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Adipose/Fat
Ovine

Ovine

Mitić, R. , Cantoni, F. , Börlin, C. , Post, M. and Jackisch, L.
A Simplified and Defined Serum-free Medium for Cultivating Fat Across Species. , iScience Vol. 26 , 105822 , 2023
Cells
Stomal vascular cells
Medium
Growth medium
Bone
Chicken
Chick, day old
Gay, C. , Lloyd, Q. and Gilman, V.
Characteristics and Culture of Osteoblasts Derived From Avian Long Bone ,
In Vitro Cell Dev Biol
 30A , 379 , 1994
Cells
Osteoblasts
Enzymes
Trypsin : 0.03%
Medium
DMEM
Bone
Chicken
Chick, Peterson/Arbor Acre, male, 4 weeks old (Gallus domesticus)
Rosselot, G. , Reginato, A.M. and Leach, R.M.
Development of a Serum-Free System to Study the Effect of Growth Hormone and Insulinlike Growth Factor-1 on Cultured Postembryonic Growth Plate Chondrocytes ,
In Vitro Cell Dev Biol
 28A , 235 , 1992
Cells
Chondrocytes
Enzymes
Trypsin : 0.25%
Medium
Ham's F12
Bone
Chicken
Chick, embryo
Schiltz, J.R. , Mayne R. and Holtzer, H.
The Synthesis of Collagen and Glycosaminoglycans by Dedifferentiated Chondroblasts in Culture ,
Differentiation
 1 , 97 , 1973
Cells
Vertebrae chondroblasts
Enzymes
Trypsin : 0.25%
Medium
Simm's, CMF
Brain
Ovine
Lamb
(also calf)
Poduslo, S. , Miller, K. and McKhann, G.
Metabolic Properties of Maintained Oligodendroglia Purified from Brain ,
J Biol Chem
 253 , 1592 , 1978
Cells
Oligodendroglia
Neural
Enzymes
Trypsin : 0.1%
Medium
(see reference)
Cartilage
Chicken
Chick embryos, HH stage
Wong, M. and Tuan, R.
Nuserum, A Synthetic Serum Replacement, Supports Chondrogenesis of Embryonic Chick Limb Bud Mesenchymal Cells in Micromass Culture ,
In Vitro Cell Dev Biol
 29A , 917 , 1993
Cells
Mesenchymal
Enzymes
Trypsin : 0.1%
Medium
DMEM
(see reference)
Cartilage
Chicken
Chicken, broiler strain, 8-10 weeks
Genge, B. , Wu, L. and Wuthier, R.
Differential Fractionation of Matrix Vesicle Proteins: Further Characterization of the Acidic Phospholipid-dependent Ca2+-Binding Proteins ,
J Biol Chem
 265 , 4703 , 1990
Cells
Matrix vesicles
Epiphyseal growth plate
Enzymes
Trypsin : 0.1%
Medium
Tris-buffered saline
Cartilage
Chicken
Chicken, broiler strain, 8-10 week
Genge, B.R. , Wu, L.N.Y. and Wuthier, R.E.
Identification of Phospholipid-dependent Calcium-binding Proteins as Constituents of Matrix Vesicles ,
J Biol Chem
 264 , 10917 , 1989
Cells
Matrix vesicles
Enzymes
Trypsin : 0.1%
Medium
(see reference)
Cartilage
Chicken
Chick embryos, White Leghorn, 19 day old
Gionti, E. , Capasso, O. and Cancedda, R.
The Culture of Chick Embryo Chondrocytes and the Control of Their Differentiated Functions in Vitro ,
J Biol Chem
 258 (11) , 7190 , 1983
Cells
Fibroblasts
Epithelial-like
Enzymes
Trypsin : 0.25%
Medium
E 199 medium
Cartilage
Chicken
Chick embryos, 19 day old
Capasso, O. , Gionti, E. , Pontarelli, G. , Ambesi-Impiobato, F. , Nitsch, L. , Tajana, G. and Cancedda, R.
The Culture of Chick Embryo Chondrocytes and the Control of Their Differentiated Functions In Vitro ,
Exp Cell Res
 142 , 197 , 1982
Cells
Chondrocytes
Enzymes
Trypsin : 0.75%
Medium
Coon's modified F-12
Cartilage
Chicken
Chick embryos, White Leghorn
Ahrens, P. , Solursh, M. and Reiter, R.
Stage-Related Capacity for Limb Chondrogenesis in Cell Culture ,
Dev Biol
 60 , 69 , 1977
Cells
Wing buds
Enzymes
Trypsin : 0.1%
Medium
Saline G
Cartilage
Ovine
Sheep, 2 month
Kojima Koji, Bonassar Lawrence J, Roy Amit K, Mizuno Hirokazu, Cortiella Joaquin, Vacanti Charles A.
A Composite Tissue-Engineered Trachea Using Sheep Nasal Chondrocyte and Epithelial Cells ,
FASEB J
 17 , 823-8 , 2003
Cells
Chondrocytes
Enzymes
Medium
Ham's F-12
Cartilage
Rabbit

Rabbit, New Zealand, 2.7 kg

Khalilifar, M. , Eslaminejad, M. , Ghasemzadeh, M. , Hosseini, S. and Baharvand, H.
