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Tissue References Search Tool

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Lung
Human
Human, 16-24 week therapeutic abortions
Liley, H. , Ertsey, R. , Gonzales, L. , Odom, M. , Hawgood, S. , Dobbs, L. and Ballard, P.
Synthesis of Surfactant Components by Cultured Type II Cells From Human Lung ,
Biochim Biophys Acta
 961 , 86 , 1988
Cells
Alveolar type II
Fetal
Enzymes
Trypsin : 50 ug/ml
Medium
Ham's F-12, Eagle's MEM
Lung
Human
Human
Lechner, J. , Haugen, A. , McClendon, I. and Pettis, E.
Clonal Growth of Normal Adult Human Bronchial Epithelial Cells in a Serum-Free Medium ,
In Vitro
 18 (7) , 633 , 1982
Cells
Epithelial
Enzymes
Trypsin : 0.02%
Medium
Medium 199
Lung
Human
Human, 12-16 wk old embryo
Kan, M. and Yamane, I.
In Vitro Proliferation and Lifespan of Human Diploid Fibroblasts in Serum-Free BSA-Containing Medium ,
J Cell Physiol
 111 , 155 , 1982
Cells
Fibroblasts
Enzymes
Trypsin : 0.01%
Medium
Eagle's MEM
Lung
Human
Human fetuses, 80 day
(also swine fetuses, 70 day, adult Amer Dutch, 250 day)
Hinz, R. and Syverton, J.
Mammalian Cell Cultures for Study of Influenza Virus. I. Preparation of Monolayer Cultures with Collagenase ,
Proc Soc Exp Biol Med
 101 , 19 , 1959
Cells
Lung
Enzymes
Collagenase : 0.01%
Medium
HBSS
Mammary
Human
Human
Labarge, M. , Garbe, J. and Stampfer, M.
Processing of Human Reduction Mammoplasty and Mastectomy Tissues for Cell Culture. ,
J Vis Exp
 71 , 2013
Cells
Mammary epithelial
Enzymes
Collagenase : 200 u/ml
Hyaluronidase : 100 u/ml
Medium
DMEM/F-12
Mammary
Human
Human, normal biopsy
Ronnov-Jessen L. , Villadsen R. , Edwards J. and Petersen O.
Differential Expression of a Chloride Intracellular Channel Gene, CLIC4, in Transforming Growth Factor-beta1-mediated Conversion of Fibroblasts to Myofibroblasts ,
Am J Pathol
 161 , 471 , 2002
Cells
Fibroblasts
Enzymes
Collagenase Type 3 : 900 u/ml
Medium
DMEM/F-12
Mammary
Human
Human
Huss, F. and Kratz, G.
Mammary Epithelial Cell and Adipocyte Co-Culture in a 3-D Matrix: The First Step Towards Tissue-Engineered Human Breast Tissue. ,
Cells Tissues Organs
 169 , 361-7 , 2001
Cells
Mammary epithelial cells
Enzymes
Medium
DMEM
Mammary
Human
Human
Ogmundsdottir, H. , Petursdottir, I. , Gudmundsdottir, I. , Amundadottir, L. , Ronnov-Jessen, L. and Petersen, O.
Effects of Lymphocytes and Fibroblasts on the Growth of Human Mammary Carcinoma Cells Studied in Short-Term Primary Cultures ,
In Vitro Cell Dev Biol
 29A , 936 , 1993
Cells
Epithelial
Fibroblasts
Enzymes
Collagenase Type 1 : 450 IU/ml
Medium
DMEM/Ham's F-12
Mammary
Human
Human
Ronnov-Jessen, L. , VanDeurs, B. , Nielsen, M. and Petersen, O.W.
Identification, Paracrine Generation, and Possible Function of Human Breast Carcinoma Myofibroblasts in Culture ,
In Vitro Cell Dev Biol
 28 , 273 , 1992
Cells
Myofibroblasts
Enzymes
Collagenase : 900 IU/ml
Medium
DME - F12
Mammary
Human
Human, female, 15-61 yrs old
Berthon, P. , Pancino, G. , Cremoux, P. , Roseto, A. , Gespach, C. and Calvo, F.
Characterization of Normal Breast Epithelial Cells in Primary Cultures: Differentiation and Growth Factor Receptors Studies ,
J Tiss Cul Meth
 28A , 716 , 1992
Cells
Epithelial
Enzymes
Hyaluronidase : 150 IU/ml
Medium
DMEM
Mammary
Human
Human
Emerman, J. and Wilkinson, D.
Routine Culturing of Normal, Dysplastic and Malignant Human Mammary Epithelial Cells from Small Tissue Samples ,
In Vitro Cell Dev Biol
 26 , 1186 , 1990
Cells
Epithelial
Enzymes
Collagenase : 2.0%
Medium
DMEM/Ham's F-12
Mammary
Human
Human
Leung, C. and Shiu, R.
Morphological and Proliferative Characteristics of Human Breast Tumor Cells Cultured on Plastic and in Collagen Matrix ,
In Vitro
 18 , 476 , 1981
Cells
Tumor, breast
Enzymes
Neuraminidase : 0.8 u/ml
Medium
HBSS
Mammary
Human
Human
Stampfer, M. , Hallowes, R. and Hackett, A.
Growth of Normal Human Mammary Cells in Culture ,
In Vitro
 16 (5) , 415 , 1980
Cells
Epithelial
Enzymes
Hyaluronidase : 100 u/ml
Medium
DMEM/Ham's F-12
Miscellaneous
Human

