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Tissue References Search Tool

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Tissue

Species

Enzyme(s)

Neural
Mouse
Mouse, postnatal-day-1-old (P1)
Deshmukh, M. , Kuida, K. and Johnson Jr., E.
Caspase Inhibition Extends the Commitment to Neuronal Death Beyond Cytochrome c Release to the Point of Mitochondrial Depolarization ,
J Cell Biol
 150 (1) , 131 , 2000
Cells
Neurons
Enzymes
Trypsin : 0.25%
Medium
NGF-containing medium
Neural
Mouse
Mouse embryos
Lee, K. , Davies, A. and Jaenisch, R.
P75-Eficient Embryonic Dorsal Root Sensory and Neonatal Sympathetic Neurons Display a Decreased Sensitivity to NGF ,
Development
 120 , 1027 , 1994
Cells
Neurons, DRG and SCG
Enzymes
Trypsin : 0.25%
Medium
L-15 medium
Neural
Mouse
mouse, 65 days
Richards L. , Kilpatrick T. and Bartlett P.
De Novo Generation of Neuronal Cells from the Adult Mouse Brain ,
Proc Natl Acad Sci U S A
 89 , 8591 , 1992
Cells
Neurons, neuronal precursors
Enzymes
Trypsin : 0.1%
Medium
DMEM
Neural
Mouse
Mouse, CD-1, neonate
Quinn, S. and De Boni, U.
Enhanced Neuronal Regeneration by Retinoic Acid of Murine Dorsal Root Ganglia and of Fetal Murine and human Spinal Cord in vitro ,
In Vitro Cell Dev Biol
 27 , 55 , 1991
Cells
Neurons, dorsal root ganglion
Enzymes
Trypsin : 0.25%
Medium
HBSS
Neural
Mouse
Mouse, fetal
Kitani, H. , Shiurba, R. , Sakakura, T. and Tomooka, Y.
Isolation and Characterization Of Mouse Neural Precursor Cells in Primary Culture ,
In Vitro Cell Dev Biol
 27 , 615 , 1991
Cells
Precursor
Enzymes
Trypsin : 0.5%
Medium
PBS
Neural
Mouse
Mouse, neonatal
(also chick)
Varon, S. , Skaper, S. , Barbin, G. , Selak, I. and Manthorpe, M.
Low Molecular Weight Agents Support Survival of Cultured Neurons From the Central Nervous System ,
J Neurosci
 4 (3) , 654 , 1984
Cells
PNS test neurons
Enzymes
Trypsin : 0.08%
Medium
Eagle's Basal Medium (see reference)
Neural
Ovine
Lamb
(also calf)
Poduslo, S. , Miller, K. and McKhann, G.
Metabolic Properties of Maintained Oligodendroglia Purified from Brain ,
J Biol Chem
 253 , 1592 , 1978
Cells
Oligodendroglia
Neural
Enzymes
Trypsin : 0.1%
Medium
(see reference)
Neural
Porcine
Porcine, adult, 60-100 kg
Si ML, Lee TJ.
Presynaptic alpha7-nicotinic acetylcholine receptors mediate nicotine-induced nitric oxidergic neurogenic vasodilation in porcine basilar arteries ,
J Pharmacol Exp Ther
 298 , 122 , 2001
Cells
Superior cervical ganglia
Enzymes
Papain : 2 u/ml
Collagenase : 0.12%
Medium
HBSS
Neural
Quail
Quail
Sieber-Blum, M. and Cohen, A.
Clonal Analysis of Quail Neural Crest Cells: They are Pluripotent and Differentiate in Vitro in the Absence of Noncrest Cells ,
Dev Biol
 80 , 96 , 1980
Cells
Neural crest
Enzymes
Trypsin : 0.05%
Medium
MEM, HBSS
Neural
Rat
Rat, SD, 3 month
Andersen, N. and Monje, P.
