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Tissue References Search Tool

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Eye
Chicken
Chick, embryo, 6 day
Jacob Vanessa, Rothermel Andrae, Wolf Peter, Layer Paul G.
Rhodopsin, violet and blue opsin expressions in the chick are highly dependent on tissue and serum conditions ,
Cell Death Differ
 180 , 159-68 , 2005
Cells
Retinal cells
Enzymes
Trypsin : 0.005%
Medium
DMEM/F12
Eye
Chicken
Chick embryo
Reenstra Wende R, Orlow Daniel L, Svoboda Kathy K H.
ECM-stimulated signaling and actin reorganization in embryonic corneal epithelia are Rho dependent ,
Inv Ophthal Visual Sci
 43 , 3181-9 , 2002
Cells
Corneal epithelia
Enzymes
Collagenase : 0.08%
Trypsin : 0.08%
Medium
HBSS
Eye
Chicken
Chick embryo
(also rat)
Seigel, G.
The Golden Age of Retinal Cell Culture ,
Mol Vis
 5 , 4 , 1999
Cells
Retinal
Enzymes
Trypsin : 0.6%
Medium
DMEM
Eye
Chicken
Chick, embryo, 10-14 day
Moyer, M. , Bullrich, F. and Sheffield, J.
Emergence of Flat Cells From Glia in Stationary Cultures of Embryonic Chick Neural Retina ,
In Vitro Cell Dev Biol
 26 , 1073 , 1990
Cells
Flat, retina
Enzymes
Trypsin : 0.1%
Medium
Tyrode's solution, CMF
Eye
Fish

Zebrafish, Danio rerio

Middel, C. , Dietrich, N. , Hammes, H. and Kroll, J.
Analysis of the Morphology of Retinal Vascular Cells in Zebrafish , Front Cell Dev Biol Vol. 11 , 1267232 , 2023
Cells
Retinal vascular
Enzymes
Trypsin : 0.3%
Medium
PBS
Eye
Human
Human, 5-65 years
Von Recum, H. , Okano, T. , Kim, S. and Bernstein, P.
Maintenance of Retinoid Metabolism in Human Retinal Pigment Epithelium Cell Culture ,
Exp Eye Res
 69 , 97 , 1999
Cells
Retinal pigment epithelial (RPE)
Enzymes
Trypsin : 0.25%
Medium
HBSS
Eye
Mouse
Mouse, 4-8 week
Kobayashi, T. , Yoshioka, R. , Shiraishi, A. and Ohashi, Y.
New Technique for Culturing Corneal Epithelial Cells of Normal Mice. ,
Mol Vis
 15 , 1589-93 , 2009
Cells
Corneal epithelial
Enzymes
Trypsin : 0.25%
Medium
DMEM/F12
Eye
Mouse
Mouse, adult
Yoshida, S. , Shimmura, S. , Nagoshi, N. , Fukuda, K. , Matsuzaki, Y. , Okano, H. and Tsubota, K.
Isolation of Multipotent Neural Crest-Derived Stem Cells from the Adult Mouse Cornea. ,
Stem Cells
 24 , 2714-22 , 2006
Cells
Stromal
Enzymes
Trypsin : 0.05%
Collagenase : 78 u/ml
Hyaluronidase : 38 u/ml
Medium
DMEM/F-12
Eye
Rabbit
Rabbit, New Zealand
Stramer Brian M, Kwok Michael G K, Farthing-Nayak Pamela J, Jung Jae-Chang, Fini M Elizabeth, Nayak Ramesh C.
Monoclonal antibody (3G5)-defined ganglioside: cell surface marker of corneal keratocytes ,
Inv Ophthal Visual Sci
 45 , 807-12 , 2004
Cells
Corneal keratocytes
Enzymes
Trypsin : 0.25%
Collagenase : 0.5%
Medium
PBS
Eye
Rabbit
Rabbit, New Zealand, adult, male, albino,
2 kg
Johnson-Muller, B. and Gross, J.
