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Tissue References Search Tool

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Adipose/Fat
Avian
Scherpelz, K. , Lu, J.,Tycko, R. and Meredith, S.
Preparation of Amyloid Fibrils Seeded from Brain and Meninges ,
Methods Mol Biol
 1345 , 299 , 2016
Cells
Amyloid Fibrils
Enzymes
Medium
50mM Tris
Adipose/Fat
Avian
Scherpelz, K. , Lu, J.,Tycko, R. and Meredith, S.
Preparation of Amyloid Fibrils Seeded from Brain and Meninges ,
Methods Mol Biol
 1345 , 299 , 2016
Cells
Amyloid Fibrils
Enzymes
Medium
50mM Tris
Adipose/Fat
Fish
Fish, Atlantic salmon
Todorcevic, M. , Vegusdal, A. , Gjoen, T. , Sundvold, H. , Torstensen, B. , Kjaer, M. and Ruyter, B.
Changes in Fatty Acids Metabolism During Differentiation of Atlantic Salmon Preadipocytes; Effects of n-3 and n-9 Fatty Acids. ,
Biochim Biophys Acta
 1781 , 326 , 2008
Cells
Preadipocytes
Enzymes
Medium
HBSS
Adipose/Fat
Hamster
Hamster, adult
(also rat, gerbil, rabbit, lamb, guinea-pig)
Nedergaard, J. and Lindberg, O.
The Brown Fat Cell ,
Int Rev Cytol
 74 , 187 , 1982
Cells
Brown fat
Enzymes
Medium
Bicarbonate buffer
Adipose/Fat
Hamster
Hamster, 5 week-12 month
DiGirolamo, M. , Mendlinger, S. and Fertig, J.W.
A Simple Method to Determine Fat Cell Size and Number in Four Mammalian Species ,
Am J Physiol
 221 , 850 , 1971
Cells
White fat
Enzymes
Collagenase : 0.05%
Medium
Kreb's Ringer bicarbonate buffer
Adrenal
Hamster
Hamster (Mesocricetus auratus) 100-150 g
Liang, B.T. and Perlman, R.L.
Catecholamine Secretion by Hamster Adrenal Cells ,
J Neurochem
 32 , 927 , 1979
Cells
Adrenal
Chromaffin
Enzymes
Hyaluronidase : 0.20%
Medium
Kreb's Ringer bicarbonate buffer
Epithelial
Fish
Shark (Squalus acanthias)
Karnaky, Jr. , Valentich, J.D. , Currie, M.G. , Oehlenschlager, W.F. and Kennedy, M.P.
Atriopeptin Stimulates Chloride Secretion in Cultured Shark Rectal Gland Cells ,
Am J Physiol
 260 , 1125 , 1991
Cells
Rectal gland
Enzymes
Collagenase : 0.2%
Medium
Ringer's solution
Epithelial
Fish
Shark (Squalus acanthias)
Valentich, J.
Primary Cultures of Shark Rectal Gland Epithelial Cells: A Model for Hormone-Sensitive Chloride Transport ,
J Tiss Cul Meth
 13 , 149 , 1991
Cells
Rectal gland
Enzymes
Collagenase : 0.2%
Medium
Ringer's solution
Epithelial
Fish
Winter flounder, 200-500 g
(Pseudopleuronectes americanus)
Dickman, K. and Renfro, J.
Primary Culture of Flounder Renal Tubule Cells:Transepithelial Transport ,
Am J Physiol
 251 , 424 , 1986
Cells
Renal tubule
Enzymes
Trypsin : 0.2%
Trypsin : 0.2%
Medium
CMF solution
Epithelial
Hamster
Hamster, Syrian gold, male, 100-120 g
Niles,R. , Kim, K.C. , Hyman, B. , Christensen, T. , Wasano, K. and Brody, J.
Characterization Of Extended Primary And Secondary Cultures Of Hamster Tracheal Epithelial Cells ,
In Vitro Cell Dev Biol
 24 , 457 , 1988
Cells
Tracheal
Enzymes
Pronase : 0.1%
Medium
MEM with Hepes, CMF
Epithelial
Hamster
Hamster, Syrian golden (strain CR:RGH)
McDowell, E. and et al.
Differentiation of Tracheal Mucociliary Epithelium in Primary Cell Culture Recapitulates Normal Fetal Development and Regeneration Following Injury in Hamsters ,
Am J Pathol
 129 , 511 , 1987
Cells
Tracheal
Enzymes
Trypsin : 0.1%
Medium
Ham's F-12
Epithelial
Hamster
Hamster (strain 1516 EHS and Lakeview), 8-12 weeks
Lee, T. , Wu, R. , Brody, A. , Barrett, J. and Nettesheim, P.
Growth and Differentiation of Hamster Tracheal Epithelial Cells in Culture ,
Exp Lung Res
 6 , 27 , 1984
Cells
Tracheal
Enzymes
Trypsin : 0.05%
Medium
Medium 199
Epithelial
Hamster
Hamster, Syrian gold, male, 6 weeks- 4 months
Goldman, W.E. and Baseman, J.B.
