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Tissue References Search Tool

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Heart
Canine
Canine
Xi, Y. , Wu, G. , Ai, T. , Cheng, N. , Kalisnik, J. , Sun, J. , Abbasi, S. , Yang, D. , Fan, C. , Yuan, X. and Wang, S. et al.
Ionic Mechanisms Underlying the Effects of Vasoactive Intestinal Polypeptide on Canine Atrial Myocardium. ,
Circ Arrhythm Electrophysiol
 6 , 976-83 , 2013
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 300 u/ml
Protease : 0.03%
Medium
Tyrode's solution
Heart
Canine
Canine
Gan, T. , Qiao, W. , Xu, G. , Zhou, X. , Tang, B. , Song, J. , Li, Y. , Zhang, J. , Li, F. , Mao, T. and Jiang, T.
Aging-Associated Changes in L-type Calcium Channels in the Left Atria of Dogs. ,
Exp Ther Med
 6 , 919-924 , 2013
Cells
Atrial myocytes
Enzymes
Medium
Tyrode's solution
Heart
Canine
Canine
Harleton, E. , Besana, A. , Comas, G. , Danilo, P. , Rosen, T. , Argenziano, M. , Rosen, M. , Robinson, R. and Feinmark, S.
Ability to Induce Atrial Fibrillation in the Peri-Operative Period is Associated with Phosphorylation-Dependent Inhibition of TWIK Protein-Related Acid-Sensitive Potassium Channel 1 (TASK-1). ,
J Biol Chem
 288 , 2829-38 , 2013
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 60 u/ml
Protease XIV : 0.5 u/ml
Medium
See reference
Heart
Canine
Canine, adult
Calloe, K. , Nof, E. , Jespersen, T. , Di Diego, J. , Chlus, N. , Olesen, S.¸ Antzelevitch, C. and Cordeiro, J.
Comparison of the Effects of a Transient Outward Potassium Channel Activator on Currents Recorded from Atrial and Ventricular Cardiomyocytes. ,
J Cardiovasc Electrophysiol
 22 , 1057-66 , 2011
Cells
Cardiomyocytes
Enzymes
Protease Type XIV : 0.01%
Medium
HEPES
Heart
Canine
Canine, 20-25 kg
Burashnikov, A. , Zygmunt, A. , Di Diego, J. , Linhardt, G. , Carlsson, L. and Antzelevitch, C.
AZD1305 Exerts Atrial Predominant Electrophysiological Actions and is Effective in Suppressing Atrial Fibrillation and Preventing its Reinduction in the Dog. ,
J Cardiovasc Pharmacol
 56 , 80-90 , 2010
Cells
Cardiomyocytes
Enzymes
Medium
Tyrode's solution
Heart
Canine
Canine, adult
Gavi, S. , Yin, D. , Shumay, E. , Wang, H. and Malbon, C.
Insulin-Like Growth Factor-I Provokes Functional Antagonism and Internalization of Beta1-Adrenergic Receptors. ,
Endocrinology
 148 , 2653-62 , 2007
Cells
Cardiomyocytes
Enzymes
Protease : 0.008%
Medium
M199
Heart
Canine
Canine
Schotten, U. , de Haan, S. , Verheule, S. , Harks, E. , Frechen, D. , Bodewig, E. , Greiser, M. , Ram, R. , Maessen, J. , Kelm, M. , Allessie, M. and Van Wagoner, D.
Blockade of Atrial-Specific K+-currents Increases Atrial but not Ventricular Contractility by Enhancing Reverse Mode Na+/Ca2+-Exchange. ,
Cardiovasc Res
 73 , 37-47 , 2007
Cells
Atrial and ventricular myocytes
Enzymes
Medium
Tyrode's solution
Heart
Canine
Canine
Baba, S. , Dun, W. , Hirose, M. and Boyden, P.
Sodium Current Function in Adult and Aged Canine Atrial Cells. ,
Am J Physiol Heart Circ Physiol
 291 , H756-61 , 2006
Cells
Atrial myocytes
Enzymes
Medium
HEPES
Heart
Canine
Canine, 20-30 kg
Sun, H. , Chartier, D. , Leblanc, N. and Nattel, S.
Intracellular Calcium Changes and Tachycardia-Induced Contractile Dysfunction in Canine Atrial Myocytes. ,
Cardiovasc Res
 49 , 751-61 , 2001
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 110 u/ml
Medium
Tyrode's solution
Heart
Canine
