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Tissue References Search Tool

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Adipose/Fat
Fish
Fish, Atlantic salmon
Todorcevic, M. , Vegusdal, A. , Gjoen, T. , Sundvold, H. , Torstensen, B. , Kjaer, M. and Ruyter, B.
Changes in Fatty Acids Metabolism During Differentiation of Atlantic Salmon Preadipocytes; Effects of n-3 and n-9 Fatty Acids. ,
Biochim Biophys Acta
 1781 , 326 , 2008
Cells
Preadipocytes
Enzymes
Medium
HBSS
Adipose/Fat
Gerbil
Gerbil of unknown age
(also rat, hamster, rabbit, lamb, guinea-pig)
Nedergaard, J. and Lindberg, O.
The Brown Fat Cell ,
Int Rev Cytol
 74 , 187 , 1982
Cells
Brown fat
Enzymes
Medium
Bicarbonate buffer
Epithelial
Fish
Shark (Squalus acanthias)
Karnaky, Jr. , Valentich, J.D. , Currie, M.G. , Oehlenschlager, W.F. and Kennedy, M.P.
Atriopeptin Stimulates Chloride Secretion in Cultured Shark Rectal Gland Cells ,
Am J Physiol
 260 , 1125 , 1991
Cells
Rectal gland
Enzymes
Collagenase : 0.2%
Medium
Ringer's solution
Epithelial
Fish
Shark (Squalus acanthias)
Valentich, J.
Primary Cultures of Shark Rectal Gland Epithelial Cells: A Model for Hormone-Sensitive Chloride Transport ,
J Tiss Cul Meth
 13 , 149 , 1991
Cells
Rectal gland
Enzymes
Collagenase : 0.2%
Medium
Ringer's solution
Epithelial
Fish
Winter flounder, 200-500 g
(Pseudopleuronectes americanus)
Dickman, K. and Renfro, J.
Primary Culture of Flounder Renal Tubule Cells:Transepithelial Transport ,
Am J Physiol
 251 , 424 , 1986
Cells
Renal tubule
Enzymes
Trypsin : 0.2%
Trypsin : 0.2%
Medium
CMF solution
Eye
Fish

