 in the Nervous System and Pathogenesis of Charcot-Marie-Tooth Type 4A Disease. ,
J Cell Mol Med
 12 , 679 , 2008
Cells
DRG neurons
Enzymes
Collagenase : 0.2%
Trypsin : 0.05%
Medium
Ham's F12
Neural
Mouse
Mouse
Panchision David M, Chen Hui-Ling, Pistollato Francesca, Papini Daniela, Ni Hsiao-Tzu, Hawley Teresa S.
Optimized flow cytometric analysis of central nervous system tissue reveals novel functional relationships among cells expressing CD133, CD15, and CD24 ,
Stem Cells
 25 , 1560-70 , 2007
Cells
Neural, various
Enzymes
Papain : 12 u/ml
Collagenase/Dispase : see reference
Trypsin : see reference
Medium
see reference
Neural
Mouse
Mouse, adult
Gonzalez John M, Bergmann Cornelia C, Fuss Babette, Hinton David R, Kangas Cindy, Macklin Wendy B, Stohlman Stephen A.
Expression of a dominant negative IFN-gammareceptor on mouse oligodendrocytes ,
Glia
 51 , 22-34 , 2005
Cells
Brain and spinal cord cells
Enzymes
Trypsin : 0.25%
Medium
PBS
Neural
Mouse
Mouse, 1-3 day
Savchenko V, Sung U, Blakely RD.
Cell surface trafficking of the antidepressant-sensitive norepinephrine transporter revealed with an ectodomain antibody ,
Mol Cell Neurosci
 24 , 1131 , 2003
Cells
Neurons, ganglia
Enzymes
Papain : 20 u/ml
Deoxyribonuclease I : 100 u/ml
Collagenase : 0.3%
Trypsin : 0.05%
Medium
HBSS
Neural
Mouse
Mouse, postnatal-day-1-old (P1)
Deshmukh, M. , Kuida, K. and Johnson Jr., E.
Caspase Inhibition Extends the Commitment to Neuronal Death Beyond Cytochrome c Release to the Point of Mitochondrial Depolarization ,
J Cell Biol
 150 (1) , 131 , 2000
Cells
Neurons
Enzymes
Trypsin : 0.25%
Medium
NGF-containing medium
Neural
Mouse
Mouse embryos
Lee, K. , Davies, A. and Jaenisch, R.
P75-Eficient Embryonic Dorsal Root Sensory and Neonatal Sympathetic Neurons Display a Decreased Sensitivity to NGF ,
Development
 120 , 1027 , 1994
Cells
Neurons, DRG and SCG
Enzymes
Trypsin : 0.25%
Medium
L-15 medium
Neural
Mouse
mouse, 65 days
Richards L. , Kilpatrick T. and Bartlett P.
De Novo Generation of Neuronal Cells from the Adult Mouse Brain ,
Proc Natl Acad Sci U S A
 89 , 8591 , 1992
Cells
Neurons, neuronal precursors
Enzymes
Trypsin : 0.1%
Medium
DMEM
Neural
Mouse
Mouse, CD-1, neonate
Quinn, S. and De Boni, U.
Enhanced Neuronal Regeneration by Retinoic Acid of Murine Dorsal Root Ganglia and of Fetal Murine and human Spinal Cord in vitro ,
In Vitro Cell Dev Biol
 27 , 55 , 1991
Cells
Neurons, dorsal root ganglion
Enzymes
Trypsin : 0.25%
Medium
HBSS
Neural
Mouse
Mouse, fetal
Kitani, H. , Shiurba, R. , Sakakura, T. and Tomooka, Y.
Isolation and Characterization Of Mouse Neural Precursor Cells in Primary Culture ,
In Vitro Cell Dev Biol
 27 , 615 , 1991
Cells
Precursor
Enzymes
Trypsin : 0.5%
Medium
PBS
Neural
Mouse
Mouse, neonatal
(also chick)
Varon, S. , Skaper, S. , Barbin, G. , Selak, I. and Manthorpe, M.