Human, 18-30 years

Pardo-Rodríguez, B. , Baraibar, A. , Manero-Roig, I. , Luzuriaga, J. , Salvador-Moya, J. , Polo, Y. , Basanta-Torres, R. , Unda, F. , Mato, S. , Ibarretxe, G. and Pineda, J.
Functional Differentiation of Human Dental Pulp Stem Cells into Neuron-like Cells Exhibiting Electrophysiological Activity. ,
Stem Cell Res Ther
 16 , 10 , 2025
Cells
Dental pulp stem cells
Enzymes
Medium
DMEM
Miscellaneous
Human

Human

Xue, Y. , Seiler, M. , Tang, W. , Wang, J. , Delgado, J. , McLelland, B. , Nistor, G. , Keirstead, H. and Browne, A.
Retinal Organoids On-a-Chip: a Micro-Millifluidic Bioreactor for Long-term Organoid Maintenance. ,
Lab Chip
 21 , 3361-3377 , 2021
Cells
Stem cell-derived retinal organoids
Enzymes
PDS kit : see reference
Medium
EBSS
Miscellaneous
Human
Human
Foster, D. , Marshall, C. , Gulati, G. , Chinta, M. , Nguyen, A. , Salhotra, A. , Jones, R. , Burcham, A. , Lerbs, T. , Cui, L. , King, M. , Titan, A. , Ransom, R. , Manjunath, A. , Hu, M. and Blackshear, C.
Elucidating the Fundamental Fibrotic Processes Driving Abdominal Adhesion Formation. ,
Nat Commun
 11 , 4061 , 2020
Cells
Adhesion fibroblasts
Enzymes
Medium
Medium 199
Miscellaneous
Human

Human

Yoon, S. , Elahi, L. , Pașca, A. , Marton, R. , Gordon, A. , Revah, O. , Miura, Y. , Walczak, E. , Holdgate, G. , Fan, H. , Huguenard, J. , Geschwind, D. and Pașca, S.
Reliability of Human Cortical Organoid Generation. ,
Nat Methods
 16 , 75-78 , 2019
Cells
Cortical spheroids
Enzymes
Papain : 40 u/ml
Medium
EBSS
Miscellaneous
Human