Zebrafish, Danio rerio

Middel, C. , Dietrich, N. , Hammes, H. and Kroll, J.
Analysis of the Morphology of Retinal Vascular Cells in Zebrafish , Front Cell Dev Biol Vol. 11 , 1267232 , 2023
Cells
Retinal vascular
Enzymes
Trypsin : 0.3%
Medium
PBS
Eye
Fish
Fish, zebrafish
Diekmann, H. , Kalbhen, P. and Fischer, D.
Characterization of Optic Nerve Regeneration using Transgenic Zebrafish. ,
Front Cell Neurosci
 9 , 118 , 2015
Cells
Retinal
Enzymes
Papain : 10 u/ml
Medium
L-15
Eye
Fish
Goldfish
Graffe, M. , Zenisek, D. and Taraska, J.
A Marginal Band of Microtubules Transports and Organizes Mitochondria in Retinal Bipolar Synaptic Terminals. ,
J Gen Physiol
 146 , 109-17 , 2015
Cells
Retinal Bipolar
Enzymes
Papain : 30 u/ml
Medium
HEPES
Heart
Fish
Zebrafish
Fowler, E. , Wang, N. , Hezzell, M. , Chanoit, G. , Hancox, J. and Cannell, M.
Arrhythmogenic Late Ca2+ Sparks in Failing Heart Cells and Their Control by Action Potential Configuration ,
Proc Natl Acad Sci U S A
 117 , 2687-2692 , 2020
Cells
Cardiac Myocytes
Enzymes
Trypsin : 0.08%
Protease XIV : 0.005%
Collagenase Type 1 : 0.1-0.2%
Medium
HEPES
Heart
Fish
Fish, Danio rerio
Sander, V. , Sune, G. , Jopling, C. , Morera, C. and Belmonte J.
Isolation and In Vitro Culture of Primary Cardiomyocytes from Adult Zebrafish Hearts. ,
Nat Protoc
 8 , 800-9 , 2013
Cells
Cardiomyocytes
Enzymes
Medium
MEM
Kidney
Fish
Winter flounder (Pseudopleur-onectes americanus)
200 - 500 g
Dickman, K. and Renfro, J.
Primary Culture of Flounder Renal Tubule Cells:Transepithelial Transport ,
Am J Physiol
 251 , 424 , 1986
Cells
Renal tubule
Enzymes
Trypsin : 0.2%
Trypsin : 0.2%
Medium
CMF solution
Liver
Fish
Rainbow trout (Oncorhynchus mykiss), male & female,
120-600 g
Blair, J.B. , Miller, M.R. , Pack, D. , Barnes, R. , Teh, S.J. and Hinton, D.E.
Isolated Trout Liver Cells:Establishing Short-Term Primary Cultures Exhibiting Cell-to-Cell Interactions ,
In Vitro Cell Dev Biol
 26 , 237 , 1990
Cells
Hepatocytes
Enzymes
Medium
Kreb's-Ringer bicarbonate buffer, CF
Liver
Fish
Trout, male, 100 - 200 g
Lipsky, M. , Sheridan, T. , Bennett, R. and May, E.
Comparison of Trout Hepatocyte Culture on Different Substrates ,
In Vitro Cell Dev Biol
 22 , 360 , 1986
Cells
Hepatocytes
Enzymes
Medium
HBSS with 0.05M HEPES
Liver
Fish
Rainbow trout, (Salmo gairdneri), male, 150-200 g
Klaunig, J. , Ruch, R. and Goldblah, P.
Trout Hepatocyte Culture: Isolation and Primary Culture ,
In Vitro Cell Dev Biol
 21 , 221 , 1985
Cells
Hepatocytes
Enzymes
Collagenase : 100 u/ml
Medium
HBSS/CMF
Liver
Fish
Trout 100g - 5kg
Bailey, G. , Taylor, M. and Selivonchick, D.
Aflatoxin B1 Metabolism and DNA Binding in Isolated Hepatocytes From Rainbow Trout Salmo gairdner ,
Carcinogenesis
 3 , 511 , 1982
Cells
Hepatocytes
Enzymes
Hyaluronidase : 0.08%
Medium
See reference
Liver
Fish
Trout (Salmo gairdneri), male, 150-200 g
Catfish (Ictalurus punctatus), male, 100 - 150 g
Klaunig, J.
Establishment of Fish Hepatocyte Cultures For Use in In Vitro Carcinogenicity Studies ,
Natl Cancer Inst Monogr
 65 , 163 , 1981
Cells
Hepatocytes
Enzymes
Collagenase : 100u/ml
Medium
HBSS
Muscle
Fish
Zebrafish
Alexander, M. , Kawahara, G. , Kho, A. , Howell, M. , Pusack, T. , Myers, J. , Montanaro, F. , Zon, L. , Guyon, J. and Kunkel, L.
Isolation and Transcriptome Analysis of Adult Zebrafish Cells Enriched for Skeletal Muscle Progenitors. ,
Muscle Nerve
 43 , 741-50 , 2011
Cells
Myogenic progenitor
Enzymes
Medium
PBS
Muscle
Fish
Dogfish, Squalus acanthias , 2-6 Kg
(also guinea-pig, rabbit, frog)
Mitra, R. and Morad, M.
A Uniform Enzymatic Method for Dissociation of Myocytes from Hearts and Stomachs of Vertebrates ,
Am J Physiol
 249 , H1056 , 1985
Cells
Myocytes, heart and stomach
Enzymes
Protease XIV : 0.028%
Medium
Solution C
(see reference)
Neural
Fish
Zebrafish, embryonic
Spiro, Z. , Koh, A. , Tay, S. , See, K. and Winkler, C.
Transcriptional Enhancement of Smn Levels in Motoneurons is Crucial for Proper Axon Morphology in Zebrafish. ,
Sci Rep
 6 , 27470 , 2016
Cells
Motorneurons
Enzymes
PDS kit : per instructions
Medium
L-15
Neural
Fish
Zebrafish
Sakowski, S. , Lunn, J. , Busta, A. , Palmer, M. , Dowling, J. and Feldman, E.
A Novel Approach to Study Motor Neurons from Zebrafish Embryos and Larvae in Culture. ,
J Neurosci Methods
 205 , 277-82 , 2012
Cells
Motor neurons
Enzymes
Medium
See reference
Neural
Fish
Zebrafish, Dania rerio, embryo
Won, Y. , Ono, F. and Ikeda, S.
Identification and Modulation of Voltage-Gated Ca2+ Currents in Zebrafish Rohon-Beard Neurons. ,
J Neurophysiol
 105 , 442-53 , 2011
Cells
Rohon-Beard neurons
Enzymes
Trypsin : 0.2%
Medium
L-15/Hepes
Neural
Fish
Zebrafish, Danio rerio, embryo
Cerda, G. , Hargrave, M. and Lewis, K.
RNA Profiling of FAC-sorted Neurons from the Developing Zebrafish Spinal Cord. ,
Dev Dyn
 238 , 150-61 , 2009
Cells
Neurons
Enzymes
PDS kit : with modifications
Medium
EBSS
Neural
Fish
Black ghose knige fish, adult (Apteronotus albifrons)
Anderson, M.J.
Differences in Growth of Neurons from Normal and Regenerated Teleost Spinal Cord in vitro ,
In Vitro Cell Dev Biol
 29A , 145 , 1993
Cells
Neurons, spinal cord
Enzymes
Trypsin : 0.4%
Medium
PBS, CMF
Pancreas
Fish
Fish, Osphronemus gourami, 3-month-old, 0.5 mg
Schrezenmeir, J. , Laue, C. , Sternheim,E. , Wolbert, K. , Darquy, S. , Chicheportiche, D. , Kirchgessner, J. and Reach, G.
Long-Term Function of Single-Cell Preparations of Piscine Principal Islets in Hollow Fibers ,
Transplant Proc
 24 (6) , 2941 , 1992
Cells
Islets
Enzymes
Collagenase : 0.12 - 0.46 u/ml
Medium
RPMI 1640
Reproductive
Fish
Sebastes Schlegel
Ryu, J. , Kim, H. , Bae, S. , Jung, C. and Gong, S.
Isolation and in Vitro Culture of Primary Cell Populations Derived from Ovarian Tissues of the Rockfish, Sebastes schlegeli ,
Fish Aquatic Sci
 19 , 2016
Cells
Ovarian
Enzymes
Collagenase Type 1 : 500 u/ml
Medium
L-15
Scales
Fish
Goldfish (Carassius Auratus L.)
Lo, S. , Grabowski, S. , Lynch, T. , Kern, D. , Taylor, J.T. and Chen, T.
Isolation of xanthophores from the goldfish ,
In Vitro
 18 , 356 , 1982
Cells
Pigment, xanthopores
Enzymes
Medium
Medium 199 w/BSA

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.