Regulation of Corneal Collagenase Production: Epithelial-Stromal Cell Interactions ,
Proc Natl Acad Sci U S A
 75 (9) , 4417 , 1978
Cells
Epithelial
Enzymes
Trypsin : 0.25%
Medium
HBSS/DMEM
Eye
Rat
Rat, embryonic 17 day
Seigel, G.
The Golden Age of Retinal Cell Culture ,
Mol Vis
 5 , 4 , 1999
Cells
Retinal
Enzymes
Trypsin : 0.6%
Medium
DMEM
Eye
Rat
Rat, SD, pups
Sarthy PV, Curtis BM. and Catterall WA.
Retrograde Labeling, Enrichment, and Characerization of Retinal Ganglion Cells from the Neonatal Rat ,
J Neurosci
 3 (12) , 2532 , 1983
Cells
Retina
Enzymes
Trypsin : 0.25%
Medium
Ham's F-12
Stem
Canine
Canine
Park, S. , Seo, M. , Kim, H. and Kang, K.
Isolation and Characterization of Canine Amniotic Membrane-Derived Multipotent Stem Cells. ,
PLoS ONE
 7 , e44693 , 2012
Cells
Amniotic membrane MSC
Enzymes
Trypsin : 0.25%
Medium
LG-DMEM
Stem
Human
Human
Kusuma, G. , Menicanin, D. , Gronthos, S. , Manuelpillai, U. , Abumaree, M. , Pertile, M. , Brennecke, S. and Kalionis, B.
Ectopic Bone Formation by Mesenchymal Stem Cells Derived from Human Term Placenta and the Decidua. ,
PLoS ONE
 10 , e0141246 , 2015
Cells
Decidua mesenchymal stem
Enzymes
Medium
PBS
Stem
Human
Human
Hang, H. , Yu, Y. , Wu, N. , Huang, Q. , Xia, Q. and Bian, J.
Induction of Highly Functional Hepatocytes from Human Umbilical Cord Mesenchymal Stem Cells by HNF4α Transduction. ,
PLoS ONE
 9 , e104133 , 2014
Cells
Umbilical mesenchymal stem
Enzymes
Trypsin : 0.25%
Medium
DMEM
Stem
Human
Human
Barkauskas, C. , Cronce, M. , Rackley, C. , Bowie, E. , Keene, D. , Stripp, B. , Randell, S. , Noble, P. and Hogan, B.
Type 2 Alveolar Cells are Stem Cells in Adult Lung ,
J Clin Invest
 123 , 3025 , 2013
Cells
Alveolar epithelial
Enzymes
Neutral Protease : 2 u/ml
Trypsin : 0.25%
Elastase : 10 u/ml
Medium
Bronchial Epithelial Growth Medium
Stem
Human
Human
Dobbin, Z. and Landen, C.
Isolation and Characterization of Potential Cancer Stem Cells from Solid Human Tumors--Potential Applications. ,
Curr Protoc Pharmacol
 63 , Unit 14.28. , 2013
Cells
Cancer stem
Enzymes
Trypsin : 0.25%
Collagenase : 0.05%
Hyaluronidase : 0.005%
Medium
RPMI
Stem
Human
Human
Han, Y. , Tao, R. , Sun, T. , Chai, J. , Xu, G. and Liu, J.
Optimization of Human Umbilical Cord Mesenchymal Stem Cell Isolation and Culture Methods. ,
Cytotechnology
 65 , 819 , 2013
Cells
Umbilical cord mesenchymal stem
Enzymes
Collagenase : 0.1-0.2%
Trypsin : 0.1%
Medium
DMEM
Stem
Human
Human, adult
Nagel, S. , Rohr, F. , Weber, C. , Kier, J. , Siemers, F. , Kruse, C. , Danner, S. , Brandenburger, M. and Matthiessen, A.
