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Tissue References Search Tool

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Adrenal
Mouse
Mouse, 8-10 week
Kolski-Andreaco, A. , Cai, H. , Currle, D. , Chandy, K. and Chow, R.
Mouse Adrenal Chromaffin Cell Isolation. ,
J Vis Exp
 2 , 129 , 2007
Cells
Chromaffin
Enzymes
Papain : 40 u/ml
Medium
DMEM
Adrenal
Mouse
Mouse, embryonic
Tian Jin-Hua, Wu Zheng-Xing, Unzicker Michael, Lu Li, Cai Qian, Li Cuiling, Schirra Claudia, Matti Ulf, Stevens David, Deng Chuxia, Rettig Jens, Sheng Zu-Hang.
The role of Snapin in neurosecretion: snapin knock-out mice exhibit impaired calcium-dependent exocytosis of large dense-core vesicles in chromaffin cells ,
J Neurosci
 25 , 10546-55 , 2005
Cells
Chromafin cells
Enzymes
Papain : 20-25 u/ml
Medium
DMEM
Bone
Mouse
Mouse
Mohrin, D. , Koukos, G. , Alabdulaaly, L. , Paw, J. , Hake, K. , Kolumam, G. , Bouxsein, M. , Baron, R. , Kutskova, Y. , Freund, A. , Liu, J. , Zavala-Solorio, J. , Bhargava, S. , Maxwell, Trumble, J. , Brito, A. , Hu, D. and Brooks, D.
Inhibition of Longevity Regulator PAPP-A Modulates Tissue Homeostasis via Restraint of Mesenchymal Stromal Cells. , Aging Cell Vol. 20 , e13313 , 2021
Cells
Bone and bone marrow stromal
Enzymes
Medium
PBS
Bone
Mouse
Mouse
Baryawno, N. , Przybylski, D. , Kowalczyk, M. , Kfoury, Y. , Severe, N. , Gustafsson, K. , Kokkaliaris, K. , Mercier, F. , Tabaka, M. , Hofree, M. , Dionne, D. , Papazian, A. , Lee, D. , Ashenberg, O. and Subramanian, A.
A Cellular Taxonomy of the Bone Marrow Stroma in Homeostasis and Leukemia. ,
Cell
 177 , 1915-1932.e16 , 2019
Cells
Bone and bone marrow stroma
Enzymes
Medium
Media 199
Bone
Mouse
Mouse
Lee, D. , Choung, H. , Kim, H. , Gronostajski, R. , Yang, Y. , Ryoo, H. , Lee, Z. , Kim, H. , Cho, E. and Park, J.
NFI-C Regulates Osteoblast Differentiation Via Control of Osterix Expression. ,
Stem Cells
 32 , 2467-79 , 2014
Cells
Osteoblasts
Enzymes
Medium
MEM
Brain
Mouse
Adult and neonatal
Calvo, Belen, Rubio, F. , Fernandez, M. and Tranque, P.
Dissociation of Neonatal and Adult Mice Brain for Simultaneous Analysis of Microglia, Astrocytes and Infiltrating Lymphocytes by Flow Cytometry. , IBRO Rep Vol. 8 , 36-47 , 2020
Cells
Microglia, astrocytes, lymphocytes
Enzymes
Collagenase Type 2 : 120 u/ml
Papain : 20 u/ml
Neutral Protease : 1.2 u/ml
Medium
EBSS
Brain
Mouse
Mouse
Kramer, D. , Risso, D. , Kosillo,. , Ngai, J. and Bateup, H.
Combinatorial Expression of Grp and Neurod6 Defines Dopamine Neuron Populations with Distinct Projection Patterns and Disease Vulnerability , eNeuro Vol. 5 , 1 , 2018
Cells
Neurons
Enzymes
Papain : 10 u/ml
Medium
Artificial CSF
Brain
Mouse
Mouse, adult
Swartzlander, D. , Propson, N. , Roy, E. , Saito, T. , Saido, T. , Wang, B. and Zheng, H.
