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Tissue References Search Tool

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Eye
Mouse
Mouse, 4 week
Su X, Sorenson CM. and Sheibani N.
Isolation and characterization of murine retinal endothelial cells ,
Mol Vis
 9 , 171 , 2003
Cells
Retinal endothelial cells
Enzymes
Medium
DMEM
Heart
Canine
Canine
Zhang,D. , Wu, C. , Qi, X. , Meijering, R. , Hoogstra-Berends, F. , Tadevosyan, A. and Cubukcuoglu, et al.
Activation of Histone Deacetylase-6 Induces Contractile Dysfunction Through Derailment of alpha-tubulin Proteostasis in Experimental and Human Atrial Fibrillation. ,
Circulation
 129 , 346-58 , 2014
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 150 u/ml
Medium
Tyrode's solution
Heart
Canine
Canine
Zhang, H. , Silva, J. , Lin, Y. , Verbsky, J. , Lee, U. , Kanter, E. , Yamada, K. , Schuessler, R. and Nichols, C.
Heterogeneity and Function of K(ATP) Channels in Canine Hearts. ,
Heart Rhythm
 10 , 1576-83 , 2013
Cells
Atrial and ventricular myocytes
Enzymes
Collagenase Type 2 : 0.05-0.08%
Medium
See reference
Heart
Canine
Canine, adult
Bonilla, I. , Sridhar, A. , Nishijima, Y. , Gyorke, S. , Cardounel, A. and Carnes, C.
Differential Effects of the Peroxynitrite Donor, SIN-1, on Atrial and Ventricular Myocyte Electrophysiology. ,
J Cardiovasc Pharmacol
 61 , 401-7 , 2013
Cells
Atrial and ventricular myocytes
Enzymes
Medium
See reference
Heart
Canine
Canine
Xi, Y. , Wu, G. , Ai, T. , Cheng, N. , Kalisnik, J. , Sun, J. , Abbasi, S. , Yang, D. , Fan, C. , Yuan, X. and Wang, S. et al.
Ionic Mechanisms Underlying the Effects of Vasoactive Intestinal Polypeptide on Canine Atrial Myocardium. ,
Circ Arrhythm Electrophysiol
 6 , 976-83 , 2013
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 300 u/ml
Protease : 0.03%
Medium
Tyrode's solution
Heart
Canine
Canine
Gan, T. , Qiao, W. , Xu, G. , Zhou, X. , Tang, B. , Song, J. , Li, Y. , Zhang, J. , Li, F. , Mao, T. and Jiang, T.
Aging-Associated Changes in L-type Calcium Channels in the Left Atria of Dogs. ,
Exp Ther Med
 6 , 919-924 , 2013
Cells
Atrial myocytes
Enzymes
Medium
Tyrode's solution
Heart
Canine
Canine
Harleton, E. , Besana, A. , Comas, G. , Danilo, P. , Rosen, T. , Argenziano, M. , Rosen, M. , Robinson, R. and Feinmark, S.
Ability to Induce Atrial Fibrillation in the Peri-Operative Period is Associated with Phosphorylation-Dependent Inhibition of TWIK Protein-Related Acid-Sensitive Potassium Channel 1 (TASK-1). ,
J Biol Chem
 288 , 2829-38 , 2013
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 60 u/ml
Protease XIV : 0.5 u/ml
Medium
See reference
Heart
Canine
Canine, adult
Calloe, K. , Nof, E. , Jespersen, T. , Di Diego, J. , Chlus, N. , Olesen, S.¸ Antzelevitch, C. and Cordeiro, J.
Comparison of the Effects of a Transient Outward Potassium Channel Activator on Currents Recorded from Atrial and Ventricular Cardiomyocytes. ,
J Cardiovasc Electrophysiol
 22 , 1057-66 , 2011
Cells
Cardiomyocytes
Enzymes
Protease Type XIV : 0.01%
Medium
HEPES
Heart
Canine
Canine, 20-25 kg
Burashnikov, A. , Zygmunt, A. , Di Diego, J. , Linhardt, G. , Carlsson, L. and Antzelevitch, C.
AZD1305 Exerts Atrial Predominant Electrophysiological Actions and is Effective in Suppressing Atrial Fibrillation and Preventing its Reinduction in the Dog. ,
J Cardiovasc Pharmacol
 56 , 80-90 , 2010
Cells
Cardiomyocytes
Enzymes
Medium
Tyrode's solution
Heart
Canine
Canine, adult
Gavi, S. , Yin, D. , Shumay, E. , Wang, H. and Malbon, C.
Insulin-Like Growth Factor-I Provokes Functional Antagonism and Internalization of Beta1-Adrenergic Receptors. ,
Endocrinology
 148 , 2653-62 , 2007
Cells
Cardiomyocytes
Enzymes
Protease : 0.008%
Medium
M199
Heart
Canine
Canine
Schotten, U. , de Haan, S. , Verheule, S. , Harks, E. , Frechen, D. , Bodewig, E. , Greiser, M. , Ram, R. , Maessen, J. , Kelm, M. , Allessie, M. and Van Wagoner, D.
Blockade of Atrial-Specific K+-currents Increases Atrial but not Ventricular Contractility by Enhancing Reverse Mode Na+/Ca2+-Exchange. ,
Cardiovasc Res
 73 , 37-47 , 2007
Cells
Atrial and ventricular myocytes
Enzymes
Medium
Tyrode's solution
Heart
Canine
Canine
Baba, S. , Dun, W. , Hirose, M. and Boyden, P.
Sodium Current Function in Adult and Aged Canine Atrial Cells. ,
Am J Physiol Heart Circ Physiol
 291 , H756-61 , 2006
Cells
Atrial myocytes
Enzymes
Medium
HEPES
Heart
Canine
Canine, 20-30 kg
Sun, H. , Chartier, D. , Leblanc, N. and Nattel, S.
Intracellular Calcium Changes and Tachycardia-Induced Contractile Dysfunction in Canine Atrial Myocytes. ,
Cardiovasc Res
 49 , 751-61 , 2001
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 110 u/ml
Medium
Tyrode's solution
Heart
Canine
Canine
Gintant, G.
Two Components of Delayed Rectifier Current in Canine Atrium and Ventricle. Does IKs Play a Role in the Reverse Rate Dependence of Class III Agents? ,
Circ Res
 78 , 26-37 , 1996
Cells
Ventricular and atrial myocytes
Enzymes
Medium
HEPES/Tyrode's
Heart
Canine
Dog, mongrel, adult
Spanier, A. and Weglicki, W.
Ca2+-Tolerant Adult Canine Myocytes: Preparation and Response to Anoxia/Acidosis ,
Am J Physiol
 243 , H448 , 1982
Cells
Myocytes
Enzymes
Hyaluronidase : 0.1%
Medium
Joklik's MEM
Heart
Mouse