Multipotent Nestin-Positive Stem Cells Reside in the Stroma of Human Eccrine and Apocrine Sweat Glands and can be Propagated Robustly In Vitro. ,
PLoS ONE
 8 , e78365 , 2013
Cells
Epidermal stem cells
Enzymes
Neutral Protease : 2 u/ml
Trypsin : 2.5%
Medium
DMEM
Stem
Human
Human
Salehinejad, P. , Alitheen, N. , Ali, A. , Omar, A. , Mohit, M. , Janzamin, E. , Samani, F. , Torshizi, Z. and Nematollahi-Mahani, S.
Comparison of Different Methods for the Isolation of Mesenchymal Stem Cells from Human Umbilical Cord Wharton's Jelly. ,
In Vitro Cell Dev Biol Anim
 48 , 75 , 2012
Cells
Wharton jelly mesenchymal stem
Enzymes
Trypsin : 0.25%
Medium
DMEM
Stem
Human
Human
Nazarov, I. , Lee, J. , Soupene, E. , Etemad, S. , Knapik, D. , Green, W. , Bashkirova, E. , Fang, X. , Matthay, M. , Kuypers, F. and Serikov, V.
Multipotent Stromal Stem Cells from Human Placenta Demonstrate High Therapeutic Potential. ,
Stem Cells Transl Med
 1 , 359-72 , 2012
Cells
Chorionic mesenchymal
Enzymes
Neutral Protease : 2.5 u/ml
Trypsin : 0.25%
Medium
MEM
Stem
Human
Human
Varghese, M. , Olstorn, H. , Berg-Johnsen, J. , Moe, M. , Murrell, W. and Langmoen, I.
Isolation of Human Multipotent Neural Progenitors from Adult Filum Terminale. ,
Stem Cells Dev
 18 , 603 , 2009
Cells
Filum terminale neural progenitor
Enzymes
Trypsin : see reference
Medium
DMEM/F-12
Stem
Human
Human, male
Kossack, N. , Meneses, J. , Shefi, S. , Nguyen, H. , Chavez, S. , Nicholas, C. , Gromoll, J. , Turek, P. and Reijo-Pera, R.
Isolation and Characterization of Pluripotent Human Spermatogonial Stem Cell-Derived Cells. ,
Stem Cells
 , 2008
Cells
Spermatogonial stem cells
Enzymes
Medium
DMEM
Stem
Human
Human
Nakamura, T. , Endo, K. and Kinoshita, S.
Identification of Human Oral Keratinocyte Stem/Progenitor Cells by Neurotrophin Receptor p75 and the Role of Neurotrophin/p75 Signaling. ,
Stem Cells
 25 , 628-38 , 2007
Cells
Oral epithelial
Enzymes
Neutral Protease : 1.2 u/ml
Trypsin : 0.05%
Medium
PBS
Stem
Human
Human
Welter, J. and Solchaga, L.and Penick, K.
Simplification of Aggregate Culture of Human Mesenchymal Stem Cells as a Chondrogenic Screening Assay ,
Biotechniques
 42 , 732 , 2007
Cells
Bone marrow derived MSC
Enzymes
Trypsin : 0.05%
Papain : 0.0025%
Medium
DMEM
Stem
Human
Human
Yu Hong, Fang Dong, Kumar Suresh M, Li Ling, Nguyen Thiennga K, Acs Geza, Herlyn Meenhard, Xu Xiaowei.
Isolation of a novel population of multipotent adult stem cells from human hair follicles ,
Am J Pathol
 168 , 1879-88 , 2006
Cells
Hair follicular epithelial
Enzymes
Trypsin : 0.25%
Medium
DMEM/F-12
Stem
Human
Human
Alessandri Giulio, Pagano Stefano, Bez Alessandra, Benetti Anna, Pozzi Stefano, Iannolo Gioacchin, Baronio Manuela, Invernici Gloria, Caruso Arnaldo, Muneretto Claudio, Bisleri Gianluigi, Parati Eugenio.
Isolation and culture of human muscle-derived stem cells able to differentiate into myogenic and neurogenic cell lineages ,
Lancet
 364 , 1872-83
Cells
Muscle-derived stem cells
Enzymes
Trypsin : 0.25%
Medium
DMEM/F12
Stem
Monkey
Baboons, 1yr, 7yr, 14 yr
Nagano, M. , McCarrey, J. and Brinster, R.