Isolation, Culture, and Cryopreservation of Adult Rodent Schwann Cells Derived from Immediately Dissociated Teased Fibers. ,
Methods Mol Biol
 1739 , 49-66 , 2018
Cells
Schwann
Enzymes
Medium
DMEM
File
11612.pdf (854.59 KB)
Neural
Rat
Rat, SD, day 2
Maurel, P.
Preparation of Neonatal Rat Schwann Cells and Embryonic Dorsal Root Ganglia Neurons for In Vitro Myelination Studies. ,
Methods Mol Biol
 1739 , 17-37 , 2018
Cells
Schwann
Enzymes
Trypsin : 0.25%
Medium
DMEM
File
11615.pdf (837.77 KB)
Neural
Rat
Rat, SD, p7
Clements, M. , Byrne, E. , Camarillo Guerrero, L. , Cattin, A. , Zakka, L. , Ashraf, A. , Burden, J. , Khadayate, S. , Lloyd, A. , Marguerat, S. and Parrinello, S.
The Wound Microenvironment Reprograms Schwann Cells to Invasive Mesenchymal-like Cells to Drive Peripheral Nerve Regeneration. ,
Neuron
 96 , 98-114.e7 , 2017
Cells
Schwann
Enzymes
Medium
DMEM
File
11677.pdf (7.17 MB)
Neural
Rat
Rat, SD
Andersen, N. , Srinivas, S. , Pinero, G. and Monje, P.
A Rapid and Versatile Method for the Isolation, Purification and Cryogenic Storage of Schwann Cells from Adult Rodent Nerves. ,
Sci Rep
 6 , 31781 , 2016
Cells
Schwann
Enzymes
Trypsin : 0.25%
Medium
DMEM
File
11697.pdf (3.48 MB)
Neural
Rat
Rat, neonatal
Stettner, M. , Lohmann, B. , Wolffram, K. , Weinberger, J. , Dehmel, T. , Hartung, H. , Mausberg, A. and Kieseier, B.
Interleukin-17 Impedes Schwann Cell-Mediated Myelination. ,
J Neuroinflammation
 11 , 63 , 2014
Cells
Schwann cells
Enzymes
Collagenase : 0.05-0.1%
Trypsin : 0.125-0.25%
Medium
DMEM
File
11081.pdf (1.58 MB)
Neural
Rat
Rat, E19-21 and P1-3
Ghogha, A. , Bruun, D. and Lein, P.
Inducing Dendritic Growth in Cultured Sympathetic Neurons. ,
J Vis Exp
 61 , e3546 , 2012
Cells
Sympathetic neurons
Enzymes
Medium
DMEM/F-12
File
10909.pdf (315.05 KB)
Neural
Rat
Rat, 20-30 day
Bosmans, F. , Puopolo, M. , Martin-Eauclaire, M. , Bean, B. and Swartz, K.
Functional Properties and Toxin Pharmacology of a Dorsal Root Ganglion Sodium Channel Viewed through its Voltage Sensors. ,
J Gen Physiol
 138 , 59 , 2011
Cells
Dorsal root ganglion
Enzymes
Papain : 20 u/ml
Medium
L-15
File
10869.pdf (2.29 MB)
Neural
Rat
Rat, SD, 0-2 day
Pedraza Carlos E, Podlesniy Petar, Vidal Noemi­, Arevalo Juan Carlos, Lee Ramee, Hempstead Barbara, Ferrer Isidre, Iglesias Montse, Espinet Carme.
Pro-NGF isolated from the human brain affected by Alzheimer's disease induces neuronal apoptosis mediated by p75NTR ,
Am J Pathol
 166 , 533-43 , 2005
Cells
Superior cervical ganglion
Enzymes
Collagenase Type 4 : 20 u/ml
Trypsin : 0.25%
Medium
DMEM
Neural
Rat
Rat, SD, male 150-220 g
Hu Hong-Zhen, Gu Qihai, Wang Chunbo, Colton Craig K, Tang Jisen, Kinoshita-Kawada Mariko, Lee Lu-Yuan, Wood Jackie D, Zhu Michael X.