Selective Isolation and Culture of a Proliferating Epithelial Cell Population from the Hamster Trachea ,
In Vitro
 16 , 313 , 1980
Cells
Tracheal
Enzymes
Trypsin : 0.25%
Medium
PBS with EDTA
Eye
Fish

Zebrafish, Danio rerio

Middel, C. , Dietrich, N. , Hammes, H. and Kroll, J.
Analysis of the Morphology of Retinal Vascular Cells in Zebrafish , Front Cell Dev Biol Vol. 11 , 1267232 , 2023
Cells
Retinal vascular
Enzymes
Trypsin : 0.3%
Medium
PBS
Eye
Fish
Fish, zebrafish
Diekmann, H. , Kalbhen, P. and Fischer, D.
Characterization of Optic Nerve Regeneration using Transgenic Zebrafish. ,
Front Cell Neurosci
 9 , 118 , 2015
Cells
Retinal
Enzymes
Papain : 10 u/ml
Medium
L-15
Eye
Fish
Goldfish
Graffe, M. , Zenisek, D. and Taraska, J.
A Marginal Band of Microtubules Transports and Organizes Mitochondria in Retinal Bipolar Synaptic Terminals. ,
J Gen Physiol
 146 , 109-17 , 2015
Cells
Retinal Bipolar
Enzymes
Papain : 30 u/ml
Medium
HEPES
Heart
Fish
Zebrafish
Fowler, E. , Wang, N. , Hezzell, M. , Chanoit, G. , Hancox, J. and Cannell, M.
Arrhythmogenic Late Ca2+ Sparks in Failing Heart Cells and Their Control by Action Potential Configuration ,
Proc Natl Acad Sci U S A
 117 , 2687-2692 , 2020
Cells
Cardiac Myocytes
Enzymes
Trypsin : 0.08%
Protease XIV : 0.005%
Collagenase Type 1 : 0.1-0.2%
Medium
HEPES
Heart
Fish
Fish, Danio rerio
Sander, V. , Sune, G. , Jopling, C. , Morera, C. and Belmonte J.
Isolation and In Vitro Culture of Primary Cardiomyocytes from Adult Zebrafish Hearts. ,
Nat Protoc
 8 , 800-9 , 2013
Cells
Cardiomyocytes
Enzymes
Medium
MEM
Kidney
Avian
Avian, house sparrows
Goldstein D. , Reddy V. and Plaga K.
Second Messenger Production in Avian Medullary Nephron Segments in Response to Peptide Hormones ,
Am J Physiol
 276 , R847 , 1999
Cells
Tubule segments
Enzymes
Medium
HBSS
Kidney
Avian
Avian, chicken
Reich, C. and Bonar, R.
Separation of Avian Kidney Tubules and Glomeruli for In Vitro Culture ,
Meth Cell Sci
 7 , 97 , 1982
Cells
Tubule and glomeruli
Enzymes
Medium
RPMI 1640
Kidney
Fish
Winter flounder (Pseudopleur-onectes americanus)
200 - 500 g
Dickman, K. and Renfro, J.
Primary Culture of Flounder Renal Tubule Cells:Transepithelial Transport ,
Am J Physiol
 251 , 424 , 1986
Cells
Renal tubule
Enzymes
Trypsin : 0.2%
Trypsin : 0.2%
Medium
CMF solution
Kidney
Hamster
Hamster
(also rat, SD and Wistar, 150-225 g, rabbit, bovine)
Husted, R. , Hayashi, M. and Stokes, J.
Characteristics of Papillary Collecting Duct Cells in Primary Culture ,
Am J Physiol
 255 , F1160 , 1988
Cells
Papillary duct
Enzymes
Hyaluronidase : 0.2%
Medium
Keri's buffer
HEPES buffered saline
Liver
Avian
Avian, various
Else, P. , Brand, M. , Turner, N. and Hulbert, A
Respiration Rate of Hepatocytes Varies With Body Mass in Birds ,
J Exp Biol
 207 , 2305 , 2004
Cells
Hepatocytes
Enzymes
Medium
see reference
Liver
Avian
Ducklings, 7 day
Lee J. , Culvenor J. , Angus P. , Smallwood R. , Nicoll A. and Locarnini S.
Duck Hepatitis B Virus Replication in Primary Bile Duct Epithelial Cells ,
J Virol
 75(16) , 7651 , 2001
Cells
Hepatocytes
Enzymes
Collagenase : 0.05%
Hyaluronidase : 0.05%
Medium
DMEM/ F12
Liver
Fish
Rainbow trout (Oncorhynchus mykiss), male & female,
120-600 g
Blair, J.B. , Miller, M.R. , Pack, D. , Barnes, R. , Teh, S.J. and Hinton, D.E.
