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Tissue References Search Tool

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Brain
Mouse

Mouse, adult

Joseph, D. , Deimling, M. , Hasegawa, Y. , Cristancho, A. , Risbud, R. , McCoy, A. and Marsh, E.
Protocol for Isolating Young Adult Parvalbumin Interneurons from the Mouse Brain for Extraction of High-Quality RNA. , STAR Protoc Vol. 2 , 100714 , 2021
Cells
Interneurons
Medium
EBSS
Brain
Mouse
Mouse, adult
Saxena, A. , Wagatsuma, A. , Noro, Y. , Kuji, T. , Asaka-Oba, A. , Watahiki, A. , Gurnot, C. , Fagiolini, M. , Hensch, T. and Carninci, P.
Trehalose-Enhanced Isolation of Neuronal Sub-Types from Adult Mouse Brain. ,
Biotechniques
 52 , 381 , 2012
Cells
Neurons
Enzymes
PDS kit : with modifications
Medium
DMEM/F12
Brain
Mouse
Mouse, 4-6 day
Lee, H. , Greene, L. , Mason, C. and Manzini, M.
Isolation and Culture of Post-Natal Mouse Cerebellar Granule Neuron Progenitor Cells and Neurons. ,
J Vis Exp
 23 , 990 , 2009
Cells
Neurons
Enzymes
PDS kit : with modifications
Medium
HBSS
Brain
Mouse
Mouse, 1 day
Seaberg, R. , Smukler, S. and Van der Kooy, D.
Intrinsic Differences Distinguish Transiently Neurogenic Progenitors from Neural Stem Cells in the Early Postnatal Brain. ,
Dev Biol
 278 , 71 , 2005
Cells
Neural progenitor
Enzymes
PDS kit : per instructions
Medium
see reference
Brain
Mouse
Mouse
Klein C, Butt SJ, Machold RP, Johnson JE. and Fishell G.
Cerebellum- and forebrain-derived stem cells possess intrinsic regional character ,
Development
 132 , 4497 , 2005
Cells
Neurosperes
Enzymes
PDS kit : per instructions
Medium
DMEM/F12
Brain
Mouse
Mouse
Hernandez, F. , Perez, M. , Lucas, J. , Mata, A. , Bhat, R. and Avila, J.
Glycogen Synthase Kinase-3 plays a Crucial Role in Tau Exon 10 Splicing and Intranuclear Distribution of SC35. Implications for Alzheimer's Disease. ,
J Biol Chem
 279 , 3801 , 2004
Cells
Cortical neurons
Enzymes
PDS kit : per instructions
Medium
Neurobasal
Brain
Mouse
Mouse, 2-5 day
Smeyne Michelle, Smeyne RichardJ.
Method for culturing postnatal substantia nigra as an in vitro model of experimental Parkinson's disease ,
Brain Res Brain Res Protoc
 9 , 105-11 , 2002
Cells
Postnal substantia nigra
Enzymes
PDS kit : per instructions
Medium
see reference
Brain
Mouse
Mouse, embryonic
Estivill-Torrus, G. , Pearson, H. , Van Heyningen, V. , Price, D. and Rashbass, P.
Pax6 is Required to Regulate the Cell Cycle and the Rate of Progression from Symmetrical to Asymmetrical Division in Mammalian Cortical Progenitors. ,
Development
 129 , 455 , 2002
Cells
Cortical progenitors
Enzymes
PDS kit : per instructions
Medium
Serum free medium
Brain
Mouse
Mouse, 1 day
Martin-Aparicio, E. , Yamamoto, A. , Hernandez, F. , Hen, R. , Avila, J. and Lucas, J.
Proteasomal-Dependent Aggregate Reversal and Absence of Cell Death in a Conditional Mouse Model of Huntington's Disease. ,
J Neurosci
 21 , 8772 , 2001
Cells
Neurons and glia
Enzymes
PDS kit : per instructions
Medium
Neurobasal
Endothelial
Mouse
Mouse, 2 month
Sabbagh, M. and Nathans, J.
A Genome-wide View of the De-differentiation of Central Nervous System Endothelial Cells in Culture ,