2-aminoethoxydiphenyl borate is a common activator of TRPV1, TRPV2, and TRPV3 ,
J Biol Chem
 279 , 35741-8 , 2004
Cells
Dorsal root ganglion neurons
Enzymes
Trypsin : 0.05%
Medium
DMEM/Ham's F12
Neural
Rat
Rat, Fisher, 8-9 week
Lie DC, Dziewczapolski G, Willhoite AR, Kaspar BK, Shults CW, Gage FH.
The adult substantia nigra contains progenitor cells with neurogenic potential ,
J Neurosci
 22 , 6639 , 2002
Cells
Adult progenitor
Enzymes
Papain : 2.5 u/ml
Deoxyribonuclease I : 250 u/ml
Neutral Protease : 1 u/ml
Medium
DMEM/F-12
Neural
Rat
Rat, SD, embryonic
Bixby, S. , Kruger, G. , Mosher, J. , Joseph, N. and Morrison, S.
Cell-Intrinsic Differences Between Stem Cells from Different Regions of the Peripheral Nervous System Regulate the Generation of Neural Diversity. ,
Neuron
 35 , 643 , 2002
Cells
Sciatic nerve and gut neural crest stem
Enzymes
Trypsin : 0.005%
Medium
HBSS
File
10890.pdf (834.28 KB)
Neural
Rat
Rat, newborn, 7 days old
Acosta, C. , Fabrega, A. , Masco, D. and Lopez, H.
A Sensory Neuron Subpopulation with Unique Sequential Survival Dependence on Nerve Growth Factor and Basic Fibroblast Growth Factor during Development ,
J Neurosci
 21 (22) , 8873 , 2001
Cells
Neurons
Enzymes
Trypsin : 0.25%
Medium
MEM10
Neural
Rat
Rat
(also mice)
Johansson, C. , Momma, S. , Clarke, D. , Risling, M. , Lendahl, U. and Frisen, J
Identification of a Neural Stem Cell in the Adult Mammalian Central Nervous System ,
Cell
 96 , 25 , 1999
Cells
Spinal cord
Enzymes
Trypsin : 0.133%
Medium
HBSS and PIPES
Neural
Rat
Rat, SD, female, pregnant
Morrison, S. , White, P. , Zock, C. and Anderson, D.
Prospective Identification, Isolation by Flow Cytometry, and In Vivo Self-Renewal of Multipotent Mammalian Neural Crest Stem Cells ,
Cell
 96 , 737 , 1999
Cells
Sciatic nerves
Enzymes
Trypsin : 0.025%
Medium
L-15 medium
(see reference)
File
1099.pdf (350.54 KB)
Neural
Rat
Rat, SD, embryonic
Morrison, S. , White, P. , Zock, C. and Anderson, D.
Prospective Identification, Isolation by Flow Cytometry, and In Vivo Self-Renewal of Multipotent Mammalian Neural Crest Stem Cells ,
Cell
 96 , 737 , 1999
Cells
Sciatic nerves
Enzymes
Trypsin : 0.025%
Medium
L-15 medium
(see reference)
File
1099.pdf (350.54 KB)
Neural
Rat
Rat, Wister and SD, newborn, 0-21 days, either sex
Schafer, K. , Saffrey, M. , Burnstock, G. and Mastres-Ventura, P.
A New Method for the Isolation of Myenteric Plexus from the Newborn Rat Gastrointestinal Tract ,
Brain Res Proto
 1 , 109 , 1997
Cells
Myenteric ganglia
Enzymes
Trypsin : 0.05%
Medium
MEM-HEPES
Neural
Rat
Rat, SD, adult, 250-300g, P8
Davies, S. , Fitch, M. , Memberg, S. , Hall, A, Raisman, G. and Silver, J.
Regeneration of Adult Axons in White Matter Tracts of the Central Nervous System ,
Nature
 390 , 680 , 1997
Cells
Neurons, DRG
Enzymes
Medium
L-15 w/ CO2
Neural
Rat
Rat, 1-6 day
Robertson, S. , Rae, M. , Rowan, E. and Kennedy, C.