Canine
Gintant, G.
Two Components of Delayed Rectifier Current in Canine Atrium and Ventricle. Does IKs Play a Role in the Reverse Rate Dependence of Class III Agents? ,
Circ Res
 78 , 26-37 , 1996
Cells
Ventricular and atrial myocytes
Enzymes
Medium
HEPES/Tyrode's
Heart
Canine
Dog, mongrel, adult
Spanier, A. and Weglicki, W.
Ca2+-Tolerant Adult Canine Myocytes: Preparation and Response to Anoxia/Acidosis ,
Am J Physiol
 243 , H448 , 1982
Cells
Myocytes
Enzymes
Hyaluronidase : 0.1%
Medium
Joklik's MEM
Heart
Porcine
Porcine
Butcher, J. and Nerem, R.
Porcine Aortic Valve Interstitial Cells in Three-Dimensional Culture: Comparison of Phenotype with Aortic Smooth Muscle Cells. ,
J Heart Valve Dis
 13 , 478 , 2004
Cells
Aortic smooth muscle and intersitial cells
Enzymes
Collagenase : 600 u/ml
Medium
PBS
Heart
Porcine
Porcine
Christ, M. , Gunther, A. , Heck, M. , Schmidt, B. , Falkenstein, E. and Wehling, M.
Aldosterone, Not Estradiol, Is the Physiological Agonist for Rapid Increases in cAMP in Vascular Smooth Muscle Cells ,
Circulation
 99 , 1485 , 1999
Cells
Coronary vascular smooth muscle (PCVSMCs)
Enzymes
Trypsin : 0.037%
Medium
HEPES
Heart
Porcine
Miniature swine, adult, female, 25-40 kg, familiarized with treadmill exercise
Bowles, D. , Hu, Q. , Laughlin, M. and Sturek, M.
Exercise Training Increases L-type Calcium Current Density in Coronary Smooth Muscle ,
Am J Physiol
 275 (44) , H2159 , 1998
Cells
Coronary smooth muscle
Enzymes
Trypsin : 0.1%
Medium
Krebs bicarbonate solution
Intestine
Canine
Dog, adult
Damholt AB, Buchan AM, Kofod H.
Glucagon-like-peptide-1 secretion from canine L-cells is increased by glucose-dependent-insulinotropic peptide but unaffected by glucose ,
Endocrinology
 139 , 2085-91 , 1998
Cells
Intestinal L-cells
Enzymes
Collagenase Type 1 : 75 u/ml
Medium
HBSS
Kidney
Canine
Canine
Basu, J. , Genheimer, C. , Sangha, N. , Quinlan, S. , Guthrie, K. , Kelley, R. , Ilagan, R. , Jain, D. , Bertram, T. and Ludlow, J.
Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney. ,
Lipids Health Dis
 10 , 171 , 2011
Cells
Renal adipose derived cells
Enzymes
Medium
DMEM
Kidney
Canine
Dog, 1-5 months
States, B. , Reynolds, R. , Lee, J. and Segal, S.
Cystine Uptake By Cultured Cells Originating From Dog Proximal Tubule Segments ,
In Vitro Cell Dev Biol
 26 , 105 , 1990
Cells
Proximal tubules
Enzymes
Medium
Krebs Ringer bicarbonate buffer
Kidney
Canine
Dog, mongrel, 20 Kg
Yau, C. , Rao, L. and Silverman, M.
Sugar Uptake Into a Primary Culture of Dog Kidney Proximal Tubular Cells ,
Can J Physiol Pharmacol
 63 , 417 , 1985
Cells
Proximal tubular
Enzymes
Medium
(see reference)
Kidney
Porcine
Porcine, hybrid
Heussner, A. and Dietrich, D.
Primary Porcine Proximal Tubular Cells as an Alternative to Human Primary Renal Cells in Vitro: an Initial Characterization. ,
BMC Cell Biol
 14 , 55 , 2013
Cells
Kidney
Enzymes
Medium
HBSS
Liver
Canine
Dog, 13 years old
(also rat, guinea-pig, rabbit, human)
Reese, J. and Byard, J.
Isolation And Culture of Adult Hepatocytes from Liver Biopsies ,
In Vitro
 17 , 935 , 1981
Cells
Hepatocytes
Enzymes
Collagenase : 90 u/ml
Medium
CF EGTA perfusate
Liver
Canine
Dog, adult
Vickrey, H. , Ramon, J. and McCann, D.
Continuous Culture of Normal Adult Mammalian Hepatocytes Exhibiting Parenchymal Functions ,
In Vitro
 15 , 120 , 1979
Cells
Hepatocytes
Enzymes
Trypsin : 0.1%
Medium
HBSS, CMF
Liver
Porcine