In Vitro and In Vivo Comparison of Different Types of Rabbit Mesenchymal Stem Cells for Cartilage Repair. , Cell J Vol. 21 , 150-160 , 2019
Cells
Ear mesenchymal stem
Enzymes
Medium
DMEM
Cartilage
Rabbit
Rabbit, New Zealand, 1.2-1.4 kg
Ju, X. , Deng, M. , Ao, Y. , Yu, C. , Wang, J. , Yu, J. , Cui, G. and Hu, Y.
Protective Effect of Sinomenine on Cartilage Degradation and Chondrocytes Apoptosis. ,
Yakugaku Zasshi
 130 , 1053-60 , 2010
Cells
Chondrocytes
Enzymes
Pronase : 0.2%
Medium
DMEM
Cartilage
Rabbit
Rabbit, New Zealand, 1.8-2.3kg
Mehraban F, Tindal MH, Proffitt MM, Moskowitz RW.
Temporal pattern of cysteine endopeptidase (cathepsin B) expression in cartilage and synovium from rabbit knees with experimental osteoarthritis: gene expression in chondrocytes in response to interleukin-1 and matrix depletion ,
Ann Rheum Dis
 56 , 108 , 1997
Cells
Chondrocytes
Enzymes
Medium
Gey's solution
Cartilage
Rabbit
Rabbit, New Zealand, white, 4-6 wk & 22-25 wk
Plaas, A. , Sandy, J. and Kimura, J.
Biosynthesis of Cartilage Proteoglycan & Link Protein Articular Chondrocytes ,
J Biol Chem
 263 , 7560 , 1988
Cells
Chondrocytes
Enzymes
Protease XIV : 5 mg/g of tissue
Medium
Ham's F-12
Cartilage
Rabbit
Rabbit, white, male, 8 weeks
Benya, P.D. , Padilla, S.R. and Nimni, M.E.
The Progeny of Rabbit Articular Chondrocytes Synthesize Collagen Types I and III and Type I Trimer, but Not Type II ,
Biochemistry
 16 , 865 , 1977
Cells
Chondrocytes
Enzymes
Trypsin : 0.2%
Medium
Gey's BSS
Cartilage
Rabbit
Rabbit, New Zealand white or Dutch, 1 week
(also human, newborn)
Schindler, F.H. , Ose, M.A. and Solursh, M.
The Synthesis of Cartilage Collagen by Rabbit and Human Chondrocytes in Primary Cell Culture ,
In Vitro
 12 , 44 , 1976
Cells
Chondrocytes
Enzymes
Trypsin : 0.1%
Medium
Saline G, CMF
Cartilage
Rabbit
Rabbit, New Zealand white, immature, 2.25 - 3.3 Kg
Green, J.R. and William, T.
Articular Cartilage Repair. Behavior of Rabbit Chondrocytes During Tissue Culture and Subsequent Allografting ,
Clin Orthop Relat Res
 , 237 , 1976
Cells
Articular chondrocytes
Hyaline
Enzymes
Trypsin : 0.2%
Medium
Gey's BSS
Cartilage
Rabbit
Rabbit, New Zealand white, male, 250-350 g
Bentley, G. and Greer , R.
Homotransplantation of Isolated Epiphyseal and Articular Cartilage Chondrocytes into Joint Surfaces of Rabbits ,
Nature
 230 , 385 , 1971
Cells
Epiphyseal
Articular cartilage
Enzymes
Trypsin : 0.25%
Medium
Eagle's basal medium
Endothelial
Rabbit
Rabbit
Choi, J. , Ko, M. and Kay, E.
Subcellular Localization of the Expressed 18 kDa FGF-2 Isoform in Corneal Endothelial Cells ,
Mol Vis
 6 , 222 , 2000
Cells
Corneal endothelial cells (CEC)
Enzymes
Hyaluronidase : 0.05%
Medium
DMEM
Endothelial
Rabbit
Rabbit, 2-3Kg
Haley, N. , Shio, H. and Fowler, S.
Characterization of lipid-laden aortic cells from cholesterol-fed rabbits. I. Resolution of aortic cell populations by metrizamide density gradient centrifugation ,
Lab Invest
 37 , 287 , 1977
Cells
Endothelial, aortic
Enzymes
Elastase : 0.2%
Medium
Hanks solution
Epithelial
Chicken
Chick, 5 day old
Simon-Assmann, P. and Kedinger, M
Embryonic Gut-Dissagregated Culture , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12A:3.1 , 1995
Cells
Intestinal mesenchymal and epithelial
Enzymes
Collagenase : 0.03%
Medium
DMEM
Epithelial
Rabbit
Rabbit, New Zealand white, adult
Vidrich, A. , Racindranath, R. , Farsi, K. and Targan, S.
A Method for the Rapid Establishment of Normal Adult Mammalian Colonic Epithelial Cell Cultures ,
In Vitro Cell Dev Biol
 24 (3) , 188 , 1988
Cells
Colon
Enzymes
Medium
PBS
Epithelial
Rabbit
Rabbit, New Zealand white, male, 4-5lb.
