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Tissue References Search Tool

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Brain
Rat
Rat, SD, Fisher
Brewer, G.J.
Isolation and culture of adult rat hippocampal neurons of any age ,
J Neurosci Methods
 71 , 143 , 1997
Cells
Hippocampal neurons
Enzymes
Papain : 0.2%
Medium
HibernateA/B27
Brain
Rat
Rat, E18
Naeve GS, Ramakrishnan M, Kramer R, Hevroni D, Citri Y, Theill LE.
Neuritin: a gene induced by neural activity and neurotrophins that promotes neuritogenesis ,
Proc Natl Acad Sci U S A
 94 , 2648-53 , 1997
Cells
Hippocampal and cortical neurons
Enzymes
PDS kit : per instructions
Medium
Neurobasal media
Brain
Rat
Rat, newborn
Holzwarth, J. , Glaum, S. and Miller, R.
Activation of Endothelin Receptors by Sarafotoxin Regulates Ca2+ Homeostasis in Cerebellar Astrocytes ,
Glia
 5 , 239 , 1992
Cells
Astrocytes
Enzymes
Trypsin : 0.25%
Medium
DMEM, HBSS
Brain
Rat
Rat, SD, newborn
Pixley, S.K.
The Olfactory Nerve Contains Two Populations of Glia ,
In Vitro
 5 , 269 , 1992
Cells
Glial
Enzymes
Trypsin : 0.0625%
Medium
MEM, sterile
Brain
Rat
Rat, 60-72 hours old
Acheson, A. , Barker, P. , Alderson, R. , Miller, F. and Murphy, R.
Detection of Brain-Derived Neurotrophic Factor-like Activity in Fibroblasts and Schwann Cells: Inhibition by Antibodies to NGF ,
Neuron
 7 , 265 , 1991
Cells
Fibroblasts
Enzymes
Trypsin : 0.2%
Medium
HEPES buffered DMEM
Brain
Rat
Rat, SD, adult
Doron, D. , Jacobowitz, D. , Heldman, E. , Feurerstein, G. , Pollard, H. and Hallenbeck, J.
Extracellular Matrix Permits the Expression of Von Willebrand's Factor, Uptake of Di-I-Acetylated Low Density Lipoprotein and Secretion of Prostacyclin in Cultures of Endothelial Cells from Rat Brain Microvessels ,
In Vitro Cell Dev Biol
 27A , 689 , 1991
Cells
Endothelial
Enzymes
Medium
Medium 199
Brain
Rat
Rat, SD, 5 day
Mattson, M. and Kater, S.
Development and Selective Neurodegeneration in Cell Cultures From Different Hippocampal Regions ,
Brain Res
 490 , 110 , 1989
Cells
Hippocampal neurons
Enzymes
Trypsin : 0.2%
Deoxyribonuclease I : 10 ug/ml
Medium
HBSS
Brain
Rat
Rat, SD, female
Matsuda, M.
Serum Proteins Enhance Aggregate Formation of Dissociated Fetal Rat Brain Cells in an Aggregating Culture ,
In Vitro Cell Dev Biol
 24 (10) , 1031 , 1988
Cells
Fetal rat brain
Enzymes
Medium
DMEM
Brain
Rat
Rat, albino, adult and newborn
Giulian, D. and Baker, T.J.
Characterization of Ameboid Microglia Isolated From Developing Mammalian Brain ,
J Neurosci
 6 , 2163 , 1986
Cells
Cerebral cortices
Enzymes
Trypsin : 0.25%
Medium
PBS
Brain
Rat
Rat, Wistar, newborn
Goldman, J.E. , Geier, S.S. and Hirano, M.
Differentiation of Astrocytes and Oligodendrocytes From Germinal Matrix Cells in Primary Culture ,
J Neurosci
 6 , 52 , 1986
Cells
Germinal matrix
Enzymes
Trypsin : 0.25%
Medium
HBSS
Brain
Rat
Rat, fetus, 18 day
Rothman, S.
Synaptic Release of Excitatory Amino Acid Neurotransmitter Mediates Anoxic Neuronal Death ,
J Neurosci
 4 (7) , 1884 , 1984
Cells
Hippocampi
Enzymes
Trypsin : 0.1%
Medium
HBSS
Brain
Rat
Rat, fetus, 18-20 day
Bartlett, W. and Banker, G.
An Electron Microscopic Study of the Development of Axons and Dendrites by Hippocampal Neurons in Culture. I. Cells Which Develop Without Intercellular Contacts ,
J Neurosci
 4 , 1944 , 1984
Cells
Hippocampal neurons
Enzymes
Trypsin : 0.25%
Medium
HBSS, CMF
Brain
Rat
Rat, SD, 19-20 days pregnant
Ahmed, Z. , Walker, P. and Fellows, R.
