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Tissue References Search Tool

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Heart
Mouse
Mouse
Touchberry, C. , Green, T. , Tchikrizov, V. , Mannix, J. , Mao, T. , Carney, B. , Girgis, M. , Vincent, R. , Wetmore, L. , Dawn, B. , Bonewald, L. , Stubbs, J. and Wacker, M.
FGF23 is a Novel Regulator of Intracellular Calcium and Cardiac Contractility in Addition to Cardiac Hypertrophy. ,
Am J Physiol Endocrinol Metab
 304 , E863 , 2013
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 360 u/ml
Papain : 0.4 u/ml
Medium
L-15
Heart
Mouse
Mouse
Lu Tong, Ye Dan, Wang Xiaoli, Seubert John M, Graves Joan P, Bradbury J Alyce, Zeldin Darryl C, Lee Hon-Chi.
Cardiac and vascular KATP channels in rats are activated by endogenous epoxyeicosatrienoic acids through different mechanisms ,
J Physiol
 575 , 627-44 , 2006
Cells
Ventricular myocytes and mesenteric arterial SMC
Enzymes
Papain : 0.175%
Medium
Krebs-Ringer
Heart
Mouse
Mouse, 6-8 week
Qian, Q. , Hunter, L. , Li, M. , Marin-Padilla, M. , Prakash, Y. , Somlo, S. , Harris, P. , Torres, V. and Sieck, G.
Pkd2 Haploinsufficiency Alters Intracellular Calcium Regulation in Vascular Smooth Muscle Cells ,
Hum Mol Genet
 12(15) , 1875 , 2003
Cells
Vascular smooth muscle
Enzymes
Papain : 10 u/ml
Elastase : .005%
Collagenase : 0.05%
Deoxyribonuclease I : 1000 u/ml
PDS kit : with modifications
Medium
EBSS
Heart
Rat
Rat, SD, 200-250 g
Lu Tong, Ye Dan, Wang Xiaoli, Seubert John M, Graves Joan P, Bradbury J Alyce, Zeldin Darryl C, Lee Hon-Chi.
Cardiac and vascular KATP channels in rats are activated by endogenous epoxyeicosatrienoic acids through different mechanisms ,
J Physiol
 575 , 627-44 , 2006
Cells
Ventricular myocytes and mesenteric arterial SMC
Enzymes
Papain : 0.175%
Medium
Krebs-Ringer
Heart
Rat
Rat, SD, adult female
Puri S, Bansal DD, Uskokovic MR. and MacGregor RR.
Induction of tissue plasminogen activator secretion from rat heart microvascular cells by fM 1,25(OH)(2)D(3) ,
Am J Physiol/Endo
 278(2) , E293 , 2000
Cells
Heart microvascular cells
Enzymes
CLSPA : 250u/ml
Papain : 5 u/ml
Elastase : 0.8 u/ml
Medium
L-15
Stem
Human
Human
Rigamonti, A. , Repetti, G. , Sun, C. , Price, F. , Reny, D. , Rapino, F. , Weisinger, K. , Benkler, C. , Peterson, Q. , Davidow, L. , Hansson, E. and Rubin, L.
Large-Scale Production of Mature Neurons from Human Pluripotent Stem Cells in a Three-Dimensional Suspension Culture System. ,
Stem Cell Reports
 6 , 993-1008 , 2016
Cells
Motor neurons
Enzymes
Papain : 20 u/ml
Medium
EBSS
Stem
Human
Human
Welter, J. and Solchaga, L.and Penick, K.
Simplification of Aggregate Culture of Human Mesenchymal Stem Cells as a Chondrogenic Screening Assay ,
Biotechniques
 42 , 732 , 2007
Cells
Bone marrow derived MSC
Enzymes
Trypsin : 0.05%
Papain : 0.0025%
Medium
DMEM
Stem
Insect
Drosophila, larval
Berger, C. , Harzer, H. , Burkard, T. , Steinmann, J. , van der Horst, S. , Laurenson, A. , Novatchkova, M. , Reichert, H. and Knoblich, J.
FACS Purification and Transcriptome Analysis of Drosophila Neural Stem Cells Reveals a Role for Klumpfuss in Self-Renewal. ,
Cell Rep
 2 , 407 , 2012
Cells
Neuroblasts
Enzymes
Papain : 0.1%
Medium
Rinaldini solution
Stem
Mouse
Mouse, 6-8 week
Bracko, O. , Singer, T. , Aigner, S. , Knobloch, M. , Winner, B. , Ray, J. , Clemenson, G. , Suh, H. , Couillard-Despres, S. , Aigner, L. , Gage, F. and Jessberger, S.