Concurrent Cell Type-Specific Isolation and Profiling of Mouse Brains in Inflammation and Alzheimer's Disease. ,
JCI Insight
 3 , 2018
Cells
Astrocytes, microglia, endothelia, oligodendrocytes
Enzymes
Papain : 20 u/ml
Medium
HBSS
Brain
Mouse
Mouse, fetal and mature
Zhang, Y. , Sloan, S. , Clarke, L. , Caneda, C. , Plaza, C. , Blumenthal, P. , Vogel, H. , Steinberg, G. , Edwards, M. , Li, G. , Duncan, J. , Cheshier, S. , Shuer, L. , Chang, E. , Grant, G. , Gephart, M. and Barres, B.
Purification and Characterization of Progenitor and Mature Human Astrocytes Reveals Transcriptional and Functional Differences with Mouse. ,
Neuron
 89 , 37-53 , 2016
Cells
Astrocytes and neurons
Enzymes
Papain : 7.5-20 u/ml
Medium
RPMI
Brain
Mouse
Mouse, 3 week
Luo,Y.,Coskun, V. , Liang, A.,Yu, J. , Cheng, L.,Ge, W. , Shi, Z. , Zhang, K. , Li, C. , Lin, H. , Luo, D. , Wang, J. , Lin, C. , Dai, Z. , Zhu, H. , Zhang, J. , Liu, J. , Liu, H.,deVellis, J. , Horvath, S. , Sun, Y. and Li, S.
Different Patterns of Molecular Forms of Somatostatin are Released by the Rat Median Eminence and Hypothalamus. ,
Cell
 161 , 1175 , 2015
Cells
Neural
Enzymes
Papain : 20 u/ml
Medium
PBS
Brain
Mouse
Mouse, P13-15
Haseleu, J. , Anlauf, E. , Blaess, S. , Endl, E. and Derouiche, A.
Studying Subcellular Detail in Fixed Astrocytes: Dissociation of Morphologically Intact Glial Cells (DIMIGs). ,
Front Cell Neurosci
 7 , 54 , 2013
Cells
Glial
Enzymes
Papain : 20 u/ml
Medium
EBSS
Brain
Mouse
Mouse, P0-P15
Foo, L.
Purification of Rat and Mouse Astrocytes by Immunopanning. ,
Cold Spring Harb. Protoc.
 2013 , 421-32 , 2013
Cells
Astrocytes
Enzymes
Papain : 5 u/ml
Medium
DPBS
Brain
Mouse
Mouse, 1-2 day
O'Meara, R. , Ryan, S. , Colognato, H. and Kothary, R.
Derivation of Enriched Oligodendrocyte Cultures and Oligodendrocyte/Neuron Myelinating Co-Cultures from Post-Natal Murine Tissues. ,
J Vis Exp
 54 , 3324 , 2011
Cells
Oligodendrocytes, dorsal root ganglia
Enzymes
Papain : 0.15%
Medium
DMEM
Brain
Mouse
Mouse, 6-12 week
Chung, W. , Farley, J. , Swenson, A. , Barnard, J. , Hamilton, G. , Chiposi, R. and Drummond, H.
Extracellular Acidosis Activates ASIC-like Channels in Freshly Isolated Cerebral Artery Smooth Muscle Cells. ,
Am J Physiol Cell Physiol
 298 , C1198 , 2010
Cells
Vascular smooth muscle cells
Enzymes
Papain : 0.05%
Elastase : 0.05%
Medium
PBS
Brain
Mouse
Mouse, neonatal
Fasano, C. , Thibault, D. and Trudeau, L.
Culture of Postnatal Mesencephalic Dopamine Neurons on an Astrocyte Monolayer , Current Protocols in Neuroscience Vol. 44 , 3.21.1 , 2008
Cells
Neurons
Enzymes
Papain : 20 u/ml
Medium
Neurobasal
Brain
Mouse
Mouse, embryonic and postnatal
Hilgenberg, L. and Smith, M.
Preparation of Dissociated Mouse Cortical Neuron Cultures. ,
J Vis Exp
 10 , 562 , 2007
Cells
Cortical neurons
Enzymes
Papain : 4-10 u/ml
Medium
Neurobasal
Brain
Mouse
Mouse
Oliver, T. , Read, T. , Kessler, J. , Mehmeti, A. , Wells, J. , Huynh, T. , Lin, S. and Wechsler-Reya, R.