Human

Velasco, S. , Kedaigle, A. , Simmons, S. , Nash, A. , Rocha, M. , Quadrato, G. , Paulsen, B. , Nguyen, L. , Adiconis, X. , Regev, A. , Levin, J. and Arlotta, P.
Individual Brain Organoids Reproducibly Form Cell Diversity of the Human Cerebral Cortex. ,
Nature
 570 , 523-527 , 2019
Cells
Brain organoids
Enzymes
PDS kit : see reference
Medium
PBS
Miscellaneous
Human
Human
Sun, Y. , Lv, M. , Zhou, L. , Tam, V. , Lv, F. , Chan, D. , Wang, H. , Zheng, Z. , Cheung, K. and Leung, V.
Enrichment of Committed Human Nucleus Pulposus Cells Expressing Chondroitin Sulfate Proteoglycans under Alginate Encapsulation. ,
Osteoarthritis Cartilage
 23 , 1194-203 , 2015
Cells
Nucleus pulposus
Enzymes
Pronase : 0.25%
Collagenase Type 2 : 600 u/ml
Medium
DMEM
Miscellaneous
Human
Human, 3-7 year
Salmon, B. , Bardet, C, Khaddam, M. Naji, J. , Coyac, B. , Baroukh, B. , Letourneur, F. , Lesieur, J. , Decup, F. , Le Denmat, D. , Nicoletti, A. , Poliard, A, Rowe, P. , Huet, E. , Vital, S. and Linglart, A.
MEPE-Derived ASARM Peptide Inhibits Odontogenic Differentiation of Dental Pulp Stem Cells and Impairs Mineralization in Tooth Models of X-linked Hypophosphatemia. ,
PLoS ONE
 8 , e56749 , 2013
Cells
Dental pulp stem cells
Enzymes
Medium
DMEM
Miscellaneous
Human
Human
Bonnamain, V. , Thinard, R. , Sergent-Tanguy, S. , Huet, P. , Bienvenu, G. , Naveilhan, P. , Farges, J. and Alliot-Licht, B.
Human Dental Pulp Stem Cells Cultured in Serum-Free Supplemented Medium. ,
Front Physiol
 4 , 357 , 2013
Cells
Dental pulp stem
Enzymes
Medium
DMEM/F12
Miscellaneous
Human
Human
Lee, Y. , Lee, J. , Park, H. , Lim, Y. , Lee, J. , Wang, S. and Lee, B.
Isolation of Mesenchymal Stromal Cells (MSCs) from Human Adenoid Tissue. ,
Cell Physiol Biochem
 31 , 513-24 , 2013
Cells
Mesenchymal stromal
Enzymes
Medium
PBS
Miscellaneous
Human
Human
Ozdener, H. , Spielman, A. and Rawson, N.
Isolation and Culture of Human Fungiform Taste Papillae Cells ,
J Vis Exp
 63 , e3730 , 2012
Cells
Fungiform taste papillae
Enzymes
Medium
see reference
Miscellaneous
Human
Human
Pauly, S. , Klatte, F. , Strobel, C. , Schmidmaier, G. , Greiner, S. and Scheibel, M. Wildemann B
Characterization of Tendon Cell Cultures of the Human Rotator Cuff. ,
Eur Cell Mater
 20 , 84-97 , 2010
Cells
Tendon cells
Enzymes
Medium
DMEM/Hams F12
Miscellaneous
Human
Human
Panetta, N. , Gupta, D. , Kwan, M. , Wan, D. , Commons, G. and Longaker, M.
Tissue Harvest by Means of Suction-Assisted or Third-Generation Ultrasound-Assisted Lipoaspiration Has No Effect on Osteogenic Potential of Human Adipose-Derived Stromal Cells. ,
Plast Reconstr Surg
 124 , 65 , 2009
Cells
Adipose derived stromal
Enzymes
Medium
DMEM
Miscellaneous
Human
Human
Nakamura, T. , Endo, K. and Kinoshita, S.
Identification of Human Oral Keratinocyte Stem/Progenitor Cells by Neurotrophin Receptor p75 and the Role of Neurotrophin/p75 Signaling. ,
Stem Cells
 25 , 628-38 , 2007
Cells
Oral epithelial
Enzymes
Neutral Protease : 1.2 u/ml
Trypsin : 0.05%
