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Tissue References Search Tool

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Adipose/Fat
Rat
Rat, SD, neonatal
Liu, Z. , Wang, H. , Zhang, Y. , Zhou, J. , Lin, Q. , Wang, Y. , Duan, C. , Wu, K. and Wang, C.
Efficient Isolation of Cardiac Stem Cells from Brown Adipose. ,
J Biomed Biotechnol
 2010 , 104296 , 2010
Cells
Brown adipocytes
Enzymes
Medium
PBS
Adipose/Fat
Rat
Rat, SD, various weights and ages
Woodward, Julie A. and Saggerson, E.
Effect of Adenosine Deaminase, N6-Phenylisopropyladenosine and Hypothroidism on the Responsiveness of Rat Brown Adipocytes to Noradrenaline ,
Biochem J
 238 , 395 , 1986
Cells
Brown adipocytes
Interscapular & cervical depots
Enzymes
Medium
Krebs Ringer bicarbonate buffer
Brain
Rat
Rat, SD, one month old
Bowman, P. , Betz, A. , Ar, D. , Wolinsky, J. , Penney, J. , Shivers, R. and Goldstein, G.
Primary Culture of Capillary Endothelium From Rat Brain ,
In Vitro
 17 (4) , 353 , 1981
Cells
Capillary endothelium
Pericytes
Enzymes
Medium
Medium 199
Colon
Rat
Rat, adult
Somara, S. , Bashllari, D. , Gilmont, R. and Bitar, K.
Real-Time Dynamic Movement of Caveolin-1 During Smooth Muscle Contraction of Human Colon and Aged Rat Colon Transfected with Caveolin-1 cDNA. ,
Am J Physiol Gastrointest Liver Physiol
 300 , G1022 , 2011
Cells
Colon smooth muscle
Enzymes
Medium
DMEM
Endothelial
Rat
Rat, SD, male, 250-300 g
Schwertschlag, U.S. and Whorton, A.R.
Platelet-Activating Factor-Induced Homologous and Heterologous Desensitization In Cultured Vascular Smooth Muscle Cells ,
J Biol Chem
 263 , 13791 , 1988
Cells
Smooth muscle, aorta
Enzymes
Medium
HBSS with 0.2 mM Ca++
Epithelial
Rat
Rat, embryonic
Shannon JM, Gebb SA. and Nielsen LD
Induction of alveolar type II cell differentiation in embryonic tracheal epithelium in mesenchyme-free culture ,
Development
 126 , 1675 , 1999
Cells
Tracheal epithelial
Enzymes
Medium
DMEM/F12
Epithelial
Rat
Rat, 6 day old
Evans GS, Flint N, Somers AS, Eyden B. and Potten CS.
The development of a method for the preparation of rat intestinal epithelial cell primary cultures ,
J Cell Sci
 101 , 219 , 1992
Cells
Rat intestinal epithelial
Enzymes
Collagenase : 300 u/ml
Medium
DMEM
Epithelial
Rat
Rat, 6-8 day
Chang CW, Roque RS, Defoe DM. and Caldwell RB.
An improved method for isolation and culture of pigment epithelial cells from rat retina ,
Curr Eye Res
 10(11) , 1081 , 1991
Cells
Retinal pigment epithelial
Enzymes
Medium
DMEM
Eye
Rat
Rat, 6-8 day
Chang CW, Roque RS, Defoe DM. and Caldwell RB.
An improved method for isolation and culture of pigment epithelial cells from rat retina ,
Curr Eye Res
 10(11) , 1081 , 1991
Cells
Retinal pigment epithelial
Enzymes
Medium
DMEM
Liver
Rat
Rat, Fisher
Blouin, M. and Marceau, N.
Primary Culture of Fetal Rat Liver Bipotential Progenitor Cells ,
J Tiss Cul Meth
 13 , 117 , 1991
Cells
Epithelial
Enzymes
Collagenase : 0.05%
Hyaluronidase : 0.075%
Medium
Eagles's MEM
Mammary
Rat
Rat, SD
Brake P. , Zhang L. and Jefcoate C.
Aryl Hydrocarbon Receptor Regulation of Cytochrome P4501B1 in Rat Mammary Fibroblasts: Evidence for Transcriptional Repression by Glucocorticoids ,
Mol Pharmacol
 54 , 825 , 1998
Cells
Mammary fibroblasts
Enzymes
Medium
DMEM/F-12
Mammary
Rat
Rat, SD, 50 day
Varela L. , Darcy K. and Ip M.
The Epidermal Growth Factor Receptor is not Required for Tumor Necrosis Factor-alpha Action in Normal Mammary Epithelial Cells ,
Endocrinology
 138 , 3891 , 1997
Cells
Mammary epithelial
Enzymes
Medium
DMEM/F12
Mammary
Rat
Rat, SD, female, 55 day
Ehmann, U. , Osborn, R. , Guzman, R. and Fajardo, L.