Chew, C.S. and Brown,M.R.
Release of Intracellular Ca2+ and Elevation of Inositol Triphosphate by Secretagogues in Parietal and Chief Cells Isolated from Rabbit Gastric Mucosa ,
Biochim Biophys Acta
 888 , 116 , 1986
Cells
Gastric
Parietal and chief
Enzymes
Medium
Sodium phosphate buffer
Epithelial
Rabbit
Rabbit, New Zealand white estrous, female, 4-5 months
Nicosia, S. , Johnson, J. and Streibel, E.
Isolation and Ultrastructure of Rabbit Ovarian Mesothelium(Surface Epithelium) ,
Int J Gynecol Pathol
 3 , 348 , 1984
Cells
Mesothelial and surface epithelial
Ovaries
Enzymes
Trypsin : 0.125%-0.5%
Medium
Medium 199
Epithelial
Rabbit
Rabbit, fetal
Logsdon, C.D. , Bisbee, C.A. , Rutten, M.J. and Machen, T.E.
Fetal Rabbit Gastric Epithelial Cells Cultured on Floating Collagen Gel ,
In Vitro
 18 , 233 , 1981
Cells
Epithelial
Gastric
Enzymes
Medium
HBSS
Eye
Chicken
Chick, embryo, 6 day
Jacob Vanessa, Rothermel Andrae, Wolf Peter, Layer Paul G.
Rhodopsin, violet and blue opsin expressions in the chick are highly dependent on tissue and serum conditions ,
Cell Death Differ
 180 , 159-68 , 2005
Cells
Retinal cells
Enzymes
Trypsin : 0.005%
Medium
DMEM/F12
Eye
Chicken
Chick embryo
Reenstra Wende R, Orlow Daniel L, Svoboda Kathy K H.
ECM-stimulated signaling and actin reorganization in embryonic corneal epithelia are Rho dependent ,
Inv Ophthal Visual Sci
 43 , 3181-9 , 2002
Cells
Corneal epithelia
Enzymes
Collagenase : 0.08%
Trypsin : 0.08%
Medium
HBSS
Eye
Chicken
Chick embryo
(also rat)
Seigel, G.
The Golden Age of Retinal Cell Culture ,
Mol Vis
 5 , 4 , 1999
Cells
Retinal
Enzymes
Trypsin : 0.6%
Medium
DMEM
Eye
Chicken
Chick, embryo, 10-14 day
Moyer, M. , Bullrich, F. and Sheffield, J.
Emergence of Flat Cells From Glia in Stationary Cultures of Embryonic Chick Neural Retina ,
In Vitro Cell Dev Biol
 26 , 1073 , 1990
Cells
Flat, retina
Enzymes
Trypsin : 0.1%
Medium
Tyrode's solution, CMF
Eye
Rabbit
Rabbit, New Zealand, Adult
Lai, J. , Cheng, H. and Ma, D.
Investigation of Overrun-Processed Porous Hyaluronic Acid Carriers in Corneal Endothelial Tissue Engineering ,
PLoS ONE
 10 , 1371 , 2015
Cells
Corneal endothelial
Enzymes
Medium
HBSS
Eye
Rabbit
Rabbit
Hao, M. , Flynn, K. , Nien-Shy, C. , Jester, B. , Winkler, M. , Brown, D. , La Schiazza, O. , Bille, J. and Jester, J.
In Vivo Non Linear Optical (NLO) Imaging in Live Rabbit Eyes Using the Heidelbert Two-Photon Laser Opthalmoscope ,
Exp Eye Res
 91 , 308 , 2010
Cells
Corneal keratocytes
Enzymes
Collagenase : 0.2%
Hyaluronidase : 0.05%
Medium
DMEM
Eye
Rabbit
Rabbit, New Zealand
Stramer Brian M, Kwok Michael G K, Farthing-Nayak Pamela J, Jung Jae-Chang, Fini M Elizabeth, Nayak Ramesh C.
Monoclonal antibody (3G5)-defined ganglioside: cell surface marker of corneal keratocytes ,
Inv Ophthal Visual Sci
 45 , 807-12 , 2004
Cells
Corneal keratocytes
Enzymes
Trypsin : 0.25%
Collagenase : 0.5%
Medium
PBS
Eye
Rabbit
Rabbit, adult
Brockway LM, Zhou Z-H, Bubien JK, Jovov B, Benos DJ, Keyser KT.
Rabbit retinal neurons and glia express a variety of ENaC/DEG subunits ,
Am J Physiol Cell Physiol
 283 , C126-34 , 2002
Cells
Retinal neurons
Enzymes
Papain : 26 u/ml
Medium
DMEM
Eye
Rabbit
Rabbit, New Zealand, adult, male, albino,
2 kg
Johnson-Muller, B. and Gross, J.
Regulation of Corneal Collagenase Production: Epithelial-Stromal Cell Interactions ,
Proc Natl Acad Sci U S A
 75 (9) , 4417 , 1978
Cells
Epithelial
Enzymes
Trypsin : 0.25%
Medium
HBSS/DMEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.