Loss of Patched and Disruption of Granule Cell Development in a Pre-Neoplastic Stage of Medulloblastoma. ,
Development
 132 , 2425 , 2005
Cells
Granule cell precursors, pre-neoplastic and tumor cells
Enzymes
Papain : 10 u/ml
Deoxyribonuclease I : 250 u/ml
Medium
Neurobasal/B27
Brain
Mouse
Mouse, 3 day
Nishioku T, Hashimoto K, Yamashita K, Liou SY, Kagamiishi Y, Maegawa H, Katsube N, Peters C, von Figura K, Saftig P, Katunuma N, Yamamoto K, Nakanishi H.
Involvement of cathepsin E in exogenous antigen processing in primary cultured murine microglia ,
J Biol Chem
 277 , 4816 , 2002
Cells
Microglia
Enzymes
Papain : 90 u/ml
Deoxyribonuclease I : 2000 u/ml
Medium
Eagle's MEM
Brain
Mouse
Mouse
Miyazawa K, Himi T, Garcia V, Yamagishi H, Sato S. and Ishizaki Y.
A role for p27/Kip1 in the control of cerebellar granule cell precursor proliferation ,
J Neurosci
 20 , 5756 , 2000
Cells
Cerebellar granule cell precursors
Enzymes
Papain : 0.435%
Medium
EBSS
Brain
Mouse
Mouse, newborn
Jun K, Choi G, Yang SG, Choi KY, Kim H, Chan GC, Storm DR, Albert C, Mayr GW, Lee CJ, Shin HS.
Enhanced hippocampal CA1 LTP but normal spatial learning in inositol 1,4,5-trisphosphate 3-kinase(A)-deficient mice ,
Learn Mem
 5 , 317-30 , 1998
Cells
Hippocampal cells
Enzymes
Papain : 10 u/ml
Medium
DMKM
Colon
Mouse
Mouse
Fattahi, F. , Steinbeck, J. , Kriks, S. , Tchieu, J. , Zimmer, B. , Kishinevsky, S. , Zeltner, N. , Mica, Y. , El-Nachef, W. , Zhao, H. , de Stanchina, E. , Gershon, M. , Grikscheit, T. , Chen, S. and Studer, L.
Deriving Human ENS Lineages for Cell Therapy and Drug Discovery in Hirschsprung Disease. ,
Nature
 531 , 105-9 , 2016
Cells
Colon organoid
Enzymes
Collagenase Type 1 : 800 u/ml
Neutral Protease : 0.013%
Medium
DMEM
Colon
Mouse
Mouse, 8-10 week
Round, J. and Mazmanian, S.
Inducible Foxp3+ Regulatory T-cell Development by a Commensal Bacterium of the Intestinal Microbiota. ,
Proc Natl Acad Sci U S A
 107 , 12204-9 , 2010
Cells
Lamina propria lymphocytes
Enzymes
Medium
HBSS
Colon
Mouse
Mouse
Atarashi, K. , Nishimura, J. , Shima, T. , Umesaki, Y. , Yamamoto, M. , Onoue, M. , Yagita, H. , Ishii, N. , Evans, R. , Honda, K. and Takeda, K.
ATP Drives Lamina Propria T(H)17 Cell Differentiation. ,
Nature
 455 , 808 , 2008
Cells
Lamina propria
Enzymes
Medium
RPMI 1640
Colon
Mouse
Mouse
Weigmann Benno, Tubbe Ingrid, Seidel Daniel, Nicolaev Alex, Becker Christoph, Neurath Markus F.
Isolation and subsequent analysis of murine lamina propria mononuclear cells from colonic tissue ,
Nat Protoc
 2 , 2307-11 , 2007
Cells
Lamina propria mononuclear cells
Enzymes
Medium
HBSS
Endothelial
Mouse
Mouse
Luo, S. , Truong, A. and Makino, A.
Isolation of Mouse Coronary Endothelial Cells. ,
J Vis Exp
 , 2016
Cells
Coronary endothelial
Enzymes
Medium
Kreb's
Endothelial
Mouse
Mouse
Xiao, L. , McCann, J. and Dudley, A.
Isolation and Culture Expansion of Tumor-specific Endothelial Cells. ,
J Vis Exp
 , e53072 , 2015
Cells
Endothelial
Enzymes
Medium
DMEM
Endothelial
Mouse
Mouse
Kochukov, M. , Balasubramanian, A. , Abramowitz, J. , Birnbaumer, L. and Marrelli, S.