Low Molecular Weight Agents Support Survival of Cultured Neurons From the Central Nervous System ,
J Neurosci
 4 (3) , 654 , 1984
Cells
PNS test neurons
Enzymes
Trypsin : 0.08%
Medium
Eagle's Basal Medium (see reference)
Neural
Ovine
Lamb
(also calf)
Poduslo, S. , Miller, K. and McKhann, G.
Metabolic Properties of Maintained Oligodendroglia Purified from Brain ,
J Biol Chem
 253 , 1592 , 1978
Cells
Oligodendroglia
Neural
Enzymes
Trypsin : 0.1%
Medium
(see reference)
Neural
Quail
Quail
Sieber-Blum, M. and Cohen, A.
Clonal Analysis of Quail Neural Crest Cells: They are Pluripotent and Differentiate in Vitro in the Absence of Noncrest Cells ,
Dev Biol
 80 , 96 , 1980
Cells
Neural crest
Enzymes
Trypsin : 0.05%
Medium
MEM, HBSS
Neural
Rat
Rat, SD, day 2
Maurel, P.
Preparation of Neonatal Rat Schwann Cells and Embryonic Dorsal Root Ganglia Neurons for In Vitro Myelination Studies. ,
Methods Mol Biol
 1739 , 17-37 , 2018
Cells
Schwann
Enzymes
Trypsin : 0.25%
Medium
DMEM
Neural
Rat
Rat, embryonic 14.5 day
Mirsky, R. and Jessen, K.
Isolation of Schwann Cell Precursors from Rodents. ,
Methods Mol Biol
 1739 , 3-15 , 2018
Cells
Schwann cell precursors
Enzymes
Medium
DMEM/ Ham's F-12
Neural
Rat
Rat, SD, p7
Clements, M. , Byrne, E. , Camarillo Guerrero, L. , Cattin, A. , Zakka, L. , Ashraf, A. , Burden, J. , Khadayate, S. , Lloyd, A. , Marguerat, S. and Parrinello, S.
The Wound Microenvironment Reprograms Schwann Cells to Invasive Mesenchymal-like Cells to Drive Peripheral Nerve Regeneration. ,
Neuron
 96 , 98-114.e7 , 2017
Cells
Schwann
Enzymes
Medium
DMEM
Neural
Rat
Rat, SD
Andersen, N. , Srinivas, S. , Pinero, G. and Monje, P.
A Rapid and Versatile Method for the Isolation, Purification and Cryogenic Storage of Schwann Cells from Adult Rodent Nerves. ,
Sci Rep
 6 , 31781 , 2016
Cells
Schwann
Enzymes
Trypsin : 0.25%
Medium
DMEM
Neural
Rat
Rat, neonatal
Stettner, M. , Lohmann, B. , Wolffram, K. , Weinberger, J. , Dehmel, T. , Hartung, H. , Mausberg, A. and Kieseier, B.
Interleukin-17 Impedes Schwann Cell-Mediated Myelination. ,
J Neuroinflammation
 11 , 63 , 2014
Cells
Schwann cells
Enzymes
Collagenase : 0.05-0.1%
Trypsin : 0.125-0.25%
Medium
DMEM
Neural
Rat
Rat, SD, 0-2 day
Pedraza Carlos E, Podlesniy Petar, Vidal Noemi­, Arevalo Juan Carlos, Lee Ramee, Hempstead Barbara, Ferrer Isidre, Iglesias Montse, Espinet Carme.
Pro-NGF isolated from the human brain affected by Alzheimer's disease induces neuronal apoptosis mediated by p75NTR ,
Am J Pathol
 166 , 533-43 , 2005
Cells
Superior cervical ganglion
Enzymes
Collagenase Type 4 : 20 u/ml
Trypsin : 0.25%
Medium
DMEM
Neural
Rat
Rat, SD, male 150-220 g
Hu Hong-Zhen, Gu Qihai, Wang Chunbo, Colton Craig K, Tang Jisen, Kinoshita-Kawada Mariko, Lee Lu-Yuan, Wood Jackie D, Zhu Michael X.