Gene Expression Profiling of Neural Stem Cells and their Neuronal Progeny Reveals IGF2 as a Regulator of Adult Hippocampal Neurogenesis. ,
J Neurosci
 32 , 3376-87 , 2012
Cells
Neural stem cells
Enzymes
Medium
DMEM/F12
Stem
Mouse
Mouse, P4-5
Ziegler, A. , Schneider, J. , Qin, M. , Tyler, W. , Pintar, J. , Fraidenraich, D. , Wood, T. and Levison, S.
IGF-II Promotes Stemness of Neural Restricted Precursors. ,
Stem Cells
 30 , 1265 , 2012
Cells
Neuroshere
Enzymes
Trypsin : 0.25%
Papain : 100 u
Medium
Pro-N
Stem
Mouse
Mouse
Hutton, S. and Pevny, L.
Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous System. ,
Cold Spring Harb. Protoc.
 11 , 5077 , 2008
Cells
Neural progenitor cell
Enzymes
Papain : 10 ul/ml
Medium
PBS
Stem
Mouse
Mouse
Meletis, K. , Wirta, V. , Hede, S. , Nister, M. and Lundeberg, J. abd Frisen, J.
p53 Suppresses the Self-Renewal of Adult Neural Stem Cells. ,
Development
 133 , 363 , 2006
Cells
Neural stem cells
Enzymes
Papain : see reference
Medium
DMEM/F-12
Stem
Mouse
Mouse, day 7
Lee, A, Kessler, J, Read, T, Kaiser, C, Corbeil, D, Huttner ,W, Johnson, J, Wechsler-Reya, R.
Isolation of Neural Stem Cells from the Postnatal Cerebellum ,
Nat Neurosci
 8 , 723 , 2005
Cells
Cerebellar stem cells
Enzymes
Papain : 10u/ml
Deoxyribonuclease I : 250 u/ml
Medium
Dulbecco's PBS
Stem
Mouse
Mouse, 15 day or 3 month
Gritti, A, Bonfanti, L, Doetsch, F, Caille, I, Alvarez-Buylla, A, Lim, D, Galli, R, Verdugo J, Herrera, D. and Vescovi A.
Multipotent Neural Stem Cells Reside into the Rostral Extension and Olfactory Bulb of Adult Rodents ,
J Neurosci
 22(2) , 437 , 2002
Cells
Neural stem cells
Enzymes
Papain : 0.1%
Medium
DMEM/F-12
Stem
Rat
Rat, male, 200-250 g
Gobbel GT, Choi SJ, Beier S, Niranjan A.
Long-term cultivation of multipotential neural stem cells from adult rat subependyma ,
Brain Res
 980 , 221 , 2003
Cells
Neural stem cells
Enzymes
Papain : 0.09%
Medium
EBSS
Stem
Rat
Rat, adult
Palmer, T. , Markakis, E. , Willhoite, A. , Safar, F. and Gage, F.
Fibroblast Growth Factor-2 Activates a Latent Neurogenic Program in Neural Stem Cells from Diverse Regions of the Adult CNS ,
J Neurosci
 19 , 8487 , 1999
Cells
Neural stem cells
Enzymes
Papain : 2.5 u/ml
Deoxyribonuclease I : 250 u/ml
Neutral Protease : 1 u/ml
Medium
DMEM/F-12
Stem
Rat
Rat
Ray, J. and Gage, F.
Neural Stem Cell Isolation, Characterization and Transplantation , Modern Techniques in Neuroscience Research , Johansson, H. and Windhorst, U. , Springer-Verlag New York , 339 , 1999
Cells
Central nervous system stem cells
Enzymes
Papain : 0.01%
Medium
DMEM/F-12
Stem
Rat
Rat, embryonic
Maric, D. , Maric, I. and Barker, J.
Buoyant Density Gradient Fractionation and Flow Cytometric Analysis of Embryonic Rat Cortical Neurons and Progenitor Cells. ,
Methods
 16 , 247 , 1998
Cells
Neurons and progenitor
Enzymes
Papain : 20 u/ml
Medium
EBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.