Elife
 , 1 , 2020
Cells
Endothelial, CNS
Enzymes
PDS kit : with modifications
Stemxyme 1 : 0.1%
Medium
EC growth medium
Epithelial
Mouse
Mouse, adult
Gualdoni, S. , Baron, M. , Lakowski, J. , Decembrini, S. , Pearson, R. , Ali, R. and Sowden, J.
Isolation and Culture of Adult Ciliary Epithelial Cells, Previously Identified as Retinal Stem Cells, and Retinal Progenitor Cells. ,
Curr Protoc Stem Cell Biol
 Chapter 1 , Unit 1H.4 , 2011
Cells
Cilary epithelial
Enzymes
PDS kit : per instructions
Medium
EBSS
Epithelial
Mouse
Mouse, male, 8-16 week
Breggia, A. and Himmelfarb, J.
Primary Mouse Renal Tubular Epithelial Cells have Variable Injury Tolerance to Ischemic and Chemical Mediators of Oxidative Stress. ,
Oxid Med Cell Longev
 1 , 33 , 2008
Cells
Renal tubular epithelial
Enzymes
Collagenase : 200 u/ml
Soybean Trypsin Inhibitor : see reference
Medium
HBSS
Eye
Mouse
Mouse, 2-4 week
Liu, X. , Tang, L. and Liu Y.
Mouse Muller Cell Isolation and Culture. ,
Bio-protocol
 7 , 2017
Cells
Muller
Enzymes
PDS kit : per instructions
Medium
DMEM
Eye
Mouse
Mouse, adult
Feodorova, Y. , Koch, M. , Bultman, S. , Michalakis, S. and Solovei, I.
Quick and Reliable Method for Retina Dissociation and Separation of Rod Photoreceptor Perikarya from Adult Mice. ,
MethodsX
 2 , 39 , 2015
Cells
Retinal photoreceptor
Enzymes
PDS kit : with modifications
Medium
EBSS
Eye
Mouse
Mouse, 4-8 day
Balmer, J. , Zulliger, R. , Roberti, S. and Enzmann, V.
Retinal Cell Death Caused by Sodium Iodate Involves Multiple Caspase-Dependent and Caspase-Independent Cell-Death Pathways. ,
Int J Mol Sci
 16 , 15086-103 , 2015
Cells
Photoreceptors
Enzymes
PDS kit : per instructions
Medium
Neurobasal
Eye
Mouse
Mouse, 6 week
Singh, M. , Issa, P, Butler, R. , Martin, C. , Lipinski, D. , Sekaran, S. , Barnard, A. and Maclaren, R.
Reversal of End-Stage Retinal Degeneration and Restoration of Visual Bunction by Photoreceptor Transplantation. ,
Proc Natl Acad Sci U S A
 , 2013
Cells
Retinal
Enzymes
PDS kit : per instructions
Medium
DMEM
Eye
Mouse
Mouse, adult
Gualdoni, S. , Baron, M. , Lakowski, J. , Decembrini, S. , Pearson, R. , Ali, R. and Sowden, J.
Isolation and Culture of Adult Ciliary Epithelial Cells, Previously Identified as Retinal Stem Cells, and Retinal Progenitor Cells. ,
Curr Protoc Stem Cell Biol
 Chapter 1 , Unit 1H.4 , 2011
Cells
Cilary epithelial
Enzymes
PDS kit : per instructions
Medium
EBSS
Heart
Mouse
Mouse, 6-8 week
Qian, Q. , Hunter, L. , Li, M. , Marin-Padilla, M. , Prakash, Y. , Somlo, S. , Harris, P. , Torres, V. and Sieck, G.
Pkd2 Haploinsufficiency Alters Intracellular Calcium Regulation in Vascular Smooth Muscle Cells ,
Hum Mol Genet
 12(15) , 1875 , 2003
Cells
Vascular smooth muscle
Enzymes
Papain : 10 u/ml
Elastase : .005%
Collagenase : 0.05%
Deoxyribonuclease I : 1000 u/ml
PDS kit : with modifications
Medium
EBSS
Heart
Mouse
Mouse, 3-7 month
Bradshaw AD, Francki A, Motamed K, Howe C, Sage EH.
Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation ,
Mol Biol Cell
 10 , 1569-79 , 1999
Cells
Fibroblasts, mesangial, smooth muscle
Enzymes
Trypsin : 0.25%
Collagenase : see reference
Medium
DMEM
Kidney
Mouse

