For our international customers, please be advised that orders cannot be placed through our website by customers in countries with International Distributor representation.

Quick Order

Tissue References Search Tool

Search
Brain
Bovine
Bovine
Kanda, T. , Yoshino, H. , Ariga, T. , Yamawaki, M. and Yu, R.
Glycosphingolipid Antigens in Cultured Microvasular Bovine Brain Endothelial Cells: Sulfoglucuronosyl Paragloboside as a Target of Monoclonal IgM in Demyelinative Neuropathy ,
J Cell Biol
 126 (1) , 235 , 1994
Cells
Microvascular endothelial
Enzymes
Neutral Protease : 0.005%
Medium
Medium 199
Brain
Bovine
Bovine
Miller, D. , Audus, K. and Borchardt, R.
Application of Cultured Endothelial Cells of the Brain Microvasculature in the Study of the Blood-Brain Barrier ,
J Tiss Cul Meth
 14 , 217 , 1992
Cells
Endothelial
Enzymes
Neutral Protease : 0.125%
Medium
MEM
Brain
Bovine
Bovine
Audus, K. and Borchardt, R.
Characterization of an In Vitro Blood-Brain Barrier Model System for Studying Drug Transport and Metabolism ,
Pharm Res
 3 (2) , 81 , 1986
Cells
Endothelial
Enzymes
Medium
MEM
Brain
Human
Human
Rustenhoven, J. , Park Thomas, I. , Schweder, P. , Scotter, J. , Correia, J. , Smith, A. , Gibbons, H. , Oldfield, R. , Bergin, P. , Mee, E. , Faull, R. , Curtis, M. , Scott, G. and Dragunow, M.
Isolation of Highly Enriched Primary Human Microglia for Functional Studies. ,
Sci Rep
 6 , 19371 , 2016
Cells
Microglia
Enzymes
Neutral Protease : 10 u/ml
Papain : 2.5 u/ml
Medium
Hibernate A
Brain
Human
Human, 25-81 yr
Volovitz, I, Shapira, N. , Ezer, H. , Gafni, A. , Lustgarten, M. , Alter, T. , Ben-Horin, I. , Barzilai, O. , Shahar, T. , Kanner, A. , Fried, I. , Veshchev, I. , Grossman, R. and Ram, Z,
A Non-Aggressive, Highly Efficient, Enzymatic Method for Dissociation of Human Brain-Tumors and Brain-Tissues to Viable Single-Cells. ,
BMC Neurosci
 17 , 30 , 2016
Cells
Brain tumor
Enzymes
Neutral Protease : 0.11 u/ml
Hyaluronidase : 1,000 u/ml
Medium
HBSS
Brain
Human
Hussein, D. , Punjaruk, W. , Storer, L. , Shaw, L. , Othman, R. , Ottoman, R. , Peet, A. , Miller, S. , Bandopadhyay, G. , Heath, R. , Kumari, R. , Bowman, K. , Braker, P. , Rahman, R. , Jones, G. , Watson, S. and Lowe, J.
Pediatric Brain Tumor Cancer Stem Cells: Cell Cycle Dynamics, DNA Repair, and Etoposide Extrusion. ,
Neuro Oncol
 13 , 70-83 , 2011
Cells
Brain tumor
Enzymes
Medium
DMEM/F12
Brain
Mouse
Adult and neonatal
Calvo, Belen, Rubio, F. , Fernandez, M. and Tranque, P.
Dissociation of Neonatal and Adult Mice Brain for Simultaneous Analysis of Microglia, Astrocytes and Infiltrating Lymphocytes by Flow Cytometry. , IBRO Rep Vol. 8 , 36-47 , 2020
Cells
Microglia, astrocytes, lymphocytes
Enzymes
Collagenase Type 2 : 120 u/ml
Papain : 20 u/ml
Neutral Protease : 1.2 u/ml
Medium
EBSS
Brain
Rat
Rat, SD, one month old
Bowman, P. , Betz, A. , Ar, D. , Wolinsky, J. , Penney, J. , Shivers, R. and Goldstein, G.
Primary Culture of Capillary Endothelium From Rat Brain ,
In Vitro
 17 (4) , 353 , 1981
Cells
Capillary endothelium
Pericytes
Enzymes
Medium
Medium 199
Mammary
Mouse

Mouse, female

Sun, H. , Xu, X. and Deng, C.
Preparation of Single Epithelial Cells Suspension from Mouse Mammary Glands. ,
Bio Protoc
 10 , e3530 , 2020
Cells
Mammary epithelial
Enzymes
Medium
DMEM/F-12
Mammary
Mouse
Mouse, 12 week
Taddei, I. , Deugnier, M. , Faraldo, M. , Petit, V. , Bouvard, D. , Medina, D. , Fassler, R. , Thiery, J. and Glukhova, M.
Beta1 Integrin Deletion from the Basal Compartment of the Mammary Epithelium Affects Stem Cells ,
Nat Cell Biol
 10 , 716-22 , 2008
Cells
Mammary epithelial
Enzymes
Collagenase : 0.3%
Hyaluronidase : 100 u/ml
Trypsin : 0.25%
Medium
see reference
Mammary
Rat
Rat, SD
Brake P. , Zhang L. and Jefcoate C.
Aryl Hydrocarbon Receptor Regulation of Cytochrome P4501B1 in Rat Mammary Fibroblasts: Evidence for Transcriptional Repression by Glucocorticoids ,
Mol Pharmacol
 54 , 825 , 1998
Cells
Mammary fibroblasts
Enzymes
Medium
DMEM/F-12
Mammary
Rat
Rat, SD, 50 day
Varela L. , Darcy K. and Ip M.
The Epidermal Growth Factor Receptor is not Required for Tumor Necrosis Factor-alpha Action in Normal Mammary Epithelial Cells ,
Endocrinology
 138 , 3891 , 1997
Cells
Mammary epithelial
Enzymes
Medium
DMEM/F12
Mammary
Rat
Rat, SD, female, 55 day
Ehmann, U. , Osborn, R. , Guzman, R. and Fajardo, L.
Cultured Proliferating Rat Mammary Epithelial Cells ,
In Vitro Cell Dev Biol
 27 , 749 , 1991
Cells
Epithelial
Enzymes
Neutral Protease : 3 u/ml
Medium
Medium 199
Mammary
Rat
Rat, 50-60 days
Hahm, H.A. and Ip, M.M.
Primary Culture of Normal Rat Mammary Epithelial Cells Within a Basement Matrix. 1. Regulation of Proliferation by Hormones and Growth Factors ,
In Vitro Cell Dev Biol
 26 , 791 , 1990
Cells
Epithelial
Enzymes
Medium
EBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.