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Tissue References Search Tool

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Eye
Chicken
Chick, embryo, 6 day
Jacob Vanessa, Rothermel Andrae, Wolf Peter, Layer Paul G.
Rhodopsin, violet and blue opsin expressions in the chick are highly dependent on tissue and serum conditions ,
Cell Death Differ
 180 , 159-68 , 2005
Cells
Retinal cells
Enzymes
Trypsin : 0.005%
Medium
DMEM/F12
Eye
Chicken
Chick embryo
Reenstra Wende R, Orlow Daniel L, Svoboda Kathy K H.
ECM-stimulated signaling and actin reorganization in embryonic corneal epithelia are Rho dependent ,
Inv Ophthal Visual Sci
 43 , 3181-9 , 2002
Cells
Corneal epithelia
Enzymes
Collagenase : 0.08%
Trypsin : 0.08%
Medium
HBSS
Eye
Chicken
Chick embryo
(also rat)
Seigel, G.
The Golden Age of Retinal Cell Culture ,
Mol Vis
 5 , 4 , 1999
Cells
Retinal
Enzymes
Trypsin : 0.6%
Medium
DMEM
Eye
Chicken
Chick, embryo, 10-14 day
Moyer, M. , Bullrich, F. and Sheffield, J.
Emergence of Flat Cells From Glia in Stationary Cultures of Embryonic Chick Neural Retina ,
In Vitro Cell Dev Biol
 26 , 1073 , 1990
Cells
Flat, retina
Enzymes
Trypsin : 0.1%
Medium
Tyrode's solution, CMF
Eye
Fish

Zebrafish, Danio rerio

Middel, C. , Dietrich, N. , Hammes, H. and Kroll, J.
Analysis of the Morphology of Retinal Vascular Cells in Zebrafish , Front Cell Dev Biol Vol. 11 , 1267232 , 2023
Cells
Retinal vascular
Enzymes
Trypsin : 0.3%
Medium
PBS
Eye
Human
Human, 5-65 years
Von Recum, H. , Okano, T. , Kim, S. and Bernstein, P.
Maintenance of Retinoid Metabolism in Human Retinal Pigment Epithelium Cell Culture ,
Exp Eye Res
 69 , 97 , 1999
Cells
Retinal pigment epithelial (RPE)
Enzymes
Trypsin : 0.25%
Medium
HBSS
Eye
Mouse
Mouse, 4-8 week
Kobayashi, T. , Yoshioka, R. , Shiraishi, A. and Ohashi, Y.
New Technique for Culturing Corneal Epithelial Cells of Normal Mice. ,
Mol Vis
 15 , 1589-93 , 2009
Cells
Corneal epithelial
Enzymes
Trypsin : 0.25%
Medium
DMEM/F12
Eye
Mouse
Mouse, adult
Yoshida, S. , Shimmura, S. , Nagoshi, N. , Fukuda, K. , Matsuzaki, Y. , Okano, H. and Tsubota, K.
Isolation of Multipotent Neural Crest-Derived Stem Cells from the Adult Mouse Cornea. ,
Stem Cells
 24 , 2714-22 , 2006
Cells
Stromal
Enzymes
Trypsin : 0.05%
Collagenase : 78 u/ml
Hyaluronidase : 38 u/ml
Medium
DMEM/F-12
Eye
Rabbit
Rabbit, New Zealand
Stramer Brian M, Kwok Michael G K, Farthing-Nayak Pamela J, Jung Jae-Chang, Fini M Elizabeth, Nayak Ramesh C.
Monoclonal antibody (3G5)-defined ganglioside: cell surface marker of corneal keratocytes ,
Inv Ophthal Visual Sci
 45 , 807-12 , 2004
Cells
Corneal keratocytes
Enzymes
Trypsin : 0.25%
Collagenase : 0.5%
Medium
PBS
Eye
Rabbit
Rabbit, New Zealand, adult, male, albino,
2 kg
Johnson-Muller, B. and Gross, J.
Regulation of Corneal Collagenase Production: Epithelial-Stromal Cell Interactions ,
Proc Natl Acad Sci U S A
 75 (9) , 4417 , 1978
Cells
Epithelial
Enzymes
Trypsin : 0.25%
Medium
HBSS/DMEM
Eye
Rat
Rat, embryonic 17 day
Seigel, G.
The Golden Age of Retinal Cell Culture ,
Mol Vis
 5 , 4 , 1999
Cells
Retinal
Enzymes
Trypsin : 0.6%
Medium
DMEM
Eye
Rat
Rat, SD, pups
Sarthy PV, Curtis BM. and Catterall WA.
Retrograde Labeling, Enrichment, and Characerization of Retinal Ganglion Cells from the Neonatal Rat ,
J Neurosci
 3 (12) , 2532 , 1983
Cells
Retina
Enzymes
Trypsin : 0.25%
Medium
Ham's F-12
Neural
Bovine
Bovine
(also rat)
Trifaro, J. , Tang, R. and Novas, M.
Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons ,
In Vitro Cell Dev Biol
 26 , 335 , 1990
Cells
Heart
Adrenal chromaffin
Paraneurons
Enzymes
Trypsin : 0.06%
Medium
25mM HEPES buffered Locke's solution, CMF
Neural
Bovine
Calf
(also lamb)
Poduslo, S. , Miller, K. and McKhann, G.
Metabolic Properties of Maintained Oligodendroglia Purified from Brain ,
J Biol Chem
 253 , 1592 , 1978
Cells
Oligodendroglia
Neural
Enzymes
Trypsin : 0.1%
Medium
(see reference)
Neural
Chicken
Chick, White Leghorn, embryos
Watt, P. , Mullen, Y. , Benhamou, P. , Hober, C. , Nomura, Y. , Watanabe, Y. , Passaro, E. , Zinner, M. and Brunicardi, F.
Simplified Semiautomated Method For Isolating Islets From the Human Pancreas ,
Transplant Proc
 26 (2) , 582 , 1994
Cells
Islets
Enzymes
Trypsin : Trypsin: 0.125-0.25%
Collagenase : 0.2%
Deoxyribonuclease I : 200 u/ml
Medium
Eurocollins solution
Neural
Chicken
Chick, embryo, 10-14 day
Moyer, M. , Bullrich, F. and Sheffield, J.
Emergence of Flat Cells From Glia in Stationary Cultures of Embryonic Chick Neural Retina ,
In Vitro Cell Dev Biol
 26 , 1073 , 1990
Cells
Flat, retina
Enzymes
Trypsin : 0.1%
Medium
Tyrode's solution, CMF
Neural
Chicken
Chick, White Leghorn, embryo
(also rat, SD, embryo)
Schnaar, R. and Schaffner, A.
Separation of cell types from embryonic chicken and rat spinal cord: characterization of motoneuron-enriched fractions ,
J Neurosci
 1 , 204 , 1981
Cells
Spinal cord
Enzymes
Trypsin : 0.05%
Medium
Phosphate buffer
(see reference)
Neural
Chicken
Chick
Choi, D. and Fiscbach, G.
GABA Conductance of Chick Spinal Cord and Dorsal Root Ganglion Neurons in Cell Culture ,
J Neurophysiol
 45 , 605 , 1981
Cells
Dorsal root ganglion neurons
Spinal cord
Enzymes
Trypsin : 0.1%
Medium
Puck's saline, CMF
Neural
Chicken
Chick, White Leghorn embryo, 8 day
Bottenstein, J. , Skaper, S. , Varon, S. and Sato, G.
Selective Survival of Neurons from Chick Embryo Sensory Ganglionic Dissociates Utilizing Serum-Free Supplemented Medium ,
Exp Cell Res
 125 , 183 , 1980
Cells
Neurons, ganglia
Enzymes
Trypsin : 0.25%
Medium
HBSS, CMF
Neural
Chicken
Chicken, White Leghorn, fertile eggs
Tuttle, J. , Suszkiw, J. and Ard, M.
Long-Term Survival and Development of Dissociated Parasympathetic Neurons in Culture ,
Brain Res
 183 , 161 , 1980
Cells
Ciliary ganglion neurons
Enzymes
Trypsin : 0.25%
Medium
Eagle's MEM
Neural
Chicken
Chick embryos
McCarthy, K. and Partlow, L.
Preparation of Pure Neuronal and Non-Neuronal Cultures From Embryonic Chick Sympathetic Ganglia: A New Method Based on Both Differential Cell Adhesiveness and the Formation of Homotypic Neuronal Aggregates ,
Brain Res
 114 , 391 , 1976
Cells
Ganglion chains, sympathetic ganglia
Enzymes
Trypsin : 0.25%
Medium
Krebs Phosphosaline
Neural
Chicken
Chick, White Leghorn, embryos, 7 day
Wiseman, L. and Hammond, W.
The Reacquisition of Cell Adhesiveness Following Tissue Disaggregation by Eleven Different Agents ,
J Exp Zool
 197 , 429 , 1976
Cells
Neural retina
Enzymes
Collagenase : 0.25%
Elastase : 0.2%
Hyaluronidase : 1.0%
Papain : 1.0%
Protease : 0.1%
Trypsin : 0.05%
Medium
HBSS
Neural
Fish
Zebrafish, Dania rerio, embryo
Won, Y. , Ono, F. and Ikeda, S.
Identification and Modulation of Voltage-Gated Ca2+ Currents in Zebrafish Rohon-Beard Neurons. ,
J Neurophysiol
 105 , 442-53 , 2011
Cells
Rohon-Beard neurons
Enzymes
Trypsin : 0.2%
Medium
L-15/Hepes
Neural
Fish
Black ghose knige fish, adult (Apteronotus albifrons)
Anderson, M.J.
Differences in Growth of Neurons from Normal and Regenerated Teleost Spinal Cord in vitro ,
In Vitro Cell Dev Biol
 29A , 145 , 1993
Cells
Neurons, spinal cord
Enzymes
Trypsin : 0.4%
Medium
PBS, CMF
Neural
Guinea-Pig
Guinea-pig, newborn
Jessen, K, McConnell, J. , Purves, R. , Burnstock, G. and Chamley-Campbell, J.
