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Tissue References Search Tool

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Mammary
Mouse
Mouse, 10 week
Cheng, N. and Lambert, D.
Mammary Transplantation of Stromal Cells and Carcinoma Cells in C57BL/6J Mice. ,
J Vis Exp
 54 , e2716 , 2011
Cells
Fibroblasts, carcinoma
Enzymes
Trypsin : 0.2%
Hyaluronidase : 1,000 u/ml
Medium
DMEM
Mammary
Mouse
Mouse, 12 week
Taddei, I. , Deugnier, M. , Faraldo, M. , Petit, V. , Bouvard, D. , Medina, D. , Fassler, R. , Thiery, J. and Glukhova, M.
Beta1 Integrin Deletion from the Basal Compartment of the Mammary Epithelium Affects Stem Cells ,
Nat Cell Biol
 10 , 716-22 , 2008
Cells
Mammary epithelial
Enzymes
Collagenase : 0.3%
Hyaluronidase : 100 u/ml
Trypsin : 0.25%
Medium
see reference
Mammary
Mouse
Mouse, BALB/c, virgin, female, 4 month
Beck, J. , Hosick, H. and Watkins, B.
Growth of Epithelium From a Preneoplastic Mammary Outgrowth on Response to Mammary Adipose Tissue ,
In Vitro Cell Dev Biol
 25 (5) , 409 , 1989
Cells
Adipocytes
Enzymes
Trypsin : 50 µg/ml
Medium
DMEM
Miscellaneous
Human
Human
Nakamura, T. , Endo, K. and Kinoshita, S.
Identification of Human Oral Keratinocyte Stem/Progenitor Cells by Neurotrophin Receptor p75 and the Role of Neurotrophin/p75 Signaling. ,
Stem Cells
 25 , 628-38 , 2007
Cells
Oral epithelial
Enzymes
Neutral Protease : 1.2 u/ml
Trypsin : 0.05%
Medium
PBS
Miscellaneous
Invertebrate

Octopus vulgaris

Maselli, V. , Xu, F. , Syed, N. , Polese, G. and Cosmo, A.
A Novel Approach to Primary Cell Culture for Octopus vulgaris Neurons. ,
Front Physiol
 9 , 220 , 2018
Cells
Neurons
Enzymes
Papain : 0.1%
Trypsin : 0.1%
Medium
L15-ASW
Miscellaneous
Mouse
Mouse, embryonic
He, M. , Wu, B. , Ye, W. , Le, D. , Sinclair, A. , Padovano, V. , Chen, Y. , Li, K. , Sit, R. , Tan, M. , Caplan, M. , Neff, N. , Jan, Y. , Darmanis, S. and Jan, L.
Chloride Channels Regulate Differentiation and Barrier Functions of the Mammalian Airway. ,
Elife
 9 , 53085 , 2020
Cells
Trachea
Enzymes
Elastase : 0.1%
Trypsin : 0.125%
Medium
DMEM/F12
Miscellaneous
Mouse
Mouse
Kim, Y. , Wang, S. , Tymanskyj, S. , Ma, L. , Tao, H. and Zhang, L.
Dcc Mediates Functional Assembly of Peripheral Auditory Circuits. ,
Sci Rep
 6 , 23799 , 2016
Cells
Cochlear
Enzymes
Papain : 20 u/ml
Trypsin : 0.05%
Medium
DMEM
Miscellaneous
Mouse
Mouse
Jan, T. , Chai, R. , Sayyid, Z. and Cheng, A.
Isolating LacZ-Expressing Cells from Mouse Inner Ear Tissues Using Flow Cytometry. ,
J Vis Exp
 58 , e3432 , 2011
Cells
Cochleaer
Enzymes
Trypsin : 0.125%
Medium
DMEM/F-12
Miscellaneous
Porcine
Porcine, 6 month
Kean, T. and Dennis, J.
Synoviocyte Derived-Extracellular Matrix Enhances Human Articular Chondrocyte Proliferation and Maintains Re-Differentiation Capacity at Both Low and Atmospheric Oxygen Tensions. ,
PLoS ONE
 10 , e0129961 , 2015
Cells
Synoviocytes
Enzymes
Hyaluronidase : 660 u/ml
Trypsin : 0.25%
Collagenase Type 2 : 583 u/ml
Medium
DMEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.