Properties of Neurons from Dissociated Fetal Rat Brain in Serum-Free Culture ,
J Neurosci
 3 (12) , 2448 , 1983
Cells
Neural
Enzymes
Trypsin : 0.25%
Medium
DMEM
Brain
Rat
Rat, SD, fetal
Peterfreund, R. and Vale, W.
High Molecular Weight Somatostatin Secretion by Cultured Rat Brain Cell ,
Brain Res
 239 , 463 , 1982
Cells
Cerebral cortex
Hypothalmus
Enzymes
Medium
HEPES
Brain
Rat
Rat, fetus
Swaiman, K. , Neale, E. , Fitzgerald, S. and Nelson, P.
A Method for Large-scale Production of Mouse Brain Cortical Cultures ,
Brain Res
 255 , 361 , 1982
Cells
Cortical
Enzymes
Trypsin : 0.25%
Medium
Puck's D1 (see reference)
Brain
Rat
Rat, Wistar-Kyoto, male, 100 - 200 g
Diglio, C.A. , Grammas, P. , Filiberto Giacomelli, M.S. and Wiener, J.
Primary Culture of Rat Cerebral Microvascular Endothelial Cells ,
Lab Invest
 46 , 554 , 1982
Cells
Endothelial
Cerebral
Enzymes
Medium
HBSS
Brain
Rat
Rat, Wistar, pregnant
Abney, E. , Bartlett, P. and Raff, M.
Astrocytes, Ependymal Cells, and Oligodendrocytes Develop on Schedule in Dissociated Cell Cultures of Embryonic Rat Brain ,
Dev Biol
 83 , 301 , 1981
Cells
Glial
Enzymes
Trypsin : 0.05%
Medium
Eagle's MEM/DMEM
Brain
Rat
Rat, SD, one month old
Bowman, P. , Betz, A. , Ar, D. , Wolinsky, J. , Penney, J. , Shivers, R. and Goldstein, G.
Primary Culture of Capillary Endothelium From Rat Brain ,
In Vitro
 17 (4) , 353 , 1981
Cells
Capillary endothelium
Pericytes
Enzymes
Medium
Medium 199
Brain
Rat
Rat, Wistar, male, 300-500 g
Williams, S. , Gillis, J. , Matthews, M. , Wagner, R. and Bitensky, M.
Isolation and Characterization of Brain Endothelial Cells: Morphology and Enzyme Activity ,
J Neurochem
 35 (2) , 374 , 1980
Cells
Microvessels
Endothelial
Enzymes
Medium
Ringers-HEPES buffer
Brain
Rat
Rat, Wistar, adult, 170 g
Phillips, P. , Kumar, P. , Kumar, S. and Waghe, M.
Isolation And Characterization of Endothelial Cells From Rat And Cow Brain White Matter ,
J Anat
 129 , 261 , 1979
Cells
Endothelial
Enzymes
Trypsin : 0.5%
Medium
BSS
Brain
Rat
Rat, Holtzmann, 18 day
Banker, G. and Cowan, M.
Rat Hippocampal Neurons in Dispersed Cell Culture ,
Brain Res
 126 , 397 , 1977
Cells
Hippocampal neurons
Enzymes
Trypsin : 0.1%
Medium
HBSS
Brain
Rat
Rat, SD, 2 days
Rowe, V. , Neale, E. , Avins, L. , Guroff, G. and Schrier, B.
Pineal gland cells in culture. Morphology, Biochemistry, Differentiation, and co-culture with sympathetic neurons ,
Exp Cell Res
 104 , 345 , 1977
Cells
Pineal
Enzymes
Trypsin : 0.25%
Medium
DMEM, MEM
Cartilage
Bovine
Bovine, 18-36 month
White, R. and Gibson, J.
The Effect of Oxygen Tension on Calcium Homeostasis in Bovine Articular Chondrocytes. ,
J Orthop Surg Res
 5 , 27 , 2010
Cells
Chondrocytes
Enzymes
Medium
DMEM
Cartilage
Bovine
Bovine, 6-8 week
Hwang, Y. , Sangaj, N. and Varghese, S.
Interconnected Macroporous Poly(ethylene glycol) Cryogels as a Cell Scaffold for Cartilage Tissue Engineering. ,
Tissue Eng Part A
 16 , 3033-41 , 2010
Cells
Chondrocytes
Enzymes
Medium
DMEM
Cartilage
Bovine
Bovine, 1-3 month
Lima, E. , Tan, A. , Tai, T. , Marra, K. , DeFail, A. , Ateshian, G. and Hung, C.
Genipin Enhances the Mechanical Properties of Tissue-Engineered Cartilage and Protects Against Inflammatory Degradation When Used as a Medium Supplement. ,
J Biomed Mater Res
 91 , 692 , 2009
Cells
Chondrocytes
Enzymes
Collagenase Type 4 : 390 u/ml
Medium
DMEM
Cartilage
Bovine
Steers, 1-2 years
Mackintosh, D. and Mason, R.