Activation of Endothelial Transient Receptor Potential C3 Channel is Required for Small Conductance Calcium-Activated Potassium Channel Activation and Sustained Endothelial Hyperpolarization and Vasodilation of Cerebral Artery. ,
J Am Heart Assoc
 3 , 2014
Cells
Cerebral artery endothelial
Enzymes
Neutral Protease : 4 u/ml
Elastase : 1 u/ml
Collagenase Type 1 : 120 u/ml
Medium
DMEM
Endothelial
Mouse
Mouse
Daneman, R. , Zhou, L. , Agalliu, D. , Cahoy, J. , Kaushal, A. and Barres, B.
The Mouse Blood-Brain Barrier Transcriptome: a New Resource for Understanding the Development and Function of Brain Endothelial Cells. ,
PLoS ONE
 5 , e13741 , 2010
Cells
Endothelial
Enzymes
Papain : 40 u/ml
Deoxyribonuclease I : 125 u/ml
Medium
DPBS
Endothelial
Mouse
Mouse, neonatal
Cha, S. , Talavera, D. , Demir, E. , Nath, A. and Sierra-Honigmann, M.
A Method of Isolation and Culture of Microvascular Endothelial Cells from Mouse Skin. ,
Microvasc Res
 70 , 198 , 2005
Cells
Microvascular endothelial
Enzymes
Medium
DMEM
Epithelial
Mouse
Mouse, 8-12 week
Brann, D. , Tsukahara, T. , Weinreb, C. , Lipovsek, M. , Van den Berge, K. , Gong, B. , Chance, R. , Macaulay, I. , Chou, H. , Fletcher, R. , Das, D. , Street, K. , de Bezieux, H. , Choi, Y. , Risso, D. and Dudoit, S.
Non-Neuronal Expression of SARS-CoV-2 Entry Genes in the Olfactory System Suggests Mechanisms Underlying COVID-19-Associated Anosmia. ,
Sci. Adv.
 6 , 2020
Cells
Nasal epithelium
Enzymes
Papain : 20 u/ml
Medium
EBSS
Epithelial
Mouse
Mouse
Sun, F. , Xiao, G. and Qu, Z.
Isolation of Murine Alveolar Type II Epithelial Cells. ,
Bio-protocol
 7 , 2017
Cells
Alveolar epithelial
Enzymes
Medium
DMEM
Epithelial
Mouse
Mouse
Pardo-Saganta, A. , Tata, P. , Law, B. , Saez, B. , Chow, R. , Prabhu, M. , Gridley, T. and Rajagopal, J.
Parent Stem Cells Can Serve as Niches for Their Daughter Cells. ,
Nature
 523 , 597-601 , 2015
Cells
Tracheal epithelial
Enzymes
Papain : 50 u/ml
Medium
See reference
Epithelial
Mouse
Mouse
Mathew, R. , Degenhard,t K. , Haramaty, L. , Karp, C. and White, E.
Immortalized Mouse Epithelial Cell Models to Study the Role of Apoptosis in Cancer. ,
Methods Enzymol
 446 , 77-106 , 2008
Cells
Epithelial
Enzymes
Collagenase : 0.025%
Medium
DMEM
Eye
Mouse

Mouse, 2 month

Murenu, E. , Pavlou, M. , Richter, L. , Rapti, K. , Just, S. and Cehajic-Kapetanovic, J.
A Universal Protocol for Isolating Retinal ON Bipolar Cells Across Species via Fluorescence-Activated Cell Sorting. , Mol Ther Methods Clin Dev Vol. 20 , 587-600 , 2021
Enzymes
Papain : 20 u/ml
Medium
EBSS
Eye
Mouse
Mouse
Ortin-Martinez, A. , Tsai, E. , Nickerson, P. , Bergeret, M. , Lu, Y. , Smiley, S. , Comanita, L. and Wallace, V.
A Reinterpretation of Cell Transplantation: GFP Transfer From Donor to Host Photoreceptors. ,
Stem Cells
 35 , 932-939 , 2017
Cells
Retinal
Enzymes
Papain : 20 u/ml
Medium
PBS
Eye
Mouse
Mouse
Wohl, S. , Jorstad, N. , Levine, E. and Reh, T.
Muller Glial microRNAs are Required for the Maintenance of Glial Homeostasis and Retinal Architecture. ,
Nat Commun
 8 , 1603 , 2017
Cells
Retinal
Enzymes
Papain : 20 u/ml
Medium
HBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.