Tissue Culture of Mammalian Enteric Neurons ,
Brain Res
 152 , 573 , 1978
Cells
Neuron, enteric
Enzymes
Trypsin : 0.125%
Medium
Medium 199
Neural
Hamster
Hamster, male
Liman ER.
Regulation by voltage and adenine nucleotides of a Ca2+-activated cation channel from hamster vomeronasal sensory neurons ,
J Physiol
 548 , 777 , 2003
Cells
Vomeronasal organ neurons
Enzymes
Trypsin : 0.02%
Medium
PBS
Neural
Human
Human
Panchision David M, Chen Hui-Ling, Pistollato Francesca, Papini Daniela, Ni Hsiao-Tzu, Hawley Teresa S.
Optimized flow cytometric analysis of central nervous system tissue reveals novel functional relationships among cells expressing CD133, CD15, and CD24 ,
Stem Cells
 25 , 1560-70 , 2007
Cells
Neural, various
Enzymes
Papain : 12 u/ml
Collagenase/Dispase : see reference
Trypsin : see reference
Medium
see reference
Neural
Human
Human, 5-65 years
Von Recum, H. , Okano, T. , Kim, S. and Bernstein, P.
Maintenance of Retinoid Metabolism in Human Retinal Pigment Epithelium Cell Culture ,
Exp Eye Res
 69 , 97 , 1999
Cells
Retinal pigment epithelial (RPE)
Enzymes
Trypsin : 0.25%
Medium
HBSS
Neural
Mouse
Mouse
Xu, W. , Sachewsky, N. , Azimi, A. , Hung, M. , Gappasov, A. and Morshead, C.
Myelin Basic Protein Regulates Primitive and Definitive Neural Stem Cell Proliferation from the Adult Spinal Cord. ,
Stem Cells
 35 , 485-496 , 2017
Cells
Neurospheres
Enzymes
Trypsin : 0.13%
Hyaluronidase : 0.08%
Medium
Neurobasal A
Neural
Mouse
Mouse, day 2
Clements, M. , Byrne, E. , Camarillo Guerrero, L. , Cattin, A. , Zakka, L. , Ashraf, A. , Burden, J. , Khadayate, S. , Lloyd, A. , Marguerat, S. and Parrinello, S.
The Wound Microenvironment Reprograms Schwann Cells to Invasive Mesenchymal-like Cells to Drive Peripheral Nerve Regeneration. ,
Neuron
 96 , 98-114.e7 , 2017
Cells
Schwann
Enzymes
Medium
DMEM
Neural
Mouse
Mouse, neonatal
Stettner, M. , Lohmann, B. , Wolffram, K. , Weinberger, J. , Dehmel, T. , Hartung, H. , Mausberg, A. and Kieseier, B.
Interleukin-17 Impedes Schwann Cell-Mediated Myelination. ,
J Neuroinflammation
 11 , 63 , 2014
Cells
Schwann cells
Enzymes
Collagenase : 0.05-0.1%
Trypsin : 0.125-0.25%
Medium
DMEM
Neural
Mouse
Mouse
Lee, B. , Cho, H. , Jung, J. , Yang, Y. , Yang, D. and Oh, U.
Anoctamin 1 Contributes to Inflammatory and Nerve-Injury Induced Hypersensitivity. ,
Mol Pain
 10 , 5 , 2014
Cells
DRG neurons
Enzymes
Trypsin : 0.25%
Medium
DMEM/F-12
Neural
Mouse
Mouse
Zhang, Y. and Hu, W.
Mouse Enteric Neuronal Cell Culture. ,
Methods Mol Biol
 1078 , 55-63 , 2013
Cells
Enteric neurons
Enzymes
Trypsin : 0.05%
Deoxyribonuclease I : 400 u/ml
Medium
DMEM/F12
Neural
Mouse
Mouse, 4-6 week
Masaki, T. , Qu, J. , Cholewa-Waclaw, J. , Burr, K. , Raaum, R. and Rambukkana, A.
Reprogramming Adult Schwann Cells to Stem Cell-Like Cells by Leprosy Bacilli Promotes Dissemination of Infection. ,
Cell
 152 , 51 , 2013
Cells
Schwann
Enzymes
Trypsin : 0.125%
Medium
DMEM
Neural
Mouse
Mouse, 6-8 week
Babona-Pilipos, R. , Popovic, M. and Morshead, C.
A Galvanotaxis Assay for Analysis of Neural Precursor Cell Migration Kinetics in an Externally Applied Direct Current Electric Field. ,
J Vis Exp
 68 , e4193 , 2012
Cells
Neural precursors
Enzymes
Trypsin : 0.13%
Hyaluronidase : 0.08%
Medium
see reference
Neural
Mouse
Mouse, P4-5
Ziegler, A. , Schneider, J. , Qin, M. , Tyler, W. , Pintar, J. , Fraidenraich, D. , Wood, T. and Levison, S.
IGF-II Promotes Stemness of Neural Restricted Precursors. ,
Stem Cells
 30 , 1265 , 2012
Cells
Neuroshere
Enzymes
Trypsin : 0.25%
Papain : 100 u
Medium
Pro-N

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.