Mouse, neonatal

Angelini, A. , Pi, X. and Xie, L.
Evaluation of Long-Chain Fatty Acid Respiration in Neonatal Mouse Cardiomyocytes using SeaHorse Instrument. ,
Star Protocols
 3 , 101392 , 2022
Cells
Cardiomyocytes
Enzymes
Trypsin : 0.05%
CLSPA : 150 u/ml
Medium
L-15
Heart
Mouse

Mouse, adult

Zhang, Z. , Zheng, W. , He, D. , Hu, Z. , Xie, Q. , Huang, M. , Li, W. and Huang, Z.
An Improved Procedure for Isolating Adult Mouse Cardiomyocytes for Epicardial Activation Mapping. ,
J Cell Mol Med
 25 , 11257-11263 , 2021
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 35-50 u/ml
Protease : 0.005%
Medium
Tyrode solution
Heart
Mouse
Mouse
Hu, D. , Yin, C. , Mohanta, S. , Weber, C. and Habenicht, A.
Prepartaion of Single Cell Suspensions from Mouse Aorta ,
Bio Protoc
 6 , 11 , 2016
Cells
Aortic
Enzymes
Collagenase Type 1 : 400 u/ml
Collagenase : 120 u/ml
Hyaluronidase : 60 u/ml
Medium
DPBS
Heart
Mouse
Mouse, male, 12-18 week
Jian, Z, Chen, Y, Shimkunas, R, Jian, Y, Jaradeh, M, Chavez, K, Chiamvimonvat, N, Tardiff, J, Izu, L, Ross, R. and Chen-Izu, Y
In Vivo Cannulation Methods for Cardiomyocytes Isolation from Heart Disease Models. ,
PLoS ONE
 11 , e0160605 , 2016
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 300 u/ml
Protease XIV : 0.004%
Medium
Perfusion solution (see reference)
Heart
Mouse
Mouse, 4-6 week
Xu, J. , Lee, Y. , Ran, X. , Liao, S. , Yang, J. , Au, K. , Lai, W. , Esteban, M. and Tse, H.
Generation of Induced Cardiospheres via Reprogramming of Skin Fibroblasts for Myocardial Regeneration. ,
Stem Cells
 34 , 2693-2706 , 2016
Cells
Cardiospheres
Enzymes
Medium
PBS
Heart
Mouse