Cultured Proliferating Rat Mammary Epithelial Cells ,
In Vitro Cell Dev Biol
 27 , 749 , 1991
Cells
Epithelial
Enzymes
Neutral Protease : 3 u/ml
Medium
Medium 199
Mammary
Rat
Rat, 50-60 days
Hahm, H.A. and Ip, M.M.
Primary Culture of Normal Rat Mammary Epithelial Cells Within a Basement Matrix. 1. Regulation of Proliferation by Hormones and Growth Factors ,
In Vitro Cell Dev Biol
 26 , 791 , 1990
Cells
Epithelial
Enzymes
Medium
EBSS
Miscellaneous
Rat
Rat, SD, 6-8 month
Zhou, B. , Zhou, Y. and Tang, K.
The Effects of Substance P on Pluripotent Tendon Cells: an In Vitro and In Vivo Study. ,
J Musculoskelet Neuronal Interact
 14 , 349-58 , 2014
Cells
Pluripotent tendon
Enzymes
Medium
DMEM
Muscle
Rat
Rat, day 7
Vilmont, V. , Cadot, B. , Ouanoumou, G. and Gomes, E.
A System for Studying Mechanisms of Neuromuscular Junction Development and Maintence ,
Development
 143 , 2464 , 2016
Cells
Skeletal muscle
Enzymes
Collagenase : 0.05%
Medium
DMEM
Muscle
Rat
Rat
Weber, S. , Gratopp, A. , Akanbi, S. , Rheinlaender, C. , Sallmon, H. , Barikbin, P. and Koehne, P.
Isolation and Culture of Fibroblasts, Vascular Smooth Muscle, and Endothelial Cells From the Fetal Rat Ductus Arteriosus ,
Pediatr Res
 70 , 236 , 2011
Cells
Vascular smooth muscle
Enzymes
Medium
DMEM
Muscle
Rat
Rat
Pacak, C. and Cowan, D.
Fabrication of Myogenic Engineered Tissue Constructs. ,
J Vis Exp
 27 , e1137 , 2009
Cells
Myogenic
Enzymes
Medium
Ham's F-10
Muscle
Rat
Rat
Larkin, L. , Calve, S. , Kostrominova, T. and Arruda, E.
Structure and Functional Evaluation of Tendon-Skeletal Muscle Constructs Engineered In Vitro. ,
Tiss Eng
 12 , 3149-58 , 2006
Cells
myogenic
Enzymes
Neutral Protease : 8 u/ml
Collagenase Type 4 : 200 u/ml
Medium
DMEM
Muscle
Rat
Rat, Lewis, neonatal
Kim, J. , Hadlock, T. , Cheney, M. , Varvares, M. and Marler, J.
Muscle Tissue Engineering for Partial Glossectomy Defects. ,
Arch Facial Plast Surg
 5 , 403 , 2003
Cells
Myoblasts
Enzymes
Medium
Ham's F-10
Muscle
Rat
Rat, SD, adult and neonatal w/in 1 day of birth
Dennis, R. , Kosnik II, P. , Gilbert, M. and Faulkner, J.
Excitability and Contractility of Skeletal Muscle Engineered from Primary Cultures and Cell Lines ,
Am J Physiol Cell Physiol
 280 , C288 , 2001
Cells
Myooids
Enzymes
Neutral Protease : 4 u/ml
Medium
Ham's F-12
Muscle
Rat
Rat, SD, 3 month
Stevenson S. , Rollence M. , Marshall-Neff J. and Liau G.
A Genetically Modified Adenoviral Vector Exhibits Enhanced Gene Transfer of Human Smooth Muscle Cells ,
J Vasc Res
 38(5) , 471 , 2001
Cells
Smooth muscle cells
Enzymes
Medium
M-199
Muscle
Rat
Rat, SD, 225-250g
Gunther S, Alexander RW, Atkinson WJ. and Gimbrone MA Jr.
Functional angiotensin II receptors in cultured vascular smooth muscle cells ,
J Cell Biol
 92 , 289 , 1982
Cells
Mesenteric artery smooth muscle cells
Enzymes
Medium
HBSS
Neural
Rat
Rat, SD, 3 month
Andersen, N. and Monje, P.
Isolation, Culture, and Cryopreservation of Adult Rodent Schwann Cells Derived from Immediately Dissociated Teased Fibers. ,
Methods Mol Biol
 1739 , 49-66 , 2018
Cells
Schwann
Enzymes
Medium
DMEM
Neural
Rat
Rat, embryonic 14.5 day
Mirsky, R. and Jessen, K.
