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Tissue References Search Tool

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Adipose/Fat
Human
Human
Esteve, D. , Boulet, N. , Volat, F. , Zakaroff-Girard, A. , Ledoux, S. , Coupaye, M. , Decaunes, P. , Belles, C. , Gaits-Iacovoni, F. , Iacovoni, J. , Remaury, A. , Castel, B. , Ferrara, P. , Heymes, C. and Lafonta, M. Boloumie, J.
Human White and Brite Adipogenesis is Supported by MSCA1 and is impaired by Immune Cells. ,
Stem Cells
 33 , 1277-91 , 2015
Cells
Adipose stroma-vascular
Enzymes
Neutral Protease : 2.4 u/ml
Collagenase : 250 u/ml
Medium
PBS
Adipose/Fat
Human
Human
Nie, J. , Chang, B. , Traktuev, D. , Sun, J. , March, K. , Chan, L. , Sage, H. , Pasqualini, R. , Arap, W. and Kolonin, M.
IFATS Collection: Combinatorial Peptides Identify alpha-5 beta-1 Integrin as a Receptor for the Matricellular Protein SPARC on Adipose Stromal Cells. ,
Stem Cells
 26 , 2735 , 2008
Cells
Stromal
Enzymes
Medium
DMEM
Adipose/Fat
Rat
Rat, SD, neonatal
Liu, Z. , Wang, H. , Zhang, Y. , Zhou, J. , Lin, Q. , Wang, Y. , Duan, C. , Wu, K. and Wang, C.
Efficient Isolation of Cardiac Stem Cells from Brown Adipose. ,
J Biomed Biotechnol
 2010 , 104296 , 2010
Cells
Brown adipocytes
Enzymes
Medium
PBS
Brain
Bovine
Bovine
Kanda, T. , Yoshino, H. , Ariga, T. , Yamawaki, M. and Yu, R.
Glycosphingolipid Antigens in Cultured Microvasular Bovine Brain Endothelial Cells: Sulfoglucuronosyl Paragloboside as a Target of Monoclonal IgM in Demyelinative Neuropathy ,
J Cell Biol
 126 (1) , 235 , 1994
Cells
Microvascular endothelial
Enzymes
Neutral Protease : 0.005%
Medium
Medium 199
Brain
Bovine
Bovine
Miller, D. , Audus, K. and Borchardt, R.
Application of Cultured Endothelial Cells of the Brain Microvasculature in the Study of the Blood-Brain Barrier ,
J Tiss Cul Meth
 14 , 217 , 1992
Cells
Endothelial
Enzymes
Neutral Protease : 0.125%
Medium
MEM
Brain
Bovine
Bovine
Audus, K. and Borchardt, R.
Characterization of an In Vitro Blood-Brain Barrier Model System for Studying Drug Transport and Metabolism ,
Pharm Res
 3 (2) , 81 , 1986
Cells
Endothelial
Enzymes
Medium
MEM
Brain
Human
Human
Rustenhoven, J. , Park Thomas, I. , Schweder, P. , Scotter, J. , Correia, J. , Smith, A. , Gibbons, H. , Oldfield, R. , Bergin, P. , Mee, E. , Faull, R. , Curtis, M. , Scott, G. and Dragunow, M.
Isolation of Highly Enriched Primary Human Microglia for Functional Studies. ,
Sci Rep
 6 , 19371 , 2016
Cells
Microglia
Enzymes
Neutral Protease : 10 u/ml
Papain : 2.5 u/ml
Medium
Hibernate A
Brain
Human
Human, 25-81 yr
Volovitz, I, Shapira, N. , Ezer, H. , Gafni, A. , Lustgarten, M. , Alter, T. , Ben-Horin, I. , Barzilai, O. , Shahar, T. , Kanner, A. , Fried, I. , Veshchev, I. , Grossman, R. and Ram, Z,
A Non-Aggressive, Highly Efficient, Enzymatic Method for Dissociation of Human Brain-Tumors and Brain-Tissues to Viable Single-Cells. ,
BMC Neurosci
 17 , 30 , 2016
Cells
Brain tumor
Enzymes
Neutral Protease : 0.11 u/ml
Hyaluronidase : 1,000 u/ml
Medium
HBSS
Brain
Human
Hussein, D. , Punjaruk, W. , Storer, L. , Shaw, L. , Othman, R. , Ottoman, R. , Peet, A. , Miller, S. , Bandopadhyay, G. , Heath, R. , Kumari, R. , Bowman, K. , Braker, P. , Rahman, R. , Jones, G. , Watson, S. and Lowe, J.
Pediatric Brain Tumor Cancer Stem Cells: Cell Cycle Dynamics, DNA Repair, and Etoposide Extrusion. ,
Neuro Oncol
 13 , 70-83 , 2011
Cells
Brain tumor
Enzymes
Medium
DMEM/F12
Brain
Mouse
Adult and neonatal
Calvo, Belen, Rubio, F. , Fernandez, M. and Tranque, P.
Dissociation of Neonatal and Adult Mice Brain for Simultaneous Analysis of Microglia, Astrocytes and Infiltrating Lymphocytes by Flow Cytometry. , IBRO Rep Vol. 8 , 36-47 , 2020
Cells
Microglia, astrocytes, lymphocytes
Enzymes
Collagenase Type 2 : 120 u/ml
Papain : 20 u/ml
Neutral Protease : 1.2 u/ml
Medium
EBSS
Brain
Rat
Rat, SD, one month old
Bowman, P. , Betz, A. , Ar, D. , Wolinsky, J. , Penney, J. , Shivers, R. and Goldstein, G.
Primary Culture of Capillary Endothelium From Rat Brain ,
In Vitro
 17 (4) , 353 , 1981
Cells
Capillary endothelium
Pericytes
Enzymes
Medium
Medium 199

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.