Tissue References Search Tool

Human

Human, 25-81 yr

Volovitz, I, Shapira, N. , Ezer, H. , Gafni, A. , Lustgarten, M. , Alter, T. , Ben-Horin, I. , Barzilai, O. , Shahar, T. , Kanner, A. , Fried, I. , Veshchev, I. , Grossman, R. and Ram, Z,

A Non-Aggressive, Highly Efficient, Enzymatic Method for Dissociation of Human Brain-Tumors and Brain-Tissues to Viable Single-Cells. ,
BMC Neurosci
17 , 30 , 2016

Cells

Brain tumor

Enzymes

Neutral Protease : 0.11 u/ml

Collagenase Type 4 : 0.05%

Hyaluronidase : 1,000 u/ml

Deoxyribonuclease I : 5 u/ml

Medium

HBSS

Human

Hussein, D. , Punjaruk, W. , Storer, L. , Shaw, L. , Othman, R. , Ottoman, R. , Peet, A. , Miller, S. , Bandopadhyay, G. , Heath, R. , Kumari, R. , Bowman, K. , Braker, P. , Rahman, R. , Jones, G. , Watson, S. and Lowe, J.

Pediatric Brain Tumor Cancer Stem Cells: Cell Cycle Dynamics, DNA Repair, and Etoposide Extrusion. ,
Neuro Oncol
13 , 70-83 , 2011

Cells

Brain tumor

Enzymes

Collagenase Type 1 : 0.04%

Hyaluronidase : 0.01%

Deoxyribonuclease I : 0.02%

Neutral Protease : 0.008%

Medium

DMEM/F12

Human

Human

Sauvageot, C. , Weatherbee, J. , Kesari, S. , Winters, S. , Barnes, J. , Dellagatta, J. , Ramakrishna, N. , Stiles, C. , Kung, A. , Kieran, M. and Wen, P.

Efficacy of the HSP90 Inhibitor 17-AAG in Human Glioma Cell Lines and Tumorigenic Glioma Stem Cells. , Neuro Oncol Vol. 11 , 109 , 2009

Cells

Tumor

Enzymes

Collagenase Type 4 : 0.1%

Hyaluronidase : 0.07%

Deoxyribonuclease I : 0.04%

Medium

see reference

Canine

Canine

Tongtako, W. , Lehmbecker, A. , Wang, Y. , Hahn, K. , Baumgartner, W. and Gerhauser, I.

Canine Dorsal Root Ganglia Satellite Glial Cells Represent an Exceptional Cell Population with Astrocytic and Oligodendrocytic Properties. ,
Sci Rep
7 , 13915 , 2017

Cells

Satellite glial

Enzymes

Hyaluronidase : 0.2%

Collagenase Type 4 : 0.2%

Collagenase : 0.2%

Medium

HBSS

Chicken

Chick, White Leghorn, embryos, 7 day

Wiseman, L. and Hammond, W.

The Reacquisition of Cell Adhesiveness Following Tissue Disaggregation by Eleven Different Agents ,
J Exp Zool
197 , 429 , 1976

Cells

Neural retina

Enzymes

Collagenase : 0.25%

Elastase : 0.2%

Hyaluronidase : 1.0%

Papain : 1.0%

Protease : 0.1%

Trypsin : 0.05%

Medium

HBSS

Mouse

Mouse, embryonic 12.5

Mirsky, R. and Jessen, K.

Isolation of Schwann Cell Precursors from Rodents. ,
Methods Mol Biol
1739 , 3-15 , 2018

Cells

Schwann cell precursors

Enzymes

Collagenase Type 2 : 0.2%

Hyaluronidase : 0.12%

Soybean Trypsin Inhibitor : 0.03%

Medium

DMEM/ Ham's F-12

Mouse

Mouse

Xu, W. , Sachewsky, N. , Azimi, A. , Hung, M. , Gappasov, A. and Morshead, C.

Myelin Basic Protein Regulates Primitive and Definitive Neural Stem Cell Proliferation from the Adult Spinal Cord. ,
Stem Cells
35 , 485-496 , 2017

Cells

Neurospheres

Enzymes

Trypsin : 0.13%

Hyaluronidase : 0.08%

Medium

Neurobasal A

Mouse

Mouse, 4 month

Tongtako, W. , Lehmbecker, A. , Wang, Y. , Hahn, K. , Baumgartner, W. and Gerhauser, I.

Canine Dorsal Root Ganglia Satellite Glial Cells Represent an Exceptional Cell Population with Astrocytic and Oligodendrocytic Properties. ,
Sci Rep
7 , 13915 , 2017

Cells

Satellite glial

Enzymes

Hyaluronidase : 0.2%

Collagenase Type 4 : 0.2%

Collagenase : 0.2%

Medium

HBSS

Mouse

Mouse, 6-8 week

Babona-Pilipos, R. , Popovic, M. and Morshead, C.

A Galvanotaxis Assay for Analysis of Neural Precursor Cell Migration Kinetics in an Externally Applied Direct Current Electric Field. ,
J Vis Exp
68 , e4193 , 2012

Cells

Neural precursors

Enzymes

Trypsin : 0.13%

Hyaluronidase : 0.08%

Medium

see reference

Rat

Rat, embryonic 14.5 day

Mirsky, R. and Jessen, K.

Isolation of Schwann Cell Precursors from Rodents. ,
Methods Mol Biol
1739 , 3-15 , 2018

Cells

Schwann cell precursors

Enzymes

Collagenase Type 2 : 0.2%

Hyaluronidase : 0.12%

Soybean Trypsin Inhibitor : 0.03%

Medium

DMEM/ Ham's F-12

Tissue Tables (references, grouped by tissue type and species)

Thyroid/Parathyroid