Isolation of Schwann Cell Precursors from Rodents. ,
Methods Mol Biol
 1739 , 3-15 , 2018
Cells
Schwann cell precursors
Enzymes
Medium
DMEM/ Ham's F-12
Neural
Rat
Rat, SD
Andersen, N. , Srinivas, S. , Pinero, G. and Monje, P.
A Rapid and Versatile Method for the Isolation, Purification and Cryogenic Storage of Schwann Cells from Adult Rodent Nerves. ,
Sci Rep
 6 , 31781 , 2016
Cells
Schwann
Enzymes
Trypsin : 0.25%
Medium
DMEM
Neural
Rat
Rat, E19-21 and P1-3
Ghogha, A. , Bruun, D. and Lein, P.
Inducing Dendritic Growth in Cultured Sympathetic Neurons. ,
J Vis Exp
 61 , e3546 , 2012
Cells
Sympathetic neurons
Enzymes
Medium
DMEM/F-12
Neural
Rat
Rat, 20-30 day
Bosmans, F. , Puopolo, M. , Martin-Eauclaire, M. , Bean, B. and Swartz, K.
Functional Properties and Toxin Pharmacology of a Dorsal Root Ganglion Sodium Channel Viewed through its Voltage Sensors. ,
J Gen Physiol
 138 , 59 , 2011
Cells
Dorsal root ganglion
Enzymes
Papain : 20 u/ml
Medium
L-15
Neural
Rat
Rat, Fisher, 8-9 week
Lie DC, Dziewczapolski G, Willhoite AR, Kaspar BK, Shults CW, Gage FH.
The adult substantia nigra contains progenitor cells with neurogenic potential ,
J Neurosci
 22 , 6639 , 2002
Cells
Adult progenitor
Enzymes
Papain : 2.5 u/ml
Deoxyribonuclease I : 250 u/ml
Neutral Protease : 1 u/ml
Medium
DMEM/F-12
Neural
Rat
Rat, SD, adult, 250-300g, P8
Davies, S. , Fitch, M. , Memberg, S. , Hall, A, Raisman, G. and Silver, J.
Regeneration of Adult Axons in White Matter Tracts of the Central Nervous System ,
Nature
 390 , 680 , 1997
Cells
Neurons, DRG
Enzymes
Medium
L-15 w/ CO2
Neural
Rat
Rat, 1-6 day
Robertson, S. , Rae, M. , Rowan, E. and Kennedy, C.
Characterization of a P2X-Purinoceptor in Cultured Neurones of the Rat Dorsal Root Ganglia. ,
Br J Pharmacol
 118 , 951 , 1996
Cells
DRG neurons
Enzymes
Papain : 20 u/ml
Collagenase Type 1 : 150 u/ml
Medium
HBSS
Neural
Rat
Rat, pups, 14 day old
McFarlane, S. and Cooper, E.
Extrinsic Factors Influence the Expression of Voltage-Gated K Currents on Neonatal Rat Sympathetic Neurons ,
J Neurosci
 13 (6) , 2591 , 1993
Cells
Neurons, sympathetic
Enzymes
Medium
HBSS
Pancreas
Rat
Rat, SD, adult
Li, J. , Zhou, R. , Bie, B. , Huang, N. , Guo, Y. , Chen, H. , Shi, M. , Yang, J. , Zhang, J. and Li, Z.
Emodin and Baicalein Inhibit Sodium Taurocholate-Induced Vacuole Formation in Pancreatic Acinar Cells. ,
World J Gastroenterol
 24 , 35-45 , 2018
Cells
Acinar
Enzymes
Medium
Culture media
Pancreas
Rat
Rat, SD, male
Ji B, Kopin AS, Logsdon CD.
Species differences between rat and mouse CCKA receptors determine the divergent acinar cell response to the cholecystokinin analog JMV-180 ,
J Biol Chem
 275 , 19115-20 , 2000
Cells
Ancinar
Enzymes
CLSPA : see reference
Medium
DMEM
Pancreas
Rat
Rat
Blinman TA, Gukovsky I, Mouria M, Zaninovic V, Livingston E, Pandol SJ, Gukovskaya AS.
Activation of pancreatic acinar cells on isolation from tissue: cytokine upregulation via p38 MAP kinase ,
Am J Physiol Cell Physiol
 279 , C1993-2003 , 2000
Cells
Pancreatic acini
Enzymes
CLSPA : 30 u/ml
Collagenase Type 4 : 30 u/ml
Medium
M199
Pancreas
Rat
Rat, SD, male, 150 - 200 g
Menozzi, D. , Sato, S. , Jensen, R. and Gardner, J.
Cyclic GMP Does Not Inhibit Protein Kinase C-Mediated Enzyme Secretion in Rat Pancreatic Acini ,
J Biol Chem
 264 , 995 , 1989
Cells
Acinar
Enzymes
Medium
HEPES

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.