2-aminoethoxydiphenyl borate is a common activator of TRPV1, TRPV2, and TRPV3 ,
J Biol Chem
 279 , 35741-8 , 2004
Cells
Dorsal root ganglion neurons
Enzymes
Trypsin : 0.05%
Medium
DMEM/Ham's F12
Neural
Rat
Rat, SD, embryonic
Bixby, S. , Kruger, G. , Mosher, J. , Joseph, N. and Morrison, S.
Cell-Intrinsic Differences Between Stem Cells from Different Regions of the Peripheral Nervous System Regulate the Generation of Neural Diversity. ,
Neuron
 35 , 643 , 2002
Cells
Sciatic nerve and gut neural crest stem
Enzymes
Trypsin : 0.005%
Medium
HBSS
Neural
Rat
Rat, newborn, 7 days old
Acosta, C. , Fabrega, A. , Masco, D. and Lopez, H.
A Sensory Neuron Subpopulation with Unique Sequential Survival Dependence on Nerve Growth Factor and Basic Fibroblast Growth Factor during Development ,
J Neurosci
 21 (22) , 8873 , 2001
Cells
Neurons
Enzymes
Trypsin : 0.25%
Medium
MEM10
Neural
Rat
Rat
(also mice)
Johansson, C. , Momma, S. , Clarke, D. , Risling, M. , Lendahl, U. and Frisen, J
Identification of a Neural Stem Cell in the Adult Mammalian Central Nervous System ,
Cell
 96 , 25 , 1999
Cells
Spinal cord
Enzymes
Trypsin : 0.133%
Medium
HBSS and PIPES
Neural
Rat
Rat, SD, female, pregnant
Morrison, S. , White, P. , Zock, C. and Anderson, D.
Prospective Identification, Isolation by Flow Cytometry, and In Vivo Self-Renewal of Multipotent Mammalian Neural Crest Stem Cells ,
Cell
 96 , 737 , 1999
Cells
Sciatic nerves
Enzymes
Trypsin : 0.025%
Medium
L-15 medium
(see reference)
Neural
Rat
Rat, SD, embryonic
Morrison, S. , White, P. , Zock, C. and Anderson, D.
Prospective Identification, Isolation by Flow Cytometry, and In Vivo Self-Renewal of Multipotent Mammalian Neural Crest Stem Cells ,
Cell
 96 , 737 , 1999
Cells
Sciatic nerves
Enzymes
Trypsin : 0.025%
Medium
L-15 medium
(see reference)
Neural
Rat
Rat, Wister and SD, newborn, 0-21 days, either sex
Schafer, K. , Saffrey, M. , Burnstock, G. and Mastres-Ventura, P.
A New Method for the Isolation of Myenteric Plexus from the Newborn Rat Gastrointestinal Tract ,
Brain Res Proto
 1 , 109 , 1997
Cells
Myenteric ganglia
Enzymes
Trypsin : 0.05%
Medium
MEM-HEPES
Neural
Rat
Rats, SD, 11-14 day old, 29-32 g
Allen, T. , Sim, J. and Brown, D.
The Whole-Cell Calcium Current in Acutely Dissociated Magnocellular Cholinergic Basal Forebrain Neurones of the Rat ,
J Physiol
 460 , 91 , 1993
Cells
Basal forebrain neurons
Enzymes
Trypsin : 0.125%
Medium
Gey's BSS
Neural
Rat
Rat, Wistar-Hanover, 7-12 day
Rayport, S. , Sulzer, D. , Shi, W. , Sawasdikosol, S. , Monaco, J. , Batson, D. and Rajendran, G.
Identified Postnatal Mesolimbic Dopamine Neurons in Culture: Morphology and Electrophysiology ,
J Neurosci
 12 (11) , 4264 , 1992
Cells
Postnatal dopamine neurons
Enzymes
Trypsin : 0.035%
Medium
(see reference)

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.