Porcine

Almalla, A. , Elomaa, L. , Bechtella, L. , Daneshgar, A. , Yavvari, P. and Mahfouz, Z.
Papain-Based Solubilization of Decellularized Extracellular Matrix for the Preparation of Bioactive, Thermosensitive Pregels. , Biomacromolecules Vol. 24 , 5620-5637 , 2023
Cells
Decellularized Extracellular Matrix
Enzymes
Papain : 0.05%
Medium
PBS
Liver
Porcine
Porcine, mini-pig, 13 kg
Meng, F. , Chen, Z. , Han, M. , Hu, X. , He, X. , Liu, Y. , He, W. , Huang, W. , Guo, H. and Zhou, P.
Porcine Hepatocyte Isolation and Reversible Immortalization Mediated by Retroviral Transfer and Site-Specific Recombination. ,
World J Gastroenterol
 16 , 1660 , 2010
Cells
Hepatocytes
Enzymes
Deoxyribonuclease I : see reference
Medium
Williams E
Liver
Porcine
Porcine, Yorkshire, male, 21 day
Terner, M. , Gilmore, W.J. , Lou, Y. and Squires, E.J.
The Role of CYP2A and CYP2E1 in the Metabolism of 3-Methylindole in Primary Cultured Porcine Hepatocytes. ,
Drug Metab Dispos
 34 , 848 , 2006
Cells
Hepatocytes
Enzymes
Medium
Williams E
Liver
Porcine
Porcine, Chinese mini pig, 6-10 kg
Li, J, Li, L. , Chao, H. , Yang, Q. , Liu, X. , Sheng, J. , Yu, H. and Huang, J
Isolation and Short Term Cultivation of Swine Hepatocytes for Bioartificial Liver Support System. ,
Hepatobiliary Pancreat Dis Int
 4 , 249 , 2005
Cells
Hepatocytes
Enzymes
Collagenase Type 4 : 125 u/ml
Medium
Williams E
Liver
Porcine
Porcine, male, 2-3 week
Raman Priya, Donkin Shawn S, Spurlock Michael E.
Regulation of hepatic glucose metabolism by leptin in pig and rat primary hepatocyte cultures ,
Am J Physiol Regul Integr Comp Physiol
 286 , R206-16 , 2004
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
DMEM
Liver
Porcine
Porcine, one week
Wang Y. , Liu H. , Guo H. , Wen H. and Liu J.
Primary Hepatocyte Culture in Collagen Gel Mixture and Collagen Sandwich ,
World J Gastroenterol
 10 , 699 , 2004
Cells
Hepatocytes
Enzymes
Medium
RPMI 1640
Liver
Porcine
Porcine, adult
Zhou X, Liu L, Kano J, Mukaiyama T. and Tokiwa T.
Isolation and Cultivation of Porcine Hepatocytes for Extracorporeal Artificial Liver Support System ,
Chin Med J
 114 , 946 , 2001
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Medium
MEM
Liver
Porcine
Porcine, male, 6-40 kg
Gerlach, J. , Brombacher, J. , Smith, M. and Neuhaus, P.
High Yield Hepatocyte Isolation from Pig Livers for Investigation of Hybrid Liver Support Systems: Influence of Collagenase Concentration and Body Weight ,
J Surg Res
 62 (1) , 85 , 1996
Cells
Hepatocytes
Enzymes
Collagenase : 0.8%
Medium
PBS
Lung
Porcine
Swine fetuses (70 day), adult Amer Dutch 250 day
(also human fetuses, 80 day)
Hinz, R. and Syverton, J.
Mammalian Cell Cultures for Study of Influenza Virus. I. Preparation of Monolayer Cultures with Collagenase ,
Proc Soc Exp Biol Med
 101 , 19 , 1959
Cells
Lung
Enzymes
Collagenase : 0.01%
Medium
HBSS
Mammary
Canine