Characterization of a P2X-Purinoceptor in Cultured Neurones of the Rat Dorsal Root Ganglia. ,
Br J Pharmacol
 118 , 951 , 1996
Cells
DRG neurons
Enzymes
Papain : 20 u/ml
Collagenase Type 1 : 150 u/ml
Medium
HBSS
File
10618.pdf (2.97 MB)
Neural
Rat
Rat, pups, 14 day old
McFarlane, S. and Cooper, E.
Extrinsic Factors Influence the Expression of Voltage-Gated K Currents on Neonatal Rat Sympathetic Neurons ,
J Neurosci
 13 (6) , 2591 , 1993
Cells
Neurons, sympathetic
Enzymes
Medium
HBSS
Neural
Rat
Rats, SD, 11-14 day old, 29-32 g
Allen, T. , Sim, J. and Brown, D.
The Whole-Cell Calcium Current in Acutely Dissociated Magnocellular Cholinergic Basal Forebrain Neurones of the Rat ,
J Physiol
 460 , 91 , 1993
Cells
Basal forebrain neurons
Enzymes
Trypsin : 0.125%
Medium
Gey's BSS
Neural
Rat
Rat, Wistar-Hanover, 7-12 day
Rayport, S. , Sulzer, D. , Shi, W. , Sawasdikosol, S. , Monaco, J. , Batson, D. and Rajendran, G.
Identified Postnatal Mesolimbic Dopamine Neurons in Culture: Morphology and Electrophysiology ,
J Neurosci
 12 (11) , 4264 , 1992
Cells
Postnatal dopamine neurons
Enzymes
Trypsin : 0.035%
Medium
(see reference)
Neural
Rat
Rat, SD, 18-day-old, fetus
Cheng, B. and Mattson, M.
IGF-I and IGF-II Protect Cultured Hippocampal and Septal Neurons Against Calcium-Mediated Hypoglycemic Damage ,
J Neurosci
 12 (4) , 1558 , 1992
Cells
Hippocampal
Enzymes
Trypsin : 0.2%
Medium
HBSS
Neural
Rat
Rat, SD, pregnant, Charles River
Buchhalter, J. and Dichter, M.
Electrophysiological Comparison of Pyramidal and Stellate Nonpyramidal Neurons in Dissociated Cell Culture of Rat Hippocampus ,
Brain Res Bull
 26 , 333 , 1991
Cells
Pyramidal neurons
Nonpyramidal neurons
Enzymes
Trypsin : 0.027%
Medium
HEPES
Neural
Rat
Rat, SD, embryos, 19-21 days gestation
Mithen, F. , Reiker, M. and Birchem, R.
Effects of Ethanol on Rat Schwann Cell Proliferation and Myelination in Culture ,
In Vitro Cell Dev Biol
 26 , 129 , 1990
Cells
Schwann, dorsal root ganglia
Enzymes
Trypsin : 0.25%
Medium
HBSS, CMF
Neural
Rat
Rat
(also bovine)
Trifaro, J. , Tang, R. and Novas, M.
Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons ,
In Vitro Cell Dev Biol
 26 , 335 , 1990
Cells
Heart
Adrenal chromaffin
Paraneurons
Enzymes
Trypsin : 0.06%
Medium
25mM HEPES buffered Locke's solution, CMF
Neural
Rat
Rat, SD, 8 day old pups
Novelli, A. , Reilly, J. , Lysko, P. and Henneberry, R.
Glutamate Becomes Neurotoxic Via the N-methyl-D-aspartate Receptor When Intracellular Energy Levels are Reduced ,
Brain Res
 451 , 205 , 1988
Cells
Cerebellar neurons
Enzymes
Trypsin : 0.025%
Medium
Eagle's MEM
Neural
Rat
Rat, fetuses, 18 day old
Mattson, M. and Kater, S.
Isolated Hippocampal Neurons in Cryopreserved Long-Term Cultures: Development of Neuroarchitecture and Sensitivity to Nmda ,
Int J Dev Neurosci
 6 (5) , 439 , 1988
Cells
Hippocampal neurons
Enzymes
Trypsin : 0.2%
Medium
Eagle's MEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.