Mouse

Comeau, K. , Caillon, A. , Paradis, P. and Schiffrin, E.
Determination of Interleukin-17A and Interferon-γ Production in γδ, CD4, and CD8 T Cells Isolated from Murine Lymphoid Organs, Perivascular Adipose Tissue, Kidney, and Lung. ,
Bio Protoc
 13 , e4679 , 2023
Cells
T cells
Enzymes
Medium
RPMI
Kidney
Mouse
Mouse, male, 8-16 week
Breggia, A. and Himmelfarb, J.
Primary Mouse Renal Tubular Epithelial Cells have Variable Injury Tolerance to Ischemic and Chemical Mediators of Oxidative Stress. ,
Oxid Med Cell Longev
 1 , 33 , 2008
Cells
Renal tubular epithelial
Enzymes
Collagenase : 200 u/ml
Soybean Trypsin Inhibitor : see reference
Medium
HBSS
Kidney
Mouse
Mouse, male, 12-16 week
Cunningham, R. , Xiaofei, E. , Steplock, D. , Shenolikar, S. and Weinman, E.
Defective PTH Regulation of Sodium-Dependent Phosphate Transport in NHERF-1-/- Renal Proximal Tubule Cells and Wild-Type Cells Adapted to Low-Phosphate Media. ,
Am J Physiol Renal Physiol
 289 , F933 , 2005
Cells
Renal proximal tubule cells
Enzymes
Medium
DMEM/F-12
Kidney
Mouse
Mouse, 3-7 month
Bradshaw AD, Francki A, Motamed K, Howe C, Sage EH.
Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation ,
Mol Biol Cell
 10 , 1569-79 , 1999
Cells
Fibroblasts, mesangial, smooth muscle
Enzymes
Trypsin : 0.25%
Collagenase : see reference
Medium
DMEM
Lung
Mouse