Pharmacological Actions of 17 Beta-oestradiol on Articular Cartilage Chondrocytes and Chondrosarcoma Chondrocytes in the Absence of Oestrogen Receptors ,
Biochim Biophys Acta
 964 , 295 , 1988
Cells
Chondrocytes
Enzymes
Trypsin : 0.20%
Medium
HBSS
Cartilage
Bovine
Calf, 1-14 days
Klagsbrun, M.
Large Scale Preparation of Chondrocytes , Methods of Enzymology Vol. 58 , 560 , 1979
Cells
Chondrocytes
Enzymes
Medium
PBS
Cartilage
Rat
Rat
Mei, L. , Shen, B. , Ling, P. , Liu, S. , Xue, J. , Liu, F. , Shao, H. , Chen, J. , Ma, A. and Liu, X.
Culture-expanded Allogenic Adipose Tissue-derived Stem Cells Attenuate Cartilage Degeneration in an Experimental Rat Osteoarthritis Model. ,
PLoS ONE
 12 , e0176107 , 2017
Cells
Chondrocytes
Enzymes
Trypsin : 0.25%
Medium
DMEM/F12
Cartilage
Rat
Rat, SD, young, 100-120 g
Shimomura, Y. , Yoneda, T. and Suzuki, F.
Osteogenesis by Chondrocytes from Growth Cartilage of Rat Rib ,
Calcif Tissue Res
 19 , 179 , 1975
Cells
Chondrocytes
Enzymes
Trypsin : 0.2%
Medium
Ham's F-12 medium
Colon
Rat
Rat, adult
Somara, S. , Bashllari, D. , Gilmont, R. and Bitar, K.
Real-Time Dynamic Movement of Caveolin-1 During Smooth Muscle Contraction of Human Colon and Aged Rat Colon Transfected with Caveolin-1 cDNA. ,
Am J Physiol Gastrointest Liver Physiol
 300 , G1022 , 2011
Cells
Colon smooth muscle
Enzymes
Medium
DMEM
Endothelial
Bovine
Bovine
Ricken Albert M, Traenkner Anja, Merkwitz Claudia, Hummitzsch Katja, Grosche Jens, Spanel-Borowski Katharina.
The short prolactin receptor predominates in endothelial cells of micro- and macrovascular origin ,
J Vasc Res
 44 , 19-30 , 2007
Cells
Bovine umbilical cord (BUVEC)
Enzymes
Collagenase : 0.1%
Medium
Dulbecco's/Ham F-12
Endothelial
Bovine
Bovine
Yu, M. , McAndrew, R. , Al-Saghir, R. , Maier, K. , Medhora, M. , Roman, R. and Jacobs, E.
Nitric Oxide Contributes to 20-HETE-Induced Relaxation of Pulmonary Arteries. ,
J Appl Physiol
 93 , 1391 , 2002
Cells
Pulmonary artery endothelial and smooth muscle cells
Enzymes
Collagenase : 0.04-0.05%
Medium
RPMI-1640
Endothelial
Bovine
Calf
Vender, R.
Role of Endothelial Cells in the Proliferative Response of Cultured Pulmonary Vascular Smooth Muscle Cells to Reduced Oxygen Tension ,
In Vitro Cell Dev Biol
 28A , 403 , 1992
Cells
Endothelial
Enzymes
Trypsin : 0.25%
Medium
HEPES
Endothelial
Bovine
Bovine, (Bos taurus), calf
Del Vecchio, P. , Siflinger-Birnboim, A. , Belloni, P. , Holleran, L. , Lum, H. and Malik, A.
Culture and Characterization of Pulmonary Microvascular Endothelial Cells ,
In Vitro Cell Dev Biol
 28A , 711 , 1992
Cells
Endothelial
Pulmonary
Enzymes
Collagenase : 1000 u/ml
Collagenase Type 2 : 1000 u/ml
Medium
PBS, CMF
Endothelial
Bovine
Bovine, adult
Machi, T. , Kassell, N.F. and Scheld, W.M.
Isolation and Characterization of Endothelial Cells From Bovine Cerebral Arteries ,
In Vitro Cell Dev Biol
 26 , 291 , 1990
Cells
Cerebral artery
Endothelial
Enzymes
Collagenase : 0.2%
Medium
HBSS
Endothelial
Bovine
Bovine
Martin, T.
Formation of Diacylglycerol by a Phospholipase D-phosphatidate Phosphatase Pathway Specific for Phosphatidylcholine in Endothelial Cells ,
Biochim Biophys Acta
 962 , 282 , 1988
Cells
Endothelial, pulmonary artery
Enzymes
Collagenase : 0.1%
Medium
CMF-Dulbecco's PBS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.