Medium
PBS
Miscellaneous
Human
Human
Patino WillmarD, Kang Ju-Gyeong, Matoba Satoaki, Mian OmarY, Gochuico BernadetteR, Hwang PaulM.
Atherosclerotic plaque macrophage transcriptional regulators are expressed in blood and modulated by tristetraprolin ,
Circ Res
 98 , 1282-9 , 2006
Cells
Carotid artery plaque macrophage
Enzymes
Medium
HBSS
Miscellaneous
Human
Human
Rafiee, P. , Ogawa, H. , Heidemann, J. , Li, M. , Aslam, M. , Lamirand, T. , Fisher, P. , Graewin, S. , Dwinell, M. , Johnson, C. , Shaker, R. and Binion, D.
Isolation and Characterization of Human Esophageal Microvascular Endothelial Cells: Mechanisms of Inflammatory Activation. ,
Am J Physiol Gastrointest Liver Physiol
 285 , G1277 , 2003
Cells
Esophageal microvascular endothelial
Enzymes
Medium
MCDB-131
Miscellaneous
Human
Smoot, D. , Sewchand, J. , Young, K. , Desbrodes, B. , Allen, C. and Naab, T.
A Method for Establishing Primary Cultures of Human Gastric Epithelial Cells ,
Meth Cell Sci
 22 , 133 , 2000
Cells
Gastric epithelial cells
Enzymes
Medium
L-15
Miscellaneous
Human
Human
Chen V, Croft D, Purkis P, Kramer IM.
Co-culture of synovial fibroblasts and differentiated U937 cells is sufficient for high interleukin-6 but not interleukin-1beta or tumour necrosis factor-alpha release. ,
Br J Rheumatol
 37 , 148-56 , 1998
Cells
Synoviocytes
Enzymes
Collagenase : 0.2%
Medium
DMEM/F12
Miscellaneous
Human
Human
Lanas. A. , Nerin, J. , Esteva, F. and Sainz, R.
Non-Steroidal Anti-Inflammatory Drugs and Prostaglandin Effects on Pepsinogen Secretion by Dispersed Human Peptic Cells. ,
Gut
 36 , 657-63 , 1995
Cells
Peptic cells
Enzymes
Medium
Ringer solution
Miscellaneous
Human
Human
Stern MH, Dreizen S, Mackler BF, Levy BM.
Isolation and characterization of inflammatory cells from the human periapical granuloma ,
J Dent Res
 61 , 1408-12 , 1982
Cells
Periapical granuloma
Enzymes
CLSPA : 0.25%
Medium
RPMI-1640
Muscle
Chicken
Chicken, 1-2 day
Dirksen W. , Vladic F. and Fisher S.
A Myosin Phosphatase Targeting Subunit Isoform Transition Defines a Smooth Muscle Developmental Phenotypic Switch ,
Am J Physiol/Cell
 278(3) , C589 , 2000
Cells
Gizzard and aorta smooth muscle
Enzymes
Medium
HBSS
Muscle
Chicken
Chick
Chamley-Campbell, J. , Campbell, G. and Ross, R.
The Smooth Muscle in Cell Culture ,
Physiol Res
 59 , 1 , 1979
Cells
Smooth muscle
Enzymes
Trypsin : 0.05% - 0.1%
Medium
HBSS
Muscle
Chicken
Chick, white leghorn, 12 day
Bullaro, J. and Brookman, D.
Comparison of Skeletal Muscle Monolayer Cultures Initiated With Cells Dissociated by the Vortex and Trypsin Methods ,
In Vitro
 12 , 564 , 1976
Cells
Muscle
Enzymes
Trypsin : 0.25%
Medium
Puck's saline A
Muscle
Chicken
Chick, white leghorn, embryos, 11 day
Tepperman, K. , Morris, G. , Essien, F. and Heywood, S.M.
A Mechanical Dissociation Method For Preparation of Muscle Cell Cultures ,
J Cell Physiol
 86 , 561 , 1975
Cells
Muscle
Enzymes
Trypsin : 0.05%
Medium
Saline G

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.