Mouse, 8-12 week

Ackers-Johnson, M. , Li, P. , Holmes, A. , O'Brien, S. , Pavlovic, D. and Foo, R.
A Simplified, Langendorff-Free Method for Concomitant Isolation of Viable Cardiac Myocytes and Nonmyocytes From the Adult Mouse Heart. ,
Circ Res
 119 , 909-20 , 2016
Cells
Cardiomyocytes
Enzymes
Protease XIV : 0.005%
Medium
Perfusion buffer (see reference)
Heart
Mouse
Mouse, 15-30 gm
Henn, M. , Janjua, M. , Kanter, E. , Makepeace, C. , Schuessler, R. , Nichols, C. and Lawton, J.
Adenosine Triphosphate-Sensitive Potassium Channel Kir Subunits Implicated in Cardioprotection by Diazoxide. ,
J Am Heart Assoc
 4 , 2015
Cells
Ventricular myocytes
Enzymes
Medium
see reference
Heart
Mouse
Mouse, adult
Santos Nascimento, D, Mosqueira, D, Sousa, L, Teixeira, M, Filipe, M, Resende, T, Araujo, A, Valente, M, Almeida, J, Martins, J, Santos, J, Barcia, R, Cruz, P, Cruz, H. and Pinto-d-O, P
Human Umbilical Cord Tissue-Derived Mesenchymal Stromal Cells Attenuate Remodeling after Myocardial Infarction by Proangiogenic, Antiapoptotic, and Endogenous Cell-Activation Mechanisms. ,
Stem Cell Res Ther
 5 , 5 , 2014
Cells
Cardiac Progenitor
Enzymes
Medium
MEM
Heart
Mouse
Mouse, 3-4 week
Wang, H. , Chen, H. , Feng, B. , Wang, X. , He, X. , Hu, R. , Yin, M. , Wang, W. , Fu, W. and Xu, Z.
Isolation and Characterization of a Sca-1+/CD31- Progenitor Cell Lineage Derived From Mouse Heart Tissue. ,
BMC Biotechnol
 14 , 75 , 2014
Cells
Cardiac progenitor
Enzymes
Medium
DMEM
Heart
Mouse
Mouse
Zafiriou, M. , Noack, C. , Unsold, B. , Didie, M. , Pavlova, E. , Fischer, H. , Reichardt, H. , Bergmann, M. , El-Armouche, A. , Zimmermann, W. and Zelarayan, L.
Erythropoietin Responsive Cardiomyogenic Cells Contribute to Heart Repair Post Myocardial Infarction. ,
Stem Cells
 32 , 2480 , 2014
Cells
Cardiac progenitor
Enzymes
Medium
HBSS
Heart
Mouse
Mouse
Touchberry, C. , Green, T. , Tchikrizov, V. , Mannix, J. , Mao, T. , Carney, B. , Girgis, M. , Vincent, R. , Wetmore, L. , Dawn, B. , Bonewald, L. , Stubbs, J. and Wacker, M.
FGF23 is a Novel Regulator of Intracellular Calcium and Cardiac Contractility in Addition to Cardiac Hypertrophy. ,
Am J Physiol Endocrinol Metab
 304 , E863 , 2013
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 360 u/ml
Papain : 0.4 u/ml
Medium
L-15
Heart
Mouse
Mouse, adult
Kohncke, C. Lisewski, U. , Schleussner, L. , Gaertner, C. , Reichert, S. and Roepke, T.
Isolation and Kv Channel Recordings in Murine Atrial and Ventricular Cardiomyocytes. ,
J Vis Exp
 73 , e50145 , 2013
Cells
Cardiomyocytes
Enzymes
Medium
see reference
Heart
Mouse
Mouse
Hennessey, J. , Wei, E. and Pitt, G.
Fibroblast Growth Factor Homologous Factors Modulate Cardiac Calcium Channels. ,
Circ Res