Canine, adult

Petrouškova, P. , Hudáková, N. , Almášiová, V. , Valenčáková, A. , Horňáková, L. , Huniadi, M. and Čížková, D.
Establishment of Primary Cell Cultures from Canine Mammary Gland Malignant Tumours: A Preliminary Study. , J Vet Res Vol. 69 , 159-168 , 2025
Cells
Mammary gland tumor
Enzymes
Medium
EBSS
Miscellaneous
Canine

Canine, Rat, Rabbit, Ovine, Bovine, Human

Basatvat, S. , Bach, F. , Barcellona, M. , Binch, A. , Buckley, C. , Bueno, B. , Chahine, N. , Chee, A. and Maitre, C.
Harmonization and Standardization of Nucleus Pulposus Cell Extraction and Culture Methods. ,
Spine
 6 , e1238 , 2023
Cells
nucleus pulposus
Enzymes
Pronase : 0.075%
Medium
DMEM
Miscellaneous
Porcine
Porcine, 6 month
Kean, T. and Dennis, J.
Synoviocyte Derived-Extracellular Matrix Enhances Human Articular Chondrocyte Proliferation and Maintains Re-Differentiation Capacity at Both Low and Atmospheric Oxygen Tensions. ,
PLoS ONE
 10 , e0129961 , 2015
Cells
Synoviocytes
Enzymes
Hyaluronidase : 660 u/ml
Trypsin : 0.25%
Collagenase Type 2 : 583 u/ml
Medium
DMEM
Miscellaneous
Porcine
Porcine, <10 week
Potier, E. and Ito. K.
Can Notochordal Cells Promote Bone Marrow Stromal Cell Potential for Nucleus Pulposus Enrichment? A Simplified in Vitro System. ,
Tissue Eng Part A
 20 , 3241-51 , 2014
Cells
Nucleus pulposus
Enzymes
Collagenase : 0.05%
Pronase : 0.3%
Medium
DMEM
Miscellaneous
Porcine
Porcine, various ages
Cho, H. , Park, S. , Lee, S. , Kang, M. , Hasty, K. and Kim, S.
Snapshot of Degenerative Aging of Porcine Intervertebral Disc: A Model to Unravel the Molecular Mechanisms. ,
Exp Mol Med
 43 , 334-40 , 2011
Cells
Nucleous pulposus and AF
Enzymes
Pronase : 0.05%
Collagenase : 0.2%
Medium
Modified F-12K
Muscle
Canine
Canine
Parker, M. , Loretz, C. , Tyler, A. , Snider, L. , Storb. R. and Tapscott, S.
Inhibition of CD26/DPP-IV Enhances Donor Muscle Cell Engraftment and Stimulates Sustained Donor Cell Proliferation. ,
Skelet Muscle
 2 , 4 , 2012
Cells
Skeletal muscle
Enzymes
Collagenase Type 4 : 200 u/ml
Neutral Protease : 1 u/ml
Medium
DMEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.