Primate Spermatogonial Stem Cells Colonize Mouse Testes ,
Biol Reprod
 64 , 1409 , 2001
Cells
Primate spermatogonial
Enzymes
Medium
DMEM
Stem
Mouse
Mouse, adult
Martin, L. and Seandel, M.
Serial Enrichment of Spermatogonial Stem and Progenitor Cells (SSCs) in Culture for Derivation of Long-Term Adult Mouse SSC Lines. ,
J Vis Exp
 72 , e50017 , 2013
Cells
Spermatogonial stem
Enzymes
Trypsin : 0.05%
Medium
DMEM
Stem
Mouse
Mouse
Jensen, U. , Ghazizadeh, S. and Owens, D.
Isolation and Characterization of Cutaneous Epithelial Stem Cells. ,
Methods Mol Biol
 989 , 61-9 , 2013
Cells
Epithelial stem
Enzymes
Trypsin : 0.25%
Medium
HBSS
Stem
Mouse
Mouse, 4-6 week
Masaki, T. , Qu, J. , Cholewa-Waclaw, J. , Burr, K. , Raaum, R. and Rambukkana, A.
Reprogramming Adult Schwann Cells to Stem Cell-Like Cells by Leprosy Bacilli Promotes Dissemination of Infection. ,
Cell
 152 , 51 , 2013
Cells
Schwann
Enzymes
Trypsin : 0.125%
Medium
DMEM
Stem
Mouse
Mouse, P4-5
Ziegler, A. , Schneider, J. , Qin, M. , Tyler, W. , Pintar, J. , Fraidenraich, D. , Wood, T. and Levison, S.
IGF-II Promotes Stemness of Neural Restricted Precursors. ,
Stem Cells
 30 , 1265 , 2012
Cells
Neuroshere
Enzymes
Trypsin : 0.25%
Papain : 100 u
Medium
Pro-N
Stem
Mouse
Mouse, adult
Deleyrolle, L. and Reynolds, B.
Isolation, Expansion, and Differentiation of Adult Mammalian Neural Stem and Progenitor Cells using the Neurosphere Assay. ,
Methods Mol Biol
 549 , 91 , 2009
Cells
Adult neural stem
Enzymes
Trypsin : 0.05%
Medium
DMEM
Stem
Mouse
Mouse, 6-8 week
Burger, P. , Gupta, R. , Xiong, X. , Ontiveros, C. , Salm, S. , Moscatelli, D. and Wilson, E.
High Aldehyde Dehydrogenase Activity: A Novel Functional Marker of Murine Prostate Stem/Progenitor Cells. ,
Stem Cells
 27 , 2220-8 , 2009
Cells
Prostate epithelial/stem
Enzymes
Trypsin : 0.05%
Medium
HBSS
Stem
Mouse
Mouse, postnatal
Sher, F. , Rossler, R. , Brouwer, N. , Balasubramaniyan, V. , Boddeke, E. and Copray, S.
Differentiation of Neural Stem Cells Into Oligodendrocytes: Involvement of the Polycomb Group Protein Ezh2. ,
Stem Cells
 26 , 2875 , 2008
Cells
Astrocytes
Enzymes
Trypsin : 0.25%
Deoxyribonuclease I : 1,000 u/ml
Medium
HBSS
Stem
Mouse
Mouse, 12 week
Taddei, I. , Deugnier, M. , Faraldo, M. , Petit, V. , Bouvard, D. , Medina, D. , Fassler, R. , Thiery, J. and Glukhova, M.
Beta1 Integrin Deletion from the Basal Compartment of the Mammary Epithelium Affects Stem Cells ,
Nat Cell Biol
 10 , 716-22 , 2008
Cells
Mammary epithelial
Enzymes
Collagenase : 0.3%
Hyaluronidase : 100 u/ml
Trypsin : 0.25%
Medium
see reference

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.