 113 , 381-8 , 2013
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 150 u/ml
Medium
MEM
Heart
Mouse
Mouse
Pratumvinit, B. , Reesukumal, K. , Janebodin, K. , Ieronimakis, N. and Reyes, M.
Isolation, Characterization, and Transplantation of Cardiac Endothelial Cells. ,
Biomed Res Int
 2013 , 359412 , 2013
Cells
Cardiac endothelial
Enzymes
Medium
PBS
Heart
Mouse
Mouse, 2-6 month
Christel, C. , Cardona, N. , Mesirca, P. , Herrmann, S. , Hofmann, F. , Striessnig, J. , Ludwig, A. , Mangoni, M. and Lee, A.
Distinct Localization and Modulation of Cav1.2 and Cav1.3 L-type Ca2+ Channels in Mouse Sinoatrial Node. ,
J Physiol
 590 , 6327 , 2012
Cells
Sinoatrial node
Enzymes
Collagenase Type 2 : 229 u/ml
Elastase : 1.9 u/ml
Protease Type XIV : 0.9 u/ml
Medium
Tyrode's solution
Heart
Mouse
Mouse, adult
Flynn, J. , Santana, L. and Melov, S.
Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes. ,
J Vis Exp
 58 , e3302 , 2011
Cells
Cardiomyocytes
Enzymes
Collagenase Type 1 : 0.1-0.4%
Protease Type XIV : 0.004-.02%
Medium
see reference
Heart
Mouse
Mouse, male, 8-25 week
Carley, A. and Kleinfeld, A.
Fatty Acid (FFA) Transport in Cardiomyocytes Revealed by Imaging Unbound FFA is Mediated by an FFA Pump Modulated by the CD36 Protein. ,
J Biol Chem
 286 , 4589-97 , 2011
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 59 u/ml
Medium
MEM
Heart
Mouse
Mouse
Butcher, M. , Herre, M. , Ley, K. and Galkina, E.
Flow Cytometry Analysis of Immune Cells Within Murine Aortas. ,
J Vis Exp
 53 , e2828 , 2011
Cells
Aortic adventitial leukocytes
Enzymes
Collagenase Type 2 : 300 u/ml
Elastase : 5.6 u/ml
Medium
PBS
Heart
Mouse
Mouse, male, 6 week
Makino, A. , Suarez, J. , Wang, H. , Belke, D. , Scott, B. and Dillmann, W.
Thyroid Hormone Receptor-beta is Associated With Coronary Angiogenesis During Pathological Cardiac Hypertrophy. ,
Endocrinology
 150 , 2008 , 2009
Cells
Coronary endothelial cells
Enzymes
Medium
M199
Heart
Mouse
Mouse, adult
Zhang, Y, Kanter, E. , Laing, J. , Aprhys, C. , Johns, D. , Kardami, E. and Yamada, K.
Connexin43 Expression Levels Influence Intercellular Coupling and Cell Proliferation of Native Murine Cardiac Fibroblasts. ,
Cell Commun Adhes
 15 , 289 , 2008
Cells
Ventricular myoctes
Enzymes
Collagenase : 0.1%
Medium
M199
Heart
Mouse
Mouse, adult, 9-13 week
Kabaeva, Z. , Zhao, M. and Michele, D.
Blebbistatin Extends Culture Life of Adult Mouse Cardiac Myocytes and Allows Efficient and Stable Transgene Expression. ,
Am J Physiol Heart Circ Physiol
 294 , H1667-74 , 2008
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 620 u/ml
Protease XIV : 0.104 u/ml
Medium
Myocyte buffer see reference

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.