Mouse

Comeau, K. , Caillon, A. , Paradis, P. and Schiffrin, E.
Determination of Interleukin-17A and Interferon-γ Production in γδ, CD4, and CD8 T Cells Isolated from Murine Lymphoid Organs, Perivascular Adipose Tissue, Kidney, and Lung. ,
Bio Protoc
 13 , e4679 , 2023
Cells
T cells
Enzymes
Medium
RPMI
Miscellaneous
Mouse
Mouse, 25-30 g
Minamoto Kanji, Pinsky DavidJ.
Recipient iNOS but not eNOS deficiency reduces luminal narrowing in tracheal allografts ,
J Exp Med
 196 , 1321-33 , 2002
Cells
Tracheal inflammatory cells
Enzymes
Medium
RPMI 1640
Neural
Mouse
Mouse, embryonic
Kramer, N. , Haney, M. , Morgens, D. , Jovicic, A. , Couthouis, J. , Li, A. , Ousey, J. , Ma, R. , Bieri, G. , Tsui, C. , Shi, Y. , Hertz, N. , Tessier-Lavigne, M. , Ichida, J. , Bassik, M. and Gitler, A.
CRISPR-Cas9 Screens in Human Cells and Primary Neurons Identify Modifiers of C9ORF72 Dipeptide-Repeat-Protein Toxicity. ,
Nat Genet
 , 2018
Cells
Cortical neurons
Enzymes
PDS kit : per instructions
Medium
Neurobasal
Neural
Mouse
Mouse, embryonic 12.5
Mirsky, R. and Jessen, K.
Isolation of Schwann Cell Precursors from Rodents. ,
Methods Mol Biol
 1739 , 3-15 , 2018
Cells
Schwann cell precursors
Enzymes
Medium
DMEM/ Ham's F-12
Neural
Mouse
Mouse
Ren, H. , Lu, T. , McGraw, T. and Accili, D.
Anorexia and Impaired Glucose Metabolism in Mice with Hypothalamic Ablation of Glut4 Neurons. ,
Diabetes
 64 , 405-17 , 2015
Cells
Neurons
Enzymes
PDS kit : per instructions
Medium
EBSS
Neural
Mouse
Mouse
Williams, E. , Zhong, X. , Mohamed, A. , Li, R. , Liu, Y. , Dong, Q. , Ananiev, G. , Mok, J. , Lin, B. , Lu, J. , Chiao, C. , Cherney, R. , Li, H. , Zhang, S. and Chang, Q
Mutant Astrocytes Differentiated from Rett Syndrome Patients-Specific iPSCs Have Adverse Effects on Wild-Type Neurons. ,
Hum Mol Genet
 23 , 2968-80 , 2014
Cells
Hippocampal neurons
Enzymes
PDS kit : per instructions
Medium
DMEM
Neural
Mouse
Mouse, neonatal
Gill JC, Moenter SM, Tsai PS.
Developmental regulation of gonadotropin-releasing hormone neurons by fibroblast growth factor signaling ,
Endocrinology
 145 , 3830 , 2004
Cells
Neurons
Enzymes
PDS kit : per instructions
Medium
EBSS
Pancreas
Mouse
Mouse, adult, male
Ji, B. , Gaiser, S. , Chen, X. , Ernst, S. and Logsdon, C.
Intracellular Trypsin Induces Pancreatic Acinar Cell Death but not NF-KappaB Activation. ,
J Biol Chem
 284 , 17488 , 2009
Cells
Ancinar
Enzymes
CLSPA : see reference
Medium
DMEM
Pancreas
Mouse
Mouse, 6-10 month
Wang, Y. , Soyombo, A. , Shcheynikov, N. , Zeng, W. , Dorwart, M. , Marino, C. , Thomas, P. and Muallem, S.
Slc26a6 Regulates CFTR Activity In Vivo to Determine Pancreatic Duct HCO3- Secretion: Relevance to Cystic Fibrosis. ,
EMBO J
 25 , 5049 , 2006
Cells
Pancreatic ductal
Enzymes
CLSPA : 50 u/ml
Hyaluronidase : 400 u/ml
Medium
DMEM
Pancreas
Mouse
Mouse, male, 18-24 g
Burnham DB, Williams JA.
Effects of carbachol, cholecystokinin, and insulin on protein phosphorylation in isolated pancreatic acini ,
J Biol Chem
 257 , 10523-8 , 1982
Cells
Acinar
Enzymes
CLSPA : 70-90 u/ml
Medium
Krebs-Henseleit
Pituitary
Mouse
Mouse
Pyczek, J. , Buslei, R. , Schult, D. , Holsken, A. , Buchfelder, M. , Hess, I. , Hahn, H. and Uhmann, A.
Hedgehog Signaling Activation Induces Stem Cell Proliferation and Hormone Release in the Adult Pituitary Gland. ,
Sci Rep
 6 , 24928 , 2016
Cells
Pituitary
Enzymes
Medium
DMEM
Skin
Mouse
Mouse, 3-7 month
Bradshaw AD, Francki A, Motamed K, Howe C, Sage EH.
Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation ,
Mol Biol Cell
 10 , 1569-79 , 1999
Cells
Fibroblasts, mesangial, smooth muscle
Enzymes
Trypsin : 0.25%
Collagenase : see reference
Medium
DMEM
Spleen
Mouse

Mouse

Comeau, K. , Caillon, A. , Paradis, P. and Schiffrin, E.
Determination of Interleukin-17A and Interferon-γ Production in γδ, CD4, and CD8 T Cells Isolated from Murine Lymphoid Organs, Perivascular Adipose Tissue, Kidney, and Lung. ,
Bio Protoc
 13 , e4679 , 2023
Cells
T cells
Enzymes
Medium
RPMI

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.