Isolated Trout Liver Cells:Establishing Short-Term Primary Cultures Exhibiting Cell-to-Cell Interactions ,
In Vitro Cell Dev Biol
 26 , 237 , 1990
Cells
Hepatocytes
Enzymes
Medium
Kreb's-Ringer bicarbonate buffer, CF
Liver
Fish
Trout, male, 100 - 200 g
Lipsky, M. , Sheridan, T. , Bennett, R. and May, E.
Comparison of Trout Hepatocyte Culture on Different Substrates ,
In Vitro Cell Dev Biol
 22 , 360 , 1986
Cells
Hepatocytes
Enzymes
Medium
HBSS with 0.05M HEPES
Liver
Fish
Rainbow trout, (Salmo gairdneri), male, 150-200 g
Klaunig, J. , Ruch, R. and Goldblah, P.
Trout Hepatocyte Culture: Isolation and Primary Culture ,
In Vitro Cell Dev Biol
 21 , 221 , 1985
Cells
Hepatocytes
Enzymes
Collagenase : 100 u/ml
Medium
HBSS/CMF
Liver
Fish
Trout 100g - 5kg
Bailey, G. , Taylor, M. and Selivonchick, D.
Aflatoxin B1 Metabolism and DNA Binding in Isolated Hepatocytes From Rainbow Trout Salmo gairdner ,
Carcinogenesis
 3 , 511 , 1982
Cells
Hepatocytes
Enzymes
Hyaluronidase : 0.08%
Medium
See reference
Liver
Fish
Trout (Salmo gairdneri), male, 150-200 g
Catfish (Ictalurus punctatus), male, 100 - 150 g
Klaunig, J.
Establishment of Fish Hepatocyte Cultures For Use in In Vitro Carcinogenicity Studies ,
Natl Cancer Inst Monogr
 65 , 163 , 1981
Cells
Hepatocytes
Enzymes
Collagenase : 100u/ml
Medium
HBSS
Muscle
Fish
Zebrafish
Alexander, M. , Kawahara, G. , Kho, A. , Howell, M. , Pusack, T. , Myers, J. , Montanaro, F. , Zon, L. , Guyon, J. and Kunkel, L.
Isolation and Transcriptome Analysis of Adult Zebrafish Cells Enriched for Skeletal Muscle Progenitors. ,
Muscle Nerve
 43 , 741-50 , 2011
Cells
Myogenic progenitor
Enzymes
Medium
PBS
Muscle
Fish
Dogfish, Squalus acanthias , 2-6 Kg
(also guinea-pig, rabbit, frog)
Mitra, R. and Morad, M.
A Uniform Enzymatic Method for Dissociation of Myocytes from Hearts and Stomachs of Vertebrates ,
Am J Physiol
 249 , H1056 , 1985
Cells
Myocytes, heart and stomach
Enzymes
Protease XIV : 0.028%
Medium
Solution C
(see reference)
Muscle
Hamster
Hamster, male, 60-70 day
Nakamura, T. , Iwata, Y. , Sampaolesi, M. , Hanada, H. , Saito, N. , Artman, M. , Coetzee, W. and Shigekawa, M.
Stretch-Activated Cation Channels in Skeletal Muscle Myotubes From Sarcoglycan-Deficient Hamsters ,
Am J Physiol Cell Physiol
 281 , C690 , 2001
Cells
Satellite
Enzymes
Trypsin : 0.25%
Medium
DMEM
Neural
Avian
Quail and Chicken, embryonic
Rollo, B. , Zhang, D. , Simkin, J. , Menheniott, T. and Newgreen, D.
Why are Enteric Ganglia so Small? Role of Differential Adhesion of Enteric Neurons and Enteric Neural Crest Cells. ,
F1000Res
 4 , 113 , 2015
Cells
Midgut DRG
Enzymes
Medium
Ham's F12
Neural
Avian
Chick, White Leghorn, embryos
Temburni, M. , Rosenberg, M. , Pathak, N. , McConnell, R. and Jacob, M.
Neuronal Nicotinic Synapse Assembly Requires the Adenomatous Polyposis Coli Tumor Suppressor Protein. ,
J Neurosci
 24 , 6776 , 2004
Cells
Cilary ganglion neurons
Enzymes
Medium
PBS
Neural
Fish
Zebrafish, embryonic
Spiro, Z. , Koh, A. , Tay, S. , See, K. and Winkler, C.
Transcriptional Enhancement of Smn Levels in Motoneurons is Crucial for Proper Axon Morphology in Zebrafish. ,
Sci Rep
 6 , 27470 , 2016
Cells
Motorneurons
Enzymes
PDS kit : per instructions
Medium
L-15
Neural
Fish
Zebrafish
Sakowski, S. , Lunn, J. , Busta, A. , Palmer, M. , Dowling, J. and Feldman, E.
A Novel Approach to Study Motor Neurons from Zebrafish Embryos and Larvae in Culture. ,
J Neurosci Methods
 205 , 277-82 , 2012
Cells
Motor neurons
